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Protein

Ubiquitin-activating enzyme E1 2

Gene

UBA2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP.1 Publication

Catalytic activityi

ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei499 – 4991ATP; via amide nitrogenBy similarity
Binding sitei525 – 5251ATPBy similarity
Binding sitei536 – 5361ATPBy similarity
Binding sitei549 – 5491ATPBy similarity
Active sitei653 – 6531Glycyl thioester intermediatePROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi597 – 5982ATPBy similarity

GO - Molecular functioni

GO - Biological processi

  • protein ubiquitination Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.3.2.B5. 399.
6.2.1.B9. 399.
ReactomeiR-ATH-1169408. ISG15 antiviral mechanism.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-activating enzyme E1 2 (EC:6.2.1.451 Publication)
Short name:
AtUBA21 Publication
Gene namesi
Name:UBA2
Ordered Locus Names:At5g06460
ORF Names:MHF15.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G06460.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

No visible phenotype and no changes in disease susceptibility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10771077Ubiquitin-activating enzyme E1 2PRO_0000399396Add
BLAST

Proteomic databases

PaxDbiP92974.
PRIDEiP92974.

PTM databases

iPTMnetiP92974.

Expressioni

Tissue specificityi

Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls.1 Publication

Developmental stagei

Expressed over the entire range of development.1 Publication

Gene expression databases

GenevisibleiP92974. AT.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G06460.1.

Structurei

3D structure databases

ProteinModelPortaliP92974.
SMRiP92974. Positions 1-453, 483-956.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Phylogenomic databases

eggNOGiKOG2012. Eukaryota.
COG0476. LUCA.
HOGENOMiHOG000167329.
InParanoidiP92974.
KOiK03178.
OMAiFAMAEPI.
OrthoDBiEOG0936017S.
PhylomeDBiP92974.

Family and domain databases

Gene3Di1.10.10.520. 1 hit.
3.40.50.720. 5 hits.
InterProiIPR032420. E1_4HB.
IPR032418. E1_FCCH.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_E1_C.
IPR023318. Ub_act_enz_dom_a.
IPR019572. UBA_E1_Cys.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_CS.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF16191. E1_4HB. 1 hit.
PF16190. E1_FCCH. 1 hit.
PF09358. E1_UFD. 1 hit.
PF00899. ThiF. 2 hits.
PF10585. UBA_e1_thiolCys. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92974-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPFVVKENI IASASSPMKK RRIDHTESAD GSAINASNSS SIGLNNSIGG
60 70 80 90 100
NDTVMSMAEF GNDNSNNQEI DEDLHSRQLA VYGRETMRKL FASNVLISGM
110 120 130 140 150
QGLGVEIAKN IILAGVKSVT LHDENVVELW DLSSNFVFTE EDIGKNRALA
160 170 180 190 200
SVHKLQELNN AVAVSTLTGK LTKEQLSDFQ VVVFVDISFE KATEIDDYCH
210 220 230 240 250
SHQPPIAFIK ADVRGLFGSL FCDFGPHFTV LDVDGEEPHS GIIASVSNEN
260 270 280 290 300
PGFVSCVDDE RLEFEDGNLV VFSEVEGMTE LNDGKPRKIK NVKPFSFTLE
310 320 330 340 350
EDTSSYGQYM KGGIVTQVKQ PKVLNFKPLR EALKDPGDFL LSDFSKFDRP
360 370 380 390 400
PLLHLAFQAL DRFSSQAGRF PFAGSEEDAQ KLVEIAVDIN EGLGDARLED
410 420 430 440 450
VNSKLLRHLA FGSRAVLNPM AAMFGGIVGQ EVVKACSGKF HPIFQFFYFD
460 470 480 490 500
SVESLPKEPL DASEFRPQNS RYDAQISVFG STLQKKLEDA RVFVVGAGAL
510 520 530 540 550
GCEFLKNLAL MGVSCGTQGK LTVTDDDVIE KSNLSRQFLF RDWNIGQAKS
560 570 580 590 600
TVAATAAAGI NSRLNIDALQ NRVGPETENV FDDSFWENLT VVVNALDNVT
610 620 630 640 650
ARLYVDSRCV YFQKPLLESG TLGAKCNTQM VIPHLTENYG ASRDPPEKQA
660 670 680 690 700
PMCTVHSFPH NIDHCLTWAR SEFEGLLEKT PAEVNAYLSD PVEYMKAMRT
710 720 730 740 750
AGDAQARDTL GRVVECLEKE KCNSFQDCIT WARLRFEDYF ANRVKQLCYT
760 770 780 790 800
FPEDAATSTG APFWSAPKRF PRPLQFSSTD LSHINFVMAA SILRAETFGI
810 820 830 840 850
PTPEWAKTRA GLAEAVERVI VPDFEPKKDA TIVTDEKATT LSTASVDDAA
860 870 880 890 900
VIDELNAKLV RCRMSLQPEF RMKAIQFEKD DDTNYHMDMI AGLANMRARN
910 920 930 940 950
YSVPEVDKLK AKFIAGRIIP AIATSTAMAT GFVCLEMYKV LDGSHKVEDY
960 970 980 990 1000
RNTFANLALP LFSMAEPVPP KVVKHQDQSW TVWDRWVMRG NPTLRELLDW
1010 1020 1030 1040 1050
LKEKGLNAYS ISCGSSLLYN SMFSRHKERM NRRVVDLARD VAGVELPAYR
1060 1070
RHVDVVVACE DDNDADVDIP LVSVYFA
Length:1,077
Mass (Da):119,624
Last modified:May 1, 1997 - v1
Checksum:iCE39A36AAA99A218
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40566 Genomic DNA. Translation: AAB37569.1.
AB006700 Genomic DNA. Translation: BAB08968.1.
CP002688 Genomic DNA. Translation: AED91020.1.
RefSeqiNP_568168.1. NM_120729.3.
UniGeneiAt.27859.
At.65020.

