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P93032

- IDH2_ARATH

UniProt

P93032 - IDH2_ARATH

Protein

Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial

Gene

IDH2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (09 Jan 2007)
      Previous versions | rss
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    Functioni

    Performs an essential role in the oxidative function of the citric acid cycle.1 Publication

    Catalytic activityi

    Isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH.

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei116 – 1161SubstrateBy similarity
    Binding sitei147 – 1471SubstrateBy similarity
    Sitei154 – 1541Critical for catalysisBy similarity
    Sitei201 – 2011Critical for catalysisBy similarity
    Metal bindingi234 – 2341Magnesium or manganeseBy similarity
    Binding sitei234 – 2341SubstrateBy similarity

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NAD+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. tricarboxylic acid cycle Source: TAIR

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD

    Enzyme and pathway databases

    BioCyciARA:GQT-1494-MONOMER.
    MetaCyc:AT2G17130-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial (EC:1.1.1.41)
    Alternative name(s):
    IDH-II
    Isocitric dehydrogenase 2
    NAD(+)-specific ICDH 2
    Gene namesi
    Name:IDH2
    Ordered Locus Names:At2g17130
    ORF Names:F6P23.14
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G17130.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 2525MitochondrionSequence AnalysisAdd
    BLAST
    Chaini26 – 367342Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrialPRO_0000271288Add
    BLAST

    Proteomic databases

    PaxDbiP93032.
    PRIDEiP93032.

    Expressioni

    Tissue specificityi

    Ubiquitous. Predominantly expressed in roots, stems and leaves.2 Publications

    Gene expression databases

    ArrayExpressiP93032.
    GenevestigatoriP93032.

    Interactioni

    Subunit structurei

    Heterooligomer of catalytic and regulatory subunits.

    Protein-protein interaction databases

    BioGridi1575. 1 interaction.
    STRINGi3702.AT2G17130.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP93032.
    SMRiP93032. Positions 40-366.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0473.
    HOGENOMiHOG000021113.
    InParanoidiP93032.
    KOiK00030.
    OMAiPSIAYNE.
    PhylomeDBiP93032.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11835. PTHR11835. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00175. mito_nad_idh. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P93032-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSRQSFSLLK NLRSIASGSK IQTRSVTYMP RPGDGKPRPV TLIPGDGVGP    50
    LVTNAVQQVM EAMHAPVYFE PFEVHGDMKS LPEGLLESIK KNKVCLKGGL 100
    KTPVGGGVSS LNVNLRKELD LFASLVNCFN LPGLASRHEN VDIVVIRENT 150
    EGEYAGLEHE VVPGVVESLK VITKFCSERI AKYAFEYAYL NNRKKVTAVH 200
    KANIMKLADG LFLESCQEVA KKYPSIAYNE IIVDNCCMQL VARPEQFDVM 250
    VTPNLYGNLV ANTAAGIAGG TGVMPGGNVG AEYAVFEQGA SAGNVGKDTT 300
    EEQKNANPVA LLLSSAMMLR HLQFPSFADR LETAVKRVIA EGNCRTEDLG 350
    GNSTTQEVVD AVIANLD 367
    Length:367
    Mass (Da):39,590
    Last modified:January 9, 2007 - v2
    Checksum:iB5BC1C422AF757F7
    GO
    Isoform 2 (identifier: P93032-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         171-174: Missing.

    Note: Derived from EST data. May be due to a competing acceptor splice site. No experimental confirmation available.

    Show »
    Length:363
    Mass (Da):39,149
    Checksum:i1F691FD0BE2E7146
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61F → S in AAC49965. (PubMed:9526501)Curated
    Sequence conflicti18 – 181G → R in AAC49965. (PubMed:9526501)Curated
    Sequence conflicti271 – 2711T → S in AAC49965. (PubMed:9526501)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei171 – 1744Missing in isoform 2. CuratedVSP_027467

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81994 mRNA. Translation: AAC49965.1.
    CP002685 Genomic DNA. Translation: AEC06588.1.
    CP002685 Genomic DNA. Translation: AEC06589.1.
    AK228337 mRNA. Translation: BAF00277.1.
    PIRiD84548.
    RefSeqiNP_179304.1. NM_127267.2. [P93032-1]
    NP_849963.1. NM_179632.1. [P93032-2]
    UniGeneiAt.12294.
    At.48484.

    Genome annotation databases

    EnsemblPlantsiAT2G17130.1; AT2G17130.1; AT2G17130. [P93032-1]
    GeneIDi816218.
    KEGGiath:AT2G17130.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U81994 mRNA. Translation: AAC49965.1 .
    CP002685 Genomic DNA. Translation: AEC06588.1 .
    CP002685 Genomic DNA. Translation: AEC06589.1 .
    AK228337 mRNA. Translation: BAF00277.1 .
    PIRi D84548.
    RefSeqi NP_179304.1. NM_127267.2. [P93032-1 ]
    NP_849963.1. NM_179632.1. [P93032-2 ]
    UniGenei At.12294.
    At.48484.

    3D structure databases

    ProteinModelPortali P93032.
    SMRi P93032. Positions 40-366.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 1575. 1 interaction.
    STRINGi 3702.AT2G17130.1-P.

    Proteomic databases

    PaxDbi P93032.
    PRIDEi P93032.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G17130.1 ; AT2G17130.1 ; AT2G17130 . [P93032-1 ]
    GeneIDi 816218.
    KEGGi ath:AT2G17130.

    Organism-specific databases

    GeneFarmi 4366. 439.
    TAIRi AT2G17130.

    Phylogenomic databases

    eggNOGi COG0473.
    HOGENOMi HOG000021113.
    InParanoidi P93032.
    KOi K00030.
    OMAi PSIAYNE.
    PhylomeDBi P93032.

    Enzyme and pathway databases

    BioCyci ARA:GQT-1494-MONOMER.
    MetaCyc:AT2G17130-MONOMER.

    Gene expression databases

    ArrayExpressi P93032.
    Genevestigatori P93032.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR001804. Isocitrate/isopropylmalate_DH.
    IPR004434. Isocitrate_DH_NAD.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11835. PTHR11835. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00175. mito_nad_idh. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana. Characterization of two closely related subunits."
      Behal R.H., Oliver D.J.
      Plant Mol. Biol. 36:691-698(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    6. "Characterization of a mutation in the IDH-II subunit of the NAD(+)-dependent isocitrate dehydrogenase from Arabidopsis thaliana."
      Lin M., Behal R.H., Oliver D.J.
      Plant Sci. 166:983-988(2004)
      [AGRICOLA] [Europe PMC]
      Cited for: GENE FAMILY, FUNCTION, TISSUE SPECIFICITY.
    7. "Expression analysis of Arabidopsis thaliana NAD-dependent isocitrate dehydrogenase genes shows the presence of a functional subunit that is mainly expressed in the pollen and absent from vegetative organs."
      Lemaitre T., Hodges M.
      Plant Cell Physiol. 47:634-643(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiIDH2_ARATH
    AccessioniPrimary (citable) accession number: P93032
    Secondary accession number(s): Q7XJT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 9, 2007
    Last sequence update: January 9, 2007
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3