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P93046

- XTH31_ARATH

UniProt

P93046 - XTH31_ARATH

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Protein

Probable xyloglucan endotransglucosylase/hydrolase protein 31

Gene

XTH31

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues (By similarity).By similarity

Catalytic activityi

Breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei114 – 1141NucleophileBy similarity
Active sitei118 – 1181Proton donorBy similarity

GO - Molecular functioni

  1. xyloglucan:xyloglucosyl transferase activity Source: UniProtKB-EC
  2. xyloglucan-specific endo-beta-1,4-glucanase activity Source: TAIR

GO - Biological processi

  1. cellular glucan metabolic process Source: InterPro
  2. cell wall macromolecule catabolic process Source: TAIR
  3. cell wall organization Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Transferase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT3G44990-MONOMER.

Protein family/group databases

CAZyiGH16. Glycoside Hydrolase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable xyloglucan endotransglucosylase/hydrolase protein 31 (EC:2.4.1.207)
Short name:
At-XTH31
Short name:
AtXTR8
Short name:
XTH-31
Gene namesi
Name:XTH31
Synonyms:ATXG, XTR8
Ordered Locus Names:At3g44990
ORF Names:F14D17.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G44990.

Subcellular locationi

GO - Cellular componenti

  1. apoplast Source: UniProtKB-KW
  2. cell wall Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 293273Probable xyloglucan endotransglucosylase/hydrolase protein 31PRO_0000011831Add
BLAST

Post-translational modificationi

Contains at least one intrachain disulfide bond essential for its enzymatic activity.By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP93046.
PRIDEiP93046.

Expressioni

Tissue specificityi

Predominantly expressed in root. Weakly expressed in influorescence stems.2 Publications

Developmental stagei

Expressed in germinating seeds from 24 hours after imbibation, and reaches a maximum level at 72 hours. After 96 hours, it then decreases.1 Publication

Inductioni

By gibberellins (Probable). Not induced by auxin.1 PublicationCurated

Gene expression databases

GenevestigatoriP93046.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G44990.1-P.

Structurei

3D structure databases

ProteinModelPortaliP93046.
SMRiP93046. Positions 18-293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG324158.
HOGENOMiHOG000236368.
InParanoidiP93046.
KOiK08235.
OMAiDPKRDHT.
PhylomeDBiP93046.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view]
PfamiPF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view]
PIRSFiPIRSF005604. XET. 1 hit.
SUPFAMiSSF49899. SSF49899. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93046-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALSLIFLAL LVLCPSSGHS QRSPSPGYYP SSRVPTSPFD REFRTLWGSQ
60 70 80 90 100
HQRREQDVVT LWLDKSTGSG FKSLRPYRSG YFGASIKLQP GFTAGVDTSL
110 120 130 140 150
YLSNNQEHPG DHDEVDIEFL GTTPGKPYSL QTNVFVRGSG DRNVIGREMK
160 170 180 190 200
FTLWFDPTQD FHHYAILWNP NQIVFFVDDV PIRTYNRKNE AIFPTRPMWV
210 220 230 240 250
YGSIWDASDW ATENGRIKAD YRYQPFVAKY KNFKLAGCTA DSSSSCRPPS
260 270 280 290
PAPMRNRGLS RQQMAALTWA QRNFLVYNYC HDPKRDHTQT PEC
Length:293
Mass (Da):33,541
Last modified:November 28, 2003 - v2
Checksum:iD999B4C62C5ABCA8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111L → V in CAA63553. 1 PublicationCurated
Sequence conflicti260 – 2601S → T in CAA63553. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92975 mRNA. Translation: CAA63553.1.
AL353992 Genomic DNA. Translation: CAB89314.1.
CP002686 Genomic DNA. Translation: AEE77976.1.
AY056163 mRNA. Translation: AAL07012.1.
AY136454 mRNA. Translation: AAM97119.1.
BT006326 mRNA. Translation: AAP13434.1.
PIRiT48975.
RefSeqiNP_190085.1. NM_114368.2.
UniGeneiAt.20372.

Genome annotation databases

EnsemblPlantsiAT3G44990.1; AT3G44990.1; AT3G44990.
GeneIDi823634.
KEGGiath:AT3G44990.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92975 mRNA. Translation: CAA63553.1 .
AL353992 Genomic DNA. Translation: CAB89314.1 .
CP002686 Genomic DNA. Translation: AEE77976.1 .
AY056163 mRNA. Translation: AAL07012.1 .
AY136454 mRNA. Translation: AAM97119.1 .
BT006326 mRNA. Translation: AAP13434.1 .
PIRi T48975.
RefSeqi NP_190085.1. NM_114368.2.
UniGenei At.20372.

3D structure databases

ProteinModelPortali P93046.
SMRi P93046. Positions 18-293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT3G44990.1-P.

Protein family/group databases

CAZyi GH16. Glycoside Hydrolase Family 16.

Proteomic databases

PaxDbi P93046.
PRIDEi P93046.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G44990.1 ; AT3G44990.1 ; AT3G44990 .
GeneIDi 823634.
KEGGi ath:AT3G44990.

Organism-specific databases

GeneFarmi 2646. 265.
TAIRi AT3G44990.

Phylogenomic databases

eggNOGi NOG324158.
HOGENOMi HOG000236368.
InParanoidi P93046.
KOi K08235.
OMAi DPKRDHT.
PhylomeDBi P93046.

Enzyme and pathway databases

BioCyci ARA:AT3G44990-MONOMER.

Gene expression databases

Genevestigatori P93046.

Family and domain databases

Gene3Di 2.60.120.200. 1 hit.
InterProi IPR013320. ConA-like_dom.
IPR000757. Glyco_hydro_16.
IPR016455. XET.
IPR010713. XET_C.
[Graphical view ]
Pfami PF00722. Glyco_hydro_16. 1 hit.
PF06955. XET_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF005604. XET. 1 hit.
SUPFAMi SSF49899. SSF49899. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new cDNA encoding a xyloglucan endo-transglycosylase-related polypeptide (AtXTR8) preferentially expressed in seedling, root and stem of Arabidopsis thaliana."
    Aubert D., Herzog M.
    Plant Sci. 121:187-196(1996)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
    Tissue: Seed.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "A comprehensive expression analysis of all members of a gene family encoding cell-wall enzymes allowed us to predict cis-regulatory regions involved in cell-wall construction in specific organs of Arabidopsis."
    Yokoyama R., Nishitani K.
    Plant Cell Physiol. 42:1025-1033(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  6. "The XTH family of enzymes involved in xyloglucan endotransglucosylation and endohydrolysis: current perspectives and a new unifying nomenclature."
    Rose J.K.C., Braam J., Fry S.C., Nishitani K.
    Plant Cell Physiol. 43:1421-1435(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiXTH31_ARATH
AccessioniPrimary (citable) accession number: P93046
Secondary accession number(s): Q9LXH6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: October 29, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

In contrast to group 1 and group 2 endotransglucosylase/hydrolase proteins, it may not contain the ligase activity, and may catalyze endohydrolysis xyloglucan polymers only.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3