P92514 (COX3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c oxidase subunit 3
EC=1.9.3.1

Alternative name(s):
Cytochrome c oxidase polypeptide III

Gene names

Name:	COX3
Ordered Locus Names:	AtMg00730

Encoded on

Mitochondrion

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	265 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Subunits I, II and III form the functional core of the enzyme complex.
Catalytic activity	4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity.
Miscellaneous	A stretch of 270 kb of the mitochondrial genome is duplicated within the centromere of chromosome 2 resulting in the duplication of the gene. The expression of this duplicated gene (At2g07687) is not demonstrated. It is also probably not RNA edited and therefore differs in all the positions known to be edited.
Sequence similarities	Belongs to the cytochrome c oxidase subunit 3 family.
RNA editing	Edited at positions 38, 82, 86, 104, 105, 138 and 141. Ref.6

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Coding sequence diversity	RNA editing
Domain	Transmembrane Transmembrane helix
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aerobic electron transport chain Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial respiratory chain complex IV Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	cytochrome-c oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 265	265	Cytochrome c oxidase subunit 3		PRO_0000183737
Regions
	Transmembrane	41 – 61	21	Helical; Potential
	Transmembrane	85 – 105	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Transmembrane	162 – 182	21	Helical; Potential
	Transmembrane	200 – 220	21	Helical; Potential
	Transmembrane	245 – 265	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P92514 [UniParc]. Last modified May 4, 2001. Version 2. Checksum: 753978DE1CAA13F1	FASTA	265	29,961
	10 20 30 40 50 60 MIESQRHSYH LVDPSPWPIS GSLGALATTV GGVMYMHSFQ GGARLLSLGL IFILYTMFVW 70 80 90 100 110 120 WRDVLRESTL EGHHTKVVQL GLRYGFILFI VSEVMFFFAF FWAFFHSSLA PAVEIGGIWP 130 140 150 160 170 180 PKGIEVLDPW EIPFLNTLIL LSSGAAVTWA HHAILAGKEK RAVYALVATV LLALVFTGFQ 190 200 210 220 230 240 GMEYYQAPFT ISDSIYGSTF FLATGFHGFH VIIGTLFLII CGIRQYLGHL TKEHHVGFEA 250 260 AAWYWHFVDV VWLFLFVSIY WWGGI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides." Unseld M., Marienfeld J.R., Brandt P., Brennicke A. Nat. Genet. 15:57-61(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Double-strand break repair processes drive evolution of the mitochondrial genome in Arabidopsis." Davila J.I., Arrieta-Montiel M.P., Wamboldt Y., Cao J., Hagmann J., Shedge V., Xu Y.Z., Weigel D., Mackenzie S.A. BMC Biol. 9:64-64(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. C24, cv. Columbia and cv. Landsberg erecta.
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (AT2G07687). Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION (AT2G07687). Strain: cv. Columbia.
[5]	"Genetic architecture of mitochondrial editing in Arabidopsis thaliana." Bentolila S., Elliott L.E., Hanson M.R. Genetics 178:1693-1708(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-258, RNA-EDITING. Strain: cv. Columbia and cv. Landsberg erecta. Tissue: Rosette leaf.
[6]	"RNA editing in Arabidopsis mitochondria effects 441 C to U changes in ORFs." Giege P., Brennicke A. Proc. Natl. Acad. Sci. U.S.A. 96:15324-15329(1999) [PubMed] [Europe PMC] [Abstract] Cited for: RNA EDITING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y08501 Genomic DNA. Translation: CAA69818.3. Sequence problems. JF729200 Genomic DNA. Translation: AEK01262.1. Sequence problems. JF729201 Genomic DNA. Translation: AEK01298.1. Sequence problems. JF729202 Genomic DNA. Translation: AEK01326.1. Sequence problems. AC007143 Genomic DNA. Translation: AAM15412.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC06078.1. Sequence problems. EF488906 mRNA. Translation: ABS50618.1. EF488907 mRNA. Translation: ABS50619.1.
IPI	IPI00532800. IPI01020420.
RefSeq	NP_085532.2. NC_001284.2. NP_178782.1. NM_126741.2.
UniGene	At.47771.
3D structure databases
ProteinModelPortal	P92514.
SMR	P92514. Positions 5-263.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.ATMG00730.1-P.
Proteomic databases
PaxDb	P92514.
PRIDE	P92514.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	814601. 815364.
KEGG	ath:ArthMp065. ath:AT2G07687.
Organism-specific databases
TAIR	AtMg00730.
Phylogenomic databases
eggNOG	COG1845.
HOGENOM	HOG000264954.
InParanoid	P92514.
KO	K02262.
PhylomeDB	P92514.
ProtClustDB	PLN02194.
Enzyme and pathway databases
BioCyc	ARA:ATMG00730-MONOMER. MetaCyc:ATMG00730-MONOMER.
Gene expression databases
ArrayExpress	P92514.
Genevestigator	P92514.
GermOnline	ATMG00730. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.120.80. 1 hit.
InterPro	IPR024791. Cyt_c/ubiquinol_Oxase_su3. IPR000298. Cyt_c_oxidase_su3. IPR013833. Cyt_c_oxidase_su3_a-hlx. [Graphical view]
PANTHER	PTHR11403. PTHR11403. 1 hit.
Pfam	PF00510. COX3. 1 hit. [Graphical view]
SUPFAM	SSF81452. CytC_oxdse_III. 1 hit.
PROSITE	PS50253. COX3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

COX3_ARATH

Accession

Primary (citable) accession number: P92514
Secondary accession number(s): A7KNG3, F4IMA5, Q8S8B5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 4, 2001
Last modified:	May 1, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program