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P93028

- UBE11_ARATH

UniProt

P93028 - UBE11_ARATH

Protein

Ubiquitin-activating enzyme E1 1

Gene

UBA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.2 Publications

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei656 – 6561Glycyl thioester intermediatePROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi502 – 53130ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ligase activity Source: UniProtKB-KW
    3. small protein activating enzyme activity Source: InterPro
    4. ubiquitin-protein transferase activity Source: TAIR

    GO - Biological processi

    1. protein ubiquitination Source: TAIR
    2. response to cadmium ion Source: TAIR
    3. response to other organism Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-activating enzyme E1 1
    Short name:
    AtUBA1
    Alternative name(s):
    Protein MODIFIER OF SNC1 5
    Gene namesi
    Name:UBA1
    Synonyms:MOS5
    Ordered Locus Names:At2g30110
    ORF Names:T27E13.15
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G30110.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. plasma membrane Source: TAIR
    3. plasmodesma Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi1032 – 10376RMDKKV → S in mos5; enhanced disease susceptibility. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10801080Ubiquitin-activating enzyme E1 1PRO_0000399395Add
    BLAST

    Proteomic databases

    PaxDbiP93028.
    PRIDEiP93028.

    Expressioni

    Tissue specificityi

    Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication

    Developmental stagei

    Expressed over the entire range of development.1 Publication

    Gene expression databases

    ArrayExpressiP93028.
    GenevestigatoriP93028.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi2911. 2 interactions.
    IntActiP93028. 1 interaction.
    STRINGi3702.AT2G30110.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP93028.
    SMRiP93028. Positions 73-1077.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-activating E1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0476.
    HOGENOMiHOG000167329.
    InParanoidiP93028.
    KOiK03178.
    OMAiHISKKPV.
    PhylomeDBiP93028.

    Family and domain databases

    Gene3Di1.10.3240.10. 1 hit.
    3.40.50.720. 4 hits.
    InterProiIPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    IPR018965. Ub-activating_enz_e1_C.
    IPR023280. Ub-like_act_enz_cat_cys_dom.
    IPR000127. UBact_repeat.
    IPR019572. Ubiquitin-activating_enzyme.
    IPR018075. UBQ-activ_enz_E1.
    IPR018074. UBQ-activ_enz_E1_AS.
    IPR000011. UBQ/SUMO-activ_enz_E1-like.
    [Graphical view]
    PfamiPF00899. ThiF. 2 hits.
    PF09358. UBA_e1_C. 1 hit.
    PF10585. UBA_e1_thiolCys. 1 hit.
    PF02134. UBACT. 2 hits.
    [Graphical view]
    PRINTSiPR01849. UBIQUITINACT.
    SMARTiSM00985. UBA_e1_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF69572. SSF69572. 2 hits.
    TIGRFAMsiTIGR01408. Ube1. 1 hit.
    PROSITEiPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
    PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P93028-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR     50
    GFHGDSVVQQ IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI 100
    SGMHGLGAEI AKNLILAGVK SVTLHDERVV ELWDLSSNFV FSEDDVGKNR 150
    ADASVQKLQD LNNAVVVSSL TKSLNKEDLS GFQVVVFSDI SMERAIEFDD 200
    YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE PHTGIIASIS 250
    NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF 300
    TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF 350
    DRPPLLHLAF QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK 400
    VENVDQKLLR HFSFGAKAVL NPMAAMFGGI VGQEVVKACS GKFHPLFQFF 450
    YFDSVESLPS EPVDSSDFAP RNSRYDAQIS VFGAKFQKKL EDAKVFTVGS 500
    GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ FLFRDWNIGQ 550
    AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD 600
    NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE 650
    KQAPMCTVHS FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS 700
    MMSAGDAQAR DTLERIVECL EKEKCETFQD CLTWARLRFE DYFVNRVKQL 750
    IYTFPEDAAT STGAPFWSAP KRFPRPLQYS SSDPSLLNFI TATAILRAET 800
    FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK ATTLTTASVD 850
    DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR 900
    ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV 950
    EAYRNTFANL ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV 1000
    LQWLEDKGLS AYSISCGSCL LFNSMFTRHK ERMDKKVVDL ARDVAKVELP 1050
    PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR 1080
    Length:1,080
    Mass (Da):120,251
    Last modified:May 1, 1997 - v1
    Checksum:i4B442A35DAFD34D0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti883 – 8831D → G in AAP21171. (PubMed:14593172)Curated
    Sequence conflicti883 – 8831D → G in AAL90910. (PubMed:14593172)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80808 Genomic DNA. Translation: AAB39246.1.
    AC004165 Genomic DNA. Translation: AAC16961.1.
    CP002685 Genomic DNA. Translation: AEC08346.1.
    AY090248 mRNA. Translation: AAL90910.1.
    BT006363 mRNA. Translation: AAP21171.1.
    PIRiT00587.
    RefSeqiNP_565693.1. NM_128566.3.
    UniGeneiAt.21347.

