Reviewed, UniProtKB/Swiss-Prot P92983 (PROD_ARATH)

Names and origin

Protein names

Recommended name:
    Proline dehydrogenase, mitochondrial
    EC=1.5.99.8
Alternative name(s):
    Proline oxidase
    Osmotic stress-induced proline dehydrogenase

Gene names

Name:	POX
Synonyms:	ERD5, PRO1
Ordered Locus Names:	At3g30775
ORF Names:	MIF6.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts proline to delta-1-pyrroline-5-carboxylate.
Catalytic activity	L-proline + acceptor = (S)-1-pyrroline-5-carboxylate + reduced acceptor.
Cofactor	FAD.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 1/2.
Subcellular location	Mitochondrion matrix.
Induction	By osmotic stress.
Sequence similarities	Belongs to the proline oxidase family.

Ontologies

Keywords
Biological process	Proline metabolism Stress response
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline catabolic process Ref.3 Inferred from genetic interaction. Source: TAIR response to oxidative stress Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	proline dehydrogenase activity Ref.3 Inferred from mutant phenotype. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CPK4	Q38869	1	EBI-2299617,EBI-979475

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 16	16	Mitochondrion Potential
	Chain	17 – 499	483	Proline dehydrogenase, mitochondrial		PRO_0000025804
Experimental info
	Sequence conflict	30	1	A → V in AAB40615. Ref.1
	Sequence conflict	46	1	P → T in AAB40615. Ref.1
	Sequence conflict	51	1	H → Q in AAB40615. Ref.1
	Sequence conflict	91	1	A → P in CAA65783. Ref.2
	Sequence conflict	355	1	A → R in BAA11682. Ref.3
	Sequence conflict	361	1	L → V in CAA65783. Ref.2
	Sequence conflict	477	1	A → P in AAB40615. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P92983-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 1428611A40EFC824	FASTA	499	54,956
	10 20 30 40 50 60 MATRLLRTNF IRRSYRLPAF SPVGPPTVTA STAVVPEILS FGQQAPEPPL HHPKPTEQSH 70 80 90 100 110 120 DGLDLSDQAR LFSSIPTSDL LRSTAVLHAA AIGPMVDLGT WVMSSKLMDA SVTRGMVLGL 130 140 150 160 170 180 VKSTFYDHFC AGEDADAAAE RVRSVYEATG LKGMLVYGVE HADDAVSCDD NMQQFIRTIE 190 200 210 220 230 240 AAKSLPTSHF SSVVVKITAI CPISLLKRVS DLLRWEYKSP NFKLSWKLKS FPVFSESSPL 250 260 270 280 290 300 YHTNSEPEPL TAEEERELEA AHGRIQEICR KCQESNVPLL IDAEDTILQP AIDYMAYSSA 310 320 330 340 350 360 IMFNADKDRP IVYNTIQAYL RDAGERLHLA VQNAEKENVP MGFKLVRGAY MSSEASLADS 370 380 390 400 410 420 LGCKSPVHDT IQDTHSCYND CMTFLMEKAS NGSGFGVVLA THNADSGRLA SRKASDLGID 430 440 450 460 470 480 KQNGKIEFAQ LYGMSDALSF GLKRAGFNVS KYMPFGPVAT AIPYLLRRAY ENRGMMATGA 490 HDRQLMRMEL KRRLIAGIA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reciprocal regulation of delta 1-pyrroline-5-carboxylate synthetase and proline dehydrogenase genes controls proline levels during and after osmotic stress in plants." Peng Z., Lu Q., Verma D.P.S. Mol. Gen. Genet. 253:334-341(1996) [PubMed: 9003320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Environmental and developmental signals modulate proline homeostasis: evidence for a negative transcriptional regulator." Verbruggen N., Hua X.J., May M., van Montagu M. Proc. Natl. Acad. Sci. U.S.A. 93:8787-8791(1996) [PubMed: 8710950] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. Tissue: Root.
[3]	"A nuclear gene encoding mitochondrial proline dehydrogenase, an enzyme involved in proline metabolism, is upregulated by proline but downregulated by dehydration in Arabidopsis." Kiyosue T., Yoshiba Y., Yamaguchi-Shinozaki K., Shinozaki K. Plant Cell 8:1323-1335(1996) [PubMed: 8776899] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[4]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U59508 mRNA. Translation: AAB40615.1. X97075 mRNA. Translation: CAA65783.1. D83025 mRNA. Translation: BAA11682.1. AB028614, AP001296 Genomic DNA. Translation: BAB02917.1.
IPI	IPI00526335.
RefSeq	NP_189701.3.
UniGene	At.24174 At.71996
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	P92983.
Genome annotation databases
GeneID	822833.
GenomeReviews	Gene locus AT3G30775 in contig BA000014_GR.
KEGG	ath:AT3G30775.
NMPDR	fig|3702.1.peg.15354.
Organism-specific databases
TAIR	At3g30775.
Phylogenomic databases
OMA	P92983. LATHNAD.
Enzyme and pathway databases
BRENDA	1.5.99.8. 302.
Gene expression databases
ArrayExpress	P92983.
GermOnline	AT3G30775. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002872. Proline_DH. IPR015659. Proline_oxidase. [Graphical view]
PANTHER	PTHR13914. Proline_oxidase. 1 hit.
Pfam	PF01619. Pro_dh. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROD_ARATH

Accession

Primary (citable) accession number: P92983
Secondary accession number(s): P92945, Q96281

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)