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P92994

- TCMO_ARATH

UniProt

P92994 - TCMO_ARATH

Protein

Trans-cinnamate 4-monooxygenase

Gene

CYP73A5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.

    Catalytic activityi

    Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.

    Cofactori

    Heme group.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi447 – 4471Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. identical protein binding Source: TAIR
    3. iron ion binding Source: InterPro
    4. protein binding Source: TAIR
    5. trans-cinnamate 4-monooxygenase activity Source: TAIR

    GO - Biological processi

    1. developmental process Source: TAIR
    2. growth Source: TAIR
    3. lignin metabolic process Source: TAIR
    4. phenylpropanoid metabolic process Source: TAIR
    5. pollen development Source: TAIR
    6. response to karrikin Source: TAIR

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:AT2G30490-MONOMER.
    UniPathwayiUPA00825; UER00789.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trans-cinnamate 4-monooxygenase (EC:1.14.13.11)
    Alternative name(s):
    Cinnamic acid 4-hydroxylase
    Short name:
    C4H
    Short name:
    CA4H
    Cytochrome P450 73
    Cytochrome P450C4H
    Gene namesi
    Name:CYP73A5
    Synonyms:CYP73
    Ordered Locus Names:At2g30490
    ORF Names:T6B20.16
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G30490.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum Source: TAIR
    2. Golgi apparatus Source: TAIR
    3. integral component of membrane Source: UniProtKB-KW
    4. membrane Source: TAIR
    5. plant-type cell wall Source: TAIR
    6. plasma membrane Source: TAIR
    7. plasmodesma Source: TAIR
    8. vacuolar membrane Source: TAIR

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 505505Trans-cinnamate 4-monooxygenasePRO_0000052242Add
    BLAST

    Proteomic databases

    PaxDbiP92994.
    PRIDEiP92994.

    Expressioni

    Gene expression databases

    ArrayExpressiP92994.
    GenevestigatoriP92994.

    Interactioni

    Protein-protein interaction databases

    BioGridi2948. 3 interactions.
    IntActiP92994. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP92994.
    SMRiP92994. Positions 30-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000218629.
    InParanoidiP92994.
    KOiK00487.
    OMAiGITIGRM.
    PhylomeDBiP92994.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P92994-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG    50
    DDLNHRNLVD YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS 100
    RTRNVVFDIF TGKGQDMVFT VYGEHWRKMR RIMTVPFFTN KVVQQNREGW 150
    EFEAASVVED VKKNPDSATK GIVLRKRLQL MMYNNMFRIM FDRRFESEDD 200
    PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK ICQDVKDRRI 250
    ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV 300
    ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD 350
    LHKLPYLQAV VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA 400
    WWLANNPNSW KKPEEFRPER FFEEESHVEA NGNDFRYVPF GVGRRSCPGI 450
    ILALPILGIT IGRMVQNFEL LPPPGQSKVD TSEKGGQFSL HILNHSIIVM 500
    KPRNC 505
    Length:505
    Mass (Da):57,792
    Last modified:May 1, 1997 - v1
    Checksum:i61DEAAA9463362CF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti184 – 1841N → T in BAA24355. (PubMed:9085571)Curated
    Sequence conflicti333 – 3331R → G in BAA24355. (PubMed:9085571)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti92 – 921L → H in strain: cv. Landsberg erecta.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U71081 mRNA. Translation: AAB58356.1.
    U71080 Genomic DNA. Translation: AAB58355.1.
    D78596 mRNA. Translation: BAA24355.1.
    U93215 Genomic DNA. Translation: AAB63088.1.
    CP002685 Genomic DNA. Translation: AEC08397.1.
    AY065145 mRNA. Translation: AAL38321.1.
    BT008875 mRNA. Translation: AAP68314.1.
    PIRiA84709.
    RefSeqiNP_180607.1. NM_128601.2.
    UniGeneiAt.23450.

    Genome annotation databases

    EnsemblPlantsiAT2G30490.1; AT2G30490.1; AT2G30490.
    GeneIDi817599.
    KEGGiath:AT2G30490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U71081 mRNA. Translation: AAB58356.1 .
    U71080 Genomic DNA. Translation: AAB58355.1 .
    D78596 mRNA. Translation: BAA24355.1 .
    U93215 Genomic DNA. Translation: AAB63088.1 .
    CP002685 Genomic DNA. Translation: AEC08397.1 .
    AY065145 mRNA. Translation: AAL38321.1 .
    BT008875 mRNA. Translation: AAP68314.1 .
    PIRi A84709.
    RefSeqi NP_180607.1. NM_128601.2.
    UniGenei At.23450.

    3D structure databases

    ProteinModelPortali P92994.
    SMRi P92994. Positions 30-504.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 2948. 3 interactions.
    IntActi P92994. 2 interactions.

    Proteomic databases

    PaxDbi P92994.
    PRIDEi P92994.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G30490.1 ; AT2G30490.1 ; AT2G30490 .
    GeneIDi 817599.
    KEGGi ath:AT2G30490.

    Organism-specific databases

    GeneFarmi 1333. 94.
    TAIRi AT2G30490.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000218629.
    InParanoidi P92994.
    KOi K00487.
    OMAi GITIGRM.
    PhylomeDBi P92994.

    Enzyme and pathway databases

    UniPathwayi UPA00825 ; UER00789 .
    BioCyci MetaCyc:AT2G30490-MONOMER.

    Gene expression databases

    ArrayExpressi P92994.
    Genevestigatori P92994.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment."
      Bell-Lelong D.A., Cusumano J.C., Meyer K., Chapple C.
      Plant Physiol. 113:729-738(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: cv. Columbia and cv. Landsberg erecta.
    2. "Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta."
      Mizutani M., Ohta D., Sato R.
      Plant Physiol. 113:755-763(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
      Tissue: Seedling.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    6. "The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity."
      Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R.
      Plant Physiol. Biochem. 0:0-0(2013)
      Cited for: REVIEW, NOMENCLATURE.

    Entry informationi

    Entry nameiTCMO_ARATH
    AccessioniPrimary (citable) accession number: P92994
    Secondary accession number(s): O04995, O49834, P92993
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3