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P93119 (TOP1_DAUCA) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 1

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase I
Gene names
Name:TOP1
OrganismDaucus carota (Carrot)
Taxonomic identifier4039 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeScandiceaeDaucinaeDaucus

Protein attributes

Sequence length790 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.

Subcellular location

Nucleus By similarity.

Miscellaneous

Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.

Sequence similarities

Belongs to the type IB topoisomerase family.

Ontologies

Keywords
   Cellular componentNucleus
   LigandDNA-binding
   Molecular functionIsomerase
Topoisomerase
Gene Ontology (GO)
   Biological_processDNA topological change

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchromosome

Inferred from electronic annotation. Source: InterPro

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA topoisomerase type I activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA topoisomerase type II (ATP-hydrolyzing) activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 790790DNA topoisomerase 1
PRO_0000145207

Regions

Region426 – 4272Interaction with DNA By similarity
Region490 – 4956Interaction with DNA By similarity
Region581 – 5833Interaction with DNA By similarity

Sites

Active site7491O-(3'-phospho-DNA)-tyrosine intermediate By similarity
Site4121Interaction with DNA By similarity
Site4441Interaction with DNA By similarity
Site5021Interaction with DNA By similarity
Site5281Interaction with DNA By similarity
Site5701Interaction with DNA By similarity
Site6321Interaction with DNA By similarity
Site6501Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P93119 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: CE1D7DA3FF3291F5

FASTA79089,771
        10         20         30         40         50         60 
MKSNPGITVI KQNASNVMEK TLKEEGQSRS NSEDSDDDKP LSHKLSSGAL NGNSHHIRMG 

        70         80         90        100        110        120 
SNLSCPSPYT SPKPRIIKGP EDEMLLSSEL QLNAGASNAE SSDSDESKPL ASELEDPSSS 

       130        140        150        160        170        180 
LNKRPLIQPK NSDPSPSKKA KLSEPSAPTN RKLKTAEQEE AAADDDPSIS NRNKKSTTPA 

       190        200        210        220        230        240 
SKVSDKKKRP DVSASVNKVD FSKSLKVPLV LVKGRNGQPL VHNGVIFPPL YKPHGVKMLY 

       250        260        270        280        290        300 
RENPVDLTPE QEEVATMFAV MLETEYMTKP KFRENFMSDW RKILGEKHII QNLEDCDFTP 

       310        320        330        340        350        360 
IYEWHQREKE KKKQMSTDEK KAIKEGKDET RKEKYMWAVV DGMSREKVGN FRVGTTRVVQ 

       370        380        390        400        410        420 
RSWRASKDPQ VKKNVYSQID ITINIGKDAP IPEPPIPGER WKEIRHDNTV TWLAFWNDPI 

       430        440        450        460        470        480 
NPKEFKYVFL AASSSLKGKV TREKYEKSRK LKDYIEGIRA AAYTKDFASK DSKKRQIAVA 

       490        500        510        520        530        540 
TYLIDKLALR AGNEKDDDEA DTVGCCTLKV ENVETKRPNI LKFDFLGKDS IRYQNEVEVE 

       550        560        570        580        590        600 
PRVFSAIEQF RSGKEGGDDL FDQLDTSKLN AHLKELMPGL TAKVFRTYNA SITLDEMVRC 

       610        620        630        640        650        660 
KLLSRETKGG DVAEKVVVYQ HANKEVAIIC NHQRTVSKSH SAQMVRLNEK IEELKGLLKE 

       670        680        690        700        710        720 
LQEDLTRVNK GKPPLKNSDG KPKRNLNLKR YKGKLLKLIQ KLRKWSETRR LSVTKKIAQT 

       730        740        750        760        770        780 
NTKIEKMERD KETKEDLKTV ALGTSKINYL DPRITVAWCK RHDVPIEKIF NKSLLAKFAW 

       790 
AMDVVLIFRF 

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References

[1]"Cloning of a cDNA encoding DNA topoisomerase I in Daucus carota and expression analysis in relation to cell proliferation."
Balestrazzi A., Toscano I., Bernacchia G., Luo M., Otte S., Carbonera D.
Gene 183:183-190(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Lunga di Amsterdam.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U60440 mRNA. Translation: AAB41401.1.
PIRJC5749.

3D structure databases

ProteinModelPortalP93119.
SMRP93119. Positions 218-635, 734-784.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.10.10.41. 1 hit.
1.10.132.10. 1 hit.
2.170.11.10. 2 hits.
3.90.15.10. 1 hit.
InterProIPR011010. DNA_brk_join_enz.
IPR013034. DNA_topo_domain1.
IPR001631. TopoI.
IPR018521. TopoI_AS.
IPR025834. TopoI_C_dom.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR014727. TopoI_cat_a/b-sub_euk.
IPR013500. TopoI_cat_euk.
IPR008336. TopoI_DNA-bd_euk.
IPR013030. TopoI_DNA-bd_mixed-a/b_euk.
IPR013499. TopoI_euk.
[Graphical view]
PfamPF14370. Topo_C_assoc. 1 hit.
PF01028. Topoisom_I. 1 hit.
PF02919. Topoisom_I_N. 1 hit.
[Graphical view]
PRINTSPR00416. EUTPISMRASEI.
SMARTSM00435. TOPEUc. 1 hit.
[Graphical view]
SUPFAMSSF56349. SSF56349. 2 hits.
SSF56741. SSF56741. 1 hit.
PROSITEPS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP1_DAUCA
AccessionPrimary (citable) accession number: P93119
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families