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P93306 (NDUS2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 2

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
NADH dehydrogenase subunit 7
Gene names
Name:NAD7
Ordered Locus Names:AtMg00510
Encoded onMitochondrion
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length394 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. Component of the iron-sulfur (IP) fragment of the enzyme. HAMAP-Rule MF_01358

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out). HAMAP-Rule MF_01358

NADH + acceptor = NAD+ + reduced acceptor. HAMAP-Rule MF_01358

Subunit structure

Complex I is composed of at least 49 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.

Subcellular location

Mitochondrion Ref.4.

Miscellaneous

A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of the duplicated gene (At2g07709) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.

Sequence similarities

Belongs to the complex I 49 kDa subunit family.

RNA editing

Edited at positions 13, 26, 46, 67, 82, 84, 106, 112, 115, 193, 233, 242, 245, 247, 257, 353, 360, 363, 368 and 375. Ref.2

Ontologies

Keywords
   Biological processElectron transport
Respiratory chain
Transport
   Cellular componentMitochondrion
   Coding sequence diversityRNA editing
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrion

Inferred from electronic annotation. Source: UniProtKB-SubCell

respiratory chain

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: UniProtKB-EC

quinone binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 394394NADH dehydrogenase [ubiquinone] iron-sulfur protein 2 HAMAP-Rule MF_01358
PRO_0000118583

Sequences

Sequence LengthMass (Da)Tools
P93306 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 6DE66B889FAA6BD0

FASTA39444,962
        10         20         30         40         50         60 
MTTRKRQIKN FTLNFGPQHP AAHGVLRLVL EMNGEVVERA EPHIGLLHRG TEKLIEYKTY 

        70         80         90        100        110        120 
LQALPYFDRS DYVSMMAQEH AYSLAVEKLL NCEVPLRAQY IRVLFCEITR ILNHLLALTT 

       130        140        150        160        170        180 
HAMDVGALTP FLWAFEEREK LLEFYERVSG ARMHASFIRP GGVAQDLPLG LCRDIDSFTQ 

       190        200        210        220        230        240 
QFASRIDELE EMLTGNRIWK QRLVDIGTVT AQQAKDWGFS GVMLRGPGVC WDLRRAAPYD 

       250        260        270        280        290        300 
VYDQLDFDVP VGTRGDCYDR YCIRIEEMRQ SLRIIVQCLN QMPSGMIKAD DRKLCPPSRC 

       310        320        330        340        350        360 
RMKLSMESSI HHFELYTEGF SVPASSTYTA VEAPKGEFGV FLVSNGSNRP YRCKIRAPGF 

       370        380        390 
AHLQGLDFMS KHHMLADVVT IIGTQDIVFG EVDR 

« Hide

References

« Hide 'large scale' references
[1]"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides."
Unseld M., Marienfeld J.R., Brandt P., Brennicke A.
Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs."
Giege P., Brennicke A.
Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07709).
Strain: cv. Columbia.
[4]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y08501 Genomic DNA. Translation: CAA69735.3. Sequence problems.
AC007729 Genomic DNA. No translation available.
RefSeqNP_085511.1. NC_001284.2.

3D structure databases

ProteinModelPortalP93306.
SMRP93306. Positions 12-394.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP93306. 4 interactions.

Protein family/group databases

TCDB3.D.1.6.3. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbP93306.
PRIDEP93306.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID814592.
KEGGath:ArthMp043.

Organism-specific databases

TAIRATMG00510.

Phylogenomic databases

eggNOGCOG0649.
HOGENOMHOG000228264.
KOK03935.

Enzyme and pathway databases

BioCycARA:ATMG00510-MONOMER.
MetaCyc:ATMG00510-MONOMER.

Gene expression databases

GenevestigatorP93306.

Family and domain databases

HAMAPMF_01358. NDH1_NuoD.
InterProIPR001135. NADH_Q_OxRdtase_suD.
IPR014029. NADH_UbQ_OxRdtase_49kDa_CS.
IPR022885. NDH1_su_D/H.
[Graphical view]
PfamPF00346. Complex1_49kDa. 1 hit.
[Graphical view]
TIGRFAMsTIGR01962. NuoD. 1 hit.
PROSITEPS00535. COMPLEX1_49K. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS2_ARATH
AccessionPrimary (citable) accession number: P93306
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: March 1, 2004
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names