P93147 (C81E1_GLYEC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isoflavone 2'-hydroxylase
EC=1.14.13.89

Alternative name(s):
CYP GE-3
Cytochrome P450 81E1
Cytochrome P450 91A4

Gene names

Name:	CYP81E1
Synonyms:	CYP91A4

Organism

Glycyrrhiza echinata (Licorice)

Taxonomic identifier

46348 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Galegeae › Glycyrrhiza

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the hydroxylation of isoflavones, daidzein and formononetin, to yield 2'-hydroxyisoflavones, 2'-hydroxydaidzein, and 2'-hydroxyformononetin, respectively.
Catalytic activity	An isoflavone + NADPH + O2 = a 2'-hydroxyisoflavone + NADP+ + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Membrane Potential.
Induction	By fungal elicitor.
Miscellaneous	Involved in the biosynthesis of isoflavonoid-derived antimicrobial compounds of legumes.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro isoflavone 2'-hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Isoflavone 2'-hydroxylase		PRO_0000052160
Sites
	Metal binding	436	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93147 [UniParc]. Last modified January 1, 1998. Version 2. Checksum: 4D03AFAC818C04DB	FASTA	499	57,192
	10 20 30 40 50 60 MEILSLLSYS VFYLALFFIF NIVIRARKFK NLPPGPPSLP IIGNLHHLKR PLHRTFKGLS 70 80 90 100 110 120 EKYGHVFSLW FGSRLVVVVS SASEFQQCFT KNDVVLANRP RFLSGKYIFY NYTTLGSTSY 130 140 150 160 170 180 GEHWRNLRRI TALDVLSNHR INSFSGIRRD ETQRLITRLA DDSSTNFAEM ELSSRLYDMT 190 200 210 220 230 240 FNNIMRMISG KRYYGEDCDT SDLQEASQFR DMVSELLQLS GANNKTDFMP LLRFLDFENL 250 260 270 280 290 300 EKRLKDISGK TDAFLRGLIE EHRTKKERAN TMIDHLLNLQ DSQPEYYTDQ IIKGLALAML 310 320 330 340 350 360 LAGTDSSAVT LEWSMSNLLN HPEVLKKVKD ELDTHVGQDR LVDESDLPKL TYLKNVINET 370 380 390 400 410 420 LRLYTPAPLL LPHSTSDECN IGGYKVPQDT IVLINAWAIH RDPELWTEAT TFKPERFEKK 430 440 450 460 470 480 GELEKLIAFG MGRRACPGEG LAIRAISMTL ALLIQCFDWK LINGDKIDLA ERDGFTLTKL 490 VPLKAMCKSR PVINKVFKQ

References

[1]	"Two new cytochrome P450 cDNAs from elicitor-induced Licorice (Glycyrrhiza echinata L.) cells." Akashi T., Aoki T., Kameya N., Nakamura I., Ayabe S. Plant Gene Register PGR97-167 Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"CYP81E1, a cytochrome P450 cDNA of licorice (Glycyrrhiza echinata L.), encodes isoflavone 2'-hydroxylase." Akashi T., Aoki T., Ayabe S. Biochem. Biophys. Res. Commun. 251:67-70(1998) [PubMed: 9790908] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB001379 mRNA. Translation: BAA22422.1.
3D structure databases
ProteinModelPortal	P93147.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C81E1_GLYEC

Accession

Primary (citable) accession number: P93147

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	September 21, 2011
This is version 67 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program