P92974 (UBE12_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-activating enzyme E1 2
Short name=AtUBA2

Gene names

Name:	UBA2
Ordered Locus Names:	At5g06460
ORF Names:	MHF15.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1077 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Ref.1
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Monomer By similarity.
Tissue specificity	Expressed in leaves, flowers, roots and stems. Detected in germinating seeds, cotyledons, hypocotyls, vascular tissues, anthers, filaments, pollen, style, stigma, sepals, petals, ovary, developing ovules, funiculi and silique walls. Ref.1
Developmental stage	Expressed over the entire range of development. Ref.1
Disruption phenotype	No visible phenotype and no changes in disease susceptibility. Ref.4
Miscellaneous	Both UBA1 and UBA2 are able to activate ubiquitin and transfer it to the E2s with equal efficiency.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytosol Inferred from direct assay PubMed 21166475. Source: TAIR nucleus Traceable author statement Ref.1. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW small protein activating enzyme activity Inferred from electronic annotation. Source: InterPro ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1077	1077	Ubiquitin-activating enzyme E1 2		PRO_0000399396
Regions
	Nucleotide binding	499 – 528	30	ATP By similarity
Sites
	Active site	653	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P92974 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: CE39A36AAA99A218	FASTA	1,077	119,624
	10 20 30 40 50 60 MEPFVVKENI IASASSPMKK RRIDHTESAD GSAINASNSS SIGLNNSIGG NDTVMSMAEF 70 80 90 100 110 120 GNDNSNNQEI DEDLHSRQLA VYGRETMRKL FASNVLISGM QGLGVEIAKN IILAGVKSVT 130 140 150 160 170 180 LHDENVVELW DLSSNFVFTE EDIGKNRALA SVHKLQELNN AVAVSTLTGK LTKEQLSDFQ 190 200 210 220 230 240 VVVFVDISFE KATEIDDYCH SHQPPIAFIK ADVRGLFGSL FCDFGPHFTV LDVDGEEPHS 250 260 270 280 290 300 GIIASVSNEN PGFVSCVDDE RLEFEDGNLV VFSEVEGMTE LNDGKPRKIK NVKPFSFTLE 310 320 330 340 350 360 EDTSSYGQYM KGGIVTQVKQ PKVLNFKPLR EALKDPGDFL LSDFSKFDRP PLLHLAFQAL 370 380 390 400 410 420 DRFSSQAGRF PFAGSEEDAQ KLVEIAVDIN EGLGDARLED VNSKLLRHLA FGSRAVLNPM 430 440 450 460 470 480 AAMFGGIVGQ EVVKACSGKF HPIFQFFYFD SVESLPKEPL DASEFRPQNS RYDAQISVFG 490 500 510 520 530 540 STLQKKLEDA RVFVVGAGAL GCEFLKNLAL MGVSCGTQGK LTVTDDDVIE KSNLSRQFLF 550 560 570 580 590 600 RDWNIGQAKS TVAATAAAGI NSRLNIDALQ NRVGPETENV FDDSFWENLT VVVNALDNVT 610 620 630 640 650 660 ARLYVDSRCV YFQKPLLESG TLGAKCNTQM VIPHLTENYG ASRDPPEKQA PMCTVHSFPH 670 680 690 700 710 720 NIDHCLTWAR SEFEGLLEKT PAEVNAYLSD PVEYMKAMRT AGDAQARDTL GRVVECLEKE 730 740 750 760 770 780 KCNSFQDCIT WARLRFEDYF ANRVKQLCYT FPEDAATSTG APFWSAPKRF PRPLQFSSTD 790 800 810 820 830 840 LSHINFVMAA SILRAETFGI PTPEWAKTRA GLAEAVERVI VPDFEPKKDA TIVTDEKATT 850 860 870 880 890 900 LSTASVDDAA VIDELNAKLV RCRMSLQPEF RMKAIQFEKD DDTNYHMDMI AGLANMRARN 910 920 930 940 950 960 YSVPEVDKLK AKFIAGRIIP AIATSTAMAT GFVCLEMYKV LDGSHKVEDY RNTFANLALP 970 980 990 1000 1010 1020 LFSMAEPVPP KVVKHQDQSW TVWDRWVMRG NPTLRELLDW LKEKGLNAYS ISCGSSLLYN 1030 1040 1050 1060 1070 SMFSRHKERM NRRVVDLARD VAGVELPAYR RHVDVVVACE DDNDADVDIP LVSVYFA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ubiquitin-activating enzyme (E1) gene family in Arabidopsis thaliana." Hatfield P.M., Gosink M.M., Carpenter T.B., Vierstra R.D. Plant J. 11:213-226(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence features of the regions of 1,044,062 bp covered by thirteen physically assigned P1 clones." Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:291-300(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"The ubiquitin pathway is required for innate immunity in Arabidopsis." Goritschnig S., Zhang Y., Li X. Plant J. 49:540-551(2007) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U40566 Genomic DNA. Translation: AAB37569.1. AB006700 Genomic DNA. Translation: BAB08968.1. CP002688 Genomic DNA. Translation: AED91020.1.
IPI	IPI00540789.
RefSeq	NP_568168.1. NM_120729.3.
UniGene	At.27859. At.65020.
3D structure databases
HSSP	HSSP built from PDB template 1JW9 based on UniProtKB P12282.
ProteinModelPortal	P92974.
SMR	P92974. Positions 1-453, 483-956.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G06460.1-P.
Proteomic databases
PaxDb	P92974.
PRIDE	P92974.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G06460.1; AT5G06460.1; AT5G06460.
GeneID	830534.
KEGG	ath:AT5G06460.
Organism-specific databases
TAIR	At5g06460.
Phylogenomic databases
eggNOG	COG0476.
HOGENOM	HOG000167329.
InParanoid	P92974.
KO	K03178.
OMA	FESGDYV.
PhylomeDB	P92974.
ProtClustDB	CLSN2688565.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
ArrayExpress	P92974.
Genevestigator	P92974.
Family and domain databases
Gene3D	1.10.10.520. 1 hit. 3.40.50.720. 5 hits.
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. IPR018965. Ub-activating_enz_e1_C. IPR023318. Ub_act_enz_dom_a. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR018074. UBQ-activ_enz_E1_AS. IPR000011. UBQ/SUMO-activ_enz_E1-like. [Graphical view]
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
SMART	SM00985. UBA_e1_C. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 2 hits.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. 1 hit. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE12_ARATH

Accession

Primary (citable) accession number: P92974

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program