    Genome annotation databases

    EnsemblPlantsiAT2G30110.1; AT2G30110.1; AT2G30110.
    GeneIDi817562.
    KEGGiath:AT2G30110.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U80808 Genomic DNA. Translation: AAB39246.1 .
    AC004165 Genomic DNA. Translation: AAC16961.1 .
    CP002685 Genomic DNA. Translation: AEC08346.1 .
    AY090248 mRNA. Translation: AAL90910.1 .
    BT006363 mRNA. Translation: AAP21171.1 .
    PIRi T00587.
    RefSeqi NP_565693.1. NM_128566.3.
    UniGenei At.21347.

    3D structure databases

    ProteinModelPortali P93028.
    SMRi P93028. Positions 73-1077.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2911. 2 interactions.
    IntActi P93028. 1 interaction.
    STRINGi 3702.AT2G30110.1-P.

    Proteomic databases

    PaxDbi P93028.
    PRIDEi P93028.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G30110.1 ; AT2G30110.1 ; AT2G30110 .
    GeneIDi 817562.
    KEGGi ath:AT2G30110.

    Organism-specific databases

    TAIRi AT2G30110.

    Phylogenomic databases

    eggNOGi COG0476.
    HOGENOMi HOG000167329.
    InParanoidi P93028.
    KOi K03178.
    OMAi HISKKPV.
    PhylomeDBi P93028.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .

    Gene expression databases

    ArrayExpressi P93028.
    Genevestigatori P93028.

    Family and domain databases

    Gene3Di 1.10.3240.10. 1 hit.
    3.40.50.720. 4 hits.
    InterProi IPR009036. Molybdenum_cofac_synth_MoeB.
    IPR016040. NAD(P)-bd_dom.
    IPR000594. ThiF_NAD_FAD-bd.
    IPR018965. Ub-activating_enz_e1_C.
    IPR023280. Ub-like_act_enz_cat_cys_dom.
    IPR000127. UBact_repeat.
    IPR019572. Ubiquitin-activating_enzyme.
    IPR018075. UBQ-activ_enz_E1.
    IPR018074. UBQ-activ_enz_E1_AS.
    IPR000011. UBQ/SUMO-activ_enz_E1-like.
    [Graphical view ]
    Pfami PF00899. ThiF. 2 hits.
    PF09358. UBA_e1_C. 1 hit.
    PF10585. UBA_e1_thiolCys. 1 hit.
    PF02134. UBACT. 2 hits.
    [Graphical view ]
    PRINTSi PR01849. UBIQUITINACT.
    SMARTi SM00985. UBA_e1_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF69572. SSF69572. 2 hits.
    TIGRFAMsi TIGR01408. Ube1. 1 hit.
    PROSITEi PS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
    PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana."
      Hatfield P.M., Gosink M.M., Carpenter T.B., Vierstra R.D.
      Plant J. 11:213-226(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: cv. Columbia.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The ubiquitin pathway is required for innate immunity in Arabidopsis."
      Goritschnig S., Zhang Y., Li X.
      Plant J. 49:540-551(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF 1032-ARG--VAL-1037.

    Entry informationi

    Entry nameiUBE11_ARATH
    AccessioniPrimary (citable) accession number: P93028
    Secondary accession number(s): Q8RX82
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.
    Mutation in UBA1 (mos5) suppresses snc1-mediated constitutive resistance and affects the resistance responses conferred by only a subset of R-proteins.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3