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Protein

5'-adenylylsulfate reductase 3, chloroplastic

Gene

APR3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons.

Catalytic activityi

AMP + sulfite + glutathione disulfide = adenylyl sulfate + 2 glutathione.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Enzyme regulationi

Stimulated by sodium sulfate > ammonium sulfate.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei378NucleophileBy similarity1
Active sitei381NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Stress response

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT4G21990-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
5'-adenylylsulfate reductase 3, chloroplastic (EC:1.8.4.9)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate reductase homolog 26
Short name:
PAPS reductase homolog 26
Short name:
Prh-26
Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 3
Short name:
APS sulfotransferase 3
Thioredoxin-independent APS reductase 3
Gene namesi
Name:APR3
Synonyms:PRH26
Ordered Locus Names:At4g21990
ORF Names:F1N20.90
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G21990.

Subcellular locationi

GO - Cellular componenti

  • chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 69ChloroplastSequence analysisAdd BLAST69
ChainiPRO_000002321670 – 4585'-adenylylsulfate reductase 3, chloroplasticAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi378 ↔ 381Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP92980.

Expressioni

Tissue specificityi

Leaves, roots and stem.

Inductioni

By sulfate starvation.

Gene expression databases

ExpressionAtlasiP92980. baseline and differential.
GenevisibleiP92980. AT.

Interactioni

Protein-protein interaction databases

BioGridi13577. 1 interactor.
IntActiP92980. 2 interactors.
STRINGi3702.AT4G21990.1.

Structurei

3D structure databases

ProteinModelPortaliP92980.
SMRiP92980.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini337 – 458ThioredoxinPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni70 – 319Reductase domainAdd BLAST250

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 54Ser-richAdd BLAST47

Domaini

The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase (By similarity).By similarity

Sequence similaritiesi

Belongs to the APS reductase family.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0189. Eukaryota.
KOG0191. Eukaryota.
COG0175. LUCA.
HOGENOMiHOG000242337.
KOiK05907.
OrthoDBiEOG093609DB.
PhylomeDBiP92980.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004508. Thioredoxin-indep_APS_Rdtase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00424. APS_reduc. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P92980-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALAINVSSS SSSAISSSSF PSSDLKVTKI GSLRLLNRTN VSAASLSLSG
60 70 80 90 100
KRSSVKALNV QSITKESIVA SEVTEKLDVV EVEDFEELAK RLENASPLEI
110 120 130 140 150
MDKALEKFGN DIAIAFSGAE DVALIEYAHL TGRPYRVFSL DTGRLNPETY
160 170 180 190 200
RLFDTVEKHY GIRIEYMFPD AVEVQALVRN KGLFSFYEDG HQECCRIRKV
210 220 230 240 250
RPLRRALKGL RAWITGQRKD QSPGTRSEIP VVQVDPVFEG LDGGVGSLVK
260 270 280 290 300
WNPVANVEGN DVWNFLRTMD VPVNTLHAAG YVSIGCEPCT RAVLPGQHER
310 320 330 340 350
EGRWWWEDAK AKECGLHKGN IKENTNGNAT ANVNGTASVA DIFNSENVVN
360 370 380 390 400
LSRQGIENLM KLENRKEAWI VVLYAPWCPF CQAMEASFDE LADKLGGSGV
410 420 430 440 450
KVAKFRADGD QKDFAKKELQ LGSFPTILVF PKNSSRPIKY PSEKRDVDSL

TSFLNLVR
Length:458
Mass (Da):50,695
Last modified:October 19, 2002 - v2
Checksum:iE950C28E15F418CB
GO

