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Protein

Regulatory protein NPR1

Gene

NPR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity). Key positive regulator of the SA-dependent signaling pathway that negatively regulates JA-dependent signaling pathway. Mediates the binding of TGA factors to the as-1 motif found in the pathogenesis-related PR-1 gene, leading to the transcriptional regulation of the gene defense. Controls the onset of systemic acquired resistance (SAR). Upon SAR induction, a biphasic change in cellular reduction potential occurs, resulting in reduction of the cytoplasmic oligomeric form to a monomeric form that accumulates in the nucleus and activates gene expression. Phosphorylated form is target of proteasome degradation.By similarity7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Biological processi

  • defense response to fungus Source: TAIR
  • induced systemic resistance Source: TAIR
  • negative regulation of defense response Source: TAIR
  • plant-type hypersensitive response Source: UniProtKB-KW
  • positive regulation of transcription, DNA-templated Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of jasmonic acid mediated signaling pathway Source: UniProtKB
  • regulation of salicylic acid mediated signaling pathway Source: UniProtKB
  • regulation of systemic acquired resistance Source: UniProtKB
  • response to bacterium Source: TAIR
  • response to heat Source: TAIR
  • response to hypoxia Source: TAIR
  • response to insect Source: TAIR
  • response to wounding Source: TAIR
  • systemic acquired resistance Source: TAIR
  • systemic acquired resistance, salicylic acid mediated signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Hypersensitive response, Plant defense, Ubl conjugation pathway

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein NPR1
Alternative name(s):
BTB/POZ domain-containing protein NPR1
Non-inducible immunity protein 1
Short name:
Nim1
Nonexpresser of PR genes 1
Salicylic acid insensitive 1
Short name:
Sai1
Gene namesi
Name:NPR1
Synonyms:NIM1, SAI1
Ordered Locus Names:At1g64280
ORF Names:F15H21.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G64280.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Accumulation in nucleus after induction by salicylic acid (SA) treatment or after pathogen infection.

GO - Cellular componenti

  • cytoplasm Source: TAIR
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi11 – 111S → A: Loss of ubiquitination and degradation; when associated with A-15. 1 Publication
Mutagenesisi15 – 151S → A: Loss of ubiquitination and degradation; when associated with A-11. 1 Publication
Mutagenesisi82 – 821C → A: Prevents oligomerization and leads to the nuclear localization. 1 Publication
Mutagenesisi150 – 1501C → Y in npr1-2; loss of SAR induction, loss of expression of PR genes and increased susceptibility to infections.
Mutagenesisi156 – 1561C → A: Abolishes S-nitrosylation and oligomerization.
Mutagenesisi216 – 2161C → A: Prevents oligomerization and leads to the nuclear localization. 1 Publication
Mutagenesisi300 – 3001H → Y in nim1-2; no induction of SAR genes expression or disease resistance.
Mutagenesisi334 – 3341H → Y in npr1-1; loss of SAR induction, loss of expression of PR genes and increased susceptibility to infections.
Mutagenesisi432 – 4321R → K in nim1-4 and nim1-5; no induction of SAR genes expression or disease resistance.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 593593Regulatory protein NPR1PRO_0000067063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111Phosphoserine1 Publication
Modified residuei15 – 151Phosphoserine1 Publication
Disulfide bondi82 – 82Interchain (with C-216); in linked form
Modified residuei156 – 1561S-nitrosocysteine1 Publication
Disulfide bondi216 – 216Interchain (with C-82); in linked form

Post-translational modificationi

Phosphorylated at Ser-11 and Ser-15 in the nucleus; facilitates its recruitment to a cullin3-based ubiquitin ligase leading to polyubiquitination and subsequent CUL3/CSN-mediated degradation.1 Publication
Ubiquitinated.1 Publication
The Cys-82-SH group reacts with Cys-216-SH of the other subunit to form an intermolecular disulfide. This disulfide might subsequently be reduced upon SAR induction.
S-nitrosylation at Cys-156 facilitates its oligomerization.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein, S-nitrosylation, Ubl conjugation

Proteomic databases

PaxDbiP93002.
PRIDEiP93002.

PTM databases

iPTMnetiP93002.

Expressioni

Inductioni

By salicylic acid (SA), benzol(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester (BTH) and 2,6-dichloroisonicotinic acid (INA).

Gene expression databases

ExpressionAtlasiP93002. baseline and differential.
GenevisibleiP93002. AT.

Interactioni

Subunit structurei

Oligomer in an uninduced state; disulfide-linked. Forms activated monomer upon SAR induction. Interacts with TGA1, TGA3, TGA4, TGA5, TGA6, TGA7 and with reduced forms of TGA1 and TGA4. Interacts with NIMIN-1, NIMIN-2 and NIMIN-3.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CUL3AQ9ZVH42EBI-1392127,EBI-531362
NIMIN-2Q9LUA32EBI-1392127,EBI-541107
TGA2P432737EBI-1392127,EBI-541307
TGA5Q391633EBI-1392127,EBI-541381

Protein-protein interaction databases

BioGridi27954. 15 interactions.
DIPiDIP-38585N.
IntActiP93002. 16 interactions.
STRINGi3702.AT1G64280.1.

