Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trans-cinnamate 4-monooxygenase

Gene

CYP73A5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.

Catalytic activityi

Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. identical protein binding Source: TAIR
  3. iron ion binding Source: InterPro
  4. trans-cinnamate 4-monooxygenase activity Source: TAIR

GO - Biological processi

  1. developmental process Source: TAIR
  2. growth Source: TAIR
  3. lignin metabolic process Source: TAIR
  4. phenylpropanoid metabolic process Source: TAIR
  5. pollen development Source: TAIR
  6. response to karrikin Source: TAIR
  7. secondary metabolite biosynthetic process Source: GO_Central
  8. ubiquinone biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciMetaCyc:AT2G30490-MONOMER.
SABIO-RKP92994.
UniPathwayiUPA00825; UER00789.

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-cinnamate 4-monooxygenase (EC:1.14.13.11)
Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name:
C4H
Short name:
CA4H
Cytochrome P450 73
Cytochrome P450C4H
Gene namesi
Name:CYP73A5
Synonyms:CYP73
Ordered Locus Names:At2g30490
ORF Names:T6B20.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G30490.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei3 – 2321HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: TAIR
  2. Golgi apparatus Source: TAIR
  3. integral component of membrane Source: UniProtKB-KW
  4. membrane Source: TAIR
  5. plant-type cell wall Source: TAIR
  6. plasma membrane Source: TAIR
  7. plasmodesma Source: TAIR
  8. vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Trans-cinnamate 4-monooxygenasePRO_0000052242Add
BLAST

Proteomic databases

PaxDbiP92994.
PRIDEiP92994.

Expressioni

Gene expression databases

ExpressionAtlasiP92994. baseline and differential.
GenevestigatoriP92994.

Interactioni

Protein-protein interaction databases

BioGridi2948. 3 interactions.
IntActiP92994. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP92994.
SMRiP92994. Positions 30-484.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000218629.
InParanoidiP92994.
KOiK00487.
OMAiGITIGRM.
PhylomeDBiP92994.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92994-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG
60 70 80 90 100
DDLNHRNLVD YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS
110 120 130 140 150
RTRNVVFDIF TGKGQDMVFT VYGEHWRKMR RIMTVPFFTN KVVQQNREGW
160 170 180 190 200
EFEAASVVED VKKNPDSATK GIVLRKRLQL MMYNNMFRIM FDRRFESEDD
210 220 230 240 250
PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK ICQDVKDRRI
260 270 280 290 300
ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV
310 320 330 340 350
ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD
360 370 380 390 400
LHKLPYLQAV VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA
410 420 430 440 450
WWLANNPNSW KKPEEFRPER FFEEESHVEA NGNDFRYVPF GVGRRSCPGI
460 470 480 490 500
ILALPILGIT IGRMVQNFEL LPPPGQSKVD TSEKGGQFSL HILNHSIIVM

KPRNC
Length:505
Mass (Da):57,792
Last modified:May 1, 1997 - v1
Checksum:i61DEAAA9463362CF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti184 – 1841N → T in BAA24355. (PubMed:9085571)Curated
Sequence conflicti333 – 3331R → G in BAA24355. (PubMed:9085571)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti92 – 921L → H in strain: cv. Landsberg erecta.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71081 mRNA. Translation: AAB58356.1.
U71080 Genomic DNA. Translation: AAB58355.1.
D78596 mRNA. Translation: BAA24355.1.
U93215 Genomic DNA. Translation: AAB63088.1.
CP002685 Genomic DNA. Translation: AEC08397.1.
AY065145 mRNA. Translation: AAL38321.1.
BT008875 mRNA. Translation: AAP68314.1.
PIRiA84709.
RefSeqiNP_180607.1. NM_128601.2.
UniGeneiAt.23450.

Genome annotation databases

EnsemblPlantsiAT2G30490.1; AT2G30490.1; AT2G30490.
GeneIDi817599.
KEGGiath:AT2G30490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71081 mRNA. Translation: AAB58356.1.
U71080 Genomic DNA. Translation: AAB58355.1.
D78596 mRNA. Translation: BAA24355.1.
U93215 Genomic DNA. Translation: AAB63088.1.
CP002685 Genomic DNA. Translation: AEC08397.1.
AY065145 mRNA. Translation: AAL38321.1.
BT008875 mRNA. Translation: AAP68314.1.
PIRiA84709.
RefSeqiNP_180607.1. NM_128601.2.
UniGeneiAt.23450.

3D structure databases

ProteinModelPortaliP92994.
SMRiP92994. Positions 30-484.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi2948. 3 interactions.
IntActiP92994. 2 interactions.

Proteomic databases

PaxDbiP92994.
PRIDEiP92994.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G30490.1; AT2G30490.1; AT2G30490.
GeneIDi817599.
KEGGiath:AT2G30490.

Organism-specific databases

GeneFarmi1333. 94.
TAIRiAT2G30490.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000218629.
InParanoidiP92994.
KOiK00487.
OMAiGITIGRM.
PhylomeDBiP92994.

Enzyme and pathway databases

UniPathwayiUPA00825; UER00789.
BioCyciMetaCyc:AT2G30490-MONOMER.
SABIO-RKP92994.

Gene expression databases

ExpressionAtlasiP92994. baseline and differential.
GenevestigatoriP92994.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment."
    Bell-Lelong D.A., Cusumano J.C., Meyer K., Chapple C.
    Plant Physiol. 113:729-738(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: cv. Columbia and cv. Landsberg erecta.
  2. "Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta."
    Mizutani M., Ohta D., Sato R.
    Plant Physiol. 113:755-763(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
    Tissue: Seedling.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
    Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
    Plant Physiol. Biochem. 0:0-0(2013)
    Cited for: REVIEW, NOMENCLATURE.

Entry informationi

Entry nameiTCMO_ARATH
AccessioniPrimary (citable) accession number: P92994
Secondary accession number(s): O04995, O49834, P92993
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: February 4, 2015
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.