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Protein

Potassium channel KAT3

Gene

KAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable modulatory (alpha) subunit of inward-rectifying potassium channels. Could mediate potassium uptake from the soil solution by plant roots in association with AKT1.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi406 – 527122cNMPAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • high-affinity potassium ion import Source: TAIR
  • regulation of membrane potential Source: GO_Central
  • response to nematode Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Potassium channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Protein family/group databases

TCDBi1.A.1.4.9. the voltage-gated ion channel (vic) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel KAT3
Alternative name(s):
AKT4
AtKC1
Potassium channel TKC
Gene namesi
Name:KAT3
Synonyms:AKT4, KC1
Ordered Locus Names:At4g32650
ORF Names:F4D11.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G32650.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9090CytoplasmicSequence analysisAdd
BLAST
Transmembranei91 – 11121Helical; Name=Segment S1Sequence analysisAdd
BLAST
Topological domaini112 – 1187ExtracellularSequence analysis
Transmembranei119 – 13921Helical; Name=Segment S2Sequence analysisAdd
BLAST
Topological domaini140 – 16324CytoplasmicSequence analysisAdd
BLAST
Transmembranei164 – 18421Helical; Name=Segment S3Sequence analysisAdd
BLAST
Topological domaini185 – 19410ExtracellularSequence analysis
Transmembranei195 – 21521Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd
BLAST
Topological domaini216 – 22914CytoplasmicSequence analysisAdd
BLAST
Transmembranei230 – 25021Helical; Name=Segment S5Sequence analysisAdd
BLAST
Topological domaini251 – 27727ExtracellularSequence analysisAdd
BLAST
Intramembranei278 – 29720Pore-forming; Name=Segment H5Sequence analysisAdd
BLAST
Topological domaini298 – 3014ExtracellularSequence analysis
Transmembranei302 – 32221Helical; Name=Segment S6Sequence analysisAdd
BLAST
Topological domaini323 – 662340CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 662662Potassium channel KAT3PRO_0000054127Add
BLAST

Proteomic databases

PaxDbiP92960.
PRIDEiP92960.

Expressioni

Tissue specificityi

Expressed predominantly in root hairs and root endodermis and, at a lower level, in leaf nodes, trichomes, and hydathodes.2 Publications

Inductioni

Strongly reduced in roots after 2,4-dichlorophenoxyacetic acid (2,4-D) treatment and after benzyladenine (BA) treatment. Strongly induced in shoots after NaCl treatment.1 Publication

Gene expression databases

ExpressionAtlasiP92960. baseline and differential.
GenevisibleiP92960. AT.

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. May interact with AKT1 and AKT2.

Protein-protein interaction databases

BioGridi14686. 14 interactions.
IntActiP92960. 5 interactions.
STRINGi3702.AT4G32650.1.

Structurei

3D structure databases

ProteinModelPortaliP92960.
SMRiP92960. Positions 347-509.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini591 – 66272KHAPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili528 – 55831Sequence analysisAdd
BLAST

