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Protein

Calcium-transporting ATPase 1, endoplasmic reticulum-type

Gene

ECA1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the endoplasmic reticulum lumen. Also regulate manganese ion homeostasis by pumping it into endomembrane compartments. Can also transport zinc.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi336 – 3361Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi337 – 3371Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi339 – 3391Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi341 – 3411Calcium 2By similarity
Active sitei383 – 38314-aspartylphosphate intermediateBy similarity
Metal bindingi731 – 7311MagnesiumBy similarity
Metal bindingi735 – 7351MagnesiumBy similarity
Metal bindingi797 – 7971Calcium 1By similarity
Metal bindingi800 – 8001Calcium 1By similarity
Metal bindingi825 – 8251Calcium 2By similarity
Metal bindingi828 – 8281Calcium 1By similarity
Metal bindingi829 – 8291Calcium 1By similarity
Metal bindingi829 – 8291Calcium 2By similarity
Metal bindingi961 – 9611Calcium 1By similarity

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • calcium ion transport Source: UniProtKB
  • response to cadmium ion Source: TAIR
  • response to manganese ion Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G07810-MONOMER.
MetaCyc:MONOMER-14600.
BRENDAi3.6.3.8. 399.

Protein family/group databases

TCDBi3.A.3.2.13. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 1, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:ECA1
Synonyms:ACA3
Ordered Locus Names:At1g07810
ORF Names:F24B9.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G07810.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070CytoplasmicSequence analysisAdd
BLAST
Transmembranei71 – 9121HelicalSequence analysisAdd
BLAST
Topological domaini92 – 11524LumenalSequence analysisAdd
BLAST
Transmembranei116 – 13520HelicalSequence analysisAdd
BLAST
Topological domaini136 – 278143CytoplasmicSequence analysisAdd
BLAST
Transmembranei279 – 29820HelicalSequence analysisAdd
BLAST
Topological domaini299 – 32729LumenalSequence analysisAdd
BLAST
Transmembranei328 – 34518HelicalSequence analysisAdd
BLAST
Topological domaini346 – 786441CytoplasmicSequence analysisAdd
BLAST
Transmembranei787 – 80620HelicalSequence analysisAdd
BLAST
Topological domaini807 – 81610LumenalSequence analysis
Transmembranei817 – 83721HelicalSequence analysisAdd
BLAST
Topological domaini838 – 85720CytoplasmicSequence analysisAdd
BLAST
Transmembranei858 – 88023HelicalSequence analysisAdd
BLAST
Topological domaini881 – 95070LumenalSequence analysisAdd
BLAST
Transmembranei951 – 97020HelicalSequence analysisAdd
BLAST
Topological domaini971 – 98313CytoplasmicSequence analysisAdd
BLAST
Transmembranei984 – 100219HelicalSequence analysisAdd
BLAST
Topological domaini1003 – 101715LumenalSequence analysisAdd
BLAST
Transmembranei1018 – 103821HelicalSequence analysisAdd
BLAST
Topological domaini1039 – 106123CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • endoplasmic reticulum membrane Source: TAIR
  • integral component of endoplasmic reticulum membrane Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10611061Calcium-transporting ATPase 1, endoplasmic reticulum-typePRO_0000046405Add
BLAST

Proteomic databases

PaxDbiP92939.
PRIDEiP92939.

PTM databases

iPTMnetiP92939.

Expressioni

Gene expression databases

GenevisibleiP92939. AT.

Interactioni

Protein-protein interaction databases

BioGridi22537. 3 interactions.
MINTiMINT-8063382.
STRINGi3702.AT1G07810.1.

