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Protein

SNF1-related protein kinase catalytic subunit alpha KIN11

Gene

KIN11

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants. The SnRK complex may also be involved in the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase and in assimilation of nitrogen by phosphorylating nitrate reductase. In vitro, KIN11 exhibits kinase activity on sucrose phosphate synthase and the kinase activity is inhibited by PRL1. May be a subunit of a SCF ubiquitin ligase complex and thus be involved in proteasomal ubiquitination. Involved in innate antiviral defenses.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Inactivated by the begomovirus AL2 protein or the curtovirus L2 protein. Activated by phosphorylation at Thr-176.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49ATPPROSITE-ProRule annotation1
Active sitei143Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi26 – 34ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antiviral protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Fatty acid biosynthesis, Fatty acid metabolism, Host-virus interaction, Lipid biosynthesis, Lipid metabolism, Nitrate assimilation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Names & Taxonomyi

Protein namesi
Recommended name:
SNF1-related protein kinase catalytic subunit alpha KIN11 (EC:2.7.11.1)
Short name:
AKIN11
Alternative name(s):
AKIN alpha-1
Short name:
AKINalpha1
Gene namesi
Name:KIN11
Synonyms:SNF1, SNRK1.2
Ordered Locus Names:At3g29160
ORF Names:MXE2.16
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G29160.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000861291 – 512SNF1-related protein kinase catalytic subunit alpha KIN11Add BLAST512

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei165PhosphoserineBy similarity1
Modified residuei176Phosphothreonine; by GRIK1 or GRIK21 Publication1

Post-translational modificationi

Autophosphorylated. Phosphorylated at Thr-176 by GRIK1/SNAK2 and GRIK2/SNAK1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP92958.

PTM databases

iPTMnetiP92958.

Expressioni

Tissue specificityi

Expressed in roots, shoots, flower buds, flowers, siliques and leaves.1 Publication

Inductioni

Induced by sucrose.1 Publication

Gene expression databases

GenevisibleiP92958. AT.

Interactioni

Subunit structurei

Subunit of a probable heterotrimeric complex consisting of an alpha catalytic (KIN10 or KIN11) subunit, and a beta (KINB) and a gamma (KING or SNF4) non-catalytic regulatory subunits. Interacts with KINB2, KINB3, SNF4 and probably with KINB1 and KING1. Interacts with SKP1/ASK1, PAD1 and the N-terminus of PRL1. Potential subunit of a SCF ubiquitin ligase complex consisting of a SNF1-related protein kinase, SKP1 and CUL1. The association of the SCF complex with the proteasome may be mediated by PAD1 and seems to be inhibited by the interaction with PRL1. Interacts with DSP4. Interacts with the begomovirus AL2 protein and the curtovirus L2 protein.8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
DSP4Q9FEB52EBI-307202,EBI-307215
KINB2Q9SCY54EBI-307202,EBI-2042436
PRL1Q423842EBI-307202,EBI-1382964
TOE2Q9LVG22EBI-307202,EBI-4424568

Protein-protein interaction databases

BioGridi7895. 43 interactors.
IntActiP92958. 30 interactors.
STRINGi3702.AT3G29160.1.

Structurei

3D structure databases

ProteinModelPortaliP92958.
SMRiP92958.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 272Protein kinasePROSITE-ProRule annotationAdd BLAST253
Domaini293 – 333UBAPROSITE-ProRule annotationAdd BLAST41
Domaini463 – 511KA1PROSITE-ProRule annotationAdd BLAST49

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni399 – 512Interaction with PAD1 and SKP11 PublicationAdd BLAST114

Sequence similaritiesi

Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 UBA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiP92958.
KOiK07198.
OMAiQLHFRDE.
OrthoDBiEOG0936066G.
PhylomeDBiP92958.

Family and domain databases

InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P92958-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHSSNRFGN NGVESILPNY KLGKTLGIGS FGKVKIAEHV VTGHKVAIKI
60 70 80 90 100
LNRRKIKNME MEEKVRREIK ILRLFMHPHI IRQYEVIETT SDIYVVMEYV
110 120 130 140 150
KSGELFDYIV EKGRLQEDEA RNFFQQIISG VEYCHRNMVV HRDLKPENLL
160 170 180 190 200
LDSRCNIKIA DFGLSNVMRD GHFLKTSCGS PNYAAPEVIS GKLYAGPEVD
210 220 230 240 250
VWSCGVILYA LLCGTLPFDD ENIPNLFKKI KGGIYTLPSH LSSEARDLIP
260 270 280 290 300
RMLIVDPVKR ITIPEIRQHR WFQTHLPRYL AVSPPDTVEQ AKKINEEIVQ
310 320 330 340 350
EVVNMGFDRN QVLESLRNRT QNDATVTYYL LLDNRFRVPS GYLESEFQET
360 370 380 390 400
TDSGSNPMRT PEAGASPVGH WIPAHVDHYG LGARSQVPVD RKWALGLQSH
410 420 430 440 450
AHPREIMNEV LKALQELNVC WKKIGHYNMK CRWVPGLADG QNTMVNNQLH
460 470 480 490 500
FRDESSIIED DCAMTSPTVI KFELQLYKAR EEKYLLDIQR VNGPQFLFLD
510
LCAAFLTELR VI
Length:512
Mass (Da):58,690
Last modified:May 1, 1997 - v1
Checksum:i4FBEF55D3EC60F87
GO
Isoform 2 (identifier: P92958-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     352-359: DSGSNPMR → WFQSYAHT
     360-512: Missing.

