Proline dehydrogenase 1, mitochondrial precursor - Arabidopsis thaliana (Mouse-ear cress)

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P92983 (PROD1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Proline dehydrogenase 1, mitochondrial
EC=1.5.99.8

Alternative name(s):
Osmotic stress-induced proline dehydrogenase
Proline oxidase
Protein EARLY RESPONSIVE TO DEHYDRATION 5

Gene names

Name:	POX1
Synonyms:	ERD5, PRO1, PRODH1
Ordered Locus Names:	At3g30775
ORF Names:	MIF6.16, MIF6.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts proline to delta-1-pyrroline-5-carboxylate. Ref.8 Ref.11
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Subcellular location	Mitochondrion Ref.8 Ref.11.
Tissue specificity	Ubiquitous. Highest expression in pollen grains, in the stigma and in developing embryos. Ref.8 Ref.11
Induction	Down-regulated by salt or drought stress. Up-regulated by proline, hypoosmolarity or rehydration. Ref.1 Ref.8 Ref.10 Ref.11
Disruption phenotype	No visible phenotype when grown under normal conditions. Proline hypersensitivity. Ref.9 Ref.11
Sequence similarities	Belongs to the proline oxidase family.

Ontologies

Keywords
Biological process	Proline metabolism Stress response
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response to bacterium Inferred from mutant phenotype PubMed 21311034. Source: TAIR glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro proline catabolic process Inferred from genetic interaction Ref.3. Source: TAIR proline catabolic process to glutamate Inferred from electronic annotation. Source: UniProtKB-UniPathway response to oxidative stress Inferred from expression pattern PubMed 9449849. Source: TAIR response to water deprivation Inferred from genetic interaction PubMed 21791601. Source: TAIR
Cellular_component	mitochondrion Inferred from direct assay Ref.3. Source: TAIR
Molecular_function	proline dehydrogenase activity Inferred from mutant phenotype Ref.3. Source: TAIR
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CPK4	Q38869	1	EBI-2299617,EBI-979475

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 16

Mitochondrion Potential

Chain

17 – 499

483

Proline dehydrogenase 1, mitochondrial

PRO_0000025804

Experimental info

Sequence conflict

A → V in AAB40615. Ref.1

Sequence conflict

P → T in AAB40615. Ref.1

Sequence conflict

H → Q in AAB40615. Ref.1

Sequence conflict

A → P in CAA65783. Ref.2

Sequence conflict

355

A → R in BAA11682. Ref.3

Sequence conflict

361

L → V in CAA65783. Ref.2

Sequence conflict

398

V → A in AAL67111. Ref.7

Sequence conflict

425

K → R in AAL16138. Ref.7

Sequence conflict

477

A → P in AAB40615. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P92983 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 1428611A40EFC824
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FASTA

499

54,956

        10         20         30         40         50         60 
MATRLLRTNF IRRSYRLPAF SPVGPPTVTA STAVVPEILS FGQQAPEPPL HHPKPTEQSH 

        70         80         90        100        110        120 
DGLDLSDQAR LFSSIPTSDL LRSTAVLHAA AIGPMVDLGT WVMSSKLMDA SVTRGMVLGL 

       130        140        150        160        170        180 
VKSTFYDHFC AGEDADAAAE RVRSVYEATG LKGMLVYGVE HADDAVSCDD NMQQFIRTIE 

       190        200        210        220        230        240 
AAKSLPTSHF SSVVVKITAI CPISLLKRVS DLLRWEYKSP NFKLSWKLKS FPVFSESSPL 

       250        260        270        280        290        300 
YHTNSEPEPL TAEEERELEA AHGRIQEICR KCQESNVPLL IDAEDTILQP AIDYMAYSSA 

       310        320        330        340        350        360 
IMFNADKDRP IVYNTIQAYL RDAGERLHLA VQNAEKENVP MGFKLVRGAY MSSEASLADS 

