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Protein

CBL-interacting serine/threonine-protein kinase 15

Gene

CIPK15

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei41 – 411ATPPROSITE-ProRule annotation
Active sitei134 – 1341Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi18 – 269ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • abscisic acid-activated signaling pathway Source: TAIR
  • negative regulation of abscisic acid-activated signaling pathway Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Manganese, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G01810-MONOMER.
ARA:GQT-1508-MONOMER.
ARA:GQT-759-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
CBL-interacting serine/threonine-protein kinase 15 (EC:2.7.11.1)
Alternative name(s):
SNF1-related kinase 3.1
SOS-interacting protein 2
SOS2-like protein kinase PKS3
Serine/threonine-protein kinase ATPK10
Gene namesi
Name:CIPK15
Synonyms:ATPK10, PKS3, SIP2, SnRK3.1
Ordered Locus Names:At5g01810
ORF Names:T20L15_80
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G01810.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 421421CBL-interacting serine/threonine-protein kinase 15PRO_0000085876Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei156 – 1561PhosphoserineBy similarity
Modified residuei170 – 1701PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP92937.
PRIDEiP92937.

Expressioni

Tissue specificityi

Ubiquitous. Co-expressed with CBL1 in guard cells.3 Publications

Inductioni

Slightly repressed by salt stress.1 Publication

Gene expression databases

ExpressionAtlasiP92937. baseline and differential.
GenevisibleiP92937. AT.

Interactioni

Subunit structurei

Interacts with CBL1/SCaBP5, CBL4/SOS3, ABI1 and ABI2.2 Publications

Protein-protein interaction databases

BioGridi15835. 8 interactions.
IntActiP92937. 2 interactions.
STRINGi3702.AT5G01810.1.

Structurei

3D structure databases

ProteinModelPortaliP92937.
SMRiP92937. Positions 11-407.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini12 – 266255Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini299 – 32325NAFPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni152 – 18130Activation loopBy similarityAdd
BLAST
Regioni328 – 35730PPIBy similarityAdd
BLAST

Domaini

The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases (By similarity).By similarity

Sequence similaritiesi

Contains 1 NAF domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiP92937.
OMAiREASEIT.
OrthoDBiEOG093608Y0.
PhylomeDBiP92937.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P92937-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEKKGSVLML RYEVGKFLGQ GTFAKVYHAR HLKTGDSVAI KVIDKERILK
60 70 80 90 100
VGMTEQIKRE ISAMRLLRHP NIVELHEVMA TKSKIYFVME HVKGGELFNK
110 120 130 140 150
VSTGKLREDV ARKYFQQLVR AVDFCHSRGV CHRDLKPENL LLDEHGNLKI
160 170 180 190 200
SDFGLSALSD SRRQDGLLHT TCGTPAYVAP EVISRNGYDG FKADVWSCGV
210 220 230 240 250
ILFVLLAGYL PFRDSNLMEL YKKIGKAEVK FPNWLAPGAK RLLKRILDPN
260 270 280 290 300
PNTRVSTEKI MKSSWFRKGL QEEVKESVEE ETEVDAEAEG NASAEKEKKR
310 320 330 340 350
CINLNAFEII SLSTGFDLSG LFEKGEEKEE MRFTSNREAS EITEKLVEIG
360 370 380 390 400
KDLKMKVRKK EHEWRVKMSA EATVVEAEVF EIAPSYHMVV LKKSGGDTAE
410 420
YKRVMKESIR PALIDFVLAW H
Length:421
Mass (Da):47,928
Last modified:December 21, 2004 - v2
Checksum:iFE109AA564776B34
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti176 – 1783AYV → TYC (PubMed:7824653).Curated
Sequence conflicti176 – 1783AYV → TYC (PubMed:11230129).Curated
Sequence conflicti176 – 1783AYV → TYC (PubMed:11402167).Curated
Sequence conflicti230 – 2301K → R in BAF00779 (Ref. 6) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30622 mRNA. Translation: BAA06311.1.
AF302111 mRNA. Translation: AAK16692.1.
AF339144 mRNA. Translation: AAK26842.1.
AL162351 Genomic DNA. Translation: CAB82751.1.
CP002688 Genomic DNA. Translation: AED90394.1.
CP002688 Genomic DNA. Translation: AED90395.1.
AK228889 mRNA. Translation: BAF00779.1.
BT028964 mRNA. Translation: ABI54339.1.
PIRiT48202.
RefSeqiNP_001031820.1. NM_001036743.1. [P92937-1]
NP_195801.1. NM_120259.3. [P92937-1]
UniGeneiAt.20284.

Genome annotation databases

EnsemblPlantsiAT5G01810.1; AT5G01810.1; AT5G01810. [P92937-1]
AT5G01810.2; AT5G01810.2; AT5G01810. [P92937-1]
GeneIDi830556.
KEGGiath:AT5G01810.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D30622 mRNA. Translation: BAA06311.1.
AF302111 mRNA. Translation: AAK16692.1.
AF339144 mRNA. Translation: AAK26842.1.
AL162351 Genomic DNA. Translation: CAB82751.1.
CP002688 Genomic DNA. Translation: AED90394.1.
CP002688 Genomic DNA. Translation: AED90395.1.
AK228889 mRNA. Translation: BAF00779.1.
BT028964 mRNA. Translation: ABI54339.1.
PIRiT48202.
RefSeqiNP_001031820.1. NM_001036743.1. [P92937-1]
NP_195801.1. NM_120259.3. [P92937-1]
UniGeneiAt.20284.

3D structure databases

ProteinModelPortaliP92937.
SMRiP92937. Positions 11-407.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi15835. 8 interactions.
IntActiP92937. 2 interactions.
STRINGi3702.AT5G01810.1.

Proteomic databases

PaxDbiP92937.
PRIDEiP92937.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G01810.1; AT5G01810.1; AT5G01810. [P92937-1]
AT5G01810.2; AT5G01810.2; AT5G01810. [P92937-1]
GeneIDi830556.
KEGGiath:AT5G01810.

Organism-specific databases

TAIRiAT5G01810.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233016.
InParanoidiP92937.
OMAiREASEIT.
OrthoDBiEOG093608Y0.
PhylomeDBiP92937.

Enzyme and pathway databases

BioCyciARA:AT5G01810-MONOMER.
ARA:GQT-1508-MONOMER.
ARA:GQT-759-MONOMER.

Miscellaneous databases

PROiP92937.

Gene expression databases

ExpressionAtlasiP92937. baseline and differential.
GenevisibleiP92937. AT.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR018451. NAF/FISL_domain.
IPR004041. NAF_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF03822. NAF. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50816. NAF. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCIPKF_ARATH
AccessioniPrimary (citable) accession number: P92937
Secondary accession number(s): Q0D240, Q0WQ20, Q9LZW5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.