Reviewed, UniProtKB/Swiss-Prot P92947 (MDARP_ARATH)

Names and origin

Protein names

Recommended name:
    Monodehydroascorbate reductase, chloroplastic
      Short name=MDAR
    EC=1.6.5.4

Gene names

Ordered Locus Names:	At1g63940
ORF Names:	T12P18.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process By similarity.
Catalytic activity	NADH + 2 monodehydroascorbate = NAD(+) + 2 ascorbate.
Cofactor	FAD By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Coding sequence diversity	Alternative splicing
Domain	Redox-active center Transit peptide
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro monodehydroascorbate reductase (NADH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P92947-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P92947-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Chloroplast Potential
	Chain	54 – 493	440	Monodehydroascorbate reductase, chloroplastic		PRO_0000018621
Natural variations
	Alternative sequence	1 – 7	7	Missing in isoform 2.		VSP_011360
Experimental info
	Sequence conflict	109	1	Y → H in BAA12349. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified July 19, 2004. Version 3. Checksum: 44FBF0DE65DA89D7	FASTA	493	53,302
	10 20 30 40 50 60 MSAVRRVMAL ASTTLPTKSG LSLWCPSSPS LARRFPARFS PIGSRIASRS LVTASFANEN 70 80 90 100 110 120 REFVIVGGGN AAGYAARTFV ENGMADGRLC IVTKEAYAPY ERPALTKAYL FPPEKKPARL 130 140 150 160 170 180 PGFHTCVGGG GERQTPDWYK EKGIEVIYED PVAGADFEKQ TLTTDAGKQL KYGSLIIATG 190 200 210 220 230 240 CTASRFPDKI GGHLPGVHYI REVADADSLI ASLGKAKKIV IVGGGYIGME VAAAAVAWNL 250 260 270 280 290 300 DTTIVFPEDQ LLQRLFTPSL AQKYEELYRQ NGVKFVKGAS INNLEAGSDG RVSAVKLADG 310 320 330 340 350 360 STIEADTVVI GIGAKPAIGP FETLAMNKSI GGIQVDGLFR TSTPGIFAIG DVAAFPLKIY 370 380 390 400 410 420 DRMTRVEHVD HARRSAQHCV KSLLTAHTDT YDYLPYFYSR VFEYEGSPRK VWWQFFGDNV 430 440 450 460 470 480 GETVEVGNFD PKIATFWIES GRLKGVLVES GSPEEFQLLP KLARSQPLVD KAKLASASSV 490 EEALEIAQAA LQS
	Isoform 2 [UniParc]. Checksum: 468FBFC7B05EAAAE		486	52,502
	10 20 30 40 50 60 MALASTTLPT KSGLSLWCPS SPSLARRFPA RFSPIGSRIA SRSLVTASFA NENREFVIVG 70 80 90 100 110 120 GGNAAGYAAR TFVENGMADG RLCIVTKEAY APYERPALTK AYLFPPEKKP ARLPGFHTCV 130 140 150 160 170 180 GGGGERQTPD WYKEKGIEVI YEDPVAGADF EKQTLTTDAG KQLKYGSLII ATGCTASRFP 190 200 210 220 230 240 DKIGGHLPGV HYIREVADAD SLIASLGKAK KIVIVGGGYI GMEVAAAAVA WNLDTTIVFP 250 260 270 280 290 300 EDQLLQRLFT PSLAQKYEEL YRQNGVKFVK GASINNLEAG SDGRVSAVKL ADGSTIEADT 310 320 330 340 350 360 VVIGIGAKPA IGPFETLAMN KSIGGIQVDG LFRTSTPGIF AIGDVAAFPL KIYDRMTRVE 370 380 390 400 410 420 HVDHARRSAQ HCVKSLLTAH TDTYDYLPYF YSRVFEYEGS PRKVWWQFFG DNVGETVEVG 430 440 450 460 470 480 NFDPKIATFW IESGRLKGVL VESGSPEEFQ LLPKLARSQP LVDKAKLASA SSVEEALEIA QAALQS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA of chloroplastic monodehydroascorbate radical reductase from Arabidopsis thaliana." Hossain A., Miyake C., Aoki H., Matsuo M., Yamaguchi T., Nishimura M., Ida S., Asada K. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84417 mRNA. Translation: BAA12349.2. AC010852 Genomic DNA. Translation: AAG52455.1. AY034934 mRNA. Translation: AAK59441.1. AY142572 mRNA. Translation: AAN13141.1. BT000667 mRNA. Translation: AAN31814.1.
PIR	E96664.
RefSeq	NP_849839.1.
UniGene	At.24374
3D structure databases
HSSP	HSSP built from PDB template 1M6I based on UniProtKB O95831.
ModBase	Search...
Genome annotation databases
GeneID	842697.
GenomeReviews	Gene locus AT1G63940 in contig CT485782_GR.
Organism-specific databases
TAIR	At1g63940.
Gene expression databases
ArrayExpress	P92947.
Family and domain databases
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR001100. Pyr_nuc-diS_OxRdtase. IPR001327. Pyr_OxRdtase_NAD_bd. [Graphical view]
Pfam	PF00070. Pyr_redox. 1 hit. PF07992. Pyr_redox_2. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR. PR00411. PNDRDTASEI.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

MDARP_ARATH

Accession

Primary (citable) accession number: P92947
Secondary accession number(s): Q94CE2, Q9CAK5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	July 19, 2004
Last modified:	November 25, 2008
This is version 60 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)