Reviewed, UniProtKB/Swiss-Prot P93024 (ARFE_ARATH)

Names and origin

Protein names

Recommended name:
    Auxin response factor 5
Alternative name(s):
    Transcription factor MONOPTEROS
    Auxin-responsive protein IAA24

Gene names

Name:	ARF5
Synonyms:	IAA24, MP
Ordered Locus Names:	At1g19850
ORF Names:	F6F9.10

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	902 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Auxin response factors (ARFs) are transcriptional factors that binds specifically to the DNA sequence 5'-TGTCTC-3' found in the auxin-responsive promoter elements (AuxREs). Seems to act as transcriptional activator. Formation of heterodimers with Aux/IAA proteins may alter their ability to modulate early auxin response genes expression. Mediates embryo axis formation and vascular tissues differentiation. Functionally redundant with ARF7. May be necessary to counteract AMP1 activity. Ref.10
Subunit structure	Homo and heterodimers. Interacts with the auxin-responsive proteins IAA1, IAA12 (BODENLOS), IAA17 and ARF7. Ref.7 Ref.9
Subcellular location	Nucleus.
Tissue specificity	Expressed in the whole plant with a lower expression in leaves. Detected in embryo axis, provascular tissues, procambium and some differentiated vascular regions of mature organs. Ref.7
Developmental stage	In early embryo and during organ development.
Domain	Interactions between auxin response factors (ARFs) and Aux/IAA proteins occur through their C-terminal dimerization domains III and IV.
Miscellaneous	Absence of the protein probably causes early embryonic lethality. Premature stop codons are associated with vascular defects.
Sequence similarities	Belongs to the ARF family. Contains 1 Aux/IAA-ARF domain. Contains 1 TF-B3 DNA-binding domain.
Sequence caution	The sequence AAG12546.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Auxin signaling pathway Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	DNA-binding
Molecular function	Activator Developmental protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	auxin mediated signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW flower development Ref.12 Inferred from genetic interaction. Source: TAIR leaf vascular tissue pattern formation Ref.12 Inferred from genetic interaction. Source: TAIR longitudinal axis specification Inferred from mutant phenotype. Source: TAIR meristem development Ref.12 Inferred from genetic interaction. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW root development Ref.12 Inferred from genetic interaction. Source: TAIR transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from direct assay. Source: TAIR nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
IAA1	P49677	2	EBI-629519,EBI-630505

