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Protein

Fumarate hydratase 1, mitochondrial

Gene

FUM1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate = fumarate + H2O.

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase 2, chloroplastic (FUM2), Fumarate hydratase 1, mitochondrial (FUM1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei129SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • fumarate metabolic process Source: GO_Central
  • malate metabolic process Source: GO_Central
  • pollen tube development Source: TAIR
  • protein tetramerization Source: GO_Central
  • response to oxidative stress Source: TAIR
  • response to salt stress Source: TAIR
  • tricarboxylic acid cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

BRENDAi4.2.1.2. 399.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER01007.

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase 1, mitochondrial (EC:4.2.1.2)
Short name:
Fumarase 1
Gene namesi
Name:FUM1
Ordered Locus Names:At2g47510
ORF Names:T30B22.19
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47510.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 28MitochondrionSequence analysisAdd BLAST28
ChainiPRO_000001032929 – 492Fumarate hydratase 1, mitochondrialAdd BLAST464

Proteomic databases

PaxDbiP93033.
PRIDEiP93033.

PTM databases

iPTMnetiP93033.
SwissPalmiP93033.

Expressioni

Gene expression databases

GenevisibleiP93033. AT.

Interactioni

Protein-protein interaction databases

BioGridi4699. 2 interactors.
IntActiP93033. 1 interactor.
STRINGi3702.AT2G47510.1.

Structurei

3D structure databases

ProteinModelPortaliP93033.
SMRiP93033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni157 – 160B siteBy similarity4
Regioni167 – 169Substrate bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1317. Eukaryota.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiP93033.
KOiK01679.
OMAiKANEPRI.
OrthoDBiEOG093607CG.
PhylomeDBiP93033.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P93033-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIYVASRRL SGGTTVTALR YATSLRSYST SFREERDTFG PIQVPSDKLW
60 70 80 90 100
GAQTQRSLQN FEIGGERERM PEPIVRAFGV LKKCAAKVNM EYGLDPTIGK
110 120 130 140 150
AIMQAAQEVA EGKLNDHFPL VVWQTGSGTQ SNMNANEVIA NRAAEILGRK
160 170 180 190 200
RGEKCVHPND HVNRSQSSND TFPTVMHIAA ATEINSRLIP SLKTLHSTLE
210 220 230 240 250
SKSFEFKDIV KIGRTHTQDA TPLTLGQEFG GYATQVKYGL NRVTCTLPRL
260 270 280 290 300
YQLAQGGTAV GTGLNTKKGF DVKIAAAVAE ETNLPFVTAE NKFEALAAHD
310 320 330 340 350
ACVETSGSLN TIATSLMKIA NDIRFLGSGP RCGLGELVLP ENEPGSSIMP
360 370 380 390 400
GKVNPTQCEA LTMVCAQVMG NHVAVTVGGS NGHFELNVFK PVIASALLHS
410 420 430 440 450
VRLIADASAS FEKNCVRGIE ANRERISKLL HESLMLVTSL NPKIGYDNAA
460 470 480 490
AVAKKAHKEG CTLKEAALNL GVLTAEEFDT LVVPEKMIGP SD
Length:492
Mass (Da):53,000
Last modified:December 15, 2003 - v2
Checksum:iDF07D2D393966B3E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8 – 9RR → A in AAB39989 (Ref. 1) Curated2
Sequence conflicti197S → R in AAB39989 (Ref. 1) Curated1
Sequence conflicti393 – 394IA → TS in AAB39989 (Ref. 1) Curated2
Sequence conflicti442P → R in AAB39989 (Ref. 1) Curated1
Sequence conflicti446Y → C in AAB39989 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82202 mRNA. Translation: AAB39989.1.
AC002535 Genomic DNA. Translation: AAC62859.1.
CP002685 Genomic DNA. Translation: AEC10852.1.
CP002685 Genomic DNA. Translation: AEC10853.1.
AF020303 Genomic DNA. Translation: AAB71399.1.
AY054252 mRNA. Translation: AAL06911.1.
AY062460 mRNA. Translation: AAL32538.1.
BT003361 mRNA. Translation: AAO29979.1.
PIRiT00433.
RefSeqiNP_001078075.1. NM_001084606.2.
NP_182273.1. NM_130319.4.
UniGeneiAt.10398.

Genome annotation databases

EnsemblPlantsiAT2G47510.1; AT2G47510.1; AT2G47510.
AT2G47510.2; AT2G47510.2; AT2G47510.
GeneIDi819364.
GrameneiAT2G47510.1; AT2G47510.1; AT2G47510.
AT2G47510.2; AT2G47510.2; AT2G47510.
KEGGiath:AT2G47510.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82202 mRNA. Translation: AAB39989.1.
AC002535 Genomic DNA. Translation: AAC62859.1.
CP002685 Genomic DNA. Translation: AEC10852.1.
CP002685 Genomic DNA. Translation: AEC10853.1.
AF020303 Genomic DNA. Translation: AAB71399.1.
AY054252 mRNA. Translation: AAL06911.1.
AY062460 mRNA. Translation: AAL32538.1.
BT003361 mRNA. Translation: AAO29979.1.
PIRiT00433.
RefSeqiNP_001078075.1. NM_001084606.2.
NP_182273.1. NM_130319.4.
UniGeneiAt.10398.

3D structure databases

ProteinModelPortaliP93033.
SMRiP93033.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4699. 2 interactors.
IntActiP93033. 1 interactor.
STRINGi3702.AT2G47510.1.

PTM databases

iPTMnetiP93033.
SwissPalmiP93033.

Proteomic databases

PaxDbiP93033.
PRIDEiP93033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47510.1; AT2G47510.1; AT2G47510.
AT2G47510.2; AT2G47510.2; AT2G47510.
GeneIDi819364.
GrameneiAT2G47510.1; AT2G47510.1; AT2G47510.
AT2G47510.2; AT2G47510.2; AT2G47510.
KEGGiath:AT2G47510.

Organism-specific databases

TAIRiAT2G47510.

Phylogenomic databases

eggNOGiKOG1317. Eukaryota.
COG0114. LUCA.
HOGENOMiHOG000061736.
InParanoidiP93033.
KOiK01679.
OMAiKANEPRI.
OrthoDBiEOG093607CG.
PhylomeDBiP93033.

Enzyme and pathway databases

UniPathwayiUPA00223; UER01007.
BRENDAi4.2.1.2. 399.
ReactomeiR-ATH-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiP93033.

Gene expression databases

GenevisibleiP93033. AT.

Family and domain databases

Gene3Di1.10.275.10. 1 hit.
HAMAPiMF_00743. FumaraseC. 1 hit.
InterProiIPR005677. Fum_hydII.
IPR024083. Fumarase/histidase_N.
IPR018951. Fumarase_C_C.
IPR020557. Fumarate_lyase_CS.
IPR000362. Fumarate_lyase_fam.
IPR022761. Fumarate_lyase_N.
IPR008948. L-Aspartase-like.
[Graphical view]
PANTHERiPTHR11444. PTHR11444. 1 hit.
PfamiPF10415. FumaraseC_C. 1 hit.
PF00206. Lyase_1. 1 hit.
[Graphical view]
PRINTSiPR00149. FUMRATELYASE.
SUPFAMiSSF48557. SSF48557. 1 hit.
TIGRFAMsiTIGR00979. fumC_II. 1 hit.
PROSITEiPS00163. FUMARATE_LYASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUM1_ARATH
AccessioniPrimary (citable) accession number: P93033
Secondary accession number(s): O24649
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: December 15, 2003
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.