       370        380        390        400        410        420 
LGCKSPVHDT IQDTHSCYND CMTFLMEKAS NGSGFGVVLA THNADSGRLA SRKASDLGID 

       430        440        450        460        470        480 
KQNGKIEFAQ LYGMSDALSF GLKRAGFNVS KYMPFGPVAT AIPYLLRRAY ENRGMMATGA 

       490 
HDRQLMRMEL KRRLIAGIA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reciprocal regulation of delta 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants." Peng Z., Lu Q., Verma D.P.S. Mol. Gen. Genet. 253:334-341(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Strain: cv. Columbia.
[2]	"Environmental and developmental signals modulate proline homeostasis: evidence for a negative transcriptional regulator." Verbruggen N., Hua X.J., May M., van Montagu M. Proc. Natl. Acad. Sci. U.S.A. 93:8787-8791(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[3]	"A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis." Kiyosue T., Yoshiba Y., Yamaguchi-Shinozaki K., Shinozaki K. Plant Cell 8:1323-1335(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[5]	"Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC clones." Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S. DNA Res. 7:217-221(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[6]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[7]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[8]	"A gene encoding proline dehydrogenase is not only induced by proline and hypoosmolarity, but is also developmentally regulated in the reproductive organs of Arabidopsis." Nakashima K., Satoh R., Kiyosue T., Yamaguchi-Shinozaki K., Shinozaki K. Plant Physiol. 118:1233-1241(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, INDUCTION.
[9]	"Toxicity of free proline revealed in an arabidopsis T-DNA-tagged mutant deficient in proline dehydrogenase." Nanjo T., Fujita M., Seki M., Kato T., Tabata S., Shinozaki K. Plant Cell Physiol. 44:541-548(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[10]	"Two tobacco proline dehydrogenases are differentially regulated and play a role in early plant development." Ribarits A., Abdullaev A., Tashpulatov A., Richter A., Heberle-Bors E., Touraev A. Planta 225:1313-1324(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[11]	"Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis." Funck D., Eckard S., Mueller G. BMC Plant Biol. 10:70-70(2010) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INDUCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59508 mRNA. Translation: AAB40615.1. X97075 mRNA. Translation: CAA65783.1. D83025 mRNA. Translation: BAA11682.1. AB028614, AP001296 Genomic DNA. Translation: BAB02917.1. CP002686 Genomic DNA. Translation: AEE77659.1. AF428306 mRNA. Translation: AAL16138.1. AY074571 mRNA. Translation: AAL67111.1.
IPI	IPI00526335.
RefSeq	NP_189701.3. NM_113981.5.
UniGene	At.24174. At.71996. At.74287.
3D structure databases
ProteinModelPortal	P92983.
SMR	P92983. Positions 264-488.
ModBase	Search...
Protein-protein interaction databases
IntAct	P92983. 1 interaction.
STRING	3702.AT3G30775.1-P.
Proteomic databases
PaxDb	P92983.
PRIDE	P92983.
Protocols and materials databases
DNASU	822833.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT3G30775.1; AT3G30775.1; AT3G30775.
GeneID	822833.
KEGG	ath:AT3G30775.
Organism-specific databases
TAIR	At3g30775.
Phylogenomic databases
eggNOG	COG0506.
HOGENOM	HOG000243575.
InParanoid	P92983.
KO	K00318.
OMA	GQTGREL.
PhylomeDB	P92983.
ProtClustDB	PLN02681.
Enzyme and pathway databases
BioCyc	ARA:AT3G30775-MONOMER. MetaCyc:AT3G30775-MONOMER.
UniPathway	UPA00261; UER00373.
Gene expression databases
Genevestigator	P92983.
GermOnline	AT3G30775. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002872. Proline_DH. IPR015659. Proline_oxidase. [Graphical view]
PANTHER	PTHR13914. PTHR13914. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROD1_ARATH

Accession

Primary (citable) accession number: P92983
Secondary accession number(s): P92945

Q96281

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents