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Protein

Protein CCA1

Gene

CCA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor involved in the circadian clock and in the phytochrome regulation. Binds to the promoter regions of APRR1/TOC1 and TCP21/CHE to repress their transcription. Binds to the promoter regions of CAB2A and CAB2B to promote their transcription. Represses both LHY and itself.7 Publications

Miscellaneous

CCA1 and LHY are only partially redundant, but they bind to the same region of the promoters.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi46 – 69H-T-H motifPROSITE-ProRule annotationAdd BLAST24

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • circadian rhythm Source: TAIR
  • long-day photoperiodism, flowering Source: TAIR
  • negative regulation of circadian rhythm Source: TAIR
  • negative regulation of transcription, DNA-templated Source: TAIR
  • positive regulation of transcription, DNA-templated Source: TAIR
  • regulation of gene expression Source: TAIR
  • regulation of protein homodimerization activity Source: TAIR
  • response to abscisic acid Source: TAIR
  • response to auxin Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
  • response to ethylene Source: TAIR
  • response to gibberellin Source: TAIR
  • response to jasmonic acid Source: TAIR
  • response to organonitrogen compound Source: TAIR
  • response to salicylic acid Source: TAIR
  • response to salt stress Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDNA-binding
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CCA11 Publication
Alternative name(s):
MYB-related transcription factor CCA11 Publication
Protein CIRCADIAN CLOCK ASSOCIATED 11 Publication
Gene namesi
Name:CCA11 Publication
Ordered Locus Names:At2g46830Imported
ORF Names:F19D11Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G46830
TAIRilocus:2044345 AT2G46830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Altered phytochrome regulation and shorter circadian oscillations.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi5S → A: No effect on CKB3 binding, but loss of DNA binding; when associated with A-6; A-431; A-432; A-433 and A-484. 1 Publication1
Mutagenesisi6S → A: No effect on CKB3 binding, but loss of DNA binding; when associated with A-5; A-431; A-432; A-433 and A-484. 1 Publication1
Mutagenesisi431S → A: No effect on CKB3 binding; but loss of DNA binding; when associated with A-5; A-6; A-432; A-433 and A-484. 1 Publication1
Mutagenesisi432S → A: No effect on CKB3 binding, but loss of DNA binding; when associated with A-5; A-6; A-431; A-433 and A-484. 1 Publication1
Mutagenesisi433S → A: No effect on CKB3 binding, but loss of DNA binding; when associated with A-5; A-6; A-431; A-432 and A-484. 1 Publication1
Mutagenesisi484S → A: No effect on CKB3 binding, but loss of DNA binding; when associated with A-5; A-6; A-431; A-432 and A-433. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003889971 – 608Protein CCA1Add BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei5Phosphoserine; by CK21 Publication1
Modified residuei6Phosphoserine; by CK21 Publication1

Post-translational modificationi

Phosphorylated at Ser-5, Ser-6, and at least at one of the position Ser-431, Ser-432, Ser-433 or Ser-484. Phosphorylation is involved in dimerization and is necessary for binding to promoters and regulation of the circadian clock.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP92973
PRIDEiP92973

PTM databases

iPTMnetiP92973

Expressioni

Tissue specificityi

Expressed in leaves, roots, stems, flowers and siliques.2 Publications

Inductioni

Circadian-regulation with peak levels occurring around 1 hour after dawn. Up-regulated by APRR1/TOC1 and transiently by light treatment. Down-regulated by APRR5, APRR7 and APRR9. The CCA1 mRNA is relatively stable in the dark and in far-red light but has a short half-life in red and blue light.7 Publications

Gene expression databases

ExpressionAtlasiP92973 baseline and differential
GenevisibleiP92973 AT

Interactioni

Subunit structurei

Homodimer or heterodimer with LHY. Interacts with LHY; independently of photoperiod. Probably part of a large complex. Interacts with CKB1, CKB2 and CKB3. Interacts with LNK1 and LNK2 (PubMed:25012192).5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4631, 18 interactors
IntActiP92973, 4 interactors
STRINGi3702.AT2G46830.1

Structurei

3D structure databases

ProteinModelPortaliP92973
SMRiP92973
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 73HTH myb-typePROSITE-ProRule annotationAdd BLAST55

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi308 – 324Ala-richAdd BLAST17
Compositional biasi517 – 522Poly-Gln6

Domaini

The N-terminal part (11-82) is essential for DNA binding, an internal domain (136-316) is important for homodimerization and for interaction with LHY and the C-terminal part (317-608) is necessary for interaction with CKB3.