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 902	902	Auxin response factor 5		PRO_0000111509
Regions
	Domain	794 – 876	83	Aux/IAA-ARF
	DNA binding	158 – 260	103	TF-B3
	Compositional bias	23 – 28	6	Poly-Thr
	Compositional bias	282 – 289	8	Poly-Ala
	Compositional bias	445 – 499	55	Met-rich
	Compositional bias	471 – 675	205	Gln-rich
Natural variations
	Natural variant	439	1	Y → F in strain; cv. Ag-0.
	Natural variant	526	1	M → T in strain: cv. Cvi-1.
	Natural variant	539 – 542	4	Missing in strain: cv. Kas-1.
	Natural variant	796	1	T → I Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P93024-1 [UniParc]. Last modified May 1, 1999. Version 3. Checksum: D20D348369757F09	FASTA	902	99,650
	10 20 30 40 50 60 MMASLSCVED KMKTSCLVNG GGTITTTTSQ STLLEEMKLL KDQSGTRKPV INSELWHACA 70 80 90 100 110 120 GPLVCLPQVG SLVYYFSQGH SEQVAVSTRR SATTQVPNYP NLPSQLMCQV HNVTLHADKD 130 140 150 160 170 180 SDEIYAQMSL QPVHSERDVF PVPDFGMLRG SKHPTEFFCK TLTASDTSTH GGFSVPRRAA 190 200 210 220 230 240 EKLFPPLDYS AQPPTQELVV RDLHENTWTF RHIYRGQPKR HLLTTGWSLF VGSKRLRAGD 250 260 270 280 290 300 SVLFIRDEKS QLMVGVRRAN RQQTALPSSV LSADSMHIGV LAAAAHATAN RTPFLIFYNP 310 320 330 340 350 360 RACPAEFVIP LAKYRKAICG SQLSVGMRFG MMFETEDSGK RRYMGTIVGI SDLDPLRWPG 370 380 390 400 410 420 SKWRNLQVEW DEPGCNDKPT RVSPWDIETP ESLFIFPSLT SGLKRQLHPS YFAGETEWGS 430 440 450 460 470 480 LIKRPLIRVP DSANGIMPYA SFPSMASEQL MKMMMRPHNN QNVPSFMSEM QQNIVMGNGG 490 500 510 520 530 540 LLGDMKMQQP LMMNQKSEMV QPQNKLTVNP SASNTSGQEQ NLSQSMSAPA KPENSTLSGC 550 560 570 580 590 600 SSGRVQHGLE QSMEQASQVT TSTVCNEEKV NQLLQKPGAS SPVQADQCLD ITHQIYQPQS 610 620 630 640 650 660 DPINGFSFLE TDELTSQVSS FQSLAGSYKQ PFILSSQDSS AVVLPDSTNS PLFHDVWDTQ 670 680 690 700 710 720 LNGLKFDQFS PLMQQDLYAS QNICMSNSTT SNILDPPLSN TVLDDFCAIK DTDFQNHPSG 730 740 750 760 770 780 CLVGNNNTSF AQDVQSQITS ASFADSQAFS RQDFPDNSGG TGTSSSNVDF DDCSLRQNSK 790 800 810 820 830 840 GSSWQKIATP RVRTYTKVQK TGSVGRSIDV TSFKDYEELK SAIECMFGLE GLLTHPQSSG 850 860 870 880 890 900 WKLVYVDYES DVLLVGDDPW EEFVGCVRCI RILSPTEVQQ MSEEGMKLLN SAGINDLKTS VS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Arabidopsis gene MONOPTEROS encodes a transcription factor mediating embryo axis formation and vascular development." Hardtke C.S., Berleth T. EMBO J. 17:1405-1411(1998) [PubMed: 9482737] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANT ILE-796. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"The Arabidopsis STV1 protein, responsible for translation reinitiation, is required for auxin-mediated gynoecium patterning." Nishimura T., Wada T., Yamamoto K.T., Okada K. Plant Cell 17:2940-2953(2005) [PubMed: 16227452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-75. Strain: cv. Columbia.
[5]	"Protein-protein interactions among the Aux/IAA proteins." Kim J., Harter K., Theologis A. Proc. Natl. Acad. Sci. U.S.A. 94:11786-11791(1997) [PubMed: 9342315] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-902. Strain: cv. Columbia.
[6]	"The genetic architecture of shoot branching in Arabidopsis thaliana: a comparative assessment of candidate gene associations vs. quantitative trait locus mapping." Ehrenreich I.M., Stafford P.A., Purugganan M.D. Genetics 176:1223-1236(2007) [PubMed: 17435248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 422-690, VARIANTS PHE-439; THR-526 AND GLY-539--542-SER DEL. Strain: cv. Ag-0, cv. An-1, cv. Br-0, cv. C24, cv. Ct-1, cv. Cvi-1, cv. Edi-0, cv. Ga-0, cv. Kas-1, cv. Kin-0, cv. Landsberg erecta, cv. Ll-0, cv. Lz-0, cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Se-0, cv. Sorbo, cv. Tsu-1, cv. Van-0, cv. Wa-1 and cv. Wassilewskija.
[7]	"Dimerization and DNA binding of auxin response factors." Ulmasov T., Hagen G., Guilfoyle T.J. Plant J. 19:309-319(1999) [PubMed: 10476078] [Abstract] Cited for: DIMERIZATION, TISSUE SPECIFICITY.
