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Protein

Chlorophyll a-b binding protein, chloroplastic

Gene

LHC0

Organism
Apium graveolens (Celery)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity
Binding sitei78 – 781Chlorophyll-a 1; via amide nitrogenBy similarity
Binding sitei84 – 841Chlorophyll-a 1By similarity
Metal bindingi97 – 971Magnesium (chlorophyll-a 1 axial ligand)By similarity
Metal bindingi100 – 1001Magnesium (chlorophyll-a 2 axial ligand)By similarity
Binding sitei102 – 1021Chlorophyll-b 2By similarity
Binding sitei135 – 1351Chlorophyll-a 3By similarity
Binding sitei145 – 1451Chlorophyll-a 3; via amide nitrogenBy similarity
Metal bindingi151 – 1511Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity
Binding sitei155 – 1551Chlorophyll-b 3By similarity
Binding sitei163 – 1631Chlorophyll-b 4 or chlorophyll-b 5By similarity
Metal bindingi171 – 1711Magnesium (chlorophyll-b 3 axial ligand)By similarity
Binding sitei174 – 1741Chlorophyll-b 4By similarity
Binding sitei180 – 1801Chlorophyll-b 2; via amide nitrogenBy similarity
Binding sitei211 – 2111Chlorophyll-a 5By similarity
Metal bindingi212 – 2121Magnesium (chlorophyll-a 3 axial ligand)By similarity
Metal bindingi215 – 2151Magnesium (chlorophyll-a 4 axial ligand)By similarity
Binding sitei217 – 2171Chlorophyll-a 1By similarity
Metal bindingi229 – 2291Magnesium (chlorophyll-a 5 axial ligand)By similarity
Metal bindingi244 – 2441Magnesium (chlorophyll-a 6 axial ligand)By similarity
Binding sitei253 – 2531Chlorophyll-a 6; via amide nitrogenBy similarity
Binding sitei260 – 2601Chlorophyll-b 5; via carbonyl oxygenBy similarity

GO - Molecular functioni

  1. chlorophyll binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. photosynthesis, light harvesting Source: InterPro
  2. protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein, chloroplastic
Alternative name(s):
Allergen: Api g 3
Gene namesi
Name:LHC0
OrganismiApium graveolens (Celery)
Taxonomic identifieri4045 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeapioid supercladeApieaeApium

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei98 – 11821HelicalSequence AnalysisAdd
BLAST
Transmembranei150 – 17021HelicalSequence AnalysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. photosystem I Source: UniProtKB-KW
  4. photosystem II Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Photosystem II, Plastid, Thylakoid

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3086. Api g 3.0101.
774. Api g 3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535ChloroplastSequence AnalysisAdd
BLAST
Chaini36 – 264229Chlorophyll a-b binding protein, chloroplasticPRO_0000003646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei36 – 361N2-acetylarginineBy similarity
Modified residuei38 – 381PhosphothreonineBy similarity

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP92919.

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins.

Structurei

3D structure databases

ProteinModelPortaliP92919.
SMRiP92919. Positions 46-264.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P92919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASTMALSS PALAGKAVKV APSSSELFGN GRVSMRKTVK APVSDSPWYG
60 70 80 90 100
PDRVKYLGPF SGEAPSYLTG EFPGDYGWDT AGLSADPETF AKNRELEVIH
110 120 130 140 150
SRWAMLGALG CVFPELLARN GVKFGEAVWF KAGSQIFSEG GLDYLGNPSL
160 170 180 190 200
VHAQSILSIW ATQVILMGAV EGYRVAGGPL GEIVDPLYPG GSFDPLGLAE
210 220 230 240 250
DPERSAELKV KELKNGRLAM FSMFGFFVQA IVTGKGPLEN LADHLADPVN
260
NNAWAFATNF VPGK
Length:264
Mass (Da):28,076
Last modified:May 1, 1997 - v1
Checksum:i07126C7D5D86BA66
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75663 mRNA. Translation: CAA99993.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z75663 mRNA. Translation: CAA99993.1.

3D structure databases

ProteinModelPortaliP92919.
SMRiP92919. Positions 46-264.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3086. Api g 3.0101.
774. Api g 3.

Proteomic databases

PRIDEiP92919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Characterization of a chlorophyll ab binding protein from celery as a food allergen Api g 3."
    Hoffmann-Sommergruber K., Pec M., Ferris R., O'Riordain G., Ebner C., Scheiner O., Breiteneder H.
    Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bulb.

Entry informationi

Entry nameiCB23_APIGR
AccessioniPrimary (citable) accession number: P92919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.