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Protein

Fumarate hydratase 1, mitochondrial

Gene

FUM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).By similarity

Catalytic activityi

(S)-malate = fumarate + H2O.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase 2, chloroplastic (FUM2), Fumarate hydratase 1, mitochondrial (FUM1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • fumarate metabolic process Source: GO_Central
  • malate metabolic process Source: GO_Central
  • pollen tube development Source: TAIR
  • response to oxidative stress Source: TAIR
  • response to salt stress Source: TAIR
  • tricarboxylic acid cycle Source: GO_Central

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BRENDAi4.2.1.2 399
ReactomeiR-ATH-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase 1, mitochondrial (EC:4.2.1.2)
Short name:
Fumarase 1
Gene namesi
Name:FUM1
Ordered Locus Names:At2g47510
ORF Names:T30B22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47510
TAIRilocus:2061966 AT2G47510

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28Mitochondrion1 PublicationAdd BLAST28
ChainiPRO_000001032929 – 492Fumarate hydratase 1, mitochondrialAdd BLAST464

Proteomic databases

PaxDbiP93033
PRIDEiP93033

PTM databases

iPTMnetiP93033
SwissPalmiP93033

Expressioni

Gene expression databases

ExpressionAtlasiP93033 baseline and differential
GenevisibleiP93033 AT

Interactioni

Protein-protein interaction databases

BioGridi4699, 2 interactors
IntActiP93033, 1 interactor
STRINGi3702.AT2G47510.1

Structurei

3D structure databases

ProteinModelPortaliP93033
SMRiP93033
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 160B siteBy similarity4
Regioni167 – 169Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1317 Eukaryota
COG0114 LUCA
HOGENOMiHOG000061736
InParanoidiP93033
KOiK01679
OMAiVSFTDNC
OrthoDBiEOG093607CG
PhylomeDBiP93033

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIYVASRRL SGGTTVTALR YATSLRSYST SFREERDTFG PIQVPSDKLW
60 70 80 90 100
GAQTQRSLQN FEIGGERERM PEPIVRAFGV LKKCAAKVNM EYGLDPTIGK
110 120 130 140 150
AIMQAAQEVA EGKLNDHFPL VVWQTGSGTQ SNMNANEVIA NRAAEILGRK
160 170 180 190 200
RGEKCVHPND HVNRSQSSND TFPTVMHIAA ATEINSRLIP SLKTLHSTLE
210 220 230 240 250
SKSFEFKDIV KIGRTHTQDA TPLTLGQEFG GYATQVKYGL NRVTCTLPRL
260 270 280 290 300
YQLAQGGTAV GTGLNTKKGF DVKIAAAVAE ETNLPFVTAE NKFEALAAHD
310 320 330 340 350
ACVETSGSLN TIATSLMKIA NDIRFLGSGP RCGLGELVLP ENEPGSSIMP
360 370 380 390 400
GKVNPTQCEA LTMVCAQVMG NHVAVTVGGS NGHFELNVFK PVIASALLHS
410 420 430 440 450
VRLIADASAS FEKNCVRGIE ANRERISKLL HESLMLVTSL NPKIGYDNAA
460 470 480 490
AVAKKAHKEG CTLKEAALNL GVLTAEEFDT LVVPEKMIGP SD
Length:492
Mass (Da):53,000
Last modified:December 15, 2003 - v2
Checksum:iDF07D2D393966B3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8 – 9RR → A in AAB39989 (Ref. 1) Curated2
Sequence conflicti197S → R in AAB39989 (Ref. 1) Curated1
Sequence conflicti393 – 394IA → TS in AAB39989 (Ref. 1) Curated2
Sequence conflicti442P → R in AAB39989 (Ref. 1) Curated1
Sequence conflicti446Y → C in AAB39989 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82202 mRNA Translation: AAB39989.1
AC002535 Genomic DNA Translation: AAC62859.1
CP002685 Genomic DNA Translation: AEC10852.1
CP002685 Genomic DNA Translation: AEC10853.1
CP002685 Genomic DNA Translation: ANM62150.1
AF020303 Genomic DNA Translation: AAB71399.1
AY054252 mRNA Translation: AAL06911.1
AY062460 mRNA Translation: AAL32538.1
BT003361 mRNA Translation: AAO29979.1
PIRiT00433
RefSeqiNP_001078075.1, NM_001084606.2
NP_001324328.1, NM_001337266.1
NP_182273.1, NM_130319.4
UniGeneiAt.10398

Genome annotation databases

EnsemblPlantsiAT2G47510.1; AT2G47510.1; AT2G47510
AT2G47510.2; AT2G47510.2; AT2G47510
AT2G47510.3; AT2G47510.3; AT2G47510
GeneIDi819364
GrameneiAT2G47510.1; AT2G47510.1; AT2G47510
AT2G47510.2; AT2G47510.2; AT2G47510
AT2G47510.3; AT2G47510.3; AT2G47510
KEGGiath:AT2G47510

Similar proteinsi

Entry informationi

Entry nameiFUM1_ARATH
AccessioniPrimary (citable) accession number: P93033
Secondary accession number(s): O24649
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: April 25, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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