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Protein

Ferric reduction oxidase 2

Gene

FRO2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Flavocytochrome that transfers electrons across the plasma membrane to reduce ferric iron chelates to form soluble ferrous iron in the rhizosphere. May be involved in the delivery of iron to developing pollen grains. Acts also as a copper-chelate reductase. Involved in glycine betaine-mediated chilling tolerance and reactive oxygen species accumulation.4 Publications

Catalytic activityi

2 Fe(II)-siderophore + NAD+ + H+ = 2 Fe(III)-siderophore + NADH.

Cofactori

FADCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi219Iron (heme axial ligand)Curated1
Metal bindingi233Iron (heme axial ligand)Curated1
Metal bindingi293Iron (heme axial ligand)Curated1
Metal bindingi306Iron (heme axial ligand)Curated1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi381 – 384FADSequence analysis4
Nucleotide bindingi429 – 432NADSequence analysis4

GO - Molecular functioni

  • ferric-chelate reductase activity Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • ion transport Source: UniProtKB-KW
  • iron chelate transport Source: TAIR
  • iron ion homeostasis Source: TAIR
  • response to bacterium Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Ion transport, Transport

Keywords - Ligandi

FAD, Flavoprotein, Heme, Iron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciARA:AT1G01580-MONOMER.

Protein family/group databases

TCDBi5.B.1.4.4. the phagocyte (gp91(phox)) nadph oxidase family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferric reduction oxidase 2 (EC:1.16.1.7)
Short name:
AtFRO2
Alternative name(s):
Ferric-chelate reductase 2
Protein FERRIC CHELATE REDUCTASE DEFECTIVE 1
Gene namesi
Name:FRO2
Synonyms:FRD1
Ordered Locus Names:At1g01580
ORF Names:F22L4.12
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G01580.

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 28Cytoplasmic1 PublicationAdd BLAST28
Transmembranei29 – 48HelicalAdd BLAST20
Topological domaini49 – 74Extracellular1 PublicationAdd BLAST26
Transmembranei75 – 93HelicalAdd BLAST19
Topological domaini94 – 125Cytoplasmic1 PublicationAdd BLAST32
Transmembranei126 – 149HelicalAdd BLAST24
Topological domaini150 – 217Extracellular1 PublicationAdd BLAST68
Transmembranei218 – 241HelicalAdd BLAST24
Topological domaini242 – 291Cytoplasmic1 PublicationAdd BLAST50
Transmembranei292 – 316HelicalAdd BLAST25
Topological domaini317 – 338Extracellular1 PublicationAdd BLAST22
Transmembranei339 – 359HelicalAdd BLAST21
Topological domaini360 – 554Cytoplasmic1 PublicationAdd BLAST195
Transmembranei555 – 577HelicalAdd BLAST23
Topological domaini578 – 597Extracellular1 PublicationAdd BLAST20
Transmembranei598 – 619HelicalAdd BLAST22
Topological domaini620 – 725Cytoplasmic1 PublicationAdd BLAST106

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Impaired growth on media with no added iron. No response to glycine betaine in chilling assays.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi384T → M in frd1-3; loss of induced ferric-chelate reductase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004132001 – 725Ferric reduction oxidase 2Add BLAST725

Proteomic databases

PaxDbiP92949.
PRIDEiP92949.

PTM databases

iPTMnetiP92949.

Expressioni

Tissue specificityi

Expressed in the epidermal cells of the roots. High expression in lateral roots and root hairs. Detected in leaves, stems, siliques and in flowers in anthers and styles.5 Publications

Inductioni

Circadian-regulation. Up-regulated in roots by iron deficiency, carbon monoxide and by treatment with glycine betaine. Down-regulated by zinc, cadmium and cytokinin. Not regulated by copper.7 Publications

Interactioni

Protein-protein interaction databases

BioGridi24646. 1 interactor.
STRINGi3702.AT1G01580.1.

Structurei

3D structure databases

ProteinModelPortaliP92949.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini183 – 303Ferric oxidoreductaseAdd BLAST121
Domaini332 – 437FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi285 – 330Phe-richAdd BLAST46
Compositional biasi568 – 609Ile-richAdd BLAST42