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
Contains 1 KHA domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238230.
InParanoidiP92960.
OMAiRISHSHM.
OrthoDBiEOG093605FF.
PhylomeDBiP92960.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P92960-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTTTTEARS PLPLLLRRGR SSTALSASTA EARSPLSILQ FRRRSSKDVR
60 70 80 90 100
NITSVSSSLL PAFGTFIEDD NPSSKPFIVL HFDRRYRLWE LFLVILVGYS
110 120 130 140 150
AWASLFELAF EKAAEGALLT IDLVVDFFFA VDIILTFFVS YLDNTTYLNV
160 170 180 190 200
TDHKLIAKRY LKSVAFVMDV ASTLPIQFIY KTITGDVGRG QAFGFLNLLR
210 220 230 240 250
LWRLRRVAEL FKRLEKDAHF NYFVIRVIKL LCVTIFWIHL AGCILYWIAY
260 270 280 290 300
HYPRPTDTWI GSQVEDFKER SVWLGYTYSM YWSIVTLTTV GYGDLHAVNS
310 320 330 340 350
REKTFNMFYM LFNIGLTSYI IGIMTNLVVH GALRTFAMRS AINDILRYTS
360 370 380 390 400
KNRLPDTMRE QMLAHMQLKF KTAELRQEEV LQDLPKAIRS SINQHLFRSI
410 420 430 440 450
IEEAYLFKGF PEGLLVQLVS QIQAEYFPPK MEIILQNEIP TDFYVIVSGG
460 470 480 490 500
VDIIASKGVS EQVLAKLGPG SMAGEIGVVF NIPQPFTVRT RRLSQVIRIG
510 520 530 540 550
HHKFKEMVQS DNDVDAKMII ANFMTYLKGL NDELKKEIPF LRDLLDDADA
560 570 580 590 600
QVQETVQSEE TPQSNDEEIV TVSRHENGQI EERRREGVPK RVIIHGQAPP
610 620 630 640 650
NQDNKNNGDS NGRLIILPDS IQLLFDLAEK KLGKRGSTIA MADGAHVEQI
660
DALRENDHLY IF
Length:662
Mass (Da):75,598
Last modified:May 1, 1997 - v1
Checksum:iA6E90C9EB48387AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83202 mRNA. Translation: CAB05669.1.
U73325 Genomic DNA. Translation: AAC98810.1.
U81239 mRNA. Translation: AAD00503.1.
AL022537 Genomic DNA. Translation: CAA18596.1.
AL161581 Genomic DNA. Translation: CAB79982.1.
CP002687 Genomic DNA. Translation: AEE86097.1.
AY052233 mRNA. Translation: AAK97703.1.
BT003093 mRNA. Translation: AAO23890.1.
PIRiT04461.
RefSeqiNP_194991.1. NM_119417.3. [P92960-1]
UniGeneiAt.100.

Genome annotation databases

EnsemblPlantsiAT4G32650.1; AT4G32650.1; AT4G32650. [P92960-1]
GeneIDi829400.
KEGGiath:AT4G32650.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83202 mRNA. Translation: CAB05669.1.
U73325 Genomic DNA. Translation: AAC98810.1.
U81239 mRNA. Translation: AAD00503.1.
AL022537 Genomic DNA. Translation: CAA18596.1.
AL161581 Genomic DNA. Translation: CAB79982.1.
CP002687 Genomic DNA. Translation: AEE86097.1.
AY052233 mRNA. Translation: AAK97703.1.
BT003093 mRNA. Translation: AAO23890.1.
PIRiT04461.
RefSeqiNP_194991.1. NM_119417.3. [P92960-1]
UniGeneiAt.100.

3D structure databases

ProteinModelPortaliP92960.
SMRiP92960. Positions 347-509.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi14686. 14 interactions.
IntActiP92960. 5 interactions.
STRINGi3702.AT4G32650.1.

Protein family/group databases

TCDBi1.A.1.4.9. the voltage-gated ion channel (vic) superfamily.

Proteomic databases

PaxDbiP92960.
PRIDEiP92960.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G32650.1; AT4G32650.1; AT4G32650. [P92960-1]
GeneIDi829400.
KEGGiath:AT4G32650.

Organism-specific databases

TAIRiAT4G32650.

Phylogenomic databases

eggNOGiKOG0498. Eukaryota.
ENOG410XPSE. LUCA.
HOGENOMiHOG000238230.
InParanoidiP92960.
OMAiRISHSHM.
OrthoDBiEOG093605FF.
PhylomeDBiP92960.

Enzyme and pathway databases

ReactomeiR-ATH-1296072. Voltage gated Potassium channels.

Miscellaneous databases

PROiP92960.

Gene expression databases

ExpressionAtlasiP92960. baseline and differential.
GenevisibleiP92960. AT.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
InterProiIPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR003938. K_chnl_volt-dep_EAG/ELK/ERG.
IPR021789. KHA_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
PF11834. KHA. 1 hit.
[Graphical view]
PRINTSiPR01463. EAGCHANLFMLY.
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS51490. KHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKAT3_ARATH
AccessioniPrimary (citable) accession number: P92960
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.