Sequence cautioni

The sequence AAC26978 differs from that shown. Reason: Frameshift at position 409.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28T → A in AAC26978 (PubMed:8917600).Curated1
Sequence conflicti121D → Y in AAC26978 (PubMed:8917600).Curated1
Sequence conflicti264N → D in BAH57002 (PubMed:19423640).Curated1
Sequence conflicti338S → T in AAM65133 (Ref. 8) Curated1
Sequence conflicti375A → R in AAC49562 (PubMed:8917599).Curated1
Sequence conflicti436R → I in AAC49562 (PubMed:8917599).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53865 mRNA. Translation: AAC49562.1.
U56922 mRNA. Translation: AAC26978.1. Frameshift.
AF016284 Genomic DNA. Translation: AAC26981.1.
AL022140 Genomic DNA. Translation: CAA18102.1.
AL161556 Genomic DNA. Translation: CAB79154.1.
CP002687 Genomic DNA. Translation: AEE84539.1.
AF428445 mRNA. Translation: AAL16214.1.
AY054175 mRNA. Translation: AAL06836.1.
AY062665 mRNA. Translation: AAL32743.1.
AY093319 mRNA. Translation: AAM13318.1.
AY103313 mRNA. Translation: AAM65364.1.
AK318887 mRNA. Translation: BAH57002.1.
AY087591 mRNA. Translation: AAM65133.1.
PIRiT49106.
RefSeqiNP_193930.1. NM_118320.3. [P92980-1]
UniGeneiAt.2106.
At.24034.

Genome annotation databases

EnsemblPlantsiAT4G21990.1; AT4G21990.1; AT4G21990. [P92980-1]
GeneIDi828288.
GrameneiAT4G21990.1; AT4G21990.1; AT4G21990.
KEGGiath:AT4G21990.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53865 mRNA. Translation: AAC49562.1.
U56922 mRNA. Translation: AAC26978.1. Frameshift.
AF016284 Genomic DNA. Translation: AAC26981.1.
AL022140 Genomic DNA. Translation: CAA18102.1.
AL161556 Genomic DNA. Translation: CAB79154.1.
CP002687 Genomic DNA. Translation: AEE84539.1.
AF428445 mRNA. Translation: AAL16214.1.
AY054175 mRNA. Translation: AAL06836.1.
AY062665 mRNA. Translation: AAL32743.1.
AY093319 mRNA. Translation: AAM13318.1.
AY103313 mRNA. Translation: AAM65364.1.
AK318887 mRNA. Translation: BAH57002.1.
AY087591 mRNA. Translation: AAM65133.1.
PIRiT49106.
RefSeqiNP_193930.1. NM_118320.3. [P92980-1]
UniGeneiAt.2106.
At.24034.

3D structure databases

ProteinModelPortaliP92980.
SMRiP92980.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi13577. 1 interactor.
IntActiP92980. 2 interactors.
STRINGi3702.AT4G21990.1.

Proteomic databases

PaxDbiP92980.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G21990.1; AT4G21990.1; AT4G21990. [P92980-1]
GeneIDi828288.
GrameneiAT4G21990.1; AT4G21990.1; AT4G21990.
KEGGiath:AT4G21990.

Organism-specific databases

TAIRiAT4G21990.

Phylogenomic databases

eggNOGiKOG0189. Eukaryota.
KOG0191. Eukaryota.
COG0175. LUCA.
HOGENOMiHOG000242337.
KOiK05907.
OrthoDBiEOG093609DB.
PhylomeDBiP92980.

Enzyme and pathway databases

BioCyciMetaCyc:AT4G21990-MONOMER.

Miscellaneous databases

PROiP92980.

Gene expression databases

ExpressionAtlasiP92980. baseline and differential.
GenevisibleiP92980. AT.

Family and domain databases

CDDicd01713. PAPS_reductase. 1 hit.
Gene3Di3.40.30.10. 1 hit.
3.40.50.620. 1 hit.
InterProiIPR002500. PAPS_reduct.
IPR014729. Rossmann-like_a/b/a_fold.
IPR004508. Thioredoxin-indep_APS_Rdtase.
IPR012336. Thioredoxin-like_fold.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF01507. PAPS_reduct. 1 hit.
PF00085. Thioredoxin. 1 hit.
[Graphical view]
SUPFAMiSSF52833. SSF52833. 1 hit.
TIGRFAMsiTIGR00424. APS_reduc. 1 hit.
PROSITEiPS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAPR3_ARATH
AccessioniPrimary (citable) accession number: P92980
Secondary accession number(s): C0Z2S3
, O48887, Q38948, Q8LAV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: November 30, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.