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Protein

DNA topoisomerase 1

Gene

TOP1

Organism
Daucus carota (Wild carrot)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity).By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei412 – 4121Interaction with DNABy similarity
Sitei444 – 4441Interaction with DNABy similarity
Sitei502 – 5021Interaction with DNABy similarity
Sitei528 – 5281Interaction with DNABy similarity
Sitei570 – 5701Interaction with DNABy similarity
Sitei632 – 6321Interaction with DNABy similarity
Sitei650 – 6501Interaction with DNABy similarity
Active sitei749 – 7491O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
OrganismiDaucus carota (Wild carrot)
Taxonomic identifieri4039 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790DNA topoisomerase 1PRO_0000145207Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP93119.
SMRiP93119. Positions 218-635, 734-784.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni426 – 4272Interaction with DNABy similarity
Regioni490 – 4956Interaction with DNABy similarity
Regioni581 – 5833Interaction with DNABy similarity

Sequence similaritiesi

Belongs to the type IB topoisomerase family.Curated

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSNPGITVI KQNASNVMEK TLKEEGQSRS NSEDSDDDKP LSHKLSSGAL
60 70 80 90 100
NGNSHHIRMG SNLSCPSPYT SPKPRIIKGP EDEMLLSSEL QLNAGASNAE
110 120 130 140 150
SSDSDESKPL ASELEDPSSS LNKRPLIQPK NSDPSPSKKA KLSEPSAPTN
160 170 180 190 200
RKLKTAEQEE AAADDDPSIS NRNKKSTTPA SKVSDKKKRP DVSASVNKVD
210 220 230 240 250
FSKSLKVPLV LVKGRNGQPL VHNGVIFPPL YKPHGVKMLY RENPVDLTPE
260 270 280 290 300
QEEVATMFAV MLETEYMTKP KFRENFMSDW RKILGEKHII QNLEDCDFTP
310 320 330 340 350
IYEWHQREKE KKKQMSTDEK KAIKEGKDET RKEKYMWAVV DGMSREKVGN
360 370 380 390 400
FRVGTTRVVQ RSWRASKDPQ VKKNVYSQID ITINIGKDAP IPEPPIPGER
410 420 430 440 450
WKEIRHDNTV TWLAFWNDPI NPKEFKYVFL AASSSLKGKV TREKYEKSRK
460 470 480 490 500
LKDYIEGIRA AAYTKDFASK DSKKRQIAVA TYLIDKLALR AGNEKDDDEA
510 520 530 540 550
DTVGCCTLKV ENVETKRPNI LKFDFLGKDS IRYQNEVEVE PRVFSAIEQF
560 570 580 590 600
RSGKEGGDDL FDQLDTSKLN AHLKELMPGL TAKVFRTYNA SITLDEMVRC
610 620 630 640 650
KLLSRETKGG DVAEKVVVYQ HANKEVAIIC NHQRTVSKSH SAQMVRLNEK
660 670 680 690 700
IEELKGLLKE LQEDLTRVNK GKPPLKNSDG KPKRNLNLKR YKGKLLKLIQ
710 720 730 740 750
KLRKWSETRR LSVTKKIAQT NTKIEKMERD KETKEDLKTV ALGTSKINYL
760 770 780 790
DPRITVAWCK RHDVPIEKIF NKSLLAKFAW AMDVVLIFRF
Length:790
Mass (Da):89,771
Last modified:May 1, 1997 - v1
Checksum:iCE1D7DA3FF3291F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60440 mRNA. Translation: AAB41401.1.
PIRiJC5749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U60440 mRNA. Translation: AAB41401.1.
PIRiJC5749.

3D structure databases

ProteinModelPortaliP93119.
SMRiP93119. Positions 218-635, 734-784.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of a cDNA encoding DNA topoisomerase I in Daucus carota and expression analysis in relation to cell proliferation."
    Balestrazzi A., Toscano I., Bernacchia G., Luo M., Otte S., Carbonera D.
    Gene 183:183-190(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Lunga di Amsterdam.

Entry informationi

Entry nameiTOP1_DAUCA
AccessioniPrimary (citable) accession number: P93119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.