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P93119

- TOP1_DAUCA

UniProt

P93119 - TOP1_DAUCA

Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Daucus carota (Wild carrot)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei412 – 4121Interaction with DNABy similarity
    Sitei444 – 4441Interaction with DNABy similarity
    Sitei502 – 5021Interaction with DNABy similarity
    Sitei528 – 5281Interaction with DNABy similarity
    Sitei570 – 5701Interaction with DNABy similarity
    Sitei632 – 6321Interaction with DNABy similarity
    Sitei650 – 6501Interaction with DNABy similarity
    Active sitei749 – 7491O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase I
    Gene namesi
    Name:TOP1
    OrganismiDaucus carota (Wild carrot)
    Taxonomic identifieri4039 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. chromosome Source: InterPro
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 790790DNA topoisomerase 1PRO_0000145207Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP93119.
    SMRiP93119. Positions 218-635, 734-784.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni426 – 4272Interaction with DNABy similarity
    Regioni490 – 4956Interaction with DNABy similarity
    Regioni581 – 5833Interaction with DNABy similarity

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Family and domain databases

    Gene3Di1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view]
    PfamiPF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SMARTiSM00435. TOPEUc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P93119-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSNPGITVI KQNASNVMEK TLKEEGQSRS NSEDSDDDKP LSHKLSSGAL    50
    NGNSHHIRMG SNLSCPSPYT SPKPRIIKGP EDEMLLSSEL QLNAGASNAE 100
    SSDSDESKPL ASELEDPSSS LNKRPLIQPK NSDPSPSKKA KLSEPSAPTN 150
    RKLKTAEQEE AAADDDPSIS NRNKKSTTPA SKVSDKKKRP DVSASVNKVD 200
    FSKSLKVPLV LVKGRNGQPL VHNGVIFPPL YKPHGVKMLY RENPVDLTPE 250
    QEEVATMFAV MLETEYMTKP KFRENFMSDW RKILGEKHII QNLEDCDFTP 300
    IYEWHQREKE KKKQMSTDEK KAIKEGKDET RKEKYMWAVV DGMSREKVGN 350
    FRVGTTRVVQ RSWRASKDPQ VKKNVYSQID ITINIGKDAP IPEPPIPGER 400
    WKEIRHDNTV TWLAFWNDPI NPKEFKYVFL AASSSLKGKV TREKYEKSRK 450
    LKDYIEGIRA AAYTKDFASK DSKKRQIAVA TYLIDKLALR AGNEKDDDEA 500
    DTVGCCTLKV ENVETKRPNI LKFDFLGKDS IRYQNEVEVE PRVFSAIEQF 550
    RSGKEGGDDL FDQLDTSKLN AHLKELMPGL TAKVFRTYNA SITLDEMVRC 600
    KLLSRETKGG DVAEKVVVYQ HANKEVAIIC NHQRTVSKSH SAQMVRLNEK 650
    IEELKGLLKE LQEDLTRVNK GKPPLKNSDG KPKRNLNLKR YKGKLLKLIQ 700
    KLRKWSETRR LSVTKKIAQT NTKIEKMERD KETKEDLKTV ALGTSKINYL 750
    DPRITVAWCK RHDVPIEKIF NKSLLAKFAW AMDVVLIFRF 790
    Length:790
    Mass (Da):89,771
    Last modified:May 1, 1997 - v1
    Checksum:iCE1D7DA3FF3291F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60440 mRNA. Translation: AAB41401.1.
    PIRiJC5749.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U60440 mRNA. Translation: AAB41401.1 .
    PIRi JC5749.

    3D structure databases

    ProteinModelPortali P93119.
    SMRi P93119. Positions 218-635, 734-784.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.41. 1 hit.
    1.10.132.10. 1 hit.
    2.170.11.10. 2 hits.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR013034. DNA_topo_domain1.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR025834. TopoI_C_dom.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR014727. TopoI_cat_a/b-sub_euk.
    IPR013500. TopoI_cat_euk.
    IPR008336. TopoI_DNA-bd_euk.
    IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
    IPR013499. TopoI_euk.
    [Graphical view ]
    Pfami PF14370. Topo_C_assoc. 1 hit.
    PF01028. Topoisom_I. 1 hit.
    PF02919. Topoisom_I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SMARTi SM00435. TOPEUc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56349. SSF56349. 2 hits.
    SSF56741. SSF56741. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a cDNA encoding DNA topoisomerase I in Daucus carota and expression analysis in relation to cell proliferation."
      Balestrazzi A., Toscano I., Bernacchia G., Luo M., Otte S., Carbonera D.
      Gene 183:183-190(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Lunga di Amsterdam.

    Entry informationi

    Entry nameiTOP1_DAUCA
    AccessioniPrimary (citable) accession number: P93119
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3