[8]	"Activation and repression of transcription by auxin-response factors." Ulmasov T., Hagen G., Guilfoyle T.J. Proc. Natl. Acad. Sci. U.S.A. 96:5844-5849(1999) [PubMed: 10318972] [Abstract] Cited for: TRANSCRIPTIONAL ACTIVATOR.
[9]	"The Arabidopsis BODENLOS gene encodes an auxin response protein inhibiting MONOPTEROS-mediated embryo patterning." Hamann T., Benkova E., Baeurle I., Kientz M., Juergens G. Genes Dev. 16:1610-1615(2002) [PubMed: 12101120] [Abstract] Cited for: INTERACTION WITH BODENLOS.
[10]	"Auxin-responsive gene expression: genes, promoters and regulatory factors." Hagen G., Guilfoyle T.J. Plant Mol. Biol. 49:373-385(2002) [PubMed: 12036261] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION.
[11]	"Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4." Hardtke C.S., Ckurshumova W., Vidaurre D.P., Singh S.A., Stamatiou G., Tiwari S.B., Hagen G., Guilfoyle T.J., Berleth T. Development 131:1089-1100(2004) [PubMed: 14973283] [Abstract] Cited for: FUNCTION, SUBUNIT, INTERACTION WITH ARF7.
[12]	"AMP1 and MP antagonistically regulate embryo and meristem development in Arabidopsis." Vidaurre D.P., Ploense S., Krogan N.T., Berleth T. Development 134:2561-2567(2007) [PubMed: 17553903] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF037228 Genomic DNA. Translation: AAC39410.1. AF037229 mRNA. Translation: AAC60794.1. AC007797 Genomic DNA. Translation: AAG12546.1. Sequence problems. AF334716 mRNA. Translation: AAG50094.1. BT002050 mRNA. Translation: AAN72061.1. BT008805 mRNA. Translation: AAP68244.1. AB199792 mRNA. Translation: BAE48152.1. U79557 mRNA. Translation: AAB92476.1. EF598523 Genomic DNA. Translation: ABR09007.1. EF598524 Genomic DNA. Translation: ABR09008.1. EF598525 Genomic DNA. Translation: ABR09009.1. EF598526 Genomic DNA. Translation: ABR09010.1. EF598527 Genomic DNA. Translation: ABR09011.1. EF598528 Genomic DNA. Translation: ABR09012.1. EF598529 Genomic DNA. Translation: ABR09013.1. EF598530 Genomic DNA. Translation: ABR09014.1. EF598531 Genomic DNA. Translation: ABR09015.1. EF598532 Genomic DNA. Translation: ABR09016.1. EF598533 Genomic DNA. Translation: ABR09017.1. EF598534 Genomic DNA. Translation: ABR09018.1. EF598535 Genomic DNA. Translation: ABR09019.1. EF598536 Genomic DNA. Translation: ABR09020.1. EF598537 Genomic DNA. Translation: ABR09021.1. EF598538 Genomic DNA. Translation: ABR09022.1. EF598539 Genomic DNA. Translation: ABR09023.1. EF598540 Genomic DNA. Translation: ABR09024.1. EF598541 Genomic DNA. Translation: ABR09025.1. EF598542 Genomic DNA. Translation: ABR09026.1. EF598543 Genomic DNA. Translation: ABR09027.1. EF598544 Genomic DNA. Translation: ABR09028.1. EF598545 Genomic DNA. Translation: ABR09029.1. EF598546 Genomic DNA. Translation: ABR09030.1.
IPI	IPI00538603.
PIR	G86331. T51807.
RefSeq	NP_173414.1.
UniGene	At.10494
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P93024. 9 interactions.
Proteomic databases
PRIDE	P93024.
Genome annotation databases
GeneID	838573.
GenomeReviews	Gene locus AT1G19850 in contig CT485782_GR.
KEGG	ath:AT1G19850.
NMPDR	fig|3702.1.peg.2333.
Organism-specific databases
GeneFarm	3368. 345.
TAIR	At1g19850.
Phylogenomic databases
OMA	P93024. QMSEEGM.
Gene expression databases
ArrayExpress	P93024.
GermOnline	AT1G19850. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR003311. AUX_IAA. IPR011525. AuxIAA_ARF_dimer. IPR010525. Auxin_resp. IPR003340. TF_B3. [Graphical view]
Pfam	PF02309. AUX_IAA. 1 hit. PF06507. Auxin_resp. 1 hit. PF02362. B3. 1 hit. [Graphical view]
PROSITE	PS50863. B3. 1 hit. PS50962. IAA_ARF. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARFE_ARATH

Accession

Primary (citable) accession number: P93024
Secondary accession number(s): A5YYU1 , A5YYU8, A5YYW1, A5YYW2, O64965, Q33DU8, Q9FXI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)