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P93046

- XTH31_ARATH

UniProt

P93046 - XTH31_ARATH

Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 31

Gene

XTH31

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (28 Nov 2003)
      Previous versions | rss
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    Functioni

    Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues By similarity.By similarity

    Catalytic activityi

    Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei114 – 1141NucleophileBy similarity
    Active sitei118 – 1181Proton donorBy similarity

    GO - Molecular functioni

    1. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC
    2. xyloglucan-specific endo-beta-1,4-glucanase activity Source: TAIR

    GO - Biological processi

    1. cellular glucan metabolic process Source: InterPro
    2. cell wall macromolecule catabolic process Source: TAIR

    Keywords - Molecular functioni

    Glycosidase, Hydrolase, Transferase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Enzyme and pathway databases

    BioCyciARA:AT3G44990-MONOMER.

    Protein family/group databases

    CAZyiGH16. Glycoside Hydrolase Family 16.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable xyloglucan endotransglucosylase/hydrolase protein 31 (EC:2.4.1.207)
    Short name:
    At-XTH31
    Short name:
    AtXTR8
    Short name:
    XTH-31
    Gene namesi
    Name:XTH31
    Synonyms:ATXG, XTR8
    Ordered Locus Names:At3g44990
    ORF Names:F14D17.60
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G44990.

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: UniProtKB-SubCell
    2. cell wall Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Apoplast, Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 293273Probable xyloglucan endotransglucosylase/hydrolase protein 31PRO_0000011831Add
    BLAST

    Post-translational modificationi

    Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PaxDbiP93046.
    PRIDEiP93046.

    Expressioni

    Tissue specificityi

    Predominantly expressed in root. Weakly expressed in influorescence stems.2 Publications

    Developmental stagei

    Expressed in germinating seeds from 24 hours after imbibation, and reaches a maximum level at 72 hours. After 96 hours, it then decreases.1 Publication

    Inductioni

    By gibberellins Probable. Not induced by auxin.1 PublicationCurated

    Gene expression databases

    GenevestigatoriP93046.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT3G44990.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP93046.
    SMRiP93046. Positions 18-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG324158.
    HOGENOMiHOG000236368.
    InParanoidiP93046.
    KOiK08235.
    OMAiDPKRDHT.
    PhylomeDBiP93046.

    Family and domain databases

    Gene3Di2.60.120.200. 1 hit.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view]
    PfamiPF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005604. XET. 1 hit.
    SUPFAMiSSF49899. SSF49899. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P93046-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSLIFLAL LVLCPSSGHS QRSPSPGYYP SSRVPTSPFD REFRTLWGSQ    50
    HQRREQDVVT LWLDKSTGSG FKSLRPYRSG YFGASIKLQP GFTAGVDTSL 100
    YLSNNQEHPG DHDEVDIEFL GTTPGKPYSL QTNVFVRGSG DRNVIGREMK 150
    FTLWFDPTQD FHHYAILWNP NQIVFFVDDV PIRTYNRKNE AIFPTRPMWV 200
    YGSIWDASDW ATENGRIKAD YRYQPFVAKY KNFKLAGCTA DSSSSCRPPS 250
    PAPMRNRGLS RQQMAALTWA QRNFLVYNYC HDPKRDHTQT PEC 293
    Length:293
    Mass (Da):33,541
    Last modified:November 28, 2003 - v2
    Checksum:iD999B4C62C5ABCA8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111L → V in CAA63553. 1 PublicationCurated
    Sequence conflicti260 – 2601S → T in CAA63553. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92975 mRNA. Translation: CAA63553.1.
    AL353992 Genomic DNA. Translation: CAB89314.1.
    CP002686 Genomic DNA. Translation: AEE77976.1.
    AY056163 mRNA. Translation: AAL07012.1.
    AY136454 mRNA. Translation: AAM97119.1.
    BT006326 mRNA. Translation: AAP13434.1.
    PIRiT48975.
    RefSeqiNP_190085.1. NM_114368.2.
    UniGeneiAt.20372.

    Genome annotation databases

    EnsemblPlantsiAT3G44990.1; AT3G44990.1; AT3G44990.
    GeneIDi823634.
    KEGGiath:AT3G44990.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92975 mRNA. Translation: CAA63553.1 .
    AL353992 Genomic DNA. Translation: CAB89314.1 .
    CP002686 Genomic DNA. Translation: AEE77976.1 .
    AY056163 mRNA. Translation: AAL07012.1 .
    AY136454 mRNA. Translation: AAM97119.1 .
    BT006326 mRNA. Translation: AAP13434.1 .
    PIRi T48975.
    RefSeqi NP_190085.1. NM_114368.2.
    UniGenei At.20372.

    3D structure databases

    ProteinModelPortali P93046.
    SMRi P93046. Positions 18-293.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT3G44990.1-P.

    Protein family/group databases

    CAZyi GH16. Glycoside Hydrolase Family 16.

    Proteomic databases

    PaxDbi P93046.
    PRIDEi P93046.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G44990.1 ; AT3G44990.1 ; AT3G44990 .
    GeneIDi 823634.
    KEGGi ath:AT3G44990.

    Organism-specific databases

    GeneFarmi 2646. 265.
    TAIRi AT3G44990.

    Phylogenomic databases

    eggNOGi NOG324158.
    HOGENOMi HOG000236368.
    InParanoidi P93046.
    KOi K08235.
    OMAi DPKRDHT.
    PhylomeDBi P93046.

    Enzyme and pathway databases

    BioCyci ARA:AT3G44990-MONOMER.

    Gene expression databases

    Genevestigatori P93046.

    Family and domain databases

    Gene3Di 2.60.120.200. 1 hit.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR013320. ConA-like_subgrp.
    IPR000757. Glyco_hydro_16.
    IPR016455. XET.
    IPR010713. XET_C.
    [Graphical view ]
    Pfami PF00722. Glyco_hydro_16. 1 hit.
    PF06955. XET_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005604. XET. 1 hit.
    SUPFAMi SSF49899. SSF49899. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A new cDNA encoding a xyloglucan endo-transglycosylase-related polypeptide (AtXTR8) preferentially expressed in seedling, root and stem of Arabidopsis thaliana."
      Aubert D., Herzog M.
      Plant Sci. 121:187-196(1996)
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Tissue: Seed.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
      Yokoyama R., Nishitani K.
      Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
      Rose J.K.C., Braam J., Fry S.C., Nishitani K.
      Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NOMENCLATURE.

    Entry informationi

    Entry nameiXTH31_ARATH
    AccessioniPrimary (citable) accession number: P93046
    Secondary accession number(s): Q9LXH6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: November 28, 2003
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3