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P92994

- TCMO_ARATH

UniProt

P92994 - TCMO_ARATH

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Protein
Trans-cinnamate 4-monooxygenase
Gene
CYP73A5, CYP73, At2g30490, T6B20.16
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.

Catalytic activityi

Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.

Cofactori

Heme group By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. identical protein binding Source: TAIR
  3. iron ion binding Source: InterPro
  4. protein binding Source: TAIR
  5. trans-cinnamate 4-monooxygenase activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. developmental process Source: TAIR
  2. growth Source: TAIR
  3. lignin metabolic process Source: TAIR
  4. phenylpropanoid metabolic process Source: TAIR
  5. pollen development Source: TAIR
  6. response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT2G30490-MONOMER.
UniPathwayiUPA00825; UER00789.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-cinnamate 4-monooxygenase (EC:1.14.13.11)
Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name:
C4H
Short name:
CA4H
Cytochrome P450 73
Cytochrome P450C4H
Gene namesi
Name:CYP73A5
Synonyms:CYP73
Ordered Locus Names:At2g30490
ORF Names:T6B20.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30490.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: TAIR
  2. endoplasmic reticulum Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: TAIR
  5. plant-type cell wall Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
  8. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Trans-cinnamate 4-monooxygenase
PRO_0000052242Add
BLAST

Proteomic databases

PaxDbiP92994.
PRIDEiP92994.

Expressioni

Gene expression databases

ArrayExpressiP92994.
GenevestigatoriP92994.

Interactioni

Protein-protein interaction databases

BioGridi2948. 3 interactions.
IntActiP92994. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP92994.
SMRiP92994. Positions 30-504.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000218629.
InParanoidiP92994.
KOiK00487.
OMAiGITIGRM.
PhylomeDBiP92994.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92994-1 [UniParc]FASTAAdd to Basket

« Hide

MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG    50
DDLNHRNLVD YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS 100
RTRNVVFDIF TGKGQDMVFT VYGEHWRKMR RIMTVPFFTN KVVQQNREGW 150
EFEAASVVED VKKNPDSATK GIVLRKRLQL MMYNNMFRIM FDRRFESEDD 200
PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK ICQDVKDRRI 250
ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV 300
ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD 350
LHKLPYLQAV VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA 400
WWLANNPNSW KKPEEFRPER FFEEESHVEA NGNDFRYVPF GVGRRSCPGI 450
ILALPILGIT IGRMVQNFEL LPPPGQSKVD TSEKGGQFSL HILNHSIIVM 500
KPRNC 505
Length:505
Mass (Da):57,792
Last modified:May 1, 1997 - v1
Checksum:i61DEAAA9463362CF
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921L → H in strain: cv. Landsberg erecta.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841N → T in BAA24355. 1 Publication
Sequence conflicti333 – 3331R → G in BAA24355. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U71081 mRNA. Translation: AAB58356.1.
U71080 Genomic DNA. Translation: AAB58355.1.
D78596 mRNA. Translation: BAA24355.1.
U93215 Genomic DNA. Translation: AAB63088.1.
CP002685 Genomic DNA. Translation: AEC08397.1.
AY065145 mRNA. Translation: AAL38321.1.
BT008875 mRNA. Translation: AAP68314.1.
PIRiA84709.
RefSeqiNP_180607.1. NM_128601.2.
UniGeneiAt.23450.

Genome annotation databases

EnsemblPlantsiAT2G30490.1; AT2G30490.1; AT2G30490.
GeneIDi817599.
KEGGiath:AT2G30490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U71081 mRNA. Translation: AAB58356.1 .
U71080 Genomic DNA. Translation: AAB58355.1 .
D78596 mRNA. Translation: BAA24355.1 .
U93215 Genomic DNA. Translation: AAB63088.1 .
CP002685 Genomic DNA. Translation: AEC08397.1 .
AY065145 mRNA. Translation: AAL38321.1 .
BT008875 mRNA. Translation: AAP68314.1 .
PIRi A84709.
RefSeqi NP_180607.1. NM_128601.2.
UniGenei At.23450.

3D structure databases

ProteinModelPortali P92994.
SMRi P92994. Positions 30-504.
ModBasei Search...

Protein-protein interaction databases

BioGridi 2948. 3 interactions.
IntActi P92994. 2 interactions.

Proteomic databases

PaxDbi P92994.
PRIDEi P92994.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G30490.1 ; AT2G30490.1 ; AT2G30490 .
GeneIDi 817599.
KEGGi ath:AT2G30490.

Organism-specific databases

GeneFarmi 1333. 94.
TAIRi AT2G30490.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000218629.
InParanoidi P92994.
KOi K00487.
OMAi GITIGRM.
PhylomeDBi P92994.

Enzyme and pathway databases

UniPathwayi UPA00825 ; UER00789 .
BioCyci MetaCyc:AT2G30490-MONOMER.

Gene expression databases

ArrayExpressi P92994.
Genevestigatori P92994.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment."
    Bell-Lelong D.A., Cusumano J.C., Meyer K., Chapple C.
    Plant Physiol. 113:729-738(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta."
    Mizutani M., Ohta D., Sato R.
    Plant Physiol. 113:755-763(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
    Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
    Plant Physiol. Biochem. 0:0-0(2013)
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiTCMO_ARATH
AccessioniPrimary (citable) accession number: P92994
Secondary accession number(s): O04995, O49834, P92993
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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