P93028 (UBE11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-activating enzyme E1 1
Short name=AtUBA1

Alternative name(s):
Protein MODIFIER OF SNC1 5

Gene names

Name:	UBA1
Synonyms:	MOS5
Ordered Locus Names:	At2g30110
ORF Names:	T27E13.15

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1080 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Ref.1 Ref.5
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Monomer By similarity.
Tissue specificity	Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls. Ref.1
Developmental stage	Expressed over the entire range of development. Ref.1
Miscellaneous	Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency. Mutation in UBA1 (mos5) suppresses snc1-mediated constitutive resistance and affects the resistance responses conferred by only a subset of R-proteins.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to cadmium ion Inferred from expression pattern PubMed 16502469. Source: TAIR response to other organism Inferred from mutant phenotype Ref.5. Source: TAIR
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW small protein activating enzyme activity Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1080	1080	Ubiquitin-activating enzyme E1 1		PRO_0000399395
Regions
	Nucleotide binding	502 – 531	30	ATP By similarity
Sites
	Active site	656	1	Glycyl thioester intermediate By similarity
Experimental info
	Mutagenesis	1032 – 1037	6	RMDKKV → S in mos5; enhanced disease susceptibility. Ref.5
	Sequence conflict	883	1	D → G in AAP21171. Ref.4
	Sequence conflict	883	1	D → G in AAL90910. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P93028 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 4B442A35DAFD34D0	FASTA	1,080	120,251
	10 20 30 40 50 60 MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR GFHGDSVVQQ 70 80 90 100 110 120 IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI SGMHGLGAEI AKNLILAGVK 130 140 150 160 170 180 SVTLHDERVV ELWDLSSNFV FSEDDVGKNR ADASVQKLQD LNNAVVVSSL TKSLNKEDLS 190 200 210 220 230 240 GFQVVVFSDI SMERAIEFDD YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE 250 260 270 280 290 300 PHTGIIASIS NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF 310 320 330 340 350 360 TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF DRPPLLHLAF 370 380 390 400 410 420 QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK VENVDQKLLR HFSFGAKAVL 430 440 450 460 470 480 NPMAAMFGGI VGQEVVKACS GKFHPLFQFF YFDSVESLPS EPVDSSDFAP RNSRYDAQIS 490 500 510 520 530 540 VFGAKFQKKL EDAKVFTVGS GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ 550 560 570 580 590 600 FLFRDWNIGQ AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD 610 620 630 640 650 660 NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE KQAPMCTVHS 670 680 690 700 710 720 FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS MMSAGDAQAR DTLERIVECL 730 740 750 760 770 780 EKEKCETFQD CLTWARLRFE DYFVNRVKQL IYTFPEDAAT STGAPFWSAP KRFPRPLQYS 790 800 810 820 830 840 SSDPSLLNFI TATAILRAET FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK 850 860 870 880 890 900 ATTLTTASVD DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR 910 920 930 940 950 960 ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV EAYRNTFANL 970 980 990 1000 1010 1020 ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV LQWLEDKGLS AYSISCGSCL 1030 1040 1050 1060 1070 1080 LFNSMFTRHK ERMDKKVVDL ARDVAKVELP PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana." Hatfield P.M., Gosink M.M., Carpenter T.B., Vierstra R.D. Plant J. 11:213-226(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The ubiquitin pathway is required for innate immunity in Arabidopsis." Goritschnig S., Zhang Y., Li X. Plant J. 49:540-551(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF 1032-ARG--VAL-1037.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U80808 Genomic DNA. Translation: AAB39246.1. AC004165 Genomic DNA. Translation: AAC16961.1. CP002685 Genomic DNA. Translation: AEC08346.1. AY090248 mRNA. Translation: AAL90910.1. BT006363 mRNA. Translation: AAP21171.1.
IPI	IPI00545792.
PIR	T00587.
RefSeq	NP_565693.1. NM_128566.3.
UniGene	At.21347.
3D structure databases
HSSP	HSSP built from PDB template 1JW9 based on UniProtKB P12282.
ProteinModelPortal	P93028.
SMR	P93028. Positions 78-336, 460-959.
ModBase	Search...
Protein-protein interaction databases
IntAct	P93028. 1 interaction.
STRING	3702.AT2G30110.1-P.
Proteomic databases
PaxDb	P93028.
PRIDE	P93028.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30110.1; AT2G30110.1; AT2G30110.
GeneID	817562.
KEGG	ath:AT2G30110.
Organism-specific databases
TAIR	At2g30110.
Phylogenomic databases
eggNOG	COG0476.
HOGENOM	HOG000167329.
InParanoid	P93028.
KO	K03178.
OMA	IFNEDFW.
PhylomeDB	P93028.
ProtClustDB	CLSN2688565.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P93028.
Genevestigator	P93028.
Family and domain databases
Gene3D	1.10.3240.10. 1 hit. 3.40.50.720. 4 hits.
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. IPR018965. Ub-activating_enz_e1_C. IPR023280. Ub-like_act_enz_cat_cys_dom. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR018074. UBQ-activ_enz_E1_AS. IPR000011. UBQ/SUMO-activ_enz_E1-like. [Graphical view]
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
SMART	SM00985. UBA_e1_C. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 2 hits.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. 1 hit. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE11_ARATH

Accession

Primary (citable) accession number: P93028
Secondary accession number(s): Q8RX82

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program