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P93184

- LOX21_HORVU

UniProt

P93184 - LOX21_HORVU

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Protein
Lipoxygenase 2.1, chloroplastic
Gene
LOX2.1
Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme is possibly involved in jasmonic acid synthesis. It exhibits linoleate 13-lipoxygenase and arachidonate 15-lipoxygenase activity.1 Publication

Catalytic activityi

Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.1 Publication
Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.1 Publication

Cofactori

Binds 1 iron ion per subunit. Iron is tightly bound By similarity.

pH dependencei

Optimum pH is 7.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi587 – 5871Iron; catalytic By similarity
Metal bindingi592 – 5921Iron; catalytic By similarity
Metal bindingi777 – 7771Iron; catalytic By similarity
Metal bindingi781 – 7811Iron; catalytic By similarity
Metal bindingi936 – 9361Iron; via carboxylate; catalytic By similarity

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC

GO - Biological processi

  1. oxylipin biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00382.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoxygenase 2.1, chloroplastic (EC:1.13.11.12)
Alternative name(s):
LOX-100
LOX2:Hv:1
Gene namesi
Name:LOX2.1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiP93184.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 936Lipoxygenase 2.1, chloroplasticPRO_0000018323
Transit peptidei1 – ?Chloroplast Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.

Expressioni

Inductioni

Transient induction by exogenous methyl jasmonate, reaching a maximum 48 hours after treatment. Not induced by endogenous jasmonic acid resulting from sorbitol stress.1 Publication

Gene expression databases

GenevestigatoriP93184.

Structurei

3D structure databases

ProteinModelPortaliP93184.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini88 – 217130PLAT
Add
BLAST
Domaini220 – 936717Lipoxygenase
Add
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.
Contains 1 PLAT domain.

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93184-1 [UniParc]FASTAAdd to Basket

« Hide

MLTATKPLVG GACAAPSSSA RRRTFVVPEA RRKPGNGRRT SVSKVGSTST    50
STTTTTTTTL SADSNGAAVG TVTRPDVHVQ DRTHATEMKA TVTVHMSKAA 100
GVRDFLYDLI LKTWLHVDLV SSELDPQTGQ EREPISGAVK HSGRVDDEWD 150
MYEATFKVPA SFGPIGAVQV TNYHHSEMLL GDIEVFPTGQ EESAVTFHCK 200
SWIDPSHCTP DKRVFFPAHS YLPSQTPKGV EGLRKRELEI LRGTGCGERK 250
EHDRIYDYDV YNDLGNPDDD NNPTTRPVLG GKEHPYPRRC RTGRPRSKKD 300
PFSEERSHKE HIYVPRDEAF TERKMGAFDT KKFMSQLHAL TTGLKTAKHK 350
SQSFPSLSAI DQLYDDNFRN QPVQPEGGKL RFVIDLLETE LLHLFKLEGA 400
AFLEGIRRVF KFETPEIHDR DKFAWFRDEE FARQTIAGMN PMSIQLVTEF 450
PIKSNLDEAT YGPADSLITK EVVEEQIRRV MTADEAVQNK KLFMLDYHDL 500
LLPYVHKVRK LDGTTLYGSR ALFFLTADGT LRPIAIELTR PKSKKKPQWR 550
QVFTPGCDGS VTGSWLWQLA KAHILAHDAG VHQLVSHWLR THACTEPYII 600
AANRQLSQMH PVYRLLHPHF RFTMEINAQA RAMLINAGGI IEGSFVPGEY 650
SLELSSVAYD QQWRFDMEAL PEDLIRRGMA VRNPNGELEL AIEDYPYAND 700
GLLVWDAIKQ WALTYVQHYY PCAADIVDDE ELQAWWTEVR TKGHADKQDE 750
PWWPELDSHE NLAQTLATIM WVTSGHHAAV NFGQYPMAGY IPNRPTMARR 800
NMPTEIGGDD MRDFVEAPEK VLLDTFPSQY QSAIVLAILD LLSTHSSDEE 850
YMGTHEEPAW TKDGVINQAF EEFKESTRKI VEQVDEWNND PDRKNRHGAG 900
MVPYVLLRPS DGDPTDGDPT DEKMVMEMGI PNSISI 936
Length:936
Mass (Da):105,864
Last modified:May 1, 1997 - v1
Checksum:i567B8519E0B5D959
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56406 mRNA. Translation: AAC12951.1.
PIRiT06190.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U56406 mRNA. Translation: AAC12951.1 .
PIRi T06190.

3D structure databases

ProteinModelPortali P93184.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P93184.

Enzyme and pathway databases

UniPathwayi UPA00382 .

Gene expression databases

Genevestigatori P93184.

Family and domain databases

Gene3Di 2.60.60.20. 1 hit.
4.10.372.10. 1 hit.
InterProi IPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001246. LipOase_pln.
IPR027433. Lipoxygenase_domain_3.
IPR001024. PLAT/LH2_dom.
[Graphical view ]
PANTHERi PTHR11771. PTHR11771. 1 hit.
Pfami PF00305. Lipoxygenase. 1 hit.
PF01477. PLAT. 1 hit.
[Graphical view ]
PRINTSi PR00087. LIPOXYGENASE.
PR00468. PLTLPOXGNASE.
SMARTi SM00308. LH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of a methyljasmonate-inducible lipoxygenase from barley (Hordeum vulgare cv. Salome) leaves."
    Voeroes K., Feussner I., Kuehn H., Lee J., Graner A., Loebler M., Parthier B., Wasternack C.
    Eur. J. Biochem. 251:36-44(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 213-227, CATALYTIC ACTIVITY, FUNCTION, INDUCTION.
    Strain: cv. Salome.
    Tissue: Leaf.

Entry informationi

Entry nameiLOX21_HORVU
AccessioniPrimary (citable) accession number: P93184
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: May 1, 1997
Last modified: October 16, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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