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P93028

- UBE11_ARATH

UniProt

P93028 - UBE11_ARATH

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Protein
Ubiquitin-activating enzyme E1 1
Gene
UBA1, MOS5, At2g30110, T27E13.15
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.2 Publications

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei656 – 6561Glycyl thioester intermediate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi502 – 53130ATP By similarity
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. small protein activating enzyme activity Source: InterPro
  4. ubiquitin-protein transferase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. protein ubiquitination Source: TAIR
  2. response to cadmium ion Source: TAIR
  3. response to other organism Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-activating enzyme E1 1
Short name:
AtUBA1
Alternative name(s):
Protein MODIFIER OF SNC1 5
Gene namesi
Name:UBA1
Synonyms:MOS5
Ordered Locus Names:At2g30110
ORF Names:T27E13.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30110.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. plasma membrane Source: TAIR
  3. plasmodesma Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1032 – 10376RMDKKV → S in mos5; enhanced disease susceptibility. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10801080Ubiquitin-activating enzyme E1 1
PRO_0000399395Add
BLAST

Proteomic databases

PaxDbiP93028.
PRIDEiP93028.

Expressioni

Tissue specificityi

Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication

Developmental stagei

Expressed over the entire range of development.1 Publication

Gene expression databases

ArrayExpressiP93028.
GenevestigatoriP93028.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

BioGridi2911. 2 interactions.
IntActiP93028. 1 interaction.
STRINGi3702.AT2G30110.1-P.

Structurei

3D structure databases

ProteinModelPortaliP93028.
SMRiP93028. Positions 73-1077.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0476.
HOGENOMiHOG000167329.
InParanoidiP93028.
KOiK03178.
OMAiHISKKPV.
PhylomeDBiP93028.

Family and domain databases

Gene3Di1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProiIPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93028-1 [UniParc]FASTAAdd to Basket

« Hide

MLHKRASEAN DKNDNTIIGS DLASSKKRRI DFTESSSDKS SSILASGSSR     50
GFHGDSVVQQ IDMAFGNSNR QEIDEDLHSR QLAVYGRETM RRLFASNVLI 100
SGMHGLGAEI AKNLILAGVK SVTLHDERVV ELWDLSSNFV FSEDDVGKNR 150
ADASVQKLQD LNNAVVVSSL TKSLNKEDLS GFQVVVFSDI SMERAIEFDD 200
YCHSHQPPIA FVKADVRGLF GSVFCDFGPE FAVLDVDGEE PHTGIIASIS 250
NENQAFISCV DDERLEFEDG DLVVFSEVEG MTELNDGKPR KIKSTRPYSF 300
TLDEDTTNYG TYVKGGIVTQ VKQPKLLNFK PLREALKDPG DFLFSDFSKF 350
DRPPLLHLAF QALDHFKAEA GRFPVAGSEE DAQKLISIAT AINTGQGDLK 400
VENVDQKLLR HFSFGAKAVL NPMAAMFGGI VGQEVVKACS GKFHPLFQFF 450
YFDSVESLPS EPVDSSDFAP RNSRYDAQIS VFGAKFQKKL EDAKVFTVGS 500
GALGCEFLKN LALMGVSCGS QGKLTVTDDD IIEKSNLSRQ FLFRDWNIGQ 550
AKSTVAASAA AVINPRFNIE ALQNRVGAET ENVFDDAFWE NLTVVVNALD 600
NVNARLYVDS RCLYFQKPLL ESGTLGTKCN TQSVIPHLTE NYGASRDPPE 650
KQAPMCTVHS FPHNIDHCLT WARSEFEGLL EKTPAEVNAY LSSPVEYTNS 700
MMSAGDAQAR DTLERIVECL EKEKCETFQD CLTWARLRFE DYFVNRVKQL 750
IYTFPEDAAT STGAPFWSAP KRFPRPLQYS SSDPSLLNFI TATAILRAET 800
FGIPIPEWTK NPKEAAEAVD RVIVPDFEPR QDAKIVTDEK ATTLTTASVD 850
DAAVIDDLIA KIDQCRHNLS PDFRMKPIQF EKDDDTNYHM DVIAGLANMR 900
ARNYSIPEVD KLKAKFIAGR IIPAIATSTA MATGLVCLEL YKVLDGGHKV 950
EAYRNTFANL ALPLFSMAEP LPPKVVKHRD MAWTVWDRWV LKGNPTLREV 1000
LQWLEDKGLS AYSISCGSCL LFNSMFTRHK ERMDKKVVDL ARDVAKVELP 1050
PYRNHLDVVV ACEDEDDNDV DIPLVSIYFR 1080
Length:1,080
Mass (Da):120,251
Last modified:May 1, 1997 - v1
Checksum:i4B442A35DAFD34D0
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti883 – 8831D → G in AAP21171. 1 Publication
Sequence conflicti883 – 8831D → G in AAL90910. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80808 Genomic DNA. Translation: AAB39246.1.
AC004165 Genomic DNA. Translation: AAC16961.1.
CP002685 Genomic DNA. Translation: AEC08346.1.
AY090248 mRNA. Translation: AAL90910.1.
BT006363 mRNA. Translation: AAP21171.1.
PIRiT00587.
RefSeqiNP_565693.1. NM_128566.3.
UniGeneiAt.21347.

