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P92994 (TCMO_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trans-cinnamate 4-monooxygenase

EC=1.14.13.11
Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H
Gene names
Name:CYP73A5
Synonyms:CYP73
Ordered Locus Names:At2g30490
ORF Names:T6B20.16
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.

Catalytic activity

Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.

Cofactor

Heme group By similarity.

Pathway

Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.

Subcellular location

Membrane; Single-pass membrane protein Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processdevelopmental process

Inferred from mutant phenotype PubMed 19682296. Source: TAIR

growth

Inferred from mutant phenotype PubMed 19682296. Source: TAIR

lignin metabolic process

Inferred from mutant phenotype PubMed 19682296. Source: TAIR

phenylpropanoid metabolic process

Inferred from mutant phenotype PubMed 19682296. Source: TAIR

pollen development

Inferred from mutant phenotype PubMed 19682296. Source: TAIR

response to karrikin

Inferred from expression pattern PubMed 20351290. Source: TAIR

   Cellular_componentGolgi apparatus

Inferred from direct assay PubMed 22430844. Source: TAIR

endoplasmic reticulum

Inferred from direct assay PubMed 16618929PubMed 22923678PubMed 23175744. Source: TAIR

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

membrane

Inferred from direct assay PubMed 17432890. Source: TAIR

plant-type cell wall

Inferred from direct assay PubMed 16287169. Source: TAIR

plasma membrane

Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR

plasmodesma

Inferred from direct assay PubMed 21533090. Source: TAIR

vacuolar membrane

Inferred from direct assay PubMed 17151019. Source: TAIR

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

identical protein binding

Inferred from physical interaction PubMed 23175744. Source: TAIR

iron ion binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 23175744. Source: TAIR

trans-cinnamate 4-monooxygenase activity

Inferred from direct assay Ref.2. Source: TAIR

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 505505Trans-cinnamate 4-monooxygenase
PRO_0000052242

Regions

Transmembrane3 – 2321Helical; Potential

Sites

Metal binding4471Iron (heme axial ligand) By similarity

Natural variations

Natural variant921L → H in strain: cv. Landsberg erecta.

Experimental info

Sequence conflict1841N → T in BAA24355. Ref.2
Sequence conflict3331R → G in BAA24355. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P92994 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 61DEAAA9463362CF

FASTA50557,792
        10         20         30         40         50         60 
MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG DDLNHRNLVD 

        70         80         90        100        110        120 
YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS RTRNVVFDIF TGKGQDMVFT 

       130        140        150        160        170        180 
VYGEHWRKMR RIMTVPFFTN KVVQQNREGW EFEAASVVED VKKNPDSATK GIVLRKRLQL 

       190        200        210        220        230        240 
MMYNNMFRIM FDRRFESEDD PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK 

       250        260        270        280        290        300 
ICQDVKDRRI ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV 

       310        320        330        340        350        360 
ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD LHKLPYLQAV 

       370        380        390        400        410        420 
VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA WWLANNPNSW KKPEEFRPER 

       430        440        450        460        470        480 
FFEEESHVEA NGNDFRYVPF GVGRRSCPGI ILALPILGIT IGRMVQNFEL LPPPGQSKVD 

       490        500 
TSEKGGQFSL HILNHSIIVM KPRNC 

« Hide

References

« Hide 'large scale' references
[1]"Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment."
Bell-Lelong D.A., Cusumano J.C., Meyer K., Chapple C.
Plant Physiol. 113:729-738(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: cv. Columbia and cv. Landsberg erecta.
[2]"Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta."
Mizutani M., Ohta D., Sato R.
Plant Physiol. 113:755-763(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Seedling.
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[6]"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
Plant Physiol. Biochem. 0:0-0(2013)
Cited for: REVIEW, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U71081 mRNA. Translation: AAB58356.1.
U71080 Genomic DNA. Translation: AAB58355.1.
D78596 mRNA. Translation: BAA24355.1.
U93215 Genomic DNA. Translation: AAB63088.1.
CP002685 Genomic DNA. Translation: AEC08397.1.
AY065145 mRNA. Translation: AAL38321.1.
BT008875 mRNA. Translation: AAP68314.1.
PIRA84709.
RefSeqNP_180607.1. NM_128601.2.
UniGeneAt.23450.

3D structure databases

ProteinModelPortalP92994.
SMRP92994. Positions 30-504.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid2948. 3 interactions.
IntActP92994. 2 interactions.

Proteomic databases

PaxDbP92994.
PRIDEP92994.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G30490.1; AT2G30490.1; AT2G30490.
GeneID817599.
KEGGath:AT2G30490.

Organism-specific databases

GeneFarm1333. 94.
TAIRAT2G30490.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000218629.
InParanoidP92994.
KOK00487.
OMAGITIGRM.
PhylomeDBP92994.

Enzyme and pathway databases

BioCycMetaCyc:AT2G30490-MONOMER.
UniPathwayUPA00825; UER00789.

Gene expression databases

ArrayExpressP92994.
GenevestigatorP92994.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTCMO_ARATH
AccessionPrimary (citable) accession number: P92994
Secondary accession number(s): O04995, O49834, P92993
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names