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P92208

- JNK_DROME

UniProt

P92208 - JNK_DROME

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Protein

Stress-activated protein kinase JNK

Gene

bsk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Responds to activation by environmental stress by phosphorylating a number of transcription factors, primarily components of AP-1 such as Jra and also the transcriptional repressor aop, and thus regulates transcriptional activity. Component of the immune response activated by bacterial infection, and is involved in wound healing and in dorsal closure, a morphogenetic movement during embryogenesis. Controls the expression of a phosphatase, puckered, at the edges of wounded epidermal tissue and in the dorsal epithelium during dorsal closure.4 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium.By similarity

Enzyme regulationi

Activated by threonine and tyrosine phosphorylation by the dual specificity kinase, hep. Inhibited by dual specificity phosphatase, puckered.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATPPROSITE-ProRule annotation
Active sitei149 – 1491Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi31 – 366ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. JUN kinase activity Source: UniProtKB
  3. protein kinase activity Source: FlyBase
  4. protein serine/threonine kinase activity Source: FlyBase

GO - Biological processi

  1. antibacterial humoral response Source: FlyBase
  2. axon extension Source: FlyBase
  3. axon guidance Source: FlyBase
  4. border follicle cell migration Source: FlyBase
  5. cellular response to arsenic-containing substance Source: FlyBase
  6. cellular response to cadmium ion Source: FlyBase
  7. cellular response to oxidative stress Source: FlyBase
  8. cellular response to reactive oxygen species Source: FlyBase
  9. collateral sprouting of injured axon Source: FlyBase
  10. determination of digestive tract left/right asymmetry Source: FlyBase
  11. dorsal appendage formation Source: FlyBase
  12. dorsal closure Source: FlyBase
  13. embryonic anterior midgut (ectodermal) morphogenesis Source: FlyBase
  14. engulfment of apoptotic cell Source: FlyBase
  15. establishment of planar polarity Source: FlyBase
  16. imaginal disc-derived male genitalia morphogenesis Source: FlyBase
  17. imaginal disc fusion, thorax closure Source: FlyBase
  18. JNK cascade Source: UniProtKB
  19. JUN phosphorylation Source: FlyBase
  20. MAPK cascade Source: FlyBase
  21. melanization defense response Source: FlyBase
  22. micropyle formation Source: FlyBase
  23. negative regulation of JUN kinase activity Source: FlyBase
  24. neuron development Source: FlyBase
  25. neuron projection morphogenesis Source: FlyBase
  26. ovarian follicle cell development Source: FlyBase
  27. positive regulation of autophagy Source: FlyBase
  28. protein phosphorylation Source: FlyBase
  29. response to heat Source: FlyBase
  30. response to oxidative stress Source: FlyBase
  31. Wnt signaling pathway Source: FlyBase
  32. wound healing Source: UniProtKB
  33. wound healing, spreading of epidermal cells Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 1994.
ReactomeiREACT_181694. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_181712. Oxidative Stress Induced Senescence.
REACT_202759. FCERI mediated MAPK activation.
REACT_34513. DSCAM interactions.
SignaLinkiP92208.

Names & Taxonomyi

Protein namesi
Recommended name:
Stress-activated protein kinase JNK (EC:2.7.11.24)
Short name:
dJNK
Alternative name(s):
Protein basket
Gene namesi
Name:bsk
Synonyms:JNK
ORF Names:CG5680
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000229. bsk.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. axon Source: FlyBase
  2. cytoplasm Source: UniProtKB-KW
  3. dendrite Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi225 – 2251G → E in BSK-1; defect in dorsal closure. 1 Publication
Mutagenesisi316 – 37257Missing in BSK-2; defect in dorsal closure. 1 PublicationAdd
BLAST

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Stress-activated protein kinase JNKPRO_0000186271Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei181 – 1811PhosphothreonineBy similarity
Modified residuei183 – 1831PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-181 and Tyr-183, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP92208.
PRIDEiP92208.

Expressioni

Tissue specificityi

During gastrulation, expression is seen in cells undergoing morphogenetic movements. By stage 9 of embryonic development, expression is ubiquitous. At stages 12-14, expression occurs in epidermis and central nervous system. At stage 15, expression is restricted to ventral nerve cord, brain and some peripheral neurons. In larvae, expression is seen in all imaginal disks, with highest levels in wing and eye disks, and in the CNS. Adults express the protein in fat body and hemocytes.2 Publications

Developmental stagei

Expressed maternally and zygotically through to adult (male and female).1 Publication

Gene expression databases

BgeeiP92208.
ExpressionAtlasiP92208. differential.

Interactioni

Protein-protein interaction databases

BioGridi69288. 49 interactions.
DIPiDIP-17307N.
IntActiP92208. 18 interactions.
MINTiMINT-337077.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4M3AX-ray1.96A1-372[»]
ProteinModelPortaliP92208.
SMRiP92208. Positions 9-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 320297Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi181 – 1833TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
InParanoidiP92208.
KOiK04440.
OMAiEVMNFEE.
OrthoDBiEOG7PCJGV.
PhylomeDBiP92208.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01772. JNKMAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P92208-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTAQHQHYT VEVGDTNFTI HSRYINLRPI GSGAQGIVCA AYDTITQQNV
60 70 80 90 100
AIKKLSRPFQ NVTHAKRAYR EFKLMKLVNH KNIIGLLNAF TPQRNLEEFQ
110 120 130 140 150
DVYLVMELMD ANLCQVIQMD LDHDRMSYLL YQMLCGIKHL HSAGIIHRDL
160 170 180 190 200
KPSNIVVKAD CTLKILDFGL ARTAGTTFMM TPYVVTRYYR APEVILGMGY
210 220 230 240 250
TENVDIWSVG CIMGEMIRGG VLFPGTDHID QWNKIIEQLG TPSPSFMQRL
260 270 280 290 300
QPTVRNYVEN RPRYTGYSFD RLFPDGLFPN DNNQNSRRKA SDARNLLSKM
310 320 330 340 350
LVIDPEQRIS VDEALKHEYI NVWYDAEEVD APAPEPYDHS VDEREHTVEQ
360 370
WKELIYEEVM DYEAHNTNNR TR
Length:372
Mass (Da):43,027
Last modified:May 1, 1997 - v1
Checksum:i96662B278ABCCA19
GO

