P93285 (COX2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 2
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide II

Gene names

Name:	COX2
Synonyms:	COXII
Ordered Locus Names:	AtMg00160

Encoded on

Mitochondrion

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1 By similarity.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Cofactor	Copper A.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 2 family.
RNA editing	Edited at positions 9, 24, 85, 93, 127, 159, 186, 194, 233, 241 and 248. Ref.2

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Ligand	Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	Cytochrome c oxidase subunit 2		PRO_0000183502
Regions
	Topological domain	1 – 41	41	Mitochondrial intermembrane Potential
	Transmembrane	42 – 62	21	Helical; Potential
	Topological domain	63 – 86	24	Mitochondrial matrix Potential
	Transmembrane	87 – 107	21	Helical; Potential
	Topological domain	108 – 260	153	Mitochondrial intermembrane Potential
Sites
	Metal binding	187	1	Copper A By similarity
	Metal binding	222	1	Copper A By similarity
	Metal binding	226	1	Copper A By similarity
	Metal binding	230	1	Copper A By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93285 [UniParc]. Last modified March 1, 2004. Version 2. Checksum: 7D2D75A093973985	FASTA	260	29,674
	10 20 30 40 50 60 MIVLKWLFFT ISPCDAAEPW QLGFQDAATP IMQGIIDLHH DIFFFLILIL VFVLWILVRA 70 80 90 100 110 120 LWHFHYKKNA IPQRIVHGTT IEILWTIFPS IILMFIAIPS FALLYSMDEV VVDPAITIKA 130 140 150 160 170 180 IGHQWYWTYE YSDYNSSDEQ SLTFDSYMIP EEDLELGQLR LLEVDNRVVV PAKTHLRIIV 190 200 210 220 230 240 TSADVLHSWA VPSLGVKCDA VPGRLNQISI LVQREGVYYG QCSEICGTNH AFMSIVVEAV 250 260 SRKDYGSWVS NQLIPQTGEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides." Unseld M., Marienfeld J.R., Brandt P., Brennicke A. Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs." Giege P., Brennicke A. Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING.
[3]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS]. Strain: cv. Landsberg erecta.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08501 Genomic DNA. Translation: CAA69761.3. Sequence problems.
IPI	IPI00529181.
RefSeq	NP_085487.1. NC_001284.2.
3D structure databases
ProteinModelPortal	P93285.
SMR	P93285. Positions 17-253.
ModBase	Search...
Proteomic databases
PaxDb	P93285.
PRIDE	P93285.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	814573.
KEGG	ath:ArthMp015.
Organism-specific databases
TAIR	AtMg00160.
Phylogenomic databases
eggNOG	COG1622.
HOGENOM	HOG000264988.
KO	K02261.
Enzyme and pathway databases
BioCyc	ARA:ATMG00160-MONOMER. MetaCyc:ATMG00160-MONOMER.
Gene expression databases
ArrayExpress	P93285.
Genevestigator	P93285.
GermOnline	ATMG00160. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.287.90. 1 hit. 2.60.40.420. 1 hit.
InterPro	IPR001505. Copper_CuA. IPR008972. Cupredoxin. IPR014222. Cyt_c_oxidase_su2. IPR002429. Cyt_c_oxidase_su2_C. IPR011759. Cyt_c_oxidase_su2_TM_dom. [Graphical view]
Pfam	PF00116. COX2. 1 hit. PF02790. COX2_TM. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF81464. Cyt_c_oxidase_II-like_TM. 1 hit.
TIGRFAMs	TIGR02866. CoxB. 1 hit.
PROSITE	PS00078. COX2. 1 hit. PS50857. COX2_CUA. 1 hit. PS50999. COX2_TM. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX2_ARATH

Accession

Primary (citable) accession number: P93285

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	March 1, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program