Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P93111

- HEM11_CUCSA

UniProt

P93111 - HEM11_CUCSA

Protein

Glutamyl-tRNA reductase 1, chloroplastic

Gene

HEMA1

Organism
Cucumis sativus (Cucumber)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 92 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).By similarity

    Catalytic activityi

    L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei151 – 1511NucleophileBy similarity
    Sitei200 – 2001Important for activityBy similarity
    Binding sitei210 – 2101SubstrateBy similarity
    Binding sitei221 – 2211SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi292 – 2976NADPBy similarity

    GO - Molecular functioni

    1. glutamyl-tRNA reductase activity Source: UniProtKB-EC
    2. NADP binding Source: InterPro

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-KW
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00316.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamyl-tRNA reductase 1, chloroplastic (EC:1.2.1.70)
    Short name:
    GluTR
    Gene namesi
    Name:HEMA1
    OrganismiCucumis sativus (Cucumber)
    Taxonomic identifieri3659 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

    Subcellular locationi

    Plastidchloroplast By similarity

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 552Glutamyl-tRNA reductase 1, chloroplasticPRO_0000013309
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Expressioni

    Tissue specificityi

    Primarily in cotyledons and hypocotyls of greening cucumber seedlings.

    Structurei

    3D structure databases

    ProteinModelPortaliP93111.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni150 – 1534Substrate bindingBy similarity
    Regioni215 – 2173Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glutamyl-tRNA reductase family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK02492.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    HAMAPiMF_00087. Glu_tRNA_reductase.
    InterProiIPR000343. 4pyrrol_synth_GluRdtase.
    IPR015896. 4pyrrol_synth_GluRdtase_dimer.
    IPR015895. 4pyrrol_synth_GluRdtase_N.
    IPR018214. GluRdtase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view]
    PfamiPF00745. GlutR_dimer. 1 hit.
    PF05201. GlutR_N. 1 hit.
    PF01488. Shikimate_DH. 1 hit.
    [Graphical view]
    SUPFAMiSSF69075. SSF69075. 1 hit.
    SSF69742. SSF69742. 1 hit.
    TIGRFAMsiTIGR01035. hemA. 1 hit.
    PROSITEiPS00747. GLUTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P93111-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAVSTSFSGA KLEALLFKSA SNSSSTRNLS SSHLPGFCKS IRTRRILFQR    50
    TGVSSFTPFK CELASSDVLV QNDEIDPPKS SNLSALEQLK TSAVDRYTKE 100
    RSSIVVIGLS IHTTPVEMRE KLAIPEAEWP RAIGELCGLN HIEEAAVLST 150
    CNRMEIYVVA LSQHRGVKEV TEWMSKTSGI PVSEICQHRF LLYNNDATQH 200
    IFEVSAGLDS LVLGEGQILA QVKQVVKVGQ GVAGFGRNIS GLFKHAITVG 250
    KRVRTETNIA AGAVSVSSAA VELALMKLPE PSHATARMLV IGAGKMGKLV 300
    IKHLVAKGCT KMVVVNRSEE RVTAIREEMK DVEIIYKPLT EMLSCTAEAD 350
    VIFTSTASES LLFTKEQVKD LPPVGHDVGG LRLFIDISVP RNVGACINNL 400
    EDVRVYNVDD LKEVVAANKE DRLRKAMEAQ SIITEESKQF EAWRDSLETV 450
    PTIKKLRAYA ERIRTAELEK CLSKMGDDIP KKTRRAVDDL SRGIVNKLLH 500
    GPMQHLRCDG SDSRTLSETL ENMHALNRMF SLETEIAVLE QKIRAKVEQN 550
    QK 552
    Length:552
    Mass (Da):60,895
    Last modified:May 1, 1997 - v1
    Checksum:i9C7A72AF24FF00F5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50407 mRNA. Translation: BAA08910.1.
    PIRiT10186.
    RefSeqiNP_001267503.1. NM_001280574.1.

    Genome annotation databases

    GeneIDi101220615.
    KEGGicsv:101220615.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50407 mRNA. Translation: BAA08910.1 .
    PIRi T10186.
    RefSeqi NP_001267503.1. NM_001280574.1.

    3D structure databases

    ProteinModelPortali P93111.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 101220615.
    KEGGi csv:101220615.

    Phylogenomic databases

    KOi K02492.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00316 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    HAMAPi MF_00087. Glu_tRNA_reductase.
    InterProi IPR000343. 4pyrrol_synth_GluRdtase.
    IPR015896. 4pyrrol_synth_GluRdtase_dimer.
    IPR015895. 4pyrrol_synth_GluRdtase_N.
    IPR018214. GluRdtase_CS.
    IPR016040. NAD(P)-bd_dom.
    IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
    [Graphical view ]
    Pfami PF00745. GlutR_dimer. 1 hit.
    PF05201. GlutR_N. 1 hit.
    PF01488. Shikimate_DH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69075. SSF69075. 1 hit.
    SSF69742. SSF69742. 1 hit.
    TIGRFAMsi TIGR01035. hemA. 1 hit.
    PROSITEi PS00747. GLUTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential expression of two hemA mRNAs encoding glutamyl-tRNA reductase proteins in greening cucumber seedlings."
      Tanaka R., Yoshida K., Nakayashiki T., Masuda T., Tsuji H., Inokuchi H., Tanaka A.
      Plant Physiol. 110:1223-1230(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Aonagajibai.
      Tissue: Cotyledon.

    Entry informationi

    Entry nameiHEM11_CUCSA
    AccessioniPrimary (citable) accession number: P93111
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 92 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA By similarity.By similarity

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3