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Protein

Ubiquitin-activating enzyme E1 2

Gene

UBA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.1 Publication

Miscellaneous

Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.1 Publication

Catalytic activityi

ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei499ATP; via amide nitrogenBy similarity1
Binding sitei525ATPBy similarity1
Binding sitei536ATPBy similarity1
Binding sitei549ATPBy similarity1
Active sitei653Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi597 – 598ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • cellular response to DNA damage stimulus Source: GO_Central
  • protein ubiquitination Source: TAIR

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B5 399
6.2.1.B9 399
ReactomeiR-ATH-8866652 Synthesis of active ubiquitin: roles of E1 and E2 enzymes
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-activating enzyme E1 2 (EC:6.2.1.451 Publication)
Short name:
AtUBA21 Publication
Gene namesi
Name:UBA2
Ordered Locus Names:At5g06460
ORF Names:MHF15.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G06460
TAIRilocus:2164270 AT5G06460

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

No visible phenotype and no changes in disease susceptibility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003993961 – 1077Ubiquitin-activating enzyme E1 2Add BLAST1077

Proteomic databases

PaxDbiP92974
PRIDEiP92974

PTM databases

iPTMnetiP92974

Expressioni

Tissue specificityi

Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication

Developmental stagei

Expressed over the entire range of development.1 Publication

Gene expression databases

ExpressionAtlasiP92974 baseline and differential
GenevisibleiP92974 AT

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G06460.1

Structurei

3D structure databases

ProteinModelPortaliP92974
SMRiP92974
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2012 Eukaryota
COG0476 LUCA
HOGENOMiHOG000167329
InParanoidiP92974
KOiK03178
OMAiSAPMCTL
OrthoDBiEOG0936017S
PhylomeDBiP92974

Family and domain databases

Gene3Di3.10.290.60, 1 hit
InterProiView protein in InterPro
IPR032420 E1_4HB
IPR032418 E1_FCCH
IPR000594 ThiF_NAD_FAD-bd
IPR018965 Ub-activating_enz_E1_C
IPR038252 UBA_E1_C_sf
IPR019572 UBA_E1_Cys
IPR035985 Ubiquitin-activating_enz
IPR018075 UBQ-activ_enz_E1
IPR018074 UBQ-activ_enz_E1_CS
IPR033127 UBQ-activ_enz_E1_Cys_AS
IPR000011 UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191 E1_4HB, 1 hit
PF16190 E1_FCCH, 1 hit
PF09358 E1_UFD, 1 hit
PF00899 ThiF, 2 hits
PF10585 UBA_e1_thiolCys, 1 hit
PRINTSiPR01849 UBIQUITINACT
SMARTiView protein in SMART
SM00985 UBA_e1_C, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01408 Ube1, 1 hit
PROSITEiView protein in PROSITE
PS00536 UBIQUITIN_ACTIVAT_1, 1 hit
PS00865 UBIQUITIN_ACTIVAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P92974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPFVVKENI IASASSPMKK RRIDHTESAD GSAINASNSS SIGLNNSIGG
60 70 80 90 100
NDTVMSMAEF GNDNSNNQEI DEDLHSRQLA VYGRETMRKL FASNVLISGM
110 120 130 140 150
QGLGVEIAKN IILAGVKSVT LHDENVVELW DLSSNFVFTE EDIGKNRALA
160 170 180 190 200
SVHKLQELNN AVAVSTLTGK LTKEQLSDFQ VVVFVDISFE KATEIDDYCH
210 220 230 240 250
SHQPPIAFIK ADVRGLFGSL FCDFGPHFTV LDVDGEEPHS GIIASVSNEN
260 270 280 290 300
PGFVSCVDDE RLEFEDGNLV VFSEVEGMTE LNDGKPRKIK NVKPFSFTLE
310 320 330 340 350
EDTSSYGQYM KGGIVTQVKQ PKVLNFKPLR EALKDPGDFL LSDFSKFDRP
360 370 380 390 400
PLLHLAFQAL DRFSSQAGRF PFAGSEEDAQ KLVEIAVDIN EGLGDARLED
410 420 430 440 450
VNSKLLRHLA FGSRAVLNPM AAMFGGIVGQ EVVKACSGKF HPIFQFFYFD
460 470 480 490 500
SVESLPKEPL DASEFRPQNS RYDAQISVFG STLQKKLEDA RVFVVGAGAL
510 520 530 540 550
GCEFLKNLAL MGVSCGTQGK LTVTDDDVIE KSNLSRQFLF RDWNIGQAKS
560 570 580 590 600
TVAATAAAGI NSRLNIDALQ NRVGPETENV FDDSFWENLT VVVNALDNVT
610 620 630 640 650
ARLYVDSRCV YFQKPLLESG TLGAKCNTQM VIPHLTENYG ASRDPPEKQA
660 670 680 690 700
PMCTVHSFPH NIDHCLTWAR SEFEGLLEKT PAEVNAYLSD PVEYMKAMRT
710 720 730 740 750
AGDAQARDTL GRVVECLEKE KCNSFQDCIT WARLRFEDYF ANRVKQLCYT
760 770 780 790 800
FPEDAATSTG APFWSAPKRF PRPLQFSSTD LSHINFVMAA SILRAETFGI
810 820 830 840 850
PTPEWAKTRA GLAEAVERVI VPDFEPKKDA TIVTDEKATT LSTASVDDAA
860 870 880 890 900
VIDELNAKLV RCRMSLQPEF RMKAIQFEKD DDTNYHMDMI AGLANMRARN
910 920 930 940 950
YSVPEVDKLK AKFIAGRIIP AIATSTAMAT GFVCLEMYKV LDGSHKVEDY
960 970 980 990 1000
RNTFANLALP LFSMAEPVPP KVVKHQDQSW TVWDRWVMRG NPTLRELLDW
1010 1020 1030 1040 1050
LKEKGLNAYS ISCGSSLLYN SMFSRHKERM NRRVVDLARD VAGVELPAYR
1060 1070
RHVDVVVACE DDNDADVDIP LVSVYFA
Length:1,077
Mass (Da):119,624
Last modified:May 1, 1997 - v1
Checksum:iCE39A36AAA99A218
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40566 Genomic DNA Translation: AAB37569.1
AB006700 Genomic DNA Translation: BAB08968.1
CP002688 Genomic DNA Translation: AED91020.1
RefSeqiNP_568168.1, NM_120729.4
UniGeneiAt.27859
At.65020

Genome annotation databases

EnsemblPlantsiAT5G06460.1; AT5G06460.1; AT5G06460
GeneIDi830534
GrameneiAT5G06460.1; AT5G06460.1; AT5G06460
KEGGiath:AT5G06460

Similar proteinsi

Entry informationi

Entry nameiUBE12_ARATH
AccessioniPrimary (citable) accession number: P92974
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health