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Protein

Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial

Gene

IDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Performs an essential role in the oxidative function of the citric acid cycle.1 Publication

Catalytic activityi

Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei116 – 1161SubstrateBy similarity
Binding sitei147 – 1471SubstrateBy similarity
Sitei154 – 1541Critical for catalysisBy similarity
Sitei201 – 2011Critical for catalysisBy similarity
Metal bindingi234 – 2341Magnesium or manganeseBy similarity
Binding sitei234 – 2341SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • tricarboxylic acid cycle Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD

Enzyme and pathway databases

BioCyciARA:GQT-1494-MONOMER.
MetaCyc:AT2G17130-MONOMER.
BRENDAi1.1.1.41. 399.
ReactomeiREACT_289406. Citric acid cycle (TCA cycle).

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial (EC:1.1.1.41)
Alternative name(s):
IDH-II
Isocitric dehydrogenase 2
NAD(+)-specific ICDH 2
Gene namesi
Name:IDH2
Ordered Locus Names:At2g17130
ORF Names:F6P23.14
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G17130.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
BLAST
Chaini26 – 367342Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrialPRO_0000271288Add
BLAST

Proteomic databases

PaxDbiP93032.
PRIDEiP93032.

Expressioni

Tissue specificityi

Ubiquitous. Predominantly expressed in roots, stems and leaves.2 Publications

Gene expression databases

ExpressionAtlasiP93032. baseline and differential.

Interactioni

Subunit structurei

Heterooligomer of catalytic and regulatory subunits.

Protein-protein interaction databases

BioGridi1575. 1 interaction.
STRINGi3702.AT2G17130.1.

Structurei

3D structure databases

ProteinModelPortaliP93032.
SMRiP93032. Positions 40-366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
InParanoidiP93032.
KOiK00030.
OMAiPSIAYNE.
PhylomeDBiP93032.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P93032-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRQSFSLLK NLRSIASGSK IQTRSVTYMP RPGDGKPRPV TLIPGDGVGP
60 70 80 90 100
LVTNAVQQVM EAMHAPVYFE PFEVHGDMKS LPEGLLESIK KNKVCLKGGL
110 120 130 140 150
KTPVGGGVSS LNVNLRKELD LFASLVNCFN LPGLASRHEN VDIVVIRENT
160 170 180 190 200
EGEYAGLEHE VVPGVVESLK VITKFCSERI AKYAFEYAYL NNRKKVTAVH
210 220 230 240 250
KANIMKLADG LFLESCQEVA KKYPSIAYNE IIVDNCCMQL VARPEQFDVM
260 270 280 290 300
VTPNLYGNLV ANTAAGIAGG TGVMPGGNVG AEYAVFEQGA SAGNVGKDTT
310 320 330 340 350
EEQKNANPVA LLLSSAMMLR HLQFPSFADR LETAVKRVIA EGNCRTEDLG
360
GNSTTQEVVD AVIANLD
Length:367
Mass (Da):39,590
Last modified:January 9, 2007 - v2
Checksum:iB5BC1C422AF757F7
GO
Isoform 2 (identifier: P93032-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-174: Missing.

Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.
Show »
Length:363
Mass (Da):39,149
Checksum:i1F691FD0BE2E7146
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61F → S in AAC49965 (PubMed:9526501).Curated
Sequence conflicti18 – 181G → R in AAC49965 (PubMed:9526501).Curated
Sequence conflicti271 – 2711T → S in AAC49965 (PubMed:9526501).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei171 – 1744Missing in isoform 2. CuratedVSP_027467

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81994 mRNA. Translation: AAC49965.1.
CP002685 Genomic DNA. Translation: AEC06588.1.
CP002685 Genomic DNA. Translation: AEC06589.1.
AK228337 mRNA. Translation: BAF00277.1.
PIRiD84548.
RefSeqiNP_179304.1. NM_127267.2. [P93032-1]
NP_849963.1. NM_179632.1. [P93032-2]
UniGeneiAt.12294.
At.48484.

Genome annotation databases

EnsemblPlantsiAT2G17130.1; AT2G17130.1; AT2G17130. [P93032-1]
GeneIDi816218.
KEGGiath:AT2G17130.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U81994 mRNA. Translation: AAC49965.1.
CP002685 Genomic DNA. Translation: AEC06588.1.
CP002685 Genomic DNA. Translation: AEC06589.1.
AK228337 mRNA. Translation: BAF00277.1.
PIRiD84548.
RefSeqiNP_179304.1. NM_127267.2. [P93032-1]
NP_849963.1. NM_179632.1. [P93032-2]
UniGeneiAt.12294.
At.48484.

3D structure databases

ProteinModelPortaliP93032.
SMRiP93032. Positions 40-366.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi1575. 1 interaction.
STRINGi3702.AT2G17130.1.

Proteomic databases

PaxDbiP93032.
PRIDEiP93032.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G17130.1; AT2G17130.1; AT2G17130. [P93032-1]
GeneIDi816218.
KEGGiath:AT2G17130.

Organism-specific databases

GeneFarmi4366. 439.
TAIRiAT2G17130.

Phylogenomic databases

eggNOGiCOG0473.
HOGENOMiHOG000021113.
InParanoidiP93032.
KOiK00030.
OMAiPSIAYNE.
PhylomeDBiP93032.

Enzyme and pathway databases

BioCyciARA:GQT-1494-MONOMER.
MetaCyc:AT2G17130-MONOMER.
BRENDAi1.1.1.41. 399.
ReactomeiREACT_289406. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiP93032.

Gene expression databases

ExpressionAtlasiP93032. baseline and differential.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR001804. Isocitrate/isopropylmalate_DH.
IPR004434. Isocitrate_DH_NAD.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11835. PTHR11835. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana. Characterization of two closely related subunits."
    Behal R.H., Oliver D.J.
    Plant Mol. Biol. 36:691-698(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  6. "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana."
    Lin M., Behal R.H., Oliver D.J.
    Plant Sci. 166:983-988(2004)
    [AGRICOLA] [Europe PMC]
    Cited for: GENE FAMILY, FUNCTION, TISSUE SPECIFICITY.
  7. "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs."
    Lemaitre T., Hodges M.
    Plant Cell Physiol. 47:634-643(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiIDH2_ARATH
AccessioniPrimary (citable) accession number: P93032
Secondary accession number(s): Q7XJT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2007
Last sequence update: January 9, 2007
Last modified: July 22, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.