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P93147 (C81E1_GLYEC) Reviewed, UniProtKB/Swiss-Prot

Last modified September 21, 2011. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoflavone 2'-hydroxylase

EC=1.14.13.89
Alternative name(s):
CYP GE-3
Cytochrome P450 81E1
Cytochrome P450 91A4
Gene names
Name:CYP81E1
Synonyms:CYP91A4
OrganismGlycyrrhiza echinata (Licorice)
Taxonomic identifier46348 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsfabidsFabalesFabaceaePapilionoideaeGalegeaeGlycyrrhiza

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the hydroxylation of isoflavones, daidzein and formononetin, to yield 2'-hydroxyisoflavones, 2'-hydroxydaidzein, and 2'-hydroxyformononetin, respectively.

Catalytic activity

An isoflavone + NADPH + O2 = a 2'-hydroxyisoflavone + NADP+ + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Membrane Potential.

Induction

By fungal elicitor.

Miscellaneous

Involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentMembrane
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
Gene Ontology (GO)
   Cellular componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionelectron carrier activity

Inferred from electronic annotation. Source: InterPro

heme binding

Inferred from electronic annotation. Source: InterPro

isoflavone 2'-hydroxylase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Isoflavone 2'-hydroxylase
PRO_0000052160

Sites

Metal binding4361Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P93147 [UniParc].

Last modified January 1, 1998. Version 2.
Checksum: 4D03AFAC818C04DB

FASTA49957,192
        10         20         30         40         50         60 
MEILSLLSYS VFYLALFFIF NIVIRARKFK NLPPGPPSLP IIGNLHHLKR PLHRTFKGLS 

        70         80         90        100        110        120 
EKYGHVFSLW FGSRLVVVVS SASEFQQCFT KNDVVLANRP RFLSGKYIFY NYTTLGSTSY 

       130        140        150        160        170        180 
GEHWRNLRRI TALDVLSNHR INSFSGIRRD ETQRLITRLA DDSSTNFAEM ELSSRLYDMT 

       190        200        210        220        230        240 
FNNIMRMISG KRYYGEDCDT SDLQEASQFR DMVSELLQLS GANNKTDFMP LLRFLDFENL 

       250        260        270        280        290        300 
EKRLKDISGK TDAFLRGLIE EHRTKKERAN TMIDHLLNLQ DSQPEYYTDQ IIKGLALAML 

       310        320        330        340        350        360 
LAGTDSSAVT LEWSMSNLLN HPEVLKKVKD ELDTHVGQDR LVDESDLPKL TYLKNVINET 

       370        380        390        400        410        420 
LRLYTPAPLL LPHSTSDECN IGGYKVPQDT IVLINAWAIH RDPELWTEAT TFKPERFEKK 

       430        440        450        460        470        480 
GELEKLIAFG MGRRACPGEG LAIRAISMTL ALLIQCFDWK LINGDKIDLA ERDGFTLTKL 

       490 
VPLKAMCKSR PVINKVFKQ 

« Hide

References

[1]"Two new cytochrome P450 cDNAs from elicitor-induced Licorice (Glycyrrhiza echinata L.) cells."
Akashi T., Aoki T., Kameya N., Nakamura I., Ayabe S.
Plant Gene Register PGR97-167
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase."
Akashi T., Aoki T., Ayabe S.
Biochem. Biophys. Res. Commun. 251:67-70(1998) [PubMed: 9790908] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB001379 mRNA. Translation: BAA22422.1.

3D structure databases

ProteinModelPortalP93147.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
Gene3DG3DSA:1.10.630.10. Cyt_P450. 1 hit.
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC81E1_GLYEC
AccessionPrimary (citable) accession number: P93147
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: September 21, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families