Note: Derived from EST data. No experimental confirmation available.
Show »
Length:359
Mass (Da):41,434
Checksum:i719F2FC270AEF250
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti291A → T in CAA64382 (PubMed:10417704).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_034567352 – 359DSGSNPMR → WFQSYAHT in isoform 2. Curated8
Alternative sequenceiVSP_034568360 – 512Missing in isoform 2. CuratedAdd BLAST153

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94755 mRNA. Translation: CAA64382.1.
X99279 mRNA. Translation: CAA67671.1.
DQ778956 mRNA. Translation: ABH11526.1.
AB018121 Genomic DNA. Translation: BAB01993.1.
CP002686 Genomic DNA. Translation: AEE77542.1.
CP002686 Genomic DNA. Translation: AEE77543.1.
CP002686 Genomic DNA. Translation: AEE77544.1.
AY070468 mRNA. Translation: AAL49934.1.
AY149927 mRNA. Translation: AAN31081.1.
PIRiT52633.
RefSeqiNP_566843.1. NM_113839.4. [P92958-1]
NP_974374.1. NM_202645.3. [P92958-1]
NP_974375.1. NM_202646.1. [P92958-2]
UniGeneiAt.184.

Genome annotation databases

EnsemblPlantsiAT3G29160.1; AT3G29160.1; AT3G29160. [P92958-1]
AT3G29160.2; AT3G29160.2; AT3G29160. [P92958-1]
GeneIDi822566.
GrameneiAT3G29160.1; AT3G29160.1; AT3G29160.
AT3G29160.2; AT3G29160.2; AT3G29160.
KEGGiath:AT3G29160.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X94755 mRNA. Translation: CAA64382.1.
X99279 mRNA. Translation: CAA67671.1.
DQ778956 mRNA. Translation: ABH11526.1.
AB018121 Genomic DNA. Translation: BAB01993.1.
CP002686 Genomic DNA. Translation: AEE77542.1.
CP002686 Genomic DNA. Translation: AEE77543.1.
CP002686 Genomic DNA. Translation: AEE77544.1.
AY070468 mRNA. Translation: AAL49934.1.
AY149927 mRNA. Translation: AAN31081.1.
PIRiT52633.
RefSeqiNP_566843.1. NM_113839.4. [P92958-1]
NP_974374.1. NM_202645.3. [P92958-1]
NP_974375.1. NM_202646.1. [P92958-2]
UniGeneiAt.184.

3D structure databases

ProteinModelPortaliP92958.
SMRiP92958.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7895. 43 interactors.
IntActiP92958. 30 interactors.
STRINGi3702.AT3G29160.1.

PTM databases

iPTMnetiP92958.

Proteomic databases

PaxDbiP92958.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G29160.1; AT3G29160.1; AT3G29160. [P92958-1]
AT3G29160.2; AT3G29160.2; AT3G29160. [P92958-1]
GeneIDi822566.
GrameneiAT3G29160.1; AT3G29160.1; AT3G29160.
AT3G29160.2; AT3G29160.2; AT3G29160.
KEGGiath:AT3G29160.

Organism-specific databases

TAIRiAT3G29160.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiP92958.
KOiK07198.
OMAiQLHFRDE.
OrthoDBiEOG0936066G.
PhylomeDBiP92958.

Enzyme and pathway databases

ReactomeiR-ATH-163680. AMPK inhibits chREBP transcriptional activation activity.
R-ATH-200425. Import of palmitoyl-CoA into the mitochondrial matrix.
R-ATH-380972. Energy dependent regulation of mTOR by LKB1-AMPK.

Miscellaneous databases

PROiP92958.

Gene expression databases

GenevisibleiP92958. AT.

Family and domain databases

InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
IPR015940. UBA.
IPR009060. UBA-like.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00627. UBA. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00165. UBA. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF46934. SSF46934. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50030. UBA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIN11_ARATH
AccessioniPrimary (citable) accession number: P92958
Secondary accession number(s): A6XGQ9, P92968, Q3E7R4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.