Genome annotation databases

EnsemblPlantsiAT2G30110.1; AT2G30110.1; AT2G30110.
GeneIDi817562.
KEGGiath:AT2G30110.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U80808 Genomic DNA. Translation: AAB39246.1 .
AC004165 Genomic DNA. Translation: AAC16961.1 .
CP002685 Genomic DNA. Translation: AEC08346.1 .
AY090248 mRNA. Translation: AAL90910.1 .
BT006363 mRNA. Translation: AAP21171.1 .
PIRi T00587.
RefSeqi NP_565693.1. NM_128566.3.
UniGenei At.21347.

3D structure databases

ProteinModelPortali P93028.
SMRi P93028. Positions 73-1077.
ModBasei Search...

Protein-protein interaction databases

BioGridi 2911. 2 interactions.
IntActi P93028. 1 interaction.
STRINGi 3702.AT2G30110.1-P.

Proteomic databases

PaxDbi P93028.
PRIDEi P93028.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G30110.1 ; AT2G30110.1 ; AT2G30110 .
GeneIDi 817562.
KEGGi ath:AT2G30110.

Organism-specific databases

TAIRi AT2G30110.

Phylogenomic databases

eggNOGi COG0476.
HOGENOMi HOG000167329.
InParanoidi P93028.
KOi K03178.
OMAi HISKKPV.
PhylomeDBi P93028.

Enzyme and pathway databases

UniPathwayi UPA00143 .

Gene expression databases

ArrayExpressi P93028.
Genevestigatori P93028.

Family and domain databases

Gene3Di 1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProi IPR009036. Molybdenum_cofac_synth_MoeB.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_e1_C.
IPR023280. Ub-like_act_enz_cat_cys_dom.
IPR000127. UBact_repeat.
IPR019572. Ubiquitin-activating_enzyme.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view ]
Pfami PF00899. ThiF. 2 hits.
PF09358. UBA_e1_C. 1 hit.
PF10585. UBA_e1_thiolCys. 1 hit.
PF02134. UBACT. 2 hits.
[Graphical view ]
PRINTSi PR01849. UBIQUITINACT.
SMARTi SM00985. UBA_e1_C. 1 hit.
[Graphical view ]
SUPFAMi SSF69572. SSF69572. 2 hits.
TIGRFAMsi TIGR01408. Ube1. 1 hit.
PROSITEi PS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana."
    Hatfield P.M., Gosink M.M., Carpenter T.B., Vierstra R.D.
    Plant J. 11:213-226(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The ubiquitin pathway is required for innate immunity in Arabidopsis."
    Goritschnig S., Zhang Y., Li X.
    Plant J. 49:540-551(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF 1032-ARG--VAL-1037.

Entry informationi

Entry nameiUBE11_ARATH
AccessioniPrimary (citable) accession number: P93028
Secondary accession number(s): Q8RX82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1997
Last modified: September 3, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.
Mutation in UBA1 (mos5) suppresses snc1-mediated constitutive resistance and affects the resistance responses conferred by only a subset of R-proteins.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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