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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 2

Gene

NAD7

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). Component of the iron-sulfur (IP) fragment of the enzyme.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Enzyme and pathway databases

BioCyciARA:ATMG00510-MONOMER.
MetaCyc:ATMG00510-MONOMER.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
NADH dehydrogenase subunit 7
Gene namesi
Name:NAD7
Ordered Locus Names:AtMg00510
Encoded oniMitochondrion
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Mitochondrion

Organism-specific databases

TAIRiATMG00510.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 394394NADH dehydrogenase [ubiquinone] iron-sulfur protein 2PRO_0000118583Add
BLAST

Proteomic databases

PaxDbiP93306.
PRIDEiP93306.

Interactioni

Subunit structurei

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Protein-protein interaction databases

IntActiP93306. 4 interactions.
STRINGi3702.ATMG00510.1.

Structurei

3D structure databases

ProteinModelPortaliP93306.
SMRiP93306. Positions 12-394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I 49 kDa subunit family.Curated

Phylogenomic databases

eggNOGiCOG0649.
HOGENOMiHOG000228264.
InParanoidiP93306.
KOiK03935.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93306-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRKRQIKN FTLNFGPQHP AAHGVLRLVL EMNGEVVERA EPHIGLLHRG
60 70 80 90 100
TEKLIEYKTY LQALPYFDRS DYVSMMAQEH AYSLAVEKLL NCEVPLRAQY
110 120 130 140 150
IRVLFCEITR ILNHLLALTT HAMDVGALTP FLWAFEEREK LLEFYERVSG
160 170 180 190 200
ARMHASFIRP GGVAQDLPLG LCRDIDSFTQ QFASRIDELE EMLTGNRIWK
210 220 230 240 250
QRLVDIGTVT AQQAKDWGFS GVMLRGPGVC WDLRRAAPYD VYDQLDFDVP
260 270 280 290 300
VGTRGDCYDR YCIRIEEMRQ SLRIIVQCLN QMPSGMIKAD DRKLCPPSRC
310 320 330 340 350
RMKLSMESSI HHFELYTEGF SVPASSTYTA VEAPKGEFGV FLVSNGSNRP
360 370 380 390
YRCKIRAPGF AHLQGLDFMS KHHMLADVVT IIGTQDIVFG EVDR
Length:394
Mass (Da):44,962
Last modified:March 1, 2004 - v2
Checksum:i6DE66B889FAA6BD0
GO

RNA editingi

Edited at positions 13, 26, 46, 67, 82, 84, 106, 112, 115, 193, 233, 242, 245, 247, 257, 353, 360, 363, 368 and 375.1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08501 Genomic DNA. Translation: CAA69735.3. Sequence problems.
AC007729 Genomic DNA. No translation available.
RefSeqiNP_085511.1. NC_001284.2.

Genome annotation databases

GeneIDi814592.
KEGGiath:ArthMp043.

Keywords - Coding sequence diversityi

RNA editing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08501 Genomic DNA. Translation: CAA69735.3. Sequence problems.
AC007729 Genomic DNA. No translation available.
RefSeqiNP_085511.1. NC_001284.2.

3D structure databases

ProteinModelPortaliP93306.
SMRiP93306. Positions 12-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP93306. 4 interactions.
STRINGi3702.ATMG00510.1.

Protein family/group databases

TCDBi3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP93306.
PRIDEiP93306.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi814592.
KEGGiath:ArthMp043.

Organism-specific databases

TAIRiATMG00510.

Phylogenomic databases

eggNOGiCOG0649.
HOGENOMiHOG000228264.
InParanoidiP93306.
KOiK03935.

Enzyme and pathway databases

BioCyciARA:ATMG00510-MONOMER.
MetaCyc:ATMG00510-MONOMER.

Miscellaneous databases

PROiP93306.

Family and domain databases

Gene3Di1.10.645.10. 1 hit.
HAMAPiMF_01358. NDH1_NuoD.
InterProiIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
IPR029014. NiFe_Hase-like.
[Graphical view]
PfamiPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
SUPFAMiSSF56762. SSF56762. 1 hit.
TIGRFAMsiTIGR01962. NuoD. 1 hit.
PROSITEiPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides."
    Unseld M., Marienfeld J.R., Brandt P., Brennicke A.
    Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs."
    Giege P., Brennicke A.
    Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07709).
    Strain: cv. Columbia.
  4. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.

Entry informationi

Entry nameiNDUS2_ARATH
AccessioniPrimary (citable) accession number: P93306
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 1, 2004
Last modified: June 24, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07709) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.