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Reviewed, UniProtKB/Swiss-Prot P92976 (STS3_ARATH)

Last modified November 3, 2009. Version 67. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Strictosidine synthase 3
      Short name=SS-3
    EC=4.3.3.2
Gene names
Name: SS3
Ordered Locus Names: At1g74000
ORF Names: F2P9.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Catalyzes the stereospecific condensation of tryptamine with secologanin to form strictosidine, the key intermediate of indole alkaloid biosynthesis By similarity.

Catalytic activity

3-alpha-(S)-strictosidine + H2O = tryptamine + secologanin.

Pathway

Alkaloid biosynthesis; 3alpha(S)-strictosidine biosynthesis; 3alpha(S)-strictosidine from secologanin and tryptamine: step 1/1.

Subcellular location

Vacuole By similarity.

Sequence similarities

Belongs to the strictosidine synthase family.

Caution

It is uncertain whether Met-1 or Met-2 is the initiator.

Sequence caution

The sequence AAB40595.1 differs from that shown. Reason: Frameshift at positions 309 and 327.

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Ontologies

Keywords
   Biological processAlkaloid metabolism
   Cellular componentVacuole
   DomainSignal
   Molecular functionLyase
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processalkaloid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplant-type cell wall

Inferred from direct assay. Source: TAIR

vacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionstrictosidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 329306Strictosidine synthase 3
PRO_0000033331

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation791N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict54 – 552YT → LP in AAB40595. Ref.1
Sequence conflict1551T → N in AAB40595. Ref.1
Sequence conflict2011E → D in AAB40595. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P92976-1 [UniParc].

Last modified June 6, 2002. Version 2.
Checksum: C549547884B6E1C1

FASTA32934,667
        10         20         30         40         50         60 
MMRSFVSLIS LLLLLSFSSS VLSTKKSSFQ KLPVPGNRTG PEAFAFDSTG KGFYTGVTGG 

        70         80         90        100        110        120 
KILKYLPKKG YVDFAQITNS SKSSLCDGAL GTTNVEKCGR PAGIAFNTKT GDLYVADAAL 

       130        140        150        160        170        180 
GLHVIPRRGG LAKKIADSVG GKPFLFLDGL DVDPTTGVVY FTSFSSTFGP RDVLKAVATK 

       190        200        210        220        230        240 
DSTGKFFKYD PSKKVVTVLM EGLSGSAGCA VSSDGSFVLV GQFTKSNIKR YWIKGSKAGT 

       250        260        270        280        290        300 
SEDFTNSVSN PDNIKRIGST GNFWVASVVN SATGPTNPSA VKVSSAGKVL QTIPLKDKFG 

       310        320 
DTLVSEVNEY KGQLYIGALF GPFAGILKL

« Hide

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References

« Hide 'large scale' references
[1]Jain A.K., Nessler C.L.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed: 11130712] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

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Cross-references

Sequence databases

U43946 mRNA. Translation: AAB40595.1. Frameshift.
AC016662 Genomic DNA. Translation: AAG52519.1.
IPIIPI00537671.
PIRG96767.
RefSeqNP_177540.3.
UniGeneAt.18213

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP92976.

Proteomic databases

PRIDEP92976.

Genome annotation databases

GeneID843738.
GenomeReviewsGene locus AT1G74000 in contig CT485782_GR.
KEGGath:AT1G74000.

Organism-specific databases

TAIRAt1g74000.

Phylogenomic databases

OMAANTSEVF.

Enzyme and pathway databases

BRENDA4.3.3.2. 302.

Gene expression databases

ArrayExpressP92976.
GenevestigatorP92976.
GermOnlineAT1G74000. Arabidopsis thaliana.

Family and domain databases

InterProIPR011042. 6-blade_b-propeller_TolB-like.
IPR018119. Strictosidine_synth_cons-reg.
IPR004141. Strictosidine_synthase.
[Graphical view]
Gene3DG3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
PANTHERPTHR10426. Str_synth. 1 hit.
PfamPF03088. Str_synth. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameSTS3_ARATH
AccessionPrimary (citable) accession number: P92976
Secondary accession number(s): Q9C9C1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 6, 2002
Last modified: November 3, 2009
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents