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Protein

Germin-like protein subfamily 1 member 20

Gene

GLP5A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in plant defense. Probably has no oxalate oxidase activity even if the active site is conserved.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi110 – 1101ManganeseBy similarity
Metal bindingi112 – 1121ManganeseBy similarity
Metal bindingi117 – 1171ManganeseBy similarity
Metal bindingi159 – 1591ManganeseBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Germin-like protein subfamily 1 member 20
Alternative name(s):
GLP2a copy 2
GLP2b
Germin type 2
Short name:
At-GERM2
Short name:
AtGER2
Gene namesi
Name:GLP5A
Synonyms:GER2, GERM2, GLP2A, GLP2B
Ordered Locus Names:At5g39190
ORF Names:K3K3.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G39190.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: UniProtKB-SubCell
  • extracellular matrix Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Apoplast, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 222200Germin-like protein subfamily 1 member 20PRO_0000010820Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 48By similarity
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP92996.
PRIDEiP92996.

Expressioni

Tissue specificityi

Expressed in stems and developing embryos.

Gene expression databases

ExpressionAtlasiP92996. differential.
GenevisibleiP92996. AT.

Interactioni

Subunit structurei

Oligomer (believed to be a pentamer but probably hexamer).By similarity

Protein-protein interaction databases

BioGridi19165. 1 interaction.
STRINGi3702.AT5G39190.1.

Structurei

3D structure databases

ProteinModelPortaliP92996.
SMRiP92996. Positions 24-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the germin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJA4. Eukaryota.
ENOG410YERY. LUCA.
HOGENOMiHOG000235000.
InParanoidiP92996.
OMAiGHINAVA.
PhylomeDBiP92996.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR006045. Cupin_1.
IPR001929. Germin.
IPR019780. Germin_Mn-BS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 1 hit.
[Graphical view]
PRINTSiPR00325. GERMIN.
SMARTiSM00835. Cupin_1. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00725. GERMIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P92996-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRVSQSLVPF AIIALVLSFV NAYDPSPLQD FCVAIDDLKG VFVNGRFCKD
60 70 80 90 100
PKRVDAKDFF FSGLNVPGNT NNQVGSNVTT VNVDQIPGLN TMGISLVRID
110 120 130 140 150
YAPHGQNPPH THPRGSEILV LVEGTLYVGF VSSNQDNNRL FAKVLHPGDV
160 170 180 190 200
FVFPIGMIHF QVNVGKIPAV AFAGLSSQNA GVITIANTVF GSNPPIYPEL
210 220
LARAFQLDAS VVKELQAKFG SI
Length:222
Mass (Da):23,925
Last modified:May 1, 1997 - v1
Checksum:iE2BD7EDA9D47948C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti63 – 631G → A in CAA63023 (PubMed:9349269).Curated
Sequence conflicti98 – 992RI → LV in CAA63023 (PubMed:9349269).Curated
Sequence conflicti107 – 1071N → D in CAA63023 (PubMed:9349269).Curated
Sequence conflicti135 – 1351Q → L in CAA63023 (PubMed:9349269).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91957 mRNA. Translation: CAA63023.1.
U75192 mRNA. Translation: AAB51570.1.
AB010694 Genomic DNA. Translation: BAB09373.1.
CP002688 Genomic DNA. Translation: AED94405.1.
BT003986 mRNA. Translation: AAO42026.1.
BT005069 mRNA. Translation: AAO50602.1.
PIRiS71254.
RefSeqiNP_198735.1. NM_123281.3. [P92996-1]
UniGeneiAt.49106.
At.65.

Genome annotation databases

EnsemblPlantsiAT5G39190.1; AT5G39190.1; AT5G39190. [P92996-1]
GeneIDi833914.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91957 mRNA. Translation: CAA63023.1.
U75192 mRNA. Translation: AAB51570.1.
AB010694 Genomic DNA. Translation: BAB09373.1.
CP002688 Genomic DNA. Translation: AED94405.1.
BT003986 mRNA. Translation: AAO42026.1.
BT005069 mRNA. Translation: AAO50602.1.
PIRiS71254.
RefSeqiNP_198735.1. NM_123281.3. [P92996-1]
UniGeneiAt.49106.
At.65.

3D structure databases

ProteinModelPortaliP92996.
SMRiP92996. Positions 24-220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi19165. 1 interaction.
STRINGi3702.AT5G39190.1.

Proteomic databases

PaxDbiP92996.
PRIDEiP92996.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G39190.1; AT5G39190.1; AT5G39190. [P92996-1]
GeneIDi833914.

Organism-specific databases

TAIRiAT5G39190.

Phylogenomic databases

eggNOGiENOG410IJA4. Eukaryota.
ENOG410YERY. LUCA.
HOGENOMiHOG000235000.
InParanoidiP92996.
OMAiGHINAVA.
PhylomeDBiP92996.

Miscellaneous databases

PROiP92996.

Gene expression databases

ExpressionAtlasiP92996. differential.
GenevisibleiP92996. AT.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR006045. Cupin_1.
IPR001929. Germin.
IPR019780. Germin_Mn-BS.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF00190. Cupin_1. 1 hit.
[Graphical view]
PRINTSiPR00325. GERMIN.
SMARTiSM00835. Cupin_1. 1 hit.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
PROSITEiPS00725. GERMIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence, genomic organization and differential expression of three Arabidopsis genes for germin/oxalate oxidase-like proteins."
    Membre N., Berna A., Neutelings G., David A., David H., Staiger D., Saez Vasquez J., Raynal M., Delseny M., Bernier F.
    Plant Mol. Biol. 35:459-469(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Arabidopsis thaliana contains a large family of germin-like proteins: characterization of cDNA and genomic sequences encoding 12 unique family members."
    Carter C., Graham R.A., Thornburg R.W.
    Plant Mol. Biol. 38:929-943(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  3. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Arabidopsis thaliana germin-like proteins: common and specific features point to a variety of functions."
    Membre N., Bernier F., Staiger D., Berna A.
    Planta 211:345-354(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiGL120_ARATH
AccessioniPrimary (citable) accession number: P92996
Secondary accession number(s): Q39106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: May 1, 1997
Last modified: May 11, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.