Genome annotation databases

EnsemblPlantsiAT5G06460.1; AT5G06460.1; AT5G06460.
GeneIDi830534.
GrameneiAT5G06460.1; AT5G06460.1; AT5G06460.
KEGGiath:AT5G06460.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U40566 Genomic DNA. Translation: AAB37569.1.
AB006700 Genomic DNA. Translation: BAB08968.1.
CP002688 Genomic DNA. Translation: AED91020.1.
RefSeqiNP_568168.1. NM_120729.3.
UniGeneiAt.27859.
At.65020.

3D structure databases

ProteinModelPortaliP92974.
SMRiP92974. Positions 1-453, 483-956.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G06460.1.

PTM databases

iPTMnetiP92974.

Proteomic databases

PaxDbiP92974.
PRIDEiP92974.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G06460.1; AT5G06460.1; AT5G06460.
GeneIDi830534.
GrameneiAT5G06460.1; AT5G06460.1; AT5G06460.
KEGGiath:AT5G06460.

Organism-specific databases

TAIRiAT5G06460.

Phylogenomic databases

eggNOGiKOG2012. Eukaryota.
COG0476. LUCA.
HOGENOMiHOG000167329.
InParanoidiP92974.
KOiK03178.
OMAiFAMAEPI.
OrthoDBiEOG0936017S.
PhylomeDBiP92974.

Enzyme and pathway databases

UniPathwayiUPA00143.
BRENDAi2.3.2.B5. 399.
6.2.1.B9. 399.
ReactomeiR-ATH-1169408. ISG15 antiviral mechanism.

Miscellaneous databases

PROiP92974.

Gene expression databases

GenevisibleiP92974. AT.

Family and domain databases

Gene3Di1.10.10.520. 1 hit.
3.40.50.720. 5 hits.
InterProiIPR032420. E1_4HB.
IPR032418. E1_FCCH.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_E1_C.
IPR023318. Ub_act_enz_dom_a.
IPR019572. UBA_E1_Cys.
IPR018075. UBQ-activ_enz_E1.
IPR018074. UBQ-activ_enz_E1_CS.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF16191. E1_4HB. 1 hit.
PF16190. E1_FCCH. 1 hit.
PF09358. E1_UFD. 1 hit.
PF00899. ThiF. 2 hits.
PF10585. UBA_e1_thiolCys. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00536. UBIQUITIN_ACTIVAT_1. 1 hit.
PS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBE12_ARATH
AccessioniPrimary (citable) accession number: P92974
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.