ID COX3_ARATH Reviewed; 265 AA. AC P92514; A0A2P2CLH8; A7KNG3; F4IMA5; Q8S8B5; DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot. DT 04-MAY-2001, sequence version 2. DT 24-JAN-2024, entry version 166. DE RecName: Full=Cytochrome c oxidase subunit 3; DE EC=7.1.1.9; DE AltName: Full=Cytochrome c oxidase polypeptide III; GN Name=COX3; GN OrderedLocusNames=AtMg00730 {ECO:0000312|Araport:ATMG00730}; GN and GN OrderedLocusNames=At2g07687 {ECO:0000312|Araport:AT2G07687}; OS Arabidopsis thaliana (Mouse-ear cress). OG Mitochondrion. OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. OX NCBI_TaxID=3702; RN [1] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. C24; RX PubMed=8988169; DOI=10.1038/ng0197-57; RA Unseld M., Marienfeld J.R., Brandt P., Brennicke A.; RT "The mitochondrial genome of Arabidopsis thaliana contains 57 genes in RT 366,924 nucleotides."; RL Nat. Genet. 15:57-61(1997). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=cv. C24, cv. Columbia, and cv. Landsberg erecta; RX PubMed=21951689; DOI=10.1186/1741-7007-9-64; RA Davila J.I., Arrieta-Montiel M.P., Wamboldt Y., Cao J., Hagmann J., RA Shedge V., Xu Y.Z., Weigel D., Mackenzie S.A.; RT "Double-strand break repair processes drive evolution of the mitochondrial RT genome in Arabidopsis."; RL BMC Biol. 9:64-64(2011). RN [3] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND RNA EDITING. RC STRAIN=cv. Columbia; RX PubMed=29519893; DOI=10.1105/tpc.18.00024; RA Sloan D.B., Wu Z., Sharbrough J.; RT "Correction of persistent errors in Arabidopsis reference mitochondrial RT genomes."; RL Plant Cell 30:525-527(2018). RN [4] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07687). RC STRAIN=cv. Columbia; RX PubMed=10617197; DOI=10.1038/45471; RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.; RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."; RL Nature 402:761-768(1999). RN [5] RP GENOME REANNOTATION (AT2G07687). RC STRAIN=cv. Columbia; RX PubMed=27862469; DOI=10.1111/tpj.13415; RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S., RA Town C.D.; RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference RT genome."; RL Plant J. 89:789-804(2017). RN [6] RP NUCLEOTIDE SEQUENCE [MRNA] OF 12-258, AND RNA EDITING. RC STRAIN=cv. Columbia, and cv. Landsberg erecta; TISSUE=Rosette leaf; RX PubMed=17565941; DOI=10.1534/genetics.107.073585; RA Bentolila S., Elliott L.E., Hanson M.R.; RT "Genetic architecture of mitochondrial editing in Arabidopsis thaliana."; RL Genetics 178:1693-1708(2008). RN [7] RP RNA EDITING. RX PubMed=10611383; DOI=10.1073/pnas.96.26.15324; RA Giege P., Brennicke A.; RT "RNA editing in Arabidopsis mitochondria effects 441 C to U changes in RT ORFs."; RL Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999). CC -!