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Reviewed, UniProtKB/Swiss-Prot P92983 (PROD_ARATH)

Last modified November 25, 2008. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Proline oxidase, mitochondrial
    EC=1.5.99.8
Alternative name(s):
    Osmotic stress-induced proline dehydrogenase
Gene names
Name: POX
Synonyms: ERD5, PRO1
Ordered Locus Names: At3g30775
ORF Names: MIF6.19
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Converts proline to delta-1-pyrroline-5-carboxylate.

Catalytic activity

L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.

Cofactor

FAD.

Pathway

Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.

Subcellular location

Mitochondrion matrix.

Induction

By osmotic stress.

Sequence similarities

Belongs to the proline oxidase family.

Ontologies

Keywords

   Biological processProline metabolism
Stress response
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandFAD
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processglutamate biosynthetic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

proline catabolic process

Inferred from electronic annotation. Source: InterPro

response to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionproline dehydrogenase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 1616Mitochondrion Potential
Chain17 – 499483Proline oxidase, mitochondrial
PRO_0000025804

Experimental info

Sequence conflict301A → V in AAB40615. Ref.1
Sequence conflict461P → T in AAB40615. Ref.1
Sequence conflict511H → Q in AAB40615. Ref.1
Sequence conflict911A → P in CAA65783. Ref.2
Sequence conflict3551A → R in BAA11682. Ref.3
Sequence conflict3611L → V in CAA65783. Ref.2
Sequence conflict4771A → P in AAB40615. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P92983-1 [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: 1428611A40EFC824

FASTA49954,956
        10         20         30         40         50         60 
MATRLLRTNF IRRSYRLPAF SPVGPPTVTA STAVVPEILS FGQQAPEPPL HHPKPTEQSH 

        70         80         90        100        110        120 
DGLDLSDQAR LFSSIPTSDL LRSTAVLHAA AIGPMVDLGT WVMSSKLMDA SVTRGMVLGL 

       130        140        150        160        170        180 
VKSTFYDHFC AGEDADAAAE RVRSVYEATG LKGMLVYGVE HADDAVSCDD NMQQFIRTIE 

       190        200        210        220        230        240 
AAKSLPTSHF SSVVVKITAI CPISLLKRVS DLLRWEYKSP NFKLSWKLKS FPVFSESSPL 

       250        260        270        280        290        300 
YHTNSEPEPL TAEEERELEA AHGRIQEICR KCQESNVPLL IDAEDTILQP AIDYMAYSSA 

       310        320        330        340        350        360 
IMFNADKDRP IVYNTIQAYL RDAGERLHLA VQNAEKENVP MGFKLVRGAY MSSEASLADS 

       370        380        390        400        410        420 
LGCKSPVHDT IQDTHSCYND CMTFLMEKAS NGSGFGVVLA THNADSGRLA SRKASDLGID 

       430        440        450        460        470        480 
KQNGKIEFAQ LYGMSDALSF GLKRAGFNVS KYMPFGPVAT AIPYLLRRAY ENRGMMATGA 

       490 
HDRQLMRMEL KRRLIAGIA 

« Hide

References

« Hide 'large scale' references
[1]"Reciprocal regulation of delta 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants."
Peng Z., Lu Q., Verma D.P.S.
Mol. Gen. Genet. 253:334-341(1996) [PubMed: 9003320] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Environmental and developmental signals modulate proline homeostasis: evidence for a negative transcriptional regulator."
Verbruggen N., Hua X.J., May M., van Montagu M.
Proc. Natl. Acad. Sci. U.S.A. 93:8787-8791(1996) [PubMed: 8710950] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
Tissue: Root.
[3]"A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis."
Kiyosue T., Yoshiba Y., Yamaguchi-Shinozaki K., Shinozaki K.
Plant Cell 8:1323-1335(1996) [PubMed: 8776899] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[4]"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones."
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.
DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.

Cross-references

Sequence databases

U59508 mRNA. Translation: AAB40615.1.
X97075 mRNA. Translation: CAA65783.1.
D83025 mRNA. Translation: BAA11682.1.
AB028614, AP001296 Genomic DNA. Translation: BAB02917.1.
RefSeqNP_189701.3.
UniGeneAt.24174

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID822833.
GenomeReviewsGene locus AT3G30775 in contig BA000014_GR.
KEGGath:AT3G30775.
NMPDRfig|3702.1.peg.15354.

Organism-specific databases

TAIRAt3g30775.

Gene expression databases

ArrayExpressP92983.
GermOnlineAT3G30775. Arabidopsis thaliana.

Family and domain databases

InterProIPR002872. Proline_DHase.
IPR015659. Proline_oxidase.
[Graphical view]
PANTHERPTHR13914. Proline_oxidase. 1 hit.
PfamPF01619. Pro_dh. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePROD_ARATH
AccessionPrimary (citable) accession number: P92983
Secondary accession number(s): P92945, Q96281
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 25, 2008
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents