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Protein

Lipoxygenase 2.1, chloroplastic

Gene

LOX2.1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme is possibly involved in jasmonic acid synthesis. It exhibits linoleate 13-lipoxygenase and arachidonate 15-lipoxygenase activity.1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.1 Publication
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit. Iron is tightly bound.PROSITE-ProRule annotation

pH dependencei

Optimum pH is 7.

Pathwayi: oxylipin biosynthesis

This protein is involved in the pathway oxylipin biosynthesis, which is part of Lipid metabolism.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway oxylipin biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi587Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi592Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi777Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi781Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi936Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • linoleate 13S-lipoxygenase activity Source: CACAO
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • oxylipin biosynthetic process Source: UniProtKB-UniPathway
  • response to jasmonic acid Source: CACAO

Keywordsi

Molecular functionDioxygenase, Oxidoreductase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.12 2687
1.13.11.33 2687
UniPathwayiUPA00382

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2.1, chloroplastic (EC:1.13.11.12)
Alternative name(s):
LOX-100
LOX2:Hv:1
Gene namesi
Name:LOX2.1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000018323? – 936Lipoxygenase 2.1, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiP93184

Expressioni

Inductioni

Transient induction by exogenous methyl jasmonate, reaching a maximum 48 hours after treatment. Not induced by endogenous jasmonic acid resulting from sorbitol stress.1 Publication

Gene expression databases

ExpressionAtlasiP93184 baseline and differential

Structurei

3D structure databases

ProteinModelPortaliP93184
SMRiP93184
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini88 – 217PLATPROSITE-ProRule annotationAdd BLAST130
Domaini220 – 936LipoxygenasePROSITE-ProRule annotationAdd BLAST717

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IIW1 Eukaryota
ENOG410YN4N LUCA

Family and domain databases

Gene3Di4.10.372.10, 1 hit
InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001246 LipOase_plant
IPR027433 Lipoxygenase_dom_3
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00468 PLTLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93184-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTATKPLVG GACAAPSSSA RRRTFVVPEA RRKPGNGRRT SVSKVGSTST
60 70 80 90 100
STTTTTTTTL SADSNGAAVG TVTRPDVHVQ DRTHATEMKA TVTVHMSKAA
110 120 130 140 150
GVRDFLYDLI LKTWLHVDLV SSELDPQTGQ EREPISGAVK HSGRVDDEWD
160 170 180 190 200
MYEATFKVPA SFGPIGAVQV TNYHHSEMLL GDIEVFPTGQ EESAVTFHCK
210 220 230 240 250
SWIDPSHCTP DKRVFFPAHS YLPSQTPKGV EGLRKRELEI LRGTGCGERK
260 270 280 290 300
EHDRIYDYDV YNDLGNPDDD NNPTTRPVLG GKEHPYPRRC RTGRPRSKKD
310 320 330 340 350
PFSEERSHKE HIYVPRDEAF TERKMGAFDT KKFMSQLHAL TTGLKTAKHK
360 370 380 390 400
SQSFPSLSAI DQLYDDNFRN QPVQPEGGKL RFVIDLLETE LLHLFKLEGA
410 420 430 440 450
AFLEGIRRVF KFETPEIHDR DKFAWFRDEE FARQTIAGMN PMSIQLVTEF
460 470 480 490 500
PIKSNLDEAT YGPADSLITK EVVEEQIRRV MTADEAVQNK KLFMLDYHDL
510 520 530 540 550
LLPYVHKVRK LDGTTLYGSR ALFFLTADGT LRPIAIELTR PKSKKKPQWR
560 570 580 590 600
QVFTPGCDGS VTGSWLWQLA KAHILAHDAG VHQLVSHWLR THACTEPYII
610 620 630 640 650
AANRQLSQMH PVYRLLHPHF RFTMEINAQA RAMLINAGGI IEGSFVPGEY
660 670 680 690 700
SLELSSVAYD QQWRFDMEAL PEDLIRRGMA VRNPNGELEL AIEDYPYAND
710 720 730 740 750
GLLVWDAIKQ WALTYVQHYY PCAADIVDDE ELQAWWTEVR TKGHADKQDE
760 770 780 790 800
PWWPELDSHE NLAQTLATIM WVTSGHHAAV NFGQYPMAGY IPNRPTMARR
810 820 830 840 850
NMPTEIGGDD MRDFVEAPEK VLLDTFPSQY QSAIVLAILD LLSTHSSDEE
860 870 880 890 900
YMGTHEEPAW TKDGVINQAF EEFKESTRKI VEQVDEWNND PDRKNRHGAG
910 920 930
MVPYVLLRPS DGDPTDGDPT DEKMVMEMGI PNSISI
Length:936
Mass (Da):105,864
Last modified:May 1, 1997 - v1
Checksum:i567B8519E0B5D959
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U56406 mRNA Translation: AAC12951.1
PIRiT06190

Similar proteinsi

Entry informationi

Entry nameiLOX21_HORVU
AccessioniPrimary (citable) accession number: P93184
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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