Reviewed, UniProtKB/Swiss-Prot P92919 (CB23_APIGR)

Names and origin

Protein names

Recommended name:
    Chlorophyll a-b binding protein, chloroplastic
Alternative name(s):
    Allergen=Api g 3

Gene names

Name:

LHC0

Organism

Apium graveolens (Celery)

Taxonomic identifier

4045 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Apiales › Apiaceae › Apioideae › apioid superclade › Apium clade › Apium

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.
Cofactor	Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin By similarity.
Subunit structure	The LHC complex consists of chlorophyll a-b binding proteins.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein.
Domain	The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.
Post-translational modification	Photoregulated by reversible phosphorylation of its threonine residues By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the light-harvesting chlorophyll a/b-binding (LHC) protein family.

Ontologies

Keywords
Biological process	Photosynthesis
Cellular component	Chloroplast Membrane Photosystem I Photosystem II Plastid Thylakoid
Disease	Allergen
Domain	Transit peptide Transmembrane
Ligand	Chlorophyll Chromophore Magnesium Metal-binding
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	photosynthesis, light harvesting Inferred from electronic annotation. Source: InterPro protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell photosystem I Inferred from electronic annotation. Source: UniProtKB-KW photosystem II Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chlorophyll binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 35	35	Chloroplast Potential
	Chain	36 – 264	229	Chlorophyll a-b binding protein, chloroplastic		PRO_0000003646
Regions
	Transmembrane	98 – 118	21	Potential
	Transmembrane	150 – 170	21	Potential
	Transmembrane	218 – 238	21	Potential
Sites
	Metal binding	56	1	Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygen By similarity
	Metal binding	97	1	Magnesium (chlorophyll-a 1 axial ligand) By similarity
	Metal binding	100	1	Magnesium (chlorophyll-a 2 axial ligand) By similarity
	Metal binding	151	1	Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygen By similarity
	Metal binding	171	1	Magnesium (chlorophyll-b 3 axial ligand) By similarity
	Metal binding	212	1	Magnesium (chlorophyll-a 3 axial ligand) By similarity
	Metal binding	215	1	Magnesium (chlorophyll-a 4 axial ligand) By similarity
	Metal binding	229	1	Magnesium (chlorophyll-a 5 axial ligand) By similarity
	Metal binding	244	1	Magnesium (chlorophyll-a 6 axial ligand) By similarity
	Binding site	78	1	Chlorophyll-a 1; via amide nitrogen By similarity
	Binding site	84	1	Chlorophyll-a 1 By similarity
	Binding site	102	1	Chlorophyll-b 2 By similarity
	Binding site	135	1	Chlorophyll-a 3 By similarity
	Binding site	145	1	Chlorophyll-a 3; via amide nitrogen By similarity
	Binding site	155	1	Chlorophyll-b 3 By similarity
	Binding site	163	1	Chlorophyll-b 4 or chlorophyll-b 5 By similarity
	Binding site	174	1	Chlorophyll-b 4 By similarity
	Binding site	180	1	Chlorophyll-b 2; via amide nitrogen By similarity
	Binding site	211	1	Chlorophyll-a 5 By similarity
	Binding site	217	1	Chlorophyll-a 1 By similarity
	Binding site	253	1	Chlorophyll-a 6; via amide nitrogen By similarity
	Binding site	260	1	Chlorophyll-b 5; via carbonyl oxygen By similarity
Amino acid modifications
	Modified residue	36	1	N2-acetylarginine By similarity
	Modified residue	38	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P92919-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 07126C7D5D86BA66	FASTA	264	28,076
	10 20 30 40 50 60 MAASTMALSS PALAGKAVKV APSSSELFGN GRVSMRKTVK APVSDSPWYG PDRVKYLGPF 70 80 90 100 110 120 SGEAPSYLTG EFPGDYGWDT AGLSADPETF AKNRELEVIH SRWAMLGALG CVFPELLARN 130 140 150 160 170 180 GVKFGEAVWF KAGSQIFSEG GLDYLGNPSL VHAQSILSIW ATQVILMGAV EGYRVAGGPL 190 200 210 220 230 240 GEIVDPLYPG GSFDPLGLAE DPERSAELKV KELKNGRLAM FSMFGFFVQA IVTGKGPLEN 250 260 LADHLADPVN NNAWAFATNF VPGK

References

[1]

"Characterization of a chlorophyll ab binding protein from celery as a food allergen Api g 3." Hoffmann-Sommergruber K., Pec M., Ferris R., O'Riordain G., Ebner C., Scheiner O., Breiteneder H. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Bulb.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z75663 mRNA. Translation: CAA99993.1.
3D structure databases
SMR	P92919. Positions 46-264.
ModBase	Search...
Family and domain databases
InterPro	IPR001344. Chloro_AB_bd. [Graphical view]
PANTHER	PTHR21649. Chloro_AB_bd. 1 hit.
Pfam	PF00504. Chloroa_b-bind. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CB23_APIGR

Accession

Primary (citable) accession number: P92919

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 1997
Last modified:	November 24, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)