Structurei

3D structure databases

ProteinModelPortaliP92939.
SMRiP92939. Positions 70-398, 623-812.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiP92939.
KOiK01537.
OMAiTTYDPFD.
OrthoDBiEOG093600UE.
PhylomeDBiP92939.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92939-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGSEDLVK KESLNSTPVN SDTFPAWAKD VAECEEHFVV SREKGLSSDE
60 70 80 90 100
VLKRHQIYGL NELEKPEGTS IFKLILEQFN DTLVRILLAA AVISFVLAFF
110 120 130 140 150
DGDEGGEMGI TAFVEPLVIF LILIVNAIVG IWQETNAEKA LEALKEIQSQ
160 170 180 190 200
QATVMRDGTK VSSLPAKELV PGDIVELRVG DKVPADMRVV ALISSTLRVE
210 220 230 240 250
QGSLTGESEA VSKTTKHVDE NADIQGKKCM VFAGTTVVNG NCICLVTDTG
260 270 280 290 300
MNTEIGRVHS QIQEAAQHEE DTPLKKKLNE FGEVLTMIIG LICALVWLIN
310 320 330 340 350
VKYFLSWEYV DGWPRNFKFS FEKCTYYFEI AVALAVAAIP EGLPAVITTC
360 370 380 390 400
LALGTRKMAQ KNALVRKLPS VETLGCTTVI CSDKTGTLTT NQMAVSKLVA
410 420 430 440 450
MGSRIGTLRS FNVEGTSFDP RDGKIEDWPM GRMDANLQMI AKIAAICNDA
460 470 480 490 500
NVEQSDQQFV SRGMPTEAAL KVLVEKMGFP EGLNEASSDG DVLRCCRLWS
510 520 530 540 550
ELEQRIATLE FDRDRKSMGV MVDSSSGNKL LLVKGAVENV LERSTHIQLL
560 570 580 590 600
DGSKRELDQY SRDLILQSLR DMSLSALRCL GFAYSDVPSD FATYDGSEDH
610 620 630 640 650
PAHQQLLNPS NYSSIESNLI FVGFVGLRDP PRKEVRQAIA DCRTAGIRVM
660 670 680 690 700
VITGDNKSTA EAICREIGVF EADEDISSRS LTGIEFMDVQ DQKNHLRQTG
710 720 730 740 750
GLLFSRAEPK HKQEIVRLLK EDGEVVAMTG DGVNDAPALK LADIGVAMGI
760 770 780 790 800
SGTEVAKEAS DMVLADDNFS TIVAAVGEGR SIYNNMKAFI RYMISSNIGE
810 820 830 840 850
VASIFLTAAL GIPEGMIPVQ LLWVNLVTDG PPATALGFNP PDKDIMKKPP
860 870 880 890 900
RRSDDSLITA WILFRYMVIG LYVGVATVGV FIIWYTHSSF MGIDLSQDGH
910 920 930 940 950
SLVSYSQLAH WGQCSSWEGF KVSPFTAGSQ TFSFDSNPCD YFQQGKIKAS
960 970 980 990 1000
TLSLSVLVAI EMFNSLNALS EDGSLVTMPP WVNPWLLLAM AVSFGLHFVI
1010 1020 1030 1040 1050
LYVPFLAQVF GIVPLSLNEW LLVLAVSLPV ILIDEVLKFV GRCTSGYRYS
1060
PRTLSTKQKE E
Length:1,061
Mass (Da):116,366
Last modified:January 11, 2001 - v2
Checksum:i4281842216E1340F
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti684 – 6841I → K in strain: cv. Landsberg erecta.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96455 mRNA. Translation: AAC68819.1.
U93845 mRNA. Translation: AAB52420.1.
AC007583 Genomic DNA. Translation: AAF75073.1.
CP002684 Genomic DNA. Translation: AEE28186.1.
AF116533 Genomic DNA. Translation: AAF36087.1.
Y09817 mRNA. Translation: CAA70946.1.
PIRiE86213.
RefSeqiNP_172259.1. NM_100655.3.
UniGeneiAt.25348.
At.26214.

Genome annotation databases

EnsemblPlantsiAT1G07810.1; AT1G07810.1; AT1G07810.
GeneIDi837296.
GrameneiAT1G07810.1; AT1G07810.1; AT1G07810.
KEGGiath:AT1G07810.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96455 mRNA. Translation: AAC68819.1.
U93845 mRNA. Translation: AAB52420.1.
AC007583 Genomic DNA. Translation: AAF75073.1.
CP002684 Genomic DNA. Translation: AEE28186.1.
AF116533 Genomic DNA. Translation: AAF36087.1.
Y09817 mRNA. Translation: CAA70946.1.
PIRiE86213.
RefSeqiNP_172259.1. NM_100655.3.
UniGeneiAt.25348.
At.26214.

3D structure databases

ProteinModelPortaliP92939.
SMRiP92939. Positions 70-398, 623-812.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22537. 3 interactions.
MINTiMINT-8063382.
STRINGi3702.AT1G07810.1.

Protein family/group databases

TCDBi3.A.3.2.13. the p-type atpase (p-atpase) superfamily.

PTM databases

iPTMnetiP92939.

Proteomic databases

PaxDbiP92939.
PRIDEiP92939.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G07810.1; AT1G07810.1; AT1G07810.
GeneIDi837296.
GrameneiAT1G07810.1; AT1G07810.1; AT1G07810.
KEGGiath:AT1G07810.

Organism-specific databases

TAIRiAT1G07810.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
HOGENOMiHOG000265621.
InParanoidiP92939.
KOiK01537.
OMAiTTYDPFD.
OrthoDBiEOG093600UE.
PhylomeDBiP92939.

Enzyme and pathway databases

BioCyciARA:AT1G07810-MONOMER.
MetaCyc:MONOMER-14600.
BRENDAi3.6.3.8. 399.

Miscellaneous databases

PROiP92939.

Gene expression databases

GenevisibleiP92939. AT.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECA1_ARATH
AccessioniPrimary (citable) accession number: P92939
Secondary accession number(s): O04987
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 11, 2001
Last modified: September 7, 2016
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.