Sequence cautioni

The sequence AAC47325.1 differs from that shown. Reason: Frameshift at position 331.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti331 – 37242APAPE…NNRTR → RPLRSHMITAWTKGNTLWSS GRS in AAC47325. (PubMed:8946916)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50965 Genomic DNA. Translation: AAB51187.1.
U50966 Genomic DNA. Translation: AAB51188.1.
U49180 mRNA. Translation: AAB97094.1.
U49249 Genomic DNA. Translation: AAB48381.1.
U73196 mRNA. Translation: AAC47325.1. Frameshift.
AE014134 Genomic DNA. Translation: AAF52883.1.
AY122221 mRNA. Translation: AAM52733.1.
AY070865 mRNA. Translation: AAL48487.1.
RefSeqiNP_001162930.1. NM_001169459.1.
NP_001162932.1. NM_001169461.3.
NP_723541.1. NM_164900.3.
UniGeneiDm.1448.

Genome annotation databases

EnsemblMetazoaiFBtr0080087; FBpp0079676; FBgn0000229.
FBtr0300982; FBpp0290204; FBgn0000229.
FBtr0302378; FBpp0291573; FBgn0000229.
GeneIDi44801.
KEGGidme:Dmel_CG5680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50965 Genomic DNA. Translation: AAB51187.1 .
U50966 Genomic DNA. Translation: AAB51188.1 .
U49180 mRNA. Translation: AAB97094.1 .
U49249 Genomic DNA. Translation: AAB48381.1 .
U73196 mRNA. Translation: AAC47325.1 . Frameshift.
AE014134 Genomic DNA. Translation: AAF52883.1 .
AY122221 mRNA. Translation: AAM52733.1 .
AY070865 mRNA. Translation: AAL48487.1 .
RefSeqi NP_001162930.1. NM_001169459.1.
NP_001162932.1. NM_001169461.3.
NP_723541.1. NM_164900.3.
UniGenei Dm.1448.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4M3A X-ray 1.96 A 1-372 [» ]
ProteinModelPortali P92208.
SMRi P92208. Positions 9-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 69288. 49 interactions.
DIPi DIP-17307N.
IntActi P92208. 18 interactions.
MINTi MINT-337077.

Proteomic databases

PaxDbi P92208.
PRIDEi P92208.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0080087 ; FBpp0079676 ; FBgn0000229 .
FBtr0300982 ; FBpp0290204 ; FBgn0000229 .
FBtr0302378 ; FBpp0291573 ; FBgn0000229 .
GeneIDi 44801.
KEGGi dme:Dmel_CG5680.

Organism-specific databases

CTDi 44801.
FlyBasei FBgn0000229. bsk.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074271.
InParanoidi P92208.
KOi K04440.
OMAi EVMNFEE.
OrthoDBi EOG7PCJGV.
PhylomeDBi P92208.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 1994.
Reactomei REACT_181694. JNK (c-Jun kinases) phosphorylation and activation mediated by activated human TAK1.
REACT_181712. Oxidative Stress Induced Senescence.
REACT_202759. FCERI mediated MAPK activation.
REACT_34513. DSCAM interactions.
SignaLinki P92208.

Miscellaneous databases

GenomeRNAii 44801.
NextBioi 837648.

Gene expression databases

Bgeei P92208.
ExpressionAtlasi P92208. differential.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008351. MAPK_JNK.
IPR000719. Prot_kinase_dom.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01772. JNKMAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A JNK signal transduction pathway that mediates morphogenesis and an immune response in Drosophila."
    Sluss H.K., Han Z., Barrett T., Davis R.J., Ip Y.T.
    Genes Dev. 10:2745-2758(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. "The Drosophila Jun-N-terminal kinase is required for cell morphogenesis but not for DJun-dependent cell fate specification in the eye."
    Riesgo-Escovar J.R., Jenni M., Fritz A., Hafen E.
    Genes Dev. 10:2759-2768(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS, TISSUE SPECIFICITY.
    Strain: Oregon-R.
    Tissue: Embryo.
  3. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  4. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo and Head.
  6. "Drosophila Jun kinase regulates expression of decapentaplegic via the ETS-domain protein Aop and the AP-1 transcription factor DJun during dorsal closure."
    Riesgo-Escovar J.R., Hafen E.
    Genes Dev. 11:1717-1727(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Thorax closure in Drosophila: involvement of Fos and the JNK pathway."
    Zeitlinger J., Bohmann D.
    Development 126:3947-3956(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION.
  8. "JNK signaling pathway is required for efficient wound healing in Drosophila."
    Raemet M., Lanot R., Zachary D., Manfruelli P.
    Dev. Biol. 241:145-156(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiJNK_DROME
AccessioniPrimary (citable) accession number: P92208
Secondary accession number(s): O01366, Q94542
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3