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P93184

- LOX21_HORVU

UniProt

P93184 - LOX21_HORVU

Protein

Lipoxygenase 2.1, chloroplastic

Gene

LOX2.1

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. This enzyme is possibly involved in jasmonic acid synthesis. It exhibits linoleate 13-lipoxygenase and arachidonate 15-lipoxygenase activity.1 Publication

    Catalytic activityi

    Linoleate + O2 = (9Z,11E,13S)-13-hydroperoxyoctadeca-9,11-dienoate.1 Publication
    Alpha-linolenate + O2 = (9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate.1 Publication

    Cofactori

    Binds 1 iron ion per subunit. Iron is tightly bound By similarity.PROSITE-ProRule annotation

    pH dependencei

    Optimum pH is 7.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi587 – 5871Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi592 – 5921Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi777 – 7771Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi781 – 7811Iron; catalyticPROSITE-ProRule annotation
    Metal bindingi936 – 9361Iron; via carboxylate; catalyticPROSITE-ProRule annotation

    GO - Molecular functioni

    1. iron ion binding Source: InterPro
    2. linoleate 13S-lipoxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. oxylipin biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Oxylipin biosynthesis

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00382.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoxygenase 2.1, chloroplastic (EC:1.13.11.12)
    Alternative name(s):
    LOX-100
    LOX2:Hv:1
    Gene namesi
    Name:LOX2.1
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiP93184.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 936Lipoxygenase 2.1, chloroplasticPRO_0000018323
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Expressioni

    Inductioni

    Transient induction by exogenous methyl jasmonate, reaching a maximum 48 hours after treatment. Not induced by endogenous jasmonic acid resulting from sorbitol stress.1 Publication

    Gene expression databases

    GenevestigatoriP93184.

    Structurei

    3D structure databases

    ProteinModelPortaliP93184.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini88 – 217130PLATPROSITE-ProRule annotationAdd
    BLAST
    Domaini220 – 936717LipoxygenasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lipoxygenase family.Curated
    Contains 1 lipoxygenase domain.PROSITE-ProRule annotation
    Contains 1 PLAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProiIPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view]
    PANTHERiPTHR11771. PTHR11771. 1 hit.
    PfamiPF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view]
    PRINTSiPR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTiSM00308. LH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P93184-1 [UniParc]FASTAAdd to Basket

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    MLTATKPLVG GACAAPSSSA RRRTFVVPEA RRKPGNGRRT SVSKVGSTST    50
    STTTTTTTTL SADSNGAAVG TVTRPDVHVQ DRTHATEMKA TVTVHMSKAA 100
    GVRDFLYDLI LKTWLHVDLV SSELDPQTGQ EREPISGAVK HSGRVDDEWD 150
    MYEATFKVPA SFGPIGAVQV TNYHHSEMLL GDIEVFPTGQ EESAVTFHCK 200
    SWIDPSHCTP DKRVFFPAHS YLPSQTPKGV EGLRKRELEI LRGTGCGERK 250
    EHDRIYDYDV YNDLGNPDDD NNPTTRPVLG GKEHPYPRRC RTGRPRSKKD 300
    PFSEERSHKE HIYVPRDEAF TERKMGAFDT KKFMSQLHAL TTGLKTAKHK 350
    SQSFPSLSAI DQLYDDNFRN QPVQPEGGKL RFVIDLLETE LLHLFKLEGA 400
    AFLEGIRRVF KFETPEIHDR DKFAWFRDEE FARQTIAGMN PMSIQLVTEF 450
    PIKSNLDEAT YGPADSLITK EVVEEQIRRV MTADEAVQNK KLFMLDYHDL 500
    LLPYVHKVRK LDGTTLYGSR ALFFLTADGT LRPIAIELTR PKSKKKPQWR 550
    QVFTPGCDGS VTGSWLWQLA KAHILAHDAG VHQLVSHWLR THACTEPYII 600
    AANRQLSQMH PVYRLLHPHF RFTMEINAQA RAMLINAGGI IEGSFVPGEY 650
    SLELSSVAYD QQWRFDMEAL PEDLIRRGMA VRNPNGELEL AIEDYPYAND 700
    GLLVWDAIKQ WALTYVQHYY PCAADIVDDE ELQAWWTEVR TKGHADKQDE 750
    PWWPELDSHE NLAQTLATIM WVTSGHHAAV NFGQYPMAGY IPNRPTMARR 800
    NMPTEIGGDD MRDFVEAPEK VLLDTFPSQY QSAIVLAILD LLSTHSSDEE 850
    YMGTHEEPAW TKDGVINQAF EEFKESTRKI VEQVDEWNND PDRKNRHGAG 900
    MVPYVLLRPS DGDPTDGDPT DEKMVMEMGI PNSISI 936
    Length:936
    Mass (Da):105,864
    Last modified:May 1, 1997 - v1
    Checksum:i567B8519E0B5D959
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56406 mRNA. Translation: AAC12951.1.
    PIRiT06190.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U56406 mRNA. Translation: AAC12951.1 .
    PIRi T06190.

    3D structure databases

    ProteinModelPortali P93184.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P93184.

    Enzyme and pathway databases

    UniPathwayi UPA00382 .

    Gene expression databases

    Genevestigatori P93184.

    Family and domain databases

    Gene3Di 2.60.60.20. 1 hit.
    4.10.372.10. 1 hit.
    InterProi IPR008976. Lipase_LipOase.
    IPR000907. LipOase.
    IPR013819. LipOase_C.
    IPR020834. LipOase_CS.
    IPR020833. LipOase_Fe_BS.
    IPR001246. LipOase_pln.
    IPR027433. Lipoxygenase_domain_3.
    IPR001024. PLAT/LH2_dom.
    [Graphical view ]
    PANTHERi PTHR11771. PTHR11771. 1 hit.
    Pfami PF00305. Lipoxygenase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view ]
    PRINTSi PR00087. LIPOXYGENASE.
    PR00468. PLTLPOXGNASE.
    SMARTi SM00308. LH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48484. SSF48484. 1 hit.
    SSF49723. SSF49723. 1 hit.
    PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
    PS00081. LIPOXYGENASE_2. 1 hit.
    PS51393. LIPOXYGENASE_3. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of a methyljasmonate-inducible lipoxygenase from barley (Hordeum vulgare cv. Salome) leaves."
      Voeroes K., Feussner I., Kuehn H., Lee J., Graner A., Loebler M., Parthier B., Wasternack C.
      Eur. J. Biochem. 251:36-44(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 213-227, CATALYTIC ACTIVITY, FUNCTION, INDUCTION.
      Strain: cv. Salome.
      Tissue: Leaf.

    Entry informationi

    Entry nameiLOX21_HORVU
    AccessioniPrimary (citable) accession number: P93184
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 19, 2003
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3