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P92983 (PROD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proline dehydrogenase 1, mitochondrial

EC=1.5.99.8
Alternative name(s):
Osmotic stress-induced proline dehydrogenase
Proline oxidase
Protein EARLY RESPONSIVE TO DEHYDRATION 5
Gene names
Name:POX1
Synonyms:ERD5, PRO1, PRODH1
Ordered Locus Names:At3g30775
ORF Names:MIF6.16, MIF6.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Converts proline to delta-1-pyrroline-5-carboxylate. Ref.8 Ref.11

Catalytic activity

L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.

Cofactor

FAD.

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.

Subcellular location

Mitochondrion Ref.8 Ref.11.

Tissue specificity

Ubiquitous. Highest expression in pollen grains, in the stigma and in developing embryos. Ref.8 Ref.11

Induction

Down-regulated by salt or drought stress. Up-regulated by proline, hypoosmolarity or rehydration. Ref.1 Ref.8 Ref.10 Ref.11

Disruption phenotype

No visible phenotype when grown under normal conditions. Proline hypersensitivity. Ref.9 Ref.11

Sequence similarities

Belongs to the proline oxidase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

CPK4Q388691EBI-2299617,EBI-979475

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Potential
Chain17 – 499483Proline dehydrogenase 1, mitochondrial
PRO_0000025804

Experimental info

Sequence conflict301A → V in AAB40615. Ref.1
Sequence conflict461P → T in AAB40615. Ref.1
Sequence conflict511H → Q in AAB40615. Ref.1
Sequence conflict911A → P in CAA65783. Ref.2
Sequence conflict3551A → R in BAA11682. Ref.3
Sequence conflict3611L → V in CAA65783. Ref.2
Sequence conflict3981V → A in AAL67111. Ref.7
Sequence conflict4251K → R in AAL16138. Ref.7
Sequence conflict4771A → P in AAB40615. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P92983 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 1428611A40EFC824

FASTA49954,956
        10         20         30         40         50         60 
MATRLLRTNF IRRSYRLPAF SPVGPPTVTA STAVVPEILS FGQQAPEPPL HHPKPTEQSH 

        70         80         90        100        110        120 
DGLDLSDQAR LFSSIPTSDL LRSTAVLHAA AIGPMVDLGT WVMSSKLMDA SVTRGMVLGL 

       130        140        150        160        170        180 
VKSTFYDHFC AGEDADAAAE RVRSVYEATG LKGMLVYGVE HADDAVSCDD NMQQFIRTIE 

       190        200        210        220        230        240 
AAKSLPTSHF SSVVVKITAI CPISLLKRVS DLLRWEYKSP NFKLSWKLKS FPVFSESSPL 

       250        260        270        280        290        300 
YHTNSEPEPL TAEEERELEA AHGRIQEICR KCQESNVPLL IDAEDTILQP AIDYMAYSSA 

       310        320        330        340        350        360 
IMFNADKDRP IVYNTIQAYL RDAGERLHLA VQNAEKENVP MGFKLVRGAY MSSEASLADS 

       370        380        390        400        410        420 
LGCKSPVHDT IQDTHSCYND CMTFLMEKAS NGSGFGVVLA THNADSGRLA SRKASDLGID 

       430        440        450        460        470        480 
KQNGKIEFAQ LYGMSDALSF GLKRAGFNVS KYMPFGPVAT AIPYLLRRAY ENRGMMATGA 

       490 
HDRQLMRMEL KRRLIAGIA 

« Hide

References

« Hide 'large scale' references
[1]"Reciprocal regulation of delta 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants."
Peng Z., Lu Q., Verma D.P.S.
Mol. Gen. Genet. 253:334-341(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
Strain: cv. Columbia.
[2]"Environmental and developmental signals modulate proline homeostasis: evidence for a negative transcriptional regulator."
Verbruggen N., Hua X.J., May M., van Montagu M.
Proc. Natl. Acad. Sci. U.S.A. 93:8787-8791(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Root.
[3]"A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis."
Kiyosue T., Yoshiba Y., Yamaguchi-Shinozaki K., Shinozaki K.
Plant Cell 8:1323-1335(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[4]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[5]"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones."
Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.
DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[6]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[7]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[8]"A gene encoding proline dehydrogenase is not only induced by proline and hypoosmolarity, but is also developmentally regulated in the reproductive organs of Arabidopsis."
Nakashima K., Satoh R., Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K.
Plant Physiol. 118:1233-1241(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[9]"Toxicity of free proline revealed in an arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase."
Nanjo T., Fujita M., Seki M., Kato T., Tabata S., Shinozaki K.
Plant Cell Physiol. 44:541-548(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[10]"Two tobacco proline dehydrogenases are differentially regulated and play a role in early plant development."
Ribarits A., Abdullaev A., Tashpulatov A., Richter A., Heberle-Bors E., Touraev A.
Planta 225:1313-1324(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[11]"Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis."
Funck D., Eckard S., Mueller G.
BMC Plant Biol. 10:70-70(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U59508 mRNA. Translation: AAB40615.1.
X97075 mRNA. Translation: CAA65783.1.
D83025 mRNA. Translation: BAA11682.1.
AB028614, AP001296 Genomic DNA. Translation: BAB02917.1.
CP002686 Genomic DNA. Translation: AEE77659.1.
AF428306 mRNA. Translation: AAL16138.1.
AY074571 mRNA. Translation: AAL67111.1.
IPIIPI00526335.
RefSeqNP_189701.3. NM_113981.5.
UniGeneAt.24174.
At.71996.
At.74287.

3D structure databases

ProteinModelPortalP92983.
SMRP92983. Positions 264-488.
ModBaseSearch...

Protein-protein interaction databases

IntActP92983. 1 interaction.
STRING3702.AT3G30775.1-P.

Proteomic databases

PaxDbP92983.
PRIDEP92983.

Protocols and materials databases

DNASU822833.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT3G30775.1; AT3G30775.1; AT3G30775.
GeneID822833.
KEGGath:AT3G30775.

Organism-specific databases

TAIRAt3g30775.

Phylogenomic databases

eggNOGCOG0506.
HOGENOMHOG000243575.
InParanoidP92983.
KOK00318.
OMAGQTGREL.
PhylomeDBP92983.
ProtClustDBPLN02681.

Enzyme and pathway databases

BioCycARA:AT3G30775-MONOMER.
MetaCyc:AT3G30775-MONOMER.
UniPathwayUPA00261; UER00373.

Gene expression databases

GenevestigatorP92983.
GermOnlineAT3G30775. Arabidopsis thaliana.

Family and domain databases

InterProIPR002872. Proline_DH.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERPTHR13914. PTHR13914. 1 hit.
PfamPF01619. Pro_dh. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROD1_ARATH
AccessionPrimary (citable) accession number: P92983
Secondary accession number(s): P92945 expand/collapse secondary AC list , Q8VXV1, Q944L2, Q96281
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families