Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Monodehydroascorbate reductase, chloroplastic/mitochondrial

Gene

MDAR5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of monodehydroascorbate (MDA) to ascorbate, oxidizing NADH in the process. Can also use 2,4,6-trinitrotoluene (TNT) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates, but not 1-chloro-4-nitrobenzene (CNB).1 Publication

Miscellaneous

The use of alternative transcription starts causes dual targeting of the same mature MDAR protein to both mitochondria and chloroplast.1 Publication

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.1 Publication

Cofactori

FADBy similarity

Enzyme regulationi

Redox regulation of the activity by thioredoxin TRXy1.1 Publication

Kineticsi

  1. KM=522 µM for 2,4,6-trinitrotoluene1 Publication
  2. KM=4.1 µM for monodehydroascorbate1 Publication
  3. KM=1254 µM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=0.143 mmol/min/mg enzyme with 2,4,6-trinitrotoluene as substrate1 Publication
  2. Vmax=190 mmol/min/mg enzyme with monodehydroascorbate as substrate1 Publication
  3. Vmax=0.960 mmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei95FADBy similarity1
Binding sitei102FADBy similarity1
Binding sitei107FADBy similarity1
Binding sitei248NADBy similarity1
Binding sitei254NADBy similarity1
Binding sitei254NADPBy similarity1
Binding sitei313NAD; via amide nitrogenBy similarity1
Binding sitei313NADP; via amide nitrogenBy similarity1
Binding sitei351FADBy similarity1
Binding sitei369FAD; via amide nitrogenBy similarity1
Binding sitei373AscorbateBy similarity1
Binding sitei398FAD; via carbonyl oxygenBy similarity1
Binding sitei398NAD; via carbonyl oxygenBy similarity1
Binding sitei398NADP; via carbonyl oxygenBy similarity1
Binding sitei400AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi68 – 71FADBy similarity4
Nucleotide bindingi201 – 202FADBy similarity2
Nucleotide bindingi224 – 230NADBy similarity7
Nucleotide bindingi226 – 230NADPBy similarity5
Nucleotide bindingi367 – 368NADBy similarity2
Nucleotide bindingi367 – 368NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

  • response to cadmium ion Source: TAIR
  • response to cold Source: TAIR

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Enzyme and pathway databases

BioCyciARA:AT1G63940-MONOMER
BRENDAi1.6.5.4 399

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase, chloroplastic/mitochondrial1 Publication (EC:1.6.5.41 Publication)
Alternative name(s):
Monodehydroascorbate reductase 5, mitochondrial1 Publication
Short name:
AtMDAR51 Publication
Monodehydroascorbate reductase 6, chloroplastic1 Publication
Short name:
AtMDAR61 Publication
Gene namesi
Name:MDAR51 Publication
Synonyms:MDAR61 Publication, MDHAR61 Publication, PMDAR-L1 Publication, PMDAR-S1 Publication
Ordered Locus Names:At1g63940Imported
ORF Names:T12P18.4Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G63940
TAIRilocus:2195503 AT1G63940

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Mitochondrion, Plastid

Pathology & Biotechi

Biotechnological usei

The main mechanism for TNT toxicity in plants is the production of superoxide in the mitochondria by MDAR5 with TNT as a substrate. Inactivation of MDAR5 enhance TNT tolerance, thus enabling revegetation and remediation of explosives-contaminated sites.1 Publication

Disruption phenotypei

No visible phenotype, but enhanced 2,4,6-trinitrotoluene (TNT) tolerance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51Chloroplast and mitochondrion1 PublicationAdd BLAST51
ChainiPRO_000001862152 – 493Monodehydroascorbate reductase, chloroplastic/mitochondrialAdd BLAST442