Domaini

The C-terminus is probably located inside the membrane.1 Publication

Sequence similaritiesi

Belongs to the ferric reductase (FRE) family.Curated
Contains 1 FAD-binding FR-type domain.PROSITE-ProRule annotation
Contains 1 ferric oxidoreductase domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000006154.
InParanoidiP92949.
OMAiRMFELFY.
OrthoDBiEOG093603NP.
PhylomeDBiP92949.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92949-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEIEKSNNGG SNPSAGEEFK DMIKGVTKFL MMVIFLGTIM LWIMMPTLTY
60 70 80 90 100
RTKWLPHLRI KFGTSTYFGA TGTTLFMYMF PMMVVACLGC VYLHFKNRKS
110 120 130 140 150
PHHIDRETKG GVWSKLRKPM LVKGPLGIVS VTEITFLAMF VALLLWCFIT
160 170 180 190 200
YLRNSFATIT PKSAAAHDES LWQAKLESAA LRLGLIGNIC LAFLFLPVAR
210 220 230 240 250
GSSLLPAMGL TSESSIKYHI WLGHMVMALF TVHGLCYIIY WASMHEISQM
260 270 280 290 300
IMWDTKGVSN LAGEIALAAG LVMWATTYPK IRRRFFEVFF YTHYLYIVFM
310 320 330 340 350
LFFVLHVGIS FSFIALPGFY IFLVDRFLRF LQSRENVRLL AARILPSDTM
360 370 380 390 400
ELTFSKNSKL VYSPTSIMFV NIPSISKLQW HPFTITSSSK LEPEKLSIVI
410 420 430 440 450
KKEGKWSTKL HQRLSSSDQI DRLAVSVEGP YGPASADFLR HEALVMVCGG
460 470 480 490 500
SGITPFISVI RDLIATSQKE TCKIPKITLI CAFKKSSEIS MLDLVLPLSG
510 520 530 540 550
LETELSSDIN IKIEAFITRD NDAGDEAKAG KIKTLWFKPS LSDQSISSIL
560 570 580 590 600
GPNSWLWLGA ILASSFLIFM IIIGIITRYY IYPIDHNTNK IYSLTSKTII
610 620 630 640 650
YILVISVSIM ATCSAAMLWN KKKYGKVESK QVQNVDRPSP TSSPTSSWGY
660 670 680 690 700
NSLREIESTP QESLVQRTNL HFGERPNLKK LLLDVEGSSV GVLVCGPKKM
710 720
RQKVAEICSS GLAENLHFES ISFSW
Length:725
Mass (Da):81,501
Last modified:May 1, 1999 - v2
Checksum:i9589ABA88DD79512
GO

Sequence cautioni

The sequence AAF81316 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09581 Genomic DNA. Translation: CAA70770.1.
AY302057 mRNA. Translation: AAP51420.1.
AC061957 Genomic DNA. Translation: AAF81316.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27308.1.
PIRiE86146.
RefSeqiNP_171664.1. NM_100040.3.
UniGeneiAt.42640.

Genome annotation databases

EnsemblPlantsiAT1G01580.1; AT1G01580.1; AT1G01580.
GeneIDi839411.
GrameneiAT1G01580.1; AT1G01580.1; AT1G01580.
KEGGiath:AT1G01580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09581 Genomic DNA. Translation: CAA70770.1.
AY302057 mRNA. Translation: AAP51420.1.
AC061957 Genomic DNA. Translation: AAF81316.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27308.1.
PIRiE86146.
RefSeqiNP_171664.1. NM_100040.3.
UniGeneiAt.42640.

3D structure databases

ProteinModelPortaliP92949.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi24646. 1 interactor.
STRINGi3702.AT1G01580.1.

Protein family/group databases

TCDBi5.B.1.4.4. the phagocyte (gp91(phox)) nadph oxidase family.

PTM databases

iPTMnetiP92949.

Proteomic databases

PaxDbiP92949.
PRIDEiP92949.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G01580.1; AT1G01580.1; AT1G01580.
GeneIDi839411.
GrameneiAT1G01580.1; AT1G01580.1; AT1G01580.
KEGGiath:AT1G01580.

Organism-specific databases

TAIRiAT1G01580.

Phylogenomic databases

eggNOGiKOG0039. Eukaryota.
ENOG410XNZY. LUCA.
HOGENOMiHOG000006154.
InParanoidiP92949.
OMAiRMFELFY.
OrthoDBiEOG093603NP.
PhylomeDBiP92949.

Enzyme and pathway databases

BioCyciARA:AT1G01580-MONOMER.

Miscellaneous databases

PROiP92949.

Family and domain databases

InterProiIPR013112. FAD-bd_8.
IPR017927. Fd_Rdtase_FAD-bd.
IPR013130. Fe3_Rdtase_TM_dom.
IPR013121. Fe_red_NAD-bd_6.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF08022. FAD_binding_8. 1 hit.
PF01794. Ferric_reduct. 1 hit.
PF08030. NAD_binding_6. 1 hit.
[Graphical view]
SUPFAMiSSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRO2_ARATH
AccessioniPrimary (citable) accession number: P92949
Secondary accession number(s): Q9LMM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: May 1, 1999
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Post-transcriptionally regulated by iron. Coordinately regulated with the iron transport protein IRT1.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.