- FUNCTION: Component of the cytochrome c oxidase, the last enzyme in the CC mitochondrial electron transport chain which drives oxidative CC phosphorylation. The respiratory chain contains 3 multisubunit CC complexes succinate dehydrogenase (complex II, CII), ubiquinol- CC cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CC CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to CC transfer electrons derived from NADH and succinate to molecular oxygen, CC creating an electrochemical gradient over the inner membrane that CC drives transmembrane transport and the ATP synthase. Cytochrome c CC oxidase is the component of the respiratory chain that catalyzes the CC reduction of oxygen to water. Electrons originating from reduced CC cytochrome c in the intermembrane space (IMS) are transferred via the CC dinuclear copper A center (CU(A)) of subunit 2 and heme A of subunit 1 CC to the active site in subunit 1, a binuclear center (BNC) formed by CC heme A3 and copper B (CU(B)). The BNC reduces molecular oxygen to 2 CC water molecules using 4 electrons from cytochrome c in the IMS and 4 CC protons from the mitochondrial matrix. {ECO:0000250|UniProtKB:P00420}. CC -!- CATALYTIC ACTIVITY: CC Reaction=4 Fe(II)-[cytochrome c] + 8 H(+)(in) + O2 = 4 Fe(III)- CC [cytochrome c] + 4 H(+)(out) + 2 H2O; Xref=Rhea:RHEA:11436, CC Rhea:RHEA-COMP:10350, Rhea:RHEA-COMP:14399, ChEBI:CHEBI:15377, CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:29033, CC ChEBI:CHEBI:29034; EC=7.1.1.9; CC Evidence={ECO:0000250|UniProtKB:P00420}; CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11437; CC Evidence={ECO:0000250|UniProtKB:P00420}; CC -!- SUBUNIT: Component of the cytochrome c oxidase (complex IV, CIV), a CC multisubunit enzyme composed of a catalytic core of 3 subunits and CC several supernumerary subunits. The complex exists as a monomer or a CC dimer and forms supercomplexes (SCs) in the inner mitochondrial CC membrane with ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 CC complex, complex III, CIII). {ECO:0000250|UniProtKB:P00420}. CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane CC {ECO:0000250|UniProtKB:P00420}; Multi-pass membrane protein CC {ECO:0000250|UniProtKB:P00420}. CC -!- RNA EDITING: Modified_positions=38 {ECO:0000269|PubMed:10611383, CC ECO:0000269|PubMed:17565941, ECO:0000269|PubMed:29519893}, 82 CC {ECO:0000269|PubMed:10611383, ECO:0000269|PubMed:17565941, CC ECO:0000269|PubMed:29519893}, 86 {ECO:0000269|PubMed:10611383, CC ECO:0000269|PubMed:17565941, ECO:0000269|PubMed:29519893}, 104 CC {ECO:0000269|PubMed:10611383, ECO:0000269|PubMed:17565941, CC ECO:0000269|PubMed:29519893}, 105 {ECO:0000269|PubMed:10611383, CC ECO:0000269|PubMed:17565941, ECO:0000269|PubMed:29519893}, 138 CC {ECO:0000269|PubMed:10611383, ECO:0000269|PubMed:17565941, CC ECO:0000269|PubMed:29519893}, 141 {ECO:0000269|PubMed:10611383, CC ECO:0000269|PubMed:17565941, ECO:0000269|PubMed:29519893}; CC -!- MISCELLANEOUS: A stretch of 270 kb of the mitochondrial genome is CC duplicated within the centromere of chromosome 2 resulting in the CC duplication of the gene. The expression of this duplicated gene CC (At2g07687) is not demonstrated. It is also probably not RNA edited and CC therefore differs in all the positions known to be edited. CC -!