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Protein

Ubiquitin-activating enzyme E1 1

Gene

UBA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.2 Publications

Miscellaneous

Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.1 Publication
Mutation in UBA1 (mos5) suppresses snc1-mediated constitutive resistance and affects the resistance responses conferred by only a subset of R-proteins.1 Publication

Catalytic activityi

ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei502ATP; via amide nitrogenBy similarity1
Binding sitei528ATPBy similarity1
Binding sitei539ATPBy similarity1
Binding sitei552ATPBy similarity1
Active sitei656Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi600 – 601ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: GO_Central
  • protein ubiquitination Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to other organism Source: TAIR

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.B9 399
ReactomeiR-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-activating enzyme E1 1 (EC:6.2.1.451 Publication)
Short name:
AtUBA11 Publication
Alternative name(s):
Protein MODIFIER OF SNC1 5
Gene namesi
Name:UBA1
Synonyms:MOS5
Ordered Locus Names:At2g30110
ORF Names:T27E13.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30110
TAIRilocus:2060854 AT2G30110

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1032 – 1037RMDKKV → S in mos5; enhanced disease susceptibility. 1 Publication6

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003993951 – 1080Ubiquitin-activating enzyme E1 1Add BLAST1080

Proteomic databases

PaxDbiP93028
PRIDEiP93028

PTM databases

iPTMnetiP93028

Expressioni

Tissue specificityi

Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication

Developmental stagei

Expressed over the entire range of development.1 Publication

Gene expression databases

ExpressionAtlasiP93028 baseline and differential
GenevisibleiP93028 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi2911, 2 interactors
IntActiP93028, 1 interactor
STRINGi3702.AT2G30110.1

Structurei

3D structure databases

ProteinModelPortaliP93028
SMRiP93028
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2012 Eukaryota
COG0476 LUCA
HOGENOMiHOG000167329
InParanoidiP93028
KOiK03178
OMAiLKNWSMM
OrthoDBiEOG0936017S
PhylomeDBiP93028

Family and domain databases

Gene3Di3.10.290.60, 1 hit
InterProiView protein in InterPro
IPR032420 E1_4HB
IPR032418 E1_FCCH
IPR000594 ThiF_NAD_FAD-bd
IPR018965 Ub-activating_enz_E1_C
IPR038252 UBA_E1_C_sf
IPR019572 UBA_E1_Cys
IPR035985 Ubiquitin-activating_enz
IPR018075 UBQ-activ_enz_E1
IPR018074 UBQ-activ_enz_E1_CS
IPR033127 UBQ-activ_enz_E1_Cys_AS
IPR000011 UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191 E1_4HB, 1 hit
PF16190 E1_FCCH, 1 hit
PF09358 E1_UFD, 1 hit
PF00899 ThiF, 2 hits
PF10585 UBA_e1_thiolCys, 1 hit
PRINTSiPR01849 UBIQUITINACT
SMARTiView protein in SMART
SM00985 UBA_e1_C, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01408 Ube1, 1 hit
PROSITEiView protein in PROSITE
PS00536 UBIQUITIN_ACTIVAT_1, 1 hit
PS00865 UBIQUITIN_ACTIVAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P93028-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR
60 70 80 90 100
GFHGDSVVQQ IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI
110 120 130 140 150
SGMHGLGAEI AKNLILAGVK SVTLHDERVV ELWDLSSNFV FSEDDVGKNR
160 170 180 190 200
ADASVQKLQD LNNAVVVSSL TKSLNKEDLS GFQVVVFSDI SMERAIEFDD
210 220 230 240 250
YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE PHTGIIASIS
260 270 280 290 300
NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF
310 320 330 340 350
TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF
360 370 380 390 400
DRPPLLHLAF QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK
410 420 430 440 450
VENVDQKLLR HFSFGAKAVL NPMAAMFGGI VGQEVVKACS GKFHPLFQFF
460 470 480 490 500
YFDSVESLPS EPVDSSDFAP RNSRYDAQIS VFGAKFQKKL EDAKVFTVGS
510 520 530 540 550
GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ FLFRDWNIGQ
560 570 580 590 600
AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD
610 620 630 640 650
NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE
660 670 680 690 700
KQAPMCTVHS FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS
710 720 730 740 750
MMSAGDAQAR DTLERIVECL EKEKCETFQD CLTWARLRFE DYFVNRVKQL
760 770 780 790 800
IYTFPEDAAT STGAPFWSAP KRFPRPLQYS SSDPSLLNFI TATAILRAET
810 820 830 840 850
FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK ATTLTTASVD
860 870 880 890 900
DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR
910 920 930 940 950
ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV
960 970 980 990 1000
EAYRNTFANL ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV
1010 1020 1030 1040 1050
LQWLEDKGLS AYSISCGSCL LFNSMFTRHK ERMDKKVVDL ARDVAKVELP
1060 1070 1080
PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR
Length:1,080
Mass (Da):120,251
Last modified:May 1, 1997 - v1
Checksum:i4B442A35DAFD34D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti883D → G in AAP21171 (PubMed:14593172).Curated1
Sequence conflicti883D → G in AAL90910 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80808 Genomic DNA Translation: AAB39246.1
AC004165 Genomic DNA Translation: AAC16961.1
CP002685 Genomic DNA Translation: AEC08346.1
AY090248 mRNA Translation: AAL90910.1
BT006363 mRNA Translation: AAP21171.1
PIRiT00587
RefSeqiNP_565693.1, NM_128566.4
UniGeneiAt.21347

Genome annotation databases

EnsemblPlantsiAT2G30110.1; AT2G30110.1; AT2G30110
GeneIDi817562
GrameneiAT2G30110.1; AT2G30110.1; AT2G30110
KEGGiath:AT2G30110

Similar proteinsi

Entry informationi

Entry nameiUBE11_ARATH
AccessioniPrimary (citable) accession number: P93028
Secondary accession number(s): Q8RX82
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health