SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P93119

- TOP1_DAUCA

UniProt

P93119 - TOP1_DAUCA

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
DNA topoisomerase 1
Gene
TOP1
Organism
Daucus carota (Carrot)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei412 – 4121Interaction with DNA By similarity
Sitei444 – 4441Interaction with DNA By similarity
Sitei502 – 5021Interaction with DNA By similarity
Sitei528 – 5281Interaction with DNA By similarity
Sitei570 – 5701Interaction with DNA By similarity
Sitei632 – 6321Interaction with DNA By similarity
Sitei650 – 6501Interaction with DNA By similarity
Active sitei749 – 7491O-(3'-phospho-DNA)-tyrosine intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-EC
  4. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Gene namesi
Name:TOP1
OrganismiDaucus carota (Carrot)
Taxonomic identifieri4039 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. chromosome Source: InterPro
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790DNA topoisomerase 1
PRO_0000145207Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP93119.
SMRiP93119. Positions 218-635, 734-784.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni426 – 4272Interaction with DNA By similarity
Regioni490 – 4956Interaction with DNA By similarity
Regioni581 – 5833Interaction with DNA By similarity

Sequence similaritiesi

Family and domain databases

Gene3Di1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProiIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamiPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSiPR00416. EUTPISMRASEI.
SMARTiSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMiSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93119-1 [UniParc]FASTAAdd to Basket

« Hide

MKSNPGITVI KQNASNVMEK TLKEEGQSRS NSEDSDDDKP LSHKLSSGAL    50
NGNSHHIRMG SNLSCPSPYT SPKPRIIKGP EDEMLLSSEL QLNAGASNAE 100
SSDSDESKPL ASELEDPSSS LNKRPLIQPK NSDPSPSKKA KLSEPSAPTN 150
RKLKTAEQEE AAADDDPSIS NRNKKSTTPA SKVSDKKKRP DVSASVNKVD 200
FSKSLKVPLV LVKGRNGQPL VHNGVIFPPL YKPHGVKMLY RENPVDLTPE 250
QEEVATMFAV MLETEYMTKP KFRENFMSDW RKILGEKHII QNLEDCDFTP 300
IYEWHQREKE KKKQMSTDEK KAIKEGKDET RKEKYMWAVV DGMSREKVGN 350
FRVGTTRVVQ RSWRASKDPQ VKKNVYSQID ITINIGKDAP IPEPPIPGER 400
WKEIRHDNTV TWLAFWNDPI NPKEFKYVFL AASSSLKGKV TREKYEKSRK 450
LKDYIEGIRA AAYTKDFASK DSKKRQIAVA TYLIDKLALR AGNEKDDDEA 500
DTVGCCTLKV ENVETKRPNI LKFDFLGKDS IRYQNEVEVE PRVFSAIEQF 550
RSGKEGGDDL FDQLDTSKLN AHLKELMPGL TAKVFRTYNA SITLDEMVRC 600
KLLSRETKGG DVAEKVVVYQ HANKEVAIIC NHQRTVSKSH SAQMVRLNEK 650
IEELKGLLKE LQEDLTRVNK GKPPLKNSDG KPKRNLNLKR YKGKLLKLIQ 700
KLRKWSETRR LSVTKKIAQT NTKIEKMERD KETKEDLKTV ALGTSKINYL 750
DPRITVAWCK RHDVPIEKIF NKSLLAKFAW AMDVVLIFRF 790
Length:790
Mass (Da):89,771
Last modified:May 1, 1997 - v1
Checksum:iCE1D7DA3FF3291F5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60440 mRNA. Translation: AAB41401.1.
PIRiJC5749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U60440 mRNA. Translation: AAB41401.1 .
PIRi JC5749.

3D structure databases

ProteinModelPortali P93119.
SMRi P93119. Positions 218-635, 734-784.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProi IPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view ]
Pfami PF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view ]
PRINTSi PR00416. EUTPISMRASEI.
SMARTi SM00435. TOPEUc. 1 hit.
[Graphical view ]
SUPFAMi SSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning of a cDNA encoding DNA topoisomerase I in Daucus carota and expression analysis in relation to cell proliferation."
    Balestrazzi A., Toscano I., Bernacchia G., Luo M., Otte S., Carbonera D.
    Gene 183:183-190(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Lunga di Amsterdam.

Entry informationi

Entry nameiTOP1_DAUCA
AccessioniPrimary (citable) accession number: P93119
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi