P93306 (NDUS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
NADH dehydrogenase subunit 7

Gene names

Name:	NAD7
Ordered Locus Names:	AtMg00510

Encoded on

Mitochondrion

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme.
Catalytic activity	NADH + ubiquinone = NAD+ + ubiquinol. NADH + acceptor = NAD+ + reduced acceptor.
Subunit structure	Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.
Subcellular location	Mitochondrion.
Miscellaneous	A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07709) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.
Sequence similarities	Belongs to the complex I 49 kDa subunit family.
RNA editing	Edited at positions 13, 26, 46, 67, 82, 84, 106, 112, 115, 193, 233, 242, 245, 247, 257, 353, 360, 363, 368 and 375. Ref.2

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Mitochondrion
Coding sequence diversity	RNA editing
Ligand	NAD Ubiquinone
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	mitochondrial electron transport, NADH to ubiquinone Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	NAD binding Inferred from electronic annotation. Source: InterPro NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC quinone binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	NADH dehydrogenase [ubiquinone] iron-sulfur protein 2		PRO_0000118583

Sequences

	Sequence		Length	Mass (Da)
	P93306 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 6DE66B889FAA6BD0	FASTA	394	44,962
	10 20 30 40 50 60 MTTRKRQIKN FTLNFGPQHP AAHGVLRLVL EMNGEVVERA EPHIGLLHRG TEKLIEYKTY 70 80 90 100 110 120 LQALPYFDRS DYVSMMAQEH AYSLAVEKLL NCEVPLRAQY IRVLFCEITR ILNHLLALTT 130 140 150 160 170 180 HAMDVGALTP FLWAFEEREK LLEFYERVSG ARMHASFIRP GGVAQDLPLG LCRDIDSFTQ 190 200 210 220 230 240 QFASRIDELE EMLTGNRIWK QRLVDIGTVT AQQAKDWGFS GVMLRGPGVC WDLRRAAPYD 250 260 270 280 290 300 VYDQLDFDVP VGTRGDCYDR YCIRIEEMRQ SLRIIVQCLN QMPSGMIKAD DRKLCPPSRC 310 320 330 340 350 360 RMKLSMESSI HHFELYTEGF SVPASSTYTA VEAPKGEFGV FLVSNGSNRP YRCKIRAPGF 370 380 390 AHLQGLDFMS KHHMLADVVT IIGTQDIVFG EVDR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides." Unseld M., Marienfeld J.R., Brandt P., Brennicke A. Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs." Giege P., Brennicke A. Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07709). Strain: cv. Columbia.
[4]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08501 Genomic DNA. Translation: CAA69735.3. Sequence problems. AC007729 Genomic DNA. No translation available.
IPI	IPI00518390.
RefSeq	NP_085511.1. NC_001284.2.
3D structure databases
ProteinModelPortal	P93306.
SMR	P93306. Positions 12-394.
ModBase	Search...
Protein-protein interaction databases
IntAct	P93306. 4 interactions.
Protein family/group databases
TCDB	3.D.1.6.3. H+ or Na+-translocating NADH dehydrogenase (NDH) family.
Proteomic databases
PaxDb	P93306.
PRIDE	P93306.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	814592.
KEGG	ath:ArthMp043.
Organism-specific databases
TAIR	AtMg00510.
Phylogenomic databases
eggNOG	COG0649.
HOGENOM	HOG000228264.
KO	K03935.
Enzyme and pathway databases
BioCyc	ARA:ATMG00510-MONOMER. MetaCyc:ATMG00510-MONOMER.
Gene expression databases
ArrayExpress	P93306.
Genevestigator	P93306.
Family and domain databases
InterPro	IPR001135. NADH_Q_OxRdtase_suD. IPR014029. NADH_UbQ_OxRdtase_49kDa_CS. IPR022885. NDH1_su_D/H. [Graphical view]
Pfam	PF00346. Complex1_49kDa. 1 hit. [Graphical view]
TIGRFAMs	TIGR01962. NuoD. 1 hit.
PROSITE	PS00535. COMPLEX1_49K. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS2_ARATH

Accession

Primary (citable) accession number: P93306

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program