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Protein

5'-adenylylsulfate reductase 2, chloroplastic

Gene

APR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons.1 Publication

Catalytic activityi

AMP + sulfite + glutathione disulfide = adenylyl sulfate + 2 glutathione.

Cofactori

[4Fe-4S] cluster1 PublicationNote: Binds 1 [4Fe-4S] cluster.1 Publication

Enzyme regulationi

Stimulated by sodium sulfate > ammonium sulfate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei374NucleophileBy similarity1
Active sitei377NucleophileBy similarity1

GO - Molecular functioni

  • adenylyl-sulfate reductase (glutathione) activity Source: UniProtKB-EC
  • adenylyl-sulfate reductase activity Source: TAIR
  • phosphoadenylyl-sulfate reductase (thioredoxin) activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Stress response

Enzyme and pathway databases

BioCyciMetaCyc:AT1G62180-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
5'-adenylylsulfate reductase 2, chloroplastic (EC:1.8.4.9)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate reductase homolog 43
Short name:
PAPS reductase homolog 43
Short name:
Prh-43
Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 2
Short name:
APS sulfotransferase 2
Thioredoxin-independent APS reductase 2
Gene namesi
Name:APR2
Synonyms:APSR, PRH43
Ordered Locus Names:At1g62180
ORF Names:F19K23.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G62180
TAIRilocus:2018097 AT1G62180

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi195C → S: Slightly reduces activity. 1 Publication1
Mutagenesisi314C → S: Abolishes activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 66ChloroplastSequence analysisCombined sourcesAdd BLAST66
ChainiPRO_000002321567 – 4545'-adenylylsulfate reductase 2, chloroplasticAdd BLAST388

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67N-acetylalanineCombined sources1
Disulfide bondi374 ↔ 377Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP92981
PRIDEiP92981

PTM databases

iPTMnetiP92981

Expressioni

Tissue specificityi

Leaves and stem.

Inductioni

By sulfate starvation.

Gene expression databases

ExpressionAtlasiP92981 baseline and differential
GenevisibleiP92981 AT

Interactioni

Protein-protein interaction databases

IntActiP92981 1 interactor.
STRINGi3702.AT1G62180.1

Structurei

3D structure databases

ProteinModelPortaliP92981
SMRiP92981
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini333 – 454ThioredoxinPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 319Reductase domainAdd BLAST253

Domaini

The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase (By similarity).By similarity

Sequence similaritiesi

Belongs to the APS reductase family.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0189 Eukaryota
KOG0191 Eukaryota
COG0175 LUCA
HOGENOMiHOG000242337
KOiK05907
OMAiMVVDTFH
OrthoDBiEOG093609DB
PhylomeDBiP92981

Family and domain databases

CDDicd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.6201 hit
InterProiView protein in InterPro
IPR002500 PAPS_reduct
IPR014729 Rossmann-like_a/b/a_fold
IPR004508 Thioredoxin-indep_APS_Rdtase
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF01507 PAPS_reduct, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR00424 APS_reduc, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P92981-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALAVTSSST AISGSSFSRS GASSESKALQ ICSIRLSDRT HLSQRRYSMK
60 70 80 90 100
PLNAESHSRS ESWVTRASTL IAPEVEEKGG EVEDFEQLAK KLEDASPLEI
110 120 130 140 150
MDKALERFGD QIAIAFSGAE DVALIEYARL TGKPFRVFSL DTGRLNPETY
160 170 180 190 200
RLFDAVEKQY GIRIEYMFPD AVEVQALVRN KGLFSFYEDG HQECCRVRKV
210 220 230 240 250
RPLRRALKGL KAWITGQRKD QSPGTRSEIP IVQVDPVFEG LDGGVGSLVK
260 270 280 290 300
WNPLANVEGA DVWNFLRTMD VPVNALHAQG YVSIGCEPCT RPVLPGQHER
310 320 330 340 350
EGRWWWEDAK AKECGLHKGN IKEEDGAADS KPAAVQEIFE SNNVVALSKG
360 370 380 390 400
GVENLLKLEN RKEAWLVVLY APWCPFCQAM EASYIELAEK LAGKGVKVAK
410 420 430 440 450
FRADGEQKEF AKQELQLGSF PTILLFPKRA PRAIKYPSEH RDVDSLMSFV

NLLR
Length:454
Mass (Da):50,656
Last modified:October 19, 2002 - v2
Checksum:i4EB93C1FFC5E4636
GO

Sequence cautioni

The sequence AAC26977 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16S → T in AAB57688 (Ref. 3) Curated1
Sequence conflicti16S → T in AAM66987 (Ref. 8) Curated1
Sequence conflicti40T → N in AAB57688 (Ref. 3) Curated1
Sequence conflicti40T → N in AAM66987 (Ref. 8) Curated1
Sequence conflicti43S → A in AAC49563 (PubMed:8917599).Curated1
Sequence conflicti47 – 48YS → P in AAC49563 (PubMed:8917599).Curated2
Sequence conflicti57 – 58HS → SHT in AAB57688 (Ref. 3) Curated2
Sequence conflicti57 – 58HS → SHT in AAM66987 (Ref. 8) Curated2
Sequence conflicti65T → L in AAB57688 (Ref. 3) Curated1
Sequence conflicti65T → L in AAM66987 (Ref. 8) Curated1
Sequence conflicti79G → E in AAB57688 (Ref. 3) Curated1
Sequence conflicti79G → E in AAM66987 (Ref. 8) Curated1
Sequence conflicti107R → K in AAB57688 (Ref. 3) Curated1
Sequence conflicti107R → K in AAM66987 (Ref. 8) Curated1
Sequence conflicti111Q → E in AAB57688 (Ref. 3) Curated1
Sequence conflicti111Q → E in AAM66987 (Ref. 8) Curated1
Sequence conflicti291R → S in AAC26977 (PubMed:8917600).Curated1
Sequence conflicti322K → F in AAC26977 (PubMed:8917600).Curated1
Sequence conflicti349K → R in AAB57688 (Ref. 3) Curated1
Sequence conflicti349K → R in AAM66987 (Ref. 8) Curated1
Sequence conflicti351G → R in AAC26977 (PubMed:8917600).Curated1
Sequence conflicti385I → V in AAB57688 (Ref. 3) Curated1
Sequence conflicti385I → V in AAM66987 (Ref. 8) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53866 mRNA Translation: AAC49563.1
AF023167 mRNA Translation: AAB80957.1
U96045 mRNA Translation: AAB57688.1
AF016283 Genomic DNA Translation: AAC26980.1
AC000375 Genomic DNA Translation: AAB60764.1
CP002684 Genomic DNA Translation: AEE33931.1
AF360192 mRNA Translation: AAK25902.1
AY040005 mRNA Translation: AAK64082.1
AY088665 mRNA Translation: AAM66987.1
U56921 mRNA Translation: AAC26977.1 Different initiation.
PIRiC96648
RefSeqiNP_176409.1, NM_104899.3 [P92981-1]
UniGeneiAt.25368
At.74995

Genome annotation databases

EnsemblPlantsiAT1G62180.1; AT1G62180.1; AT1G62180 [P92981-1]
GeneIDi842514
GrameneiAT1G62180.1; AT1G62180.1; AT1G62180 [P92981-1]
KEGGiath:AT1G62180

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiAPR2_ARATH
AccessioniPrimary (citable) accession number: P92981
Secondary accession number(s): O04215
, O04583, O22554, Q38947, Q541D4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: April 25, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome