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Protein

Monodehydroascorbate reductase 5, mitochondrial

Gene

MDAR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate (MDA) to ascorbate, oxidizing NADH in the process. Can also use 2,4,6-trinitrotoluene (TNT) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates, but not 1-chloro-4-nitrobenzene (CNB).1 Publication

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.1 Publication

Cofactori

FADBy similarity

Enzyme regulationi

Redox regulation of the activity by thioredoxin TRXy1.1 Publication

Kineticsi

  1. KM=522 µM for 2,4,6-trinitrotoluene1 Publication
  2. KM=4.1 µM for monodehydroascorbate1 Publication
  3. KM=1254 µM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=0.143 mmol/min/mg enzyme with 2,4,6-trinitrotoluene as substrate1 Publication
  2. Vmax=190 mmol/min/mg enzyme with monodehydroascorbate as substrate1 Publication
  3. Vmax=0.960 mmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi62 – 79FADBy similarityAdd BLAST18
Nucleotide bindingi218 – 235NADBy similarityAdd BLAST18
Nucleotide bindingi244 – 248FADBy similarity5

GO - Molecular functioni

  • ATP binding Source: TAIR
  • monodehydroascorbate reductase (NADH) activity Source: UniProtKB-EC

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein, NAD

Enzyme and pathway databases

BioCyciARA:AT1G63940-MONOMER.
BRENDAi1.6.5.4. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase 5, mitochondrial1 Publication (EC:1.6.5.41 Publication)
Short name:
AtMDAR51 Publication
Alternative name(s):
Monodehydroascorbate reductase 6, chloroplastic1 Publication (EC:1.6.5.4)
Short name:
AtMDAR61 Publication
Gene namesi
Name:MDAR51 Publication
Synonyms:MDAR61 Publication, MDHAR61 Publication, PMDAR-L1 Publication, PMDAR-S1 Publication
Ordered Locus Names:At1g63940Imported
ORF Names:T12P18.4Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G63940.

Subcellular locationi

Isoform MDAR6 :
  • Plastidchloroplast 1 Publication
Isoform MDAR5 :
  • Mitochondrion 1 Publication

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast stroma Source: TAIR
  • mitochondrion Source: TAIR
  • stromule Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Biotechnological usei

The main mechanism for TNT toxicity in plants is the production of superoxide in the mitochondria by MDAR5 with TNT as a substrate. Inactivation of MDAR5 enhance TNT tolerance, thus enabling revegetation and remediation of explosives-contaminated sites.1 Publication

Disruption phenotypei

No visible phenotype, but enhanced 2,4,6-trinitrotoluene (TNT) tolerance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionSequence analysisAdd BLAST53
ChainiPRO_000001862154 – 493Monodehydroascorbate reductase 5, mitochondrialAdd BLAST440

Proteomic databases

PaxDbiP92947.
PRIDEiP92947.

PTM databases

iPTMnetiP92947.

Expressioni

Gene expression databases

ExpressionAtlasiP92947. baseline and differential.
GenevisibleiP92947. AT.

Interactioni

Subunit structurei

Interacts in vitro with TRXy.1 Publication

Protein-protein interaction databases

BioGridi27918. 2 interactors.
IntActiP92947. 2 interactors.
STRINGi3702.AT1G63940.2.

