P92994 (TCMO_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trans-cinnamate 4-monooxygenase
EC=1.14.13.11

Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name=C4H
Short name=CA4H
Cytochrome P450 73
Cytochrome P450C4H

Gene names

Name:	CYP73A5
Synonyms:	CYP73
Ordered Locus Names:	At2g30490
ORF Names:	T6B20.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Subcellular location	Membrane; Single-pass membrane protein Potential.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth Inferred from mutant phenotype PubMed 19682296. Source: TAIR lignin metabolic process Inferred from mutant phenotype PubMed 19682296. Source: TAIR pollen development Inferred from mutant phenotype PubMed 19682296. Source: TAIR response to karrikin Inferred from expression pattern PubMed 20351290. Source: TAIR
Cellular_component	Golgi apparatus Inferred from direct assay PubMed 22430844. Source: TAIR endoplasmic reticulum Inferred from direct assay PubMed 16618929PubMed 22923678PubMed 23175744. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type cell wall Inferred from direct assay PubMed 16287169. Source: TAIR plasma membrane Inferred from direct assay PubMed 17317660PubMed 17644812. Source: TAIR plasmodesma Inferred from direct assay PubMed 21533090. Source: TAIR vacuolar membrane Inferred from direct assay PubMed 17151019. Source: TAIR
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from direct assay Ref.2. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 505	505	Trans-cinnamate 4-monooxygenase		PRO_0000052242
Regions
	Transmembrane	3 – 23	21	Helical; Potential
Sites
	Metal binding	447	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	92	1	L → H in strain: cv. Landsberg erecta.
Experimental info
	Sequence conflict	184	1	N → T in BAA24355. Ref.2
	Sequence conflict	333	1	R → G in BAA24355. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P92994 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 61DEAAA9463362CF	FASTA	505	57,792
	10 20 30 40 50 60 MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG DDLNHRNLVD 70 80 90 100 110 120 YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQNREGW EFEAASVVED VKKNPDSATK GIVLRKRLQL 190 200 210 220 230 240 MMYNNMFRIM FDRRFESEDD PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK 250 260 270 280 290 300 ICQDVKDRRI ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV 310 320 330 340 350 360 ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD LHKLPYLQAV 370 380 390 400 410 420 VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA WWLANNPNSW KKPEEFRPER 430 440 450 460 470 480 FFEEESHVEA NGNDFRYVPF GVGRRSCPGI ILALPILGIT IGRMVQNFEL LPPPGQSKVD 490 500 TSEKGGQFSL HILNHSIIVM KPRNC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cinnamate-4-hydroxylase expression in Arabidopsis. Regulation in response to development and the environment." Bell-Lelong D.A., Cusumano J.C., Meyer K., Chapple C. Plant Physiol. 113:729-738(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia and cv. Landsberg erecta.
[2]	"Isolation of a cDNA and a genomic clone encoding cinnamate 4-hydroxylase from Arabidopsis and its expression manner in planta." Mizutani M., Ohta D., Sato R. Plant Physiol. 113:755-763(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Seedling.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"The flavonoid biosynthetic pathway in Arabidopsis: Structural and genetic diversity." Saito K., Yonekura-Sakakibara K., Nakabayashi R., Higashi Y., Yamazaki M., Tohge T., Fernie A.R. Plant Physiol. Biochem. 0:0-0(2013) Cited for: REVIEW, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U71081 mRNA. Translation: AAB58356.1. U71080 Genomic DNA. Translation: AAB58355.1. D78596 mRNA. Translation: BAA24355.1. U93215 Genomic DNA. Translation: AAB63088.1. CP002685 Genomic DNA. Translation: AEC08397.1. AY065145 mRNA. Translation: AAL38321.1. BT008875 mRNA. Translation: AAP68314.1.
IPI	IPI00525264.
PIR	A84709.
RefSeq	NP_180607.1. NM_128601.2.
UniGene	At.23450.
3D structure databases
ProteinModelPortal	P92994.
SMR	P92994. Positions 30-504.
ModBase	Search...
Protein-protein interaction databases
IntAct	P92994. 2 interactions.
Proteomic databases
PaxDb	P92994.
PRIDE	P92994.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G30490.1; AT2G30490.1; AT2G30490.
GeneID	817599.
KEGG	ath:AT2G30490.
Organism-specific databases
GeneFarm	1333. 94.
TAIR	At2g30490.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000218629.
InParanoid	P92994.
KO	K00487.
OMA	GVQVTEP.
PhylomeDB	P92994.
ProtClustDB	PLN02394.
Enzyme and pathway databases
BioCyc	MetaCyc:AT2G30490-MONOMER.
UniPathway	UPA00825; UER00789.
Gene expression databases
ArrayExpress	P92994.
Genevestigator	P92994.
GermOnline	AT2G30490. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_ARATH

Accession

Primary (citable) accession number: P92994
Secondary accession number(s): O04995, O49834, P92993

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 114 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program