Reviewed, UniProtKB/Swiss-Prot P93236 (ABA2_SOLLC)

Names and origin

Protein names	Recommended name:     Zeaxanthin epoxidase, chloroplastic     EC=1.14.13.90
Organism	Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifier	4081 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts zeaxanthin into antheraxanthin and subsequently violaxanthin. Involved in the epoxidation of zeaxanthin. Plays an important role in resistance to stresses, seed development and dormancy.
Catalytic activity	Zeaxanthin + NAD(P)H + O(2) = antheraxanthin + NAD(P)(+) + H(2)O. Antheraxanthin + NAD(P)H + O(2) = violaxanthin + NAD(P)(+) + H(2)O.
Cofactor	FAD Potential.
Pathway	Plant hormone biosynthesis; abscisic acid biosynthesis. Lipid metabolism; steroid biosynthesis.
Subcellular location	Plastid › chloroplastBy similarity.
Sequence similarities	Contains 1 FHA domain.

Ontologies

Keywords
Biological process	Abscisic acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	FAD Flavoprotein NAD NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	abscisic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	zeaxanthin epoxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 49	49	Chloroplast Potential
	Chain	50 – 669	620	Zeaxanthin epoxidase, chloroplastic		PRO_0000020610
Regions
	Domain	553 – 617	65	FHA
	Nucleotide binding	87 – 115	29	FAD Potential
	Nucleotide binding	365 – 378	14	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	P93236-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 1140C9F29C3499B7	FASTA	669	73,102
	10 20 30 40 50 60 MYSTVFYTSV HPSTSVLSRK QLPLLISKDF SAELYHSLPC RSLENGHINK VKGVKVKATI 70 80 90 100 110 120 AEAPVTPTEK TDSGANGDLK VPQKKLKVLV AGGGIGGLVF ALAAKKRGFD VLVFERDLSA 130 140 150 160 170 180 IRGEGQYRGP IQIQSNALAA LEAIDLDVAE DIMNAGCITG QRINGLVDGI SGNWYCKFDT 190 200 210 220 230 240 FTPAVERGLP VTRVISRMTL QQILARAVGE EIIMNESNVV DFEDDGEKVT VVLENGQRFT 250 260 270 280 290 300 GDLLVGADGI RSKVRTNLFG PSEATYSGYT CYTGIADFVP ADIDTVGYRV FLGHKQYFVS 310 320 330 340 350 360 SDVGGGKMQW YAFYNEPAGG ADAPNGKKER LLKIFGGWCD NVIDLLVATD EDAILRRDIY 370 380 390 400 410 420 DRPPTFSWGR GRVTLLGDSV HAMQPNLGQG GCMAIEDSYQ LALELEKACS RSAEFGSPVD 430 440 450 460 470 480 IISSLRSYES ARKLRVGVIH GLARMAAIMA STYKAYLGVG LGPLSFLTQY RIPHPGRVGG 490 500 510 520 530 540 RVFIDLGMPL MLSWVLGGNG DKLEGRIKHC RLSEKANDQL RKWFEDDDAL ERATDAEWLL 550 560 570 580 590 600 LPAGNGSSGL EAIVLSRDED VPCTVGSISH TNIPGKSIVL PLPQVSEMHA RISCKDGAFF 610 620 630 640 650 660 VTDLRSEHGT WVTDNEGRRY RTSPNFPTRF HPSDVIEFGS DKAAFRVKAM KFPLKTSERK EEREAVEAA

References

[1]	"Structure and expression of a cDNA encoding zeaxanthine epoxidase, isolated from a wilt-related tomato (Lycopersicon esculentum Mill.) library." Burbidge A., Grieve T., Terry C., Corlett J., Thompson A., Taylor I. J. Exp. Bot. 48:1749-1750(1997) Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z83835 mRNA. Translation: CAB06084.1.
PIR	T07754.
UniGene	Les.112
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR000253. FHA. IPR003042. Rng_hydrolase. IPR017079. Zeaxanthin_epoxidase. [Graphical view]
Gene3D	G3DSA:2.60.200.20. FHA. 1 hit.
Pfam	PF01266. DAO. 1 hit. PF00498. FHA. 1 hit. [Graphical view]
PIRSF	PIRSF036989. Zeaxanthin_epoxidase. 1 hit.
PRINTS	PR00420. RNGMNOXGNASE.
SMART	SM00240. FHA. 1 hit. [Graphical view]
PROSITE	PS50006. FHA_DOMAIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABA2_SOLLC

Accession

Primary (citable) accession number: P93236

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	November 4, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)