- SIMILARITY: Belongs to the cytochrome c oxidase subunit 3 family. CC {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; Y08501; CAA69818.3; ALT_SEQ; Genomic_DNA. DR EMBL; JF729200; AEK01262.1; ALT_SEQ; Genomic_DNA. DR EMBL; JF729201; AEK01298.1; ALT_SEQ; Genomic_DNA. DR EMBL; JF729202; AEK01326.1; ALT_SEQ; Genomic_DNA. DR EMBL; BK010421; DAB41522.2; -; Genomic_DNA. DR EMBL; AC007143; AAM15412.1; ALT_SEQ; Genomic_DNA. DR EMBL; CP002685; AEC06078.1; ALT_SEQ; Genomic_DNA. DR EMBL; EF488906; ABS50618.1; -; mRNA. DR EMBL; EF488907; ABS50619.1; -; mRNA. DR RefSeq; NP_085532.2; NC_001284.2. DR RefSeq; NP_178782.1; NM_126741.2. DR AlphaFoldDB; P92514; -. DR SMR; P92514; -. DR STRING; 3702.A0A2P2CLH8; -. DR PaxDb; 3702-AT2G07687-1; -. DR GeneID; 815364; -. DR KEGG; ath:AT2G07687; -. DR Araport; AT2G07687; -. DR Araport; ATMG00730; -. DR TAIR; AT2G07687; -. DR TAIR; ATMG00730; COX3. DR eggNOG; KOG4664; Eukaryota. DR HOGENOM; CLU_044071_0_0_1; -. DR InParanoid; P92514; -. DR OrthoDB; 984876at2759; -. DR PhylomeDB; P92514; -. DR BioCyc; ARA:AT2G07687-MONOMER; -. DR BioCyc; ARA:ATMG00730-MONOMER; -. DR BioCyc; MetaCyc:ATMG00730-MONOMER; -. DR PRO; PR:P92514; -. DR Proteomes; UP000006548; Chromosome 2. DR Proteomes; UP000006548; Mitochondrion MT. DR ExpressionAtlas; P92514; baseline and differential. DR GO; GO:0005743; C:mitochondrial inner membrane; IEA:UniProtKB-SubCell. DR GO; GO:0004129; F:cytochrome-c oxidase activity; IEA:UniProtKB-EC. DR GO; GO:0006123; P:mitochondrial electron transport, cytochrome c to oxygen; IBA:GO_Central. DR CDD; cd01665; Cyt_c_Oxidase_III; 1. DR Gene3D; 1.10.287.70; -; 1. DR Gene3D; 1.20.120.80; Cytochrome c oxidase, subunit III, four-helix bundle; 1. DR InterPro; IPR024791; Cyt_c/ubiquinol_Oxase_su3. DR InterPro; IPR033945; Cyt_c_oxase_su3_dom. DR InterPro; IPR000298; Cyt_c_oxidase-like_su3. DR InterPro; IPR035973; Cyt_c_oxidase_su3-like_sf. DR InterPro; IPR013833; Cyt_c_oxidase_su3_a-hlx. DR PANTHER; PTHR11403:SF7; CYTOCHROME C OXIDASE SUBUNIT 3; 1. DR PANTHER; PTHR11403; CYTOCHROME C OXIDASE SUBUNIT III; 1. DR Pfam; PF00510; COX3; 1. DR SUPFAM; SSF81452; Cytochrome c oxidase subunit III-like; 1. DR PROSITE; PS50253; COX3; 1. DR Genevisible; P92514; AT. PE 2: Evidence at transcript level; KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome; KW RNA editing; Translocase; Transmembrane; Transmembrane helix. FT CHAIN 1..265 FT /note="Cytochrome c oxidase subunit 3" FT /id="PRO_0000183737" FT TRANSMEM 41..61 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 85..105 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 137..157 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 162..182 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 200..220 FT /note="Helical" FT /evidence="ECO:0000255" FT TRANSMEM 245..265 FT /note="Helical" FT /evidence="ECO:0000255" SQ SEQUENCE 265 AA; 29961 MW; 753978DE1CAA13F1 CRC64; MIESQRHSYH LVDPSPWPIS GSLGALATTV GGVMYMHSFQ GGARLLSLGL IFILYTMFVW WRDVLRESTL EGHHTKVVQL GLRYGFILFI VSEVMFFFAF FWAFFHSSLA PAVEIGGIWP PKGIEVLDPW EIPFLNTLIL LSSGAAVTWA HHAILAGKEK RAVYALVATV LLALVFTGFQ GMEYYQAPFT ISDSIYGSTF FLATGFHGFH VIIGTLFLII CGIRQYLGHL TKEHHVGFEA AAWYWHFVDV VWLFLFVSIY WWGGI //