Proteomic databases

PaxDbiP92947
PRIDEiP92947

PTM databases

iPTMnetiP92947

Expressioni

Gene expression databases

ExpressionAtlasiP92947 baseline and differential
GenevisibleiP92947 AT

Interactioni

Subunit structurei

Interacts in vitro with TRXy.1 Publication

Protein-protein interaction databases

BioGridi27918, 2 interactors
IntActiP92947, 2 interactors
STRINGi3702.AT1G63940.2

Structurei

3D structure databases

ProteinModelPortaliP92947
SMRiP92947
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG1336 Eukaryota
COG0446 LUCA
HOGENOMiHOG000276711
InParanoidiP92947
KOiK08232
OMAiSFMAVEA
OrthoDBiEOG093609QX
PhylomeDBiP92947

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform MDAR5 (identifier: P92947-1) [UniParc]FASTAAdd to basket
Also known as: PMDAR-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAVRRVMAL ASTTLPTKSG LSLWCPSSPS LARRFPARFS PIGSRIASRS
60 70 80 90 100
LVTASFANEN REFVIVGGGN AAGYAARTFV ENGMADGRLC IVTKEAYAPY
110 120 130 140 150
ERPALTKAYL FPPEKKPARL PGFHTCVGGG GERQTPDWYK EKGIEVIYED
160 170 180 190 200
PVAGADFEKQ TLTTDAGKQL KYGSLIIATG CTASRFPDKI GGHLPGVHYI
210 220 230 240 250
REVADADSLI ASLGKAKKIV IVGGGYIGME VAAAAVAWNL DTTIVFPEDQ
260 270 280 290 300
LLQRLFTPSL AQKYEELYRQ NGVKFVKGAS INNLEAGSDG RVSAVKLADG
310 320 330 340 350
STIEADTVVI GIGAKPAIGP FETLAMNKSI GGIQVDGLFR TSTPGIFAIG
360 370 380 390 400
DVAAFPLKIY DRMTRVEHVD HARRSAQHCV KSLLTAHTDT YDYLPYFYSR
410 420 430 440 450
VFEYEGSPRK VWWQFFGDNV GETVEVGNFD PKIATFWIES GRLKGVLVES
460 470 480 490
GSPEEFQLLP KLARSQPLVD KAKLASASSV EEALEIAQAA LQS
Length:493
Mass (Da):53,302
Last modified:July 19, 2004 - v3
Checksum:i44FBF0DE65DA89D7
GO
Isoform MDAR6 (identifier: P92947-2) [UniParc]FASTAAdd to basket
Also known as: PMDAR-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-7: Missing.

Show »
Length:486
Mass (Da):52,502
Checksum:i468FBFC7B05EAAAE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti109Y → H in BAA12349 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0113601 – 7Missing in isoform MDAR6. 1 Publication7

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D84417 mRNA Translation: BAA12349.2
AC010852 Genomic DNA Translation: AAG52455.1
CP002684 Genomic DNA Translation: AEE34168.1
CP002684 Genomic DNA Translation: AEE34171.1
AY034934 mRNA Translation: AAK59441.1
AY142572 mRNA Translation: AAN13141.1
BT000667 mRNA Translation: AAN31814.1
PIRiE96664
RefSeqiNP_564818.1, NM_105067.4 [P92947-2]
NP_849839.1, NM_179508.3 [P92947-1]
UniGeneiAt.24374
At.72711

Genome annotation databases

EnsemblPlantsiAT1G63940.1; AT1G63940.1; AT1G63940 [P92947-2]
AT1G63940.2; AT1G63940.2; AT1G63940 [P92947-1]
GeneIDi842697
GrameneiAT1G63940.1; AT1G63940.1; AT1G63940 [P92947-2]
AT1G63940.2; AT1G63940.2; AT1G63940 [P92947-1]
KEGGiath:AT1G63940

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Entry informationi

Entry nameiMDAR_ARATH
AccessioniPrimary (citable) accession number: P92947
Secondary accession number(s): Q94CE2, Q9CAK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: July 19, 2004
Last modified: April 25, 2018
This is version 131 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health