Structurei

3D structure databases

ProteinModelPortaliP92947.
SMRiP92947.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
HOGENOMiHOG000276711.
InParanoidiP92947.
KOiK08232.
OMAiVWWQFFG.
OrthoDBiEOG093609QX.
PhylomeDBiP92947.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform MDAR5 (identifier: P92947-1) [UniParc]FASTAAdd to basket
Also known as: PMDAR-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAVRRVMAL ASTTLPTKSG LSLWCPSSPS LARRFPARFS PIGSRIASRS
60 70 80 90 100
LVTASFANEN REFVIVGGGN AAGYAARTFV ENGMADGRLC IVTKEAYAPY
110 120 130 140 150
ERPALTKAYL FPPEKKPARL PGFHTCVGGG GERQTPDWYK EKGIEVIYED
160 170 180 190 200
PVAGADFEKQ TLTTDAGKQL KYGSLIIATG CTASRFPDKI GGHLPGVHYI
210 220 230 240 250
REVADADSLI ASLGKAKKIV IVGGGYIGME VAAAAVAWNL DTTIVFPEDQ
260 270 280 290 300
LLQRLFTPSL AQKYEELYRQ NGVKFVKGAS INNLEAGSDG RVSAVKLADG
310 320 330 340 350
STIEADTVVI GIGAKPAIGP FETLAMNKSI GGIQVDGLFR TSTPGIFAIG
360 370 380 390 400
DVAAFPLKIY DRMTRVEHVD HARRSAQHCV KSLLTAHTDT YDYLPYFYSR
410 420 430 440 450
VFEYEGSPRK VWWQFFGDNV GETVEVGNFD PKIATFWIES GRLKGVLVES
460 470 480 490
GSPEEFQLLP KLARSQPLVD KAKLASASSV EEALEIAQAA LQS
Length:493
Mass (Da):53,302
Last modified:July 19, 2004 - v3
Checksum:i44FBF0DE65DA89D7
GO
Isoform MDAR6 (identifier: P92947-2) [UniParc]FASTAAdd to basket
Also known as: PMDAR-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: Missing.

Show »
Length:486
Mass (Da):52,502
Checksum:i468FBFC7B05EAAAE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109Y → H in BAA12349 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0113601 – 7Missing in isoform MDAR6. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84417 mRNA. Translation: BAA12349.2.
AC010852 Genomic DNA. Translation: AAG52455.1.
CP002684 Genomic DNA. Translation: AEE34168.1.
CP002684 Genomic DNA. Translation: AEE34171.1.
AY034934 mRNA. Translation: AAK59441.1.
AY142572 mRNA. Translation: AAN13141.1.
BT000667 mRNA. Translation: AAN31814.1.
PIRiE96664.
RefSeqiNP_564818.1. NM_105067.4. [P92947-2]
NP_849839.1. NM_179508.3. [P92947-1]
UniGeneiAt.24374.
At.72711.

Genome annotation databases

EnsemblPlantsiAT1G63940.2; AT1G63940.2; AT1G63940. [P92947-1]
GeneIDi842697.
GrameneiAT1G63940.2; AT1G63940.2; AT1G63940.
KEGGiath:AT1G63940.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84417 mRNA. Translation: BAA12349.2.
AC010852 Genomic DNA. Translation: AAG52455.1.
CP002684 Genomic DNA. Translation: AEE34168.1.
CP002684 Genomic DNA. Translation: AEE34171.1.
AY034934 mRNA. Translation: AAK59441.1.
AY142572 mRNA. Translation: AAN13141.1.
BT000667 mRNA. Translation: AAN31814.1.
PIRiE96664.
RefSeqiNP_564818.1. NM_105067.4. [P92947-2]
NP_849839.1. NM_179508.3. [P92947-1]
UniGeneiAt.24374.
At.72711.

3D structure databases

ProteinModelPortaliP92947.
SMRiP92947.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi27918. 2 interactors.
IntActiP92947. 2 interactors.
STRINGi3702.AT1G63940.2.

PTM databases

iPTMnetiP92947.

Proteomic databases

PaxDbiP92947.
PRIDEiP92947.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G63940.2; AT1G63940.2; AT1G63940. [P92947-1]
GeneIDi842697.
GrameneiAT1G63940.2; AT1G63940.2; AT1G63940.
KEGGiath:AT1G63940.

Organism-specific databases

TAIRiAT1G63940.

Phylogenomic databases

eggNOGiKOG1336. Eukaryota.
COG0446. LUCA.
HOGENOMiHOG000276711.
InParanoidiP92947.
KOiK08232.
OMAiVWWQFFG.
OrthoDBiEOG093609QX.
PhylomeDBiP92947.

Enzyme and pathway databases

BioCyciARA:AT1G63940-MONOMER.
BRENDAi1.6.5.4. 399.

Miscellaneous databases

PROiP92947.

Gene expression databases

ExpressionAtlasiP92947. baseline and differential.
GenevisibleiP92947. AT.

Family and domain databases

Gene3Di3.50.50.60. 2 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
[Graphical view]
PfamiPF07992. Pyr_redox_2. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiMDAR5_ARATH
AccessioniPrimary (citable) accession number: P92947
Secondary accession number(s): Q94CE2, Q9CAK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: November 30, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The use of alternative transcription starts causes dual targeting of the same mature MDAR protein to both mitochondria and chloroplast.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.