P93110 (ACEA_CUCMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Isocitrate lyase Short name=ICL Short name=Isocitrase Short name=Isocitratase EC=4.1.3.1
Organism	Cucurbita maxima (Pumpkin) (Winter squash)
Taxonomic identifier	3661 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Cucurbiteae › Cucurbita

Protein attributes

Sequence length	576 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in storage lipid mobilization during the growth of higher plant seedling.
Catalytic activity	Isocitrate = succinate + glyoxylate.
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Molecular function	Lyase
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-UniPathway tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	isocitrate lyase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 576	576	Isocitrate lyase		PRO_0000068803
Regions
	Motif	574 – 576	3	Microbody targeting signal Potential
Sites
	Active site	213	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P93110 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 269D93D4F13B5990	FASTA	576	64,359
	10 20 30 40 50 60 MATSFSVPSM IMEEEGRFEA EVAEVQAWWN SERFKLTRRP YTAKDVVSLR GSLRQSYASN 70 80 90 100 110 120 DLAKKLWRTL KTHQANSTAS RTFGALDPVQ VTMMAKHLDS IYVSGWQCSS THTSTNEPGP 130 140 150 160 170 180 DLADYPYDTV PNKVEHLFFA QQYHDRKQRE ARMSMSREER AKTPYVDYLK PIIADGDTGF 190 200 210 220 230 240 GGTTATVKLC KLFVERGAAG VHIEDQSSVT KKCGHMAGKV LVAVSEHINR LVAARLQFDV 250 260 270 280 290 300 MGVETVLVAR TDAVAATLIQ TNVDSRDHQF ILGATNPNLR GKSLAGVLAE AMAAGKTGAE 310 320 330 340 350 360 LQALEDQWIS MAQLKTFSEC VTDAIMNSNG TESEKRRKLD EWMNHSSYEK CISNEQGREI 370 380 390 400 410 420 AEKLGLKNLF WDWDLPRTRE GFYRFKGSVM AAIVRGWAFA PHADLIWMET SSPDLVECTT 430 440 450 460 470 480 FAKGVKSVHP EIMLAYNLSP SFNWDASGMS DKQMEEFIPT IARLGFCWQF ITLAGFHADA 490 500 510 520 530 540 LVIDTFARDY ARRGMLAYVE RIQREERNNG VDTLAHQKWS GANYYDRYLK TVQGGISSTA 550 560 570 AMGKGVTEEQ FKESWTRAGA GNLGEEGSVV VAKSRM

References

[1]	"cDNA cloning and expression of a gene for isocitrate lyase in pumpkin cotyledons." Mano S., Hayashi M., Kondo M., Nishimura M. Plant Cell Physiol. 37:941-948(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D78256 mRNA. Translation: BAA11320.1.
3D structure databases
ProteinModelPortal	P93110.
SMR	P93110. Positions 9-535.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00703; UER00719.
Family and domain databases
Gene3D	3.20.20.60. 2 hits.
InterPro	IPR006254. Isocitrate_lyase. IPR000918. Isocitrate_lyase/Pmutase. IPR018523. Isocitrate_lyase_ph_CS. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. [Graphical view]
PANTHER	PTHR21631:SF3. PTHR21631:SF3. 1 hit.
Pfam	PF00463. ICL. 1 hit. [Graphical view]
PIRSF	PIRSF001362. Isocit_lyase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01346. isocit_lyase. 1 hit.
PROSITE	PS00161. ISOCITRATE_LYASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACEA_CUCMA

Accession

Primary (citable) accession number: P93110

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	May 29, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program