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Protein

Trans-cinnamate 4-monooxygenase

Gene

CYP73A5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.

Catalytic activityi

Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.

Cofactori

hemeBy similarity

Pathwayi: trans-4-coumarate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes trans-4-coumarate from trans-cinnamate.
Proteins known to be involved in this subpathway in this organism are:
  1. Trans-cinnamate 4-monooxygenase (CYP73A5)
This subpathway is part of the pathway trans-4-coumarate biosynthesis, which is itself part of Phenylpropanoid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes trans-4-coumarate from trans-cinnamate, the pathway trans-4-coumarate biosynthesis and in Phenylpropanoid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi447Iron (heme axial ligand)By similarity1

GO - Molecular functioni

  • heme binding Source: InterPro
  • identical protein binding Source: TAIR
  • iron ion binding Source: InterPro
  • trans-cinnamate 4-monooxygenase activity Source: TAIR

GO - Biological processi

  • developmental process Source: TAIR
  • growth Source: TAIR
  • lignin metabolic process Source: TAIR
  • phenylpropanoid metabolic process Source: TAIR
  • pollen development Source: TAIR
  • response to karrikin Source: TAIR
  • ubiquinone biosynthetic process Source: TAIR

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandHeme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BioCyciARA:AT2G30490-MONOMER
MetaCyc:AT2G30490-MONOMER
SABIO-RKiP92994
UniPathwayiUPA00825; UER00789

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-cinnamate 4-monooxygenase (EC:1.14.13.11)
Alternative name(s):
Cinnamic acid 4-hydroxylase
Short name:
C4H
Short name:
CA4H
Cytochrome P450 73
Cytochrome P450C4H
Gene namesi
Name:CYP73A5
Synonyms:CYP73
Ordered Locus Names:At2g30490
ORF Names:T6B20.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G30490
TAIRilocus:2064402 AT2G30490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei3 – 23HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000522421 – 505Trans-cinnamate 4-monooxygenaseAdd BLAST505

Proteomic databases

PaxDbiP92994
PRIDEiP92994

Expressioni

Gene expression databases

ExpressionAtlasiP92994 baseline and differential
GenevisibleiP92994 AT

Interactioni

GO - Molecular functioni

  • identical protein binding Source: TAIR

Protein-protein interaction databases

BioGridi2948, 8 interactors
IntActiP92994, 2 interactors
STRINGi3702.AT2G30490.1

Structurei

3D structure databases

ProteinModelPortaliP92994
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0156 Eukaryota
COG2124 LUCA
HOGENOMiHOG000218629
InParanoidiP92994
KOiK00487
OMAiNNWQDYI
OrthoDBiEOG093609TK
PhylomeDBiP92994

Family and domain databases

Gene3Di1.10.630.10, 1 hit
InterProiView protein in InterPro
IPR001128 Cyt_P450
IPR017972 Cyt_P450_CS
IPR002401 Cyt_P450_E_grp-I
IPR036396 Cyt_P450_sf
PfamiView protein in Pfam
PF00067 p450, 1 hit
PRINTSiPR00463 EP450I
PR00385 P450
SUPFAMiSSF48264 SSF48264, 1 hit
PROSITEiView protein in PROSITE
PS00086 CYTOCHROME_P450, 1 hit

Sequencei

Sequence statusi: Complete.

P92994-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLLLLEKSL IAVFVAVILA TVISKLRGKK LKLPPGPIPI PIFGNWLQVG
60 70 80 90 100
DDLNHRNLVD YAKKFGDLFL LRMGQRNLVV VSSPDLTKEV LLTQGVEFGS
110 120 130 140 150
RTRNVVFDIF TGKGQDMVFT VYGEHWRKMR RIMTVPFFTN KVVQQNREGW
160 170 180 190 200
EFEAASVVED VKKNPDSATK GIVLRKRLQL MMYNNMFRIM FDRRFESEDD
210 220 230 240 250
PLFLRLKALN GERSRLAQSF EYNYGDFIPI LRPFLRGYLK ICQDVKDRRI
260 270 280 290 300
ALFKKYFVDE RKQIASSKPT GSEGLKCAID HILEAEQKGE INEDNVLYIV
310 320 330 340 350
ENINVAAIET TLWSIEWGIA ELVNHPEIQS KLRNELDTVL GPGVQVTEPD
360 370 380 390 400
LHKLPYLQAV VKETLRLRMA IPLLVPHMNL HDAKLAGYDI PAESKILVNA
410 420 430 440 450
WWLANNPNSW KKPEEFRPER FFEEESHVEA NGNDFRYVPF GVGRRSCPGI
460 470 480 490 500
ILALPILGIT IGRMVQNFEL LPPPGQSKVD TSEKGGQFSL HILNHSIIVM

KPRNC
Length:505
Mass (Da):57,792
Last modified:May 1, 1997 - v1
Checksum:i61DEAAA9463362CF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti184N → T in BAA24355 (PubMed:9085571).Curated1
Sequence conflicti333R → G in BAA24355 (PubMed:9085571).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti92L → H in strain: cv. Landsberg erecta. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U71081 mRNA Translation: AAB58356.1
U71080 Genomic DNA Translation: AAB58355.1
D78596 mRNA Translation: BAA24355.1
U93215 Genomic DNA Translation: AAB63088.1
CP002685 Genomic DNA Translation: AEC08397.1
AY065145 mRNA Translation: AAL38321.1
BT008875 mRNA Translation: AAP68314.1
PIRiA84709
RefSeqiNP_180607.1, NM_128601.3
UniGeneiAt.23450

Genome annotation databases

EnsemblPlantsiAT2G30490.1; AT2G30490.1; AT2G30490
GeneIDi817599
GrameneiAT2G30490.1; AT2G30490.1; AT2G30490
KEGGiath:AT2G30490

Similar proteinsi

Entry informationi

Entry nameiTCMO_ARATH
AccessioniPrimary (citable) accession number: P92994
Secondary accession number(s): O04995, O49834, P92993
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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