Reviewed, UniProtKB/Swiss-Prot P92432 (NU1C_HORVU)

Names and origin

Protein names

Recommended name:
    NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic
    EC=1.6.5.-
Alternative name(s):
    NAD(P)H dehydrogenase subunit 1
      Short name=NDH subunit 1
    NADH-plastoquinone oxidoreductase subunit 1

Gene names

Name:

ndhA

Encoded on

Plastid; Chloroplast

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NAD(P)H + plastoquinone = NAD(P)+ + plastoquinol. HAMAP MF_01350
Subunit structure	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus By similarity.
Subcellular location	Plastid › chloroplast thylakoid membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the complex I subunit 1 family.
RNA editing	Edited at positions 17, 158, 188 and 357. Ref.1

Ontologies

Keywords
Cellular component	Chloroplast Membrane Plastid Thylakoid
Coding sequence diversity	RNA editing
Domain	Transmembrane
Ligand	NAD NADP Plastoquinone
Molecular function	Oxidoreductase
PTM	Quinone
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic HAMAP MF_01350		PRO_0000240021
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	103 – 123	21	Potential
	Transmembrane	128 – 148	21	Potential
	Transmembrane	164 – 184	21	Potential
	Transmembrane	202 – 222	21	Potential
	Transmembrane	247 – 267	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	342 – 362	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P92432-1 [UniParc]. Last modified June 13, 2006. Version 4. Checksum: 4DBEE96146BF6154	FASTA	362	40,373
	10 20 30 40 50 60 MIIDRVEVET INSFSKLELL KEVYGLISIL PILTLLLGIT IEVLVIVWLE REISASIQQR 70 80 90 100 110 120 IGPEYAGPLG LLQAIADGTK LLFKEDILPS RGDISLFSIG PSIAVISVLL SFLVIPLGYH 130 140 150 160 170 180 FVLADLSIGV FLWIAISSIA PIGLLMAGYS SNNKYSFLGG LRAAAQSISY EIPLTFCVLA 190 200 210 220 230 240 ISLLSNSLST VDIVEAQSKY GFFGWNIWRQ PIGFLVFLIS SLAECERLPF DLPEAEEELV 250 260 270 280 290 300 AGYQTEYSGI KYGLFYLVSY LNLLVSSLFV TVLYLGGWNF SIPYISFFDF FQMNKAVGIL 310 320 330 340 350 360 EMTMGIFITL TKAYLFLFIS ITIRWTLPRM RMDQLLNLGW KFLLPISLGN LLLTTSFQLV SL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transcripts of the ndhH-D operon of barley plastids: possible role of unedited site III in splicing of the ndhA intron." del Campo E.M., Sabater B., Martin M. Nucleic Acids Res. 28:1092-1098(2000) [PubMed: 10666448] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], RNA EDITING. Strain: cv. Hassan. Tissue: Leaf.
[2]	"Complete chloroplast genome sequences of Hordeum vulgare, Sorghum bicolor and Agrostis stolonifera, and comparative analyses with other grass genomes." Saski C., Lee S.-B., Fjellheim S., Guda C., Jansen R.K., Luo H., Tomkins J., Rognli O.A., Daniell H., Clarke J.L. Theor. Appl. Genet. 115:571-590(2007) [PubMed: 17534593] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Morex.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ011848 Genomic DNA. Translation: CAA09812.1. Sequence problems. EF115541 Genomic DNA. Translation: ABK79467.1. Sequence problems.
RefSeq	YP_874707.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4525172.
Organism-specific databases
Gramene	P92432.
Family and domain databases
HAMAP	MF_01350. [Tree]
InterPro	IPR001694. NADH_UbQ_OxRdtase_su1. IPR018086. NADH_UbQ_OxRdtase_su1_CS. [Graphical view]
PANTHER	PTHR11432. Resp_NADH_DH_1. 1 hit.
Pfam	PF00146. NADHdh. 1 hit. [Graphical view]
PROSITE	PS00667. COMPLEX1_ND1_1. 1 hit. PS00668. COMPLEX1_ND1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NU1C_HORVU

Accession

Primary (citable) accession number: P92432
Secondary accession number(s): A1E9P5, O20233

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	June 13, 2006
Last modified:	June 16, 2009
This is version 49 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)