P92980 (APR3_ARATH) Reviewed, UniProtKB/Swiss-Prot
Last modified
December 14, 2011.
Version 110.
History...
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: 5'-adenylylsulfate reductase 3, chloroplastic EC=1.8.4.9 Alternative name(s): 3'-phosphoadenosine-5'-phosphosulfate reductase homolog 26 Short name=PAPS reductase homolog 26 Short name=Prh-26 Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 3 Short name=APS sulfotransferase 3 Thioredoxin-independent APS reductase 3 | ||||||||
| Gene names |
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| Organism | Arabidopsis thaliana (Mouse-ear cress) | ||||||||
| Taxonomic identifier | 3702 [NCBI] | ||||||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Protein attributes
| Sequence length | 458 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons. |
| Catalytic activity | AMP + sulfite + glutathione disulfide = adenylyl sulfate + 2 glutathione. |
| Cofactor | Binds 1 4Fe-4S cluster By similarity. |
| Enzyme regulation | Stimulated by sodium sulfate > ammonium sulfate By similarity. |
| Subcellular location | Plastid › chloroplast Potential. |
| Tissue specificity | Leaves, roots and stem. |
| Induction | By sulfate starvation. |
| Domain | The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase By similarity. |
| Sequence similarities | Belongs to the APS reductase family. Contains 1 thioredoxin domain. |
| Sequence caution | The sequence AAC26978.1 differs from that shown. Reason: Frameshift at position 409. |
Ontologies
Alternative products
| This entry describes 1 isoform produced by alternative splicing. [Select] Note: A number of isoforms are produced. According to EST sequences. | ||||||
| Isoform 1 (identifier: P92980-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||
Molecule processing | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 69 | 69 | Chloroplast Potential | ||||||||
| Chain | 70 – 458 | 389 | 5'-adenylylsulfate reductase 3, chloroplastic | PRO_0000023216 | |||||||
Regions | |||||||||||
| Domain | 337 – 458 | 122 | Thioredoxin | ||||||||
| Region | 70 – 319 | 250 | Reductase domain | ||||||||
| Compositional bias | 8 – 54 | 47 | Ser-rich | ||||||||
Sites | |||||||||||
| Active site | 378 | 1 | Nucleophile By similarity | ||||||||
| Active site | 381 | 1 | Nucleophile By similarity | ||||||||
Amino acid modifications | |||||||||||
| Disulfide bond | 378 ↔ 381 | Redox-active By similarity | |||||||||
Experimental info | |||||||||||
| Sequence conflict | 28 | 1 | T → A in AAC26978. Ref.2 | ||||||||
| Sequence conflict | 121 | 1 | D → Y in AAC26978. Ref.2 | ||||||||
| Sequence conflict | 264 | 1 | N → D in BAH57002. Ref.7 | ||||||||
| Sequence conflict | 338 | 1 | S → T in AAM65133. Ref.8 | ||||||||
| Sequence conflict | 375 | 1 | A → R in AAC49562. Ref.1 | ||||||||
| Sequence conflict | 436 | 1 | R → I in AAC49562. Ref.1 | ||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Three members of a novel small gene-family from Arabidopsis thaliana able to complement functionally an Escherichia coli mutant defective in PAPS reductase activity encode proteins with a thioredoxin-like domain and 'APS reductase' activity." Gutierrez-Marcos J.F., Roberts M.A., Campbell E.I., Wray J.L. Proc. Natl. Acad. Sci. U.S.A. 93:13377-13382(1996) [PubMed: 8917599] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia. |
| [2] | "Sulfate reduction in higher plants: molecular evidence for a novel 5'-adenylylsulfate reductase." Setya A., Murillo M., Leustek T. Proc. Natl. Acad. Sci. U.S.A. 93:13383-13388(1996) [PubMed: 8917600] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: cv. Columbia. |
| [3] | "Three genomic clones from Arabidopisis thaliana encoding 5'-adenylysulfate reductase." Chen Y.C., Leustek T. Plant Gene Register PGR98-030 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. |
| [4] | "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. McCombie W.R.Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia. |
| [5] | The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia. |
| [6] | "Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R.Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. |
| [7] | "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed: 19423640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia. Tissue: Rosette leaf. |
| [8] | "Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U53865 mRNA. Translation: AAC49562.1. U56922 mRNA. Translation: AAC26978.1. Frameshift. AF016284 Genomic DNA. Translation: AAC26981.1. AL022140 Genomic DNA. Translation: CAA18102.1. AL161556 Genomic DNA. Translation: CAB79154.1. CP002687 Genomic DNA. Translation: AEE84539.1. AF428445 mRNA. Translation: AAL16214.1. AY054175 mRNA. Translation: AAL06836.1. AY062665 mRNA. Translation: AAL32743.1. AY093319 mRNA. Translation: AAM13318.1. AY103313 mRNA. Translation: AAM65364.1. AK318887 mRNA. Translation: BAH57002.1. AY087591 mRNA. Translation: AAM65133.1. |
| IPI | IPI00545488. |
| PIR | T49106. |
| RefSeq | NP_193930.1. NM_118320.3. |
| UniGene | At.2106. At.24034. |
3D structure databases | |
| ProteinModelPortal | P92980. |
| SMR | P92980. Positions 83-307, 345-456. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P92980. 2 interactions. |
| STRING | P92980. |
Proteomic databases | |
| PRIDE | P92980. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| EnsemblPlants | AT4G21990.1; AT4G21990.1; AT4G21990. |
| GeneID | 828288. |
| GenomeReviews | Gene locus AT4G21990 in contig CT486007_GR. |
| KEGG | ath:AT4G21990. |
| NMPDR | fig|3702.1.peg.20048. |
Organism-specific databases | |
| TAIR | At4g21990. |
Phylogenomic databases | |
| eggNOG | KOG0189. |
| HOGENOM | HBG758022. |
| InParanoid | P92980. |
| OMA | QECCRIR. |
| PhylomeDB | P92980. |
| ProtClustDB | PLN02309. |
Gene expression databases | |
| ArrayExpress | P92980. |
| Genevestigator | P92980. |
| GermOnline | AT4G21990. Arabidopsis thaliana. |
Family and domain databases | |
| InterPro | IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. IPR004508. Thioredoxin-indep_APS_Rdtase. IPR012336. Thioredoxin-like_fold. IPR013766. Thioredoxin_domain. [Graphical view] |
| Gene3D | G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit. |
| KO | K05907. |
| Pfam | PF01507. PAPS_reduct. 1 hit. PF00085. Thioredoxin. 1 hit. [Graphical view] |
| SUPFAM | SSF52833. Thiordxn-like_fd. 1 hit. |
| TIGRFAMs | TIGR00424. APS_reduc. 1 hit. |
| PROSITE | PS00194. THIOREDOXIN_1. False negative. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | APR3_ARATH | ||||||||
| Accession | Primary (citable) accession number: P92980 Secondary accession number(s): C0Z2S3 Q8LAV2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| Arabidopsis thaliana Arabidopsis thaliana: entries and gene names |
| SIMILARITY comments Index of protein domains and families |

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