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Protein

Potassium channel KAT3

Gene

KAT3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Probable modulatory (alpha) subunit of inward-rectifying potassium channels. Could mediate potassium uptake from the soil solution by plant roots in association with AKT1.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi406 – 527cNMPAdd BLAST122

GO - Molecular functioni

GO - Biological processi

  • potassium ion transmembrane transport Source: TAIR
  • regulation of ion transmembrane transport Source: UniProtKB-KW
  • regulation of membrane potential Source: GO_Central
  • response to nematode Source: TAIR

Keywordsi

Molecular functionIon channel, Potassium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Transport
LigandPotassium

Protein family/group databases

TCDBi1.A.1.4.9 the voltage-gated ion channel (vic) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium channel KAT3
Alternative name(s):
AKT4
AtKC1
Potassium channel TKC
Gene namesi
Name:KAT3
Synonyms:AKT4, KC1
Ordered Locus Names:At4g32650
ORF Names:F4D11.150
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G32650
TAIRilocus:2005524 AT4G32650

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 90CytoplasmicSequence analysisAdd BLAST90
Transmembranei91 – 111Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini112 – 118ExtracellularSequence analysis7
Transmembranei119 – 139Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini140 – 163CytoplasmicSequence analysisAdd BLAST24
Transmembranei164 – 184Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini185 – 194ExtracellularSequence analysis10
Transmembranei195 – 215Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini216 – 229CytoplasmicSequence analysisAdd BLAST14
Transmembranei230 – 250Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini251 – 277ExtracellularSequence analysisAdd BLAST27
Intramembranei278 – 297Pore-forming; Name=Segment H5Sequence analysisAdd BLAST20
Topological domaini298 – 301ExtracellularSequence analysis4
Transmembranei302 – 322Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini323 – 662CytoplasmicSequence analysisAdd BLAST340

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541271 – 662Potassium channel KAT3Add BLAST662

Proteomic databases

PaxDbiP92960
PRIDEiP92960

Expressioni

Tissue specificityi

Expressed predominantly in root hairs and root endodermis and, at a lower level, in leaf nodes, trichomes, and hydathodes.2 Publications

Inductioni

Strongly reduced in roots after 2,4-dichlorophenoxyacetic acid (2,4-D) treatment and after benzyladenine (BA) treatment. Strongly induced in shoots after NaCl treatment.1 Publication

Gene expression databases

ExpressionAtlasiP92960 baseline and differential
GenevisibleiP92960 AT

Interactioni

Subunit structurei

The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. May interact with AKT1 and AKT2 (PubMed:12678562). Interacts with SLAC1 (PubMed:27002025).2 Publications

Protein-protein interaction databases

BioGridi14686, 19 interactors
IntActiP92960, 5 interactors
STRINGi3702.AT4G32650.1

Structurei

3D structure databases

ProteinModelPortaliP92960
SMRiP92960
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini591 – 662KHAPROSITE-ProRule annotationAdd BLAST72

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili528 – 558Sequence analysisAdd BLAST31

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids. The pore-forming region H5 is enclosed by the transmembrane segments S5 and S6 in the Shaker-type (1P/6TM) and contains the GYGD signature motif which seems to be involved in potassium selectivity.
The KHA domain (rich in hydrophobic and acidic residues) present in the C-terminal part is likely to be important for tetramerization.

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOGENOMiHOG000238230
InParanoidiP92960
KOiK21867
OMAiMRHFKVK
OrthoDBiEOG093605FF
PhylomeDBiP92960

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR000595 cNMP-bd_dom
IPR005821 Ion_trans_dom
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR021789 KHA_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF11834 KHA, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS50042 CNMP_BINDING_3, 1 hit
PS51490 KHA, 1 hit

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P92960-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTTTTEARS PLPLLLRRGR SSTALSASTA EARSPLSILQ FRRRSSKDVR
60 70 80 90 100
NITSVSSSLL PAFGTFIEDD NPSSKPFIVL HFDRRYRLWE LFLVILVGYS
110 120 130 140 150
AWASLFELAF EKAAEGALLT IDLVVDFFFA VDIILTFFVS YLDNTTYLNV
160 170 180 190 200
TDHKLIAKRY LKSVAFVMDV ASTLPIQFIY KTITGDVGRG QAFGFLNLLR
210 220 230 240 250
LWRLRRVAEL FKRLEKDAHF NYFVIRVIKL LCVTIFWIHL AGCILYWIAY
260 270 280 290 300
HYPRPTDTWI GSQVEDFKER SVWLGYTYSM YWSIVTLTTV GYGDLHAVNS
310 320 330 340 350
REKTFNMFYM LFNIGLTSYI IGIMTNLVVH GALRTFAMRS AINDILRYTS
360 370 380 390 400
KNRLPDTMRE QMLAHMQLKF KTAELRQEEV LQDLPKAIRS SINQHLFRSI
410 420 430 440 450
IEEAYLFKGF PEGLLVQLVS QIQAEYFPPK MEIILQNEIP TDFYVIVSGG
460 470 480 490 500
VDIIASKGVS EQVLAKLGPG SMAGEIGVVF NIPQPFTVRT RRLSQVIRIG
510 520 530 540 550
HHKFKEMVQS DNDVDAKMII ANFMTYLKGL NDELKKEIPF LRDLLDDADA
560 570 580 590 600
QVQETVQSEE TPQSNDEEIV TVSRHENGQI EERRREGVPK RVIIHGQAPP
610 620 630 640 650
NQDNKNNGDS NGRLIILPDS IQLLFDLAEK KLGKRGSTIA MADGAHVEQI
660
DALRENDHLY IF
Length:662
Mass (Da):75,598
Last modified:May 1, 1997 - v1
Checksum:iA6E90C9EB48387AC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z83202 mRNA Translation: CAB05669.1
U73325 Genomic DNA Translation: AAC98810.1
U81239 mRNA Translation: AAD00503.1
AL022537 Genomic DNA Translation: CAA18596.1
AL161581 Genomic DNA Translation: CAB79982.1
CP002687 Genomic DNA Translation: AEE86097.1
AY052233 mRNA Translation: AAK97703.1
BT003093 mRNA Translation: AAO23890.1
PIRiT04461
RefSeqiNP_194991.1, NM_119417.4 [P92960-1]
UniGeneiAt.100

Genome annotation databases

EnsemblPlantsiAT4G32650.1; AT4G32650.1; AT4G32650 [P92960-1]
GeneIDi829400
GrameneiAT4G32650.1; AT4G32650.1; AT4G32650 [P92960-1]
KEGGiath:AT4G32650

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKAT3_ARATH
AccessioniPrimary (citable) accession number: P92960
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: May 1, 1997
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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