Structurei

3D structure databases

ProteinModelPortaliP93002.
SMRiP93002. Positions 219-372.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini65 – 14480BTBPROSITE-ProRule annotationAdd
BLAST
Repeati265 – 29531ANK 1Add
BLAST
Repeati297 – 32428ANK 2Add
BLAST
Repeati328 – 35730ANK 3Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi537 – 55418Nuclear localization signalSequence analysisAdd
BLAST

Domaini

The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.1 Publication

Sequence similaritiesi

Contains 3 ANK repeats.PROSITE-ProRule annotation
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000240298.
InParanoidiP93002.
KOiK14508.
OMAiNKIMDAD.
OrthoDBiEOG093606Z4.
PhylomeDBiP93002.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR021094. NPR1/NIM1-like_C.
IPR024228. NPR_central_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 1 hit.
PF11900. DUF3420. 1 hit.
PF12313. NPR1_like_C. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P93002-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTTIDGFAD SYEISSTSFV ATDNTDSSIV YLAAEQVLTG PDVSALQLLS
60 70 80 90 100
NSFESVFDSP DDFYSDAKLV LSDGREVSFH RCVLSARSSF FKSALAAAKK
110 120 130 140 150
EKDSNNTAAV KLELKEIAKD YEVGFDSVVT VLAYVYSSRV RPPPKGVSEC
160 170 180 190 200
ADENCCHVAC RPAVDFMLEV LYLAFIFKIP ELITLYQRHL LDVVDKVVIE
210 220 230 240 250
DTLVILKLAN ICGKACMKLL DRCKEIIVKS NVDMVSLEKS LPEELVKEII
260 270 280 290 300
DRRKELGLEV PKVKKHVSNV HKALDSDDIE LVKLLLKEDH TNLDDACALH
310 320 330 340 350
FAVAYCNVKT ATDLLKLDLA DVNHRNPRGY TVLHVAAMRK EPQLILSLLE
360 370 380 390 400
KGASASEATL EGRTALMIAK QATMAVECNN IPEQCKHSLK GRLCVEILEQ
410 420 430 440 450
EDKREQIPRD VPPSFAVAAD ELKMTLLDLE NRVALAQRLF PTEAQAAMEI
460 470 480 490 500
AEMKGTCEFI VTSLEPDRLT GTKRTSPGVK IAPFRILEEH QSRLKALSKT
510 520 530 540 550
VELGKRFFPR CSAVLDQIMN CEDLTQLACG EDDTAEKRLQ KKQRYMEIQE
560 570 580 590
TLKKAFSEDN LELGNSSLTD STSSTSKSTG GKRSNRKLSH RRR
Length:593
Mass (Da):66,032
Last modified:May 1, 1997 - v1
Checksum:iDDD618146254CD68
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti93 – 931S → N in strain: cv. Wassilewskija. 1 Publication
Natural varianti96 – 961A → T in strain: cv. Wassilewskija. 1 Publication
Natural varianti108 – 1081Missing in strain: cv. Wassilewskija. 1 Publication
Natural varianti268 – 2681S → W in strain: cv. Wassilewskija. 1 Publication
Natural varianti406 – 4061Q → P in strain: cv. Wassilewskija. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76707 mRNA. Translation: AAC49611.1.
U87794 Genomic DNA. Translation: AAB58262.1.
AC066689 Genomic DNA. Translation: AAG51705.1.
CP002684 Genomic DNA. Translation: AEE34220.1.
AY050455 mRNA. Translation: AAK91469.1.
AY093992 mRNA. Translation: AAM16253.1.
PIRiF96666.
RefSeqiNP_176610.1. NM_105102.2.
UniGeneiAt.22448.
At.72643.

Genome annotation databases

EnsemblPlantsiAT1G64280.1; AT1G64280.1; AT1G64280.
GeneIDi842733.
GrameneiAT1G64280.1; AT1G64280.1; AT1G64280.
KEGGiath:AT1G64280.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76707 mRNA. Translation: AAC49611.1.
U87794 Genomic DNA. Translation: AAB58262.1.
AC066689 Genomic DNA. Translation: AAG51705.1.
CP002684 Genomic DNA. Translation: AEE34220.1.
AY050455 mRNA. Translation: AAK91469.1.
AY093992 mRNA. Translation: AAM16253.1.
PIRiF96666.
RefSeqiNP_176610.1. NM_105102.2.
UniGeneiAt.22448.
At.72643.

3D structure databases

ProteinModelPortaliP93002.
SMRiP93002. Positions 219-372.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27954. 15 interactions.
DIPiDIP-38585N.
IntActiP93002. 16 interactions.
STRINGi3702.AT1G64280.1.

PTM databases

iPTMnetiP93002.

Proteomic databases

PaxDbiP93002.
PRIDEiP93002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G64280.1; AT1G64280.1; AT1G64280.
GeneIDi842733.
GrameneiAT1G64280.1; AT1G64280.1; AT1G64280.
KEGGiath:AT1G64280.

Organism-specific databases

TAIRiAT1G64280.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
HOGENOMiHOG000240298.
InParanoidiP93002.
KOiK14508.
OMAiNKIMDAD.
OrthoDBiEOG093606Z4.
PhylomeDBiP93002.

Enzyme and pathway databases

UniPathwayiUPA00143.

Miscellaneous databases

PROiP93002.

Gene expression databases

ExpressionAtlasiP93002. baseline and differential.
GenevisibleiP93002. AT.

Family and domain databases

Gene3Di1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR000210. BTB/POZ_dom.
IPR021094. NPR1/NIM1-like_C.
IPR024228. NPR_central_dom.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF00651. BTB. 1 hit.
PF11900. DUF3420. 1 hit.
PF12313. NPR1_like_C. 1 hit.
[Graphical view]
SMARTiSM00248. ANK. 2 hits.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF48403. SSF48403. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 1 hit.
PS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPR1_ARATH
AccessioniPrimary (citable) accession number: P93002
Secondary accession number(s): O04742
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.