Phylogenomic databases

eggNOGiKOG0724 Eukaryota
COG5269 LUCA
HOGENOMiHOG000084594
InParanoidiP92973
KOiK12134
OMAiHNRFIEA
OrthoDBiEOG0936096P
PhylomeDBiP92973

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR009057 Homeobox-like_sf
IPR017930 Myb_dom
IPR006447 Myb_dom_plants
IPR001005 SANT/Myb
PfamiView protein in Pfam
PF00249 Myb_DNA-binding, 1 hit
SMARTiView protein in SMART
SM00717 SANT, 1 hit
SUPFAMiSSF46689 SSF46689, 1 hit
TIGRFAMsiTIGR01557 myb_SHAQKYF, 1 hit
PROSITEiView protein in PROSITE
PS51294 HTH_MYB, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P92973-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METNSSGEDL VIKTRKPYTI TKQRERWTEE EHNRFIEALR LYGRAWQKIE
60 70 80 90 100
EHVATKTAVQ IRSHAQKFFS KVEKEAEAKG VAMGQALDIA IPPPRPKRKP
110 120 130 140 150
NNPYPRKTGS GTILMSKTGV NDGKESLGSE KVSHPEMANE DRQQSKPEEK
160 170 180 190 200
TLQEDNCSDC FTHQYLSAAS SMNKSCIETS NASTFREFLP SREEGSQNNR
210 220 230 240 250
VRKESNSDLN AKSLENGNEQ GPQTYPMHIP VLVPLGSSIT SSLSHPPSEP
260 270 280 290 300
DSHPHTVAGD YQSFPNHIMS TLLQTPALYT AATFASSFWP PDSSGGSPVP
310 320 330 340 350
GNSPPNLAAM AAATVAAASA WWAANGLLPL CAPLSSGGFT SHPPSTFGPS
360 370 380 390 400
CDVEYTKAST LQHGSVQSRE QEHSEASKAR SSLDSEDVEN KSKPVCHEQP
410 420 430 440 450
SATPESDAKG SDGAGDRKQV DRSSCGSNTP SSSDDVEADA SERQEDGTNG
460 470 480 490 500
EVKETNEDTN KPQTSESNAR RSRISSNITD PWKSVSDEGR IAFQALFSRE
510 520 530 540 550
VLPQSFTYRE EHREEEQQQQ EQRYPMALDL NFTAQLTPVD DQEEKRNTGF
560 570 580 590 600
LGIGLDASKL MSRGRTGFKP YKRCSMEAKE SRILNNNPII HVEQKDPKRM

RLETQAST
Length:608
Mass (Da):66,976
Last modified:May 1, 1997 - v1
Checksum:i083C963EE6DD53A6
GO
Isoform 2 (identifier: P92973-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Show »
Length:526
Mass (Da):57,347
Checksum:i9E9739AE1200ED0F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383911 – 82Missing in isoform 2. 1 PublicationAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U28422 mRNA Translation: AAB40525.1
U79156 Genomic DNA Translation: AAC98813.1
AY519511 mRNA Translation: AAS09981.1
AC005310 Genomic DNA Translation: AAC33507.1
AC005310 Genomic DNA Translation: AAM15022.1
CP002685 Genomic DNA Translation: AEC10760.1
CP002685 Genomic DNA Translation: AEC10761.1
CP002685 Genomic DNA Translation: ANM61754.1
BT001096 mRNA Translation: AAN61004.1
BT001105 mRNA Translation: AAN64169.1
PIRiT02684
RefSeqiNP_001318437.1, NM_001337221.1 [P92973-2]
NP_850460.1, NM_180129.4 [P92973-1]
NP_850461.1, NM_180130.2 [P92973-2]
UniGeneiAt.10956

Genome annotation databases

EnsemblPlantsiAT2G46830.1; AT2G46830.1; AT2G46830 [P92973-1]
AT2G46830.2; AT2G46830.2; AT2G46830 [P92973-2]
AT2G46830.3; AT2G46830.3; AT2G46830 [P92973-2]
GeneIDi819296
GrameneiAT2G46830.1; AT2G46830.1; AT2G46830 [P92973-1]
AT2G46830.2; AT2G46830.2; AT2G46830 [P92973-2]
AT2G46830.3; AT2G46830.3; AT2G46830 [P92973-2]
KEGGiath:AT2G46830

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCCA1_ARATH
AccessioniPrimary (citable) accession number: P92973
Secondary accession number(s): Q8S8N5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 1, 1997
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names

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