P93115 (MGDG_CUCSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Monogalactosyldiacylglycerol synthase, chloroplastic Short name=CsMGD1 EC=2.4.1.46
Organism	Cucumis sativus (Cucumber)
Taxonomic identifier	3659 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Benincaseae › Cucumis

Protein attributes

Sequence length	525 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the synthesis of the major structural component of photosynthetic membranes. Ref.1
Catalytic activity	UDP-alpha-D-galactose + 1,2-diacyl-sn-glycerol = UDP + 3-beta-D-galactosyl-1,2-diacyl-sn-glycerol.
Subcellular location	Plastid › chloroplast membrane Potential.
Induction	By light and cytokinin. Ref.2 Ref.3
Sequence similarities	Belongs to the glycosyltransferase 28 family.

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid
Domain	Transit peptide
Molecular function	Glycosyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glycolipid biosynthetic process Inferred from electronic annotation. Source: InterPro lipid glycosylation Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1,2-diacylglycerol 3-beta-galactosyltransferase activity Inferred from electronic annotation. Source: EC carbohydrate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 103	103	Chloroplast Ref.1
	Chain	104 – 525	422	Monogalactosyldiacylglycerol synthase, chloroplastic		PRO_0000349424
Regions
	Compositional bias	13 – 76	64	Ser-rich

Sequences

	Sequence		Length	Mass (Da)
	P93115 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 74FE586082EC48BA	FASTA	525	57,863
	10 20 30 40 50 60 MRNPSTVVQE NGSVSDFISQ LGYFAFSSRF LNLNSEGCSG SSSHSLYLNG FENYRCVKRP 70 80 90 100 110 120 PRSGASLSLS SRGSSSLRRF VNEFNNVIKF HCHKPPLGFA SLGGVSDETN GIRDDGFGVS 130 140 150 160 170 180 QDGALPLNKI EAENPKRVLI LMSDTGGGHR ASAEAIKAAF NEEFGNNYQV FITDLWTDHT 190 200 210 220 230 240 PWPFNQLPRS YNFLVKHGTL WKMTYYVTAP KVIHQSNFAA TSTFIAREVA KGLMKYRPDI 250 260 270 280 290 300 IISVHPLMQH VPIRILRSKG LLNKIVFTTV VTDLSTCHPT WFHKLVTRCY CPSTEVAKRA 310 320 330 340 350 360 LTAGLQPSKL KVFGLPVRPS FVKPIRPKIE LRKELGMDEN LPAVLLMGGG EGMGPIEATA 370 380 390 400 410 420 KALSKALYDE NHGEPIGQVL VICGHNKKLA GRLRSIDWKV PVQVKGFVTK MEECMGACDC 430 440 450 460 470 480 IITKAGPGTI AEAMIRGLPI ILNDYIAGQE AGNVPYVVEN GCGKFSKSPK EIANIVAKWF 490 500 510 520 GPKADELLIM SQNALRLARP DAVFKIVHDL HELVKQRSFV PQYSG

References

[1]	"Cloning of the gene for monogalactosyldiacylglycerol synthase and its evolutionary origin." Shimojima M., Ohta H., Iwamatsu A., Masuda T., Shioi Y., Takamiya K. Proc. Natl. Acad. Sci. U.S.A. 94:333-337(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, PROTEIN SEQUENCE OF 104-108; 137-148; 197-199; 203-228; 265-275; 285-298; 366-383; 389-397; 406-410 AND 506-525. Strain: cv. Aonagajibai.
[2]	"Transcriptional regulation by light and phytohormones of the MGD gene in cucumber." Yamaryo Y., Kanai D., Awai K., Masuda T., Shimada H., Takamiya K., Ohta H. Biochem. Soc. Trans. 28:738-740(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY LIGHT AND CYTOKININ.
[3]	"Light and cytokinin play a co-operative role in MGDG synthesis in greening cucumber cotyledons." Yamaryo Y., Kanai D., Awai K., Shimojima M., Masuda T., Shimada H., Takamiya K., Ohta H. Plant Cell Physiol. 44:844-855(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION BY LIGHT AND CYTOKININ.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62622 mRNA. Translation: AAC49624.1.
PIR	T10478.
3D structure databases
ProteinModelPortal	P93115.
ModBase	Search...
Protein family/group databases
CAZy	GT28. Glycosyltransferase Family 28.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR009695. Diacylglyc_glucosyltr_N. IPR007235. Glyco_trans_28_C. [Graphical view]
Pfam	PF04101. Glyco_tran_28_C. 1 hit. PF06925. MGDG_synth. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGDG_CUCSA

Accession

Primary (citable) accession number: P93115

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	May 1, 1997
Last modified:	April 3, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program