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Protein

5'-adenylylsulfate reductase 1, chloroplastic

Gene

APR1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Reduces sulfate for Cys biosynthesis. Substrate preference is adenosine-5'-phosphosulfate (APS) >> 3'-phosphoadenosine-5'-phosphosulfate (PAPS). Uses glutathione or DTT as source of protons.

Catalytic activityi

AMP + sulfite + glutathione disulfide = adenylyl sulfate + 2 glutathione.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster.By similarity

Enzyme regulationi

Stimulated by sodium sulfate > ammonium sulfate and is sensitive to inactivation by 5'AMP.

pH dependencei

Optimum pH is 8.5.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei385NucleophileBy similarity1
Active sitei388NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • cysteine biosynthetic process Source: UniProtKB-KW
  • sulfate assimilation Source: TAIR
  • sulfate reduction Source: TAIR

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Stress response
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:AT4G04610-MONOMER
BRENDAi1.8.4.9 399

Names & Taxonomyi

Protein namesi
Recommended name:
5'-adenylylsulfate reductase 1, chloroplastic (EC:1.8.4.9)
Alternative name(s):
3'-phosphoadenosine-5'-phosphosulfate reductase homolog 19
Short name:
PAPS reductase homolog 19
Short name:
Prh-19
Adenosine 5'-phosphosulfate 5'-adenylylsulfate sulfotransferase 1
Short name:
APS sulfotransferase 1
Thioredoxin-independent APS reductase 1
Gene namesi
Name:APR1
Synonyms:PRH19
Ordered Locus Names:At4g04610
ORF Names:F4H6.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G04610
TAIRilocus:2125786 AT4G04610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53ChloroplastSequence analysisAdd BLAST53
ChainiPRO_000002321454 – 4655'-adenylylsulfate reductase 1, chloroplasticAdd BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi385 ↔ 388Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP92979

Expressioni

Tissue specificityi

Leaves, roots and stem.

Inductioni

By sulfate starvation.

Gene expression databases

ExpressionAtlasiP92979 baseline and differential
GenevisibleiP92979 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G04610.1

Structurei

3D structure databases

ProteinModelPortaliP92979
SMRiP92979
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini344 – 465ThioredoxinPROSITE-ProRule annotationAdd BLAST122

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni73 – 327Reductase domainAdd BLAST255

Domaini

The C-terminal domain may function as glutaredoxin and mediates the interaction of the enzyme with glutathione (GSH). Active in GSH-dependent reduction of hydroxyethyldisulfide, cystine, dehydroascorbate, insulin disulfides and ribonucleotide reductase.

Sequence similaritiesi

Belongs to the APS reductase family.Curated

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0189 Eukaryota
KOG0191 Eukaryota
COG0175 LUCA
HOGENOMiHOG000242337
KOiK05907
OMAiMAMSVNV
OrthoDBiEOG093609DB
PhylomeDBiP92979

Family and domain databases

CDDicd01713 PAPS_reductase, 1 hit
Gene3Di3.40.50.620, 1 hit
InterProiView protein in InterPro
IPR002500 PAPS_reduct
IPR014729 Rossmann-like_a/b/a_fold
IPR004508 Thioredoxin-indep_APS_Rdtase
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF01507 PAPS_reduct, 1 hit
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR00424 APS_reduc, 1 hit
PROSITEiView protein in PROSITE
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P92979-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMSVNVSSS SSSGIINSRF GVSLEPKVSQ IGSLRLLDRV HVAPVSLNLS
60 70 80 90 100
GKRSSSVKPL NAEPKTKDSM IPLAATMVAE IAEEVEVVEI EDFEELAKKL
110 120 130 140 150
ENASPLEIMD KALEKYGNDI AIAFSGAEDV ALIEYAHLTG RPFRVFSLDT
160 170 180 190 200
GRLNPETYRF FDAVEKHYGI RIEYMFPDSV EVQGLVRSKG LFSFYEDGHQ
210 220 230 240 250
ECCRVRKVRP LRRALKGLKA WITGQRKDQS PGTRSEIPVV QVDPVFEGLD
260 270 280 290 300
GGVGSLVKWN PVANVEGNDV WNFLRTMDVP VNTLHAAGYI SIGCEPCTKA
310 320 330 340 350
VLPGQHEREG RWWWEDAKAK ECGLHKGNVK ENSDDAKVNG ESKSAVADIF
360 370 380 390 400
KSENLVTLSR QGIENLMKLE NRKEPWIVVL YAPWCPFCQA MEASYDELAD
410 420 430 440 450
KLAGSGIKVA KFRADGDQKE FAKQELQLGS FPTILVFPKN SSRPIKYPSE
460
KRDVESLTSF LNLVR
Length:465
Mass (Da):51,714
Last modified:October 19, 2002 - v2
Checksum:i7638DE9D9E4209CE
GO

Sequence cautioni

The sequence AAC49573 differs from that shown. Reason: Frameshift at position 453.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti67K → N in AAM65557 (Ref. 7) Curated1
Sequence conflicti86E → D in AAC49573 (PubMed:8917600).Curated1
Sequence conflicti249 – 253LDGGV → WMVEF in AAC49573 (PubMed:8917600).Curated5
Sequence conflicti371N → F in AAC49561 (PubMed:8917599).Curated1
Sequence conflicti392Missing in AAC49573 (PubMed:8917600).Curated1
Sequence conflicti400D → A in AAC49561 (PubMed:8917599).Curated1
Sequence conflicti407I → D in AAC49561 (PubMed:8917599).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53864 mRNA Translation: AAC49561.1
U43412 mRNA Translation: AAC49573.1 Frameshift.
AF016282 Genomic DNA Translation: AAC26979.1
AF074021 Genomic DNA Translation: AAD29775.1
AL161501 Genomic DNA Translation: CAB80826.1
CP002687 Genomic DNA Translation: AEE82402.1
AF424582 mRNA Translation: AAL11576.1
BT002612 mRNA Translation: AAO11528.1
AY088011 mRNA Translation: AAM65557.1
AK220828 mRNA Translation: BAD94133.1
PIRiB85058
RefSeqiNP_192370.1, NM_116699.3
UniGeneiAt.47507
At.59149

Genome annotation databases

EnsemblPlantsiAT4G04610.1; AT4G04610.1; AT4G04610
GeneIDi825793
GrameneiAT4G04610.1; AT4G04610.1; AT4G04610
KEGGiath:AT4G04610

Similar proteinsi

Entry informationi

Entry nameiAPR1_ARATH
AccessioniPrimary (citable) accession number: P92979
Secondary accession number(s): O48886
, Q39248, Q56ZY2, Q8LA60
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: April 25, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health