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Protein

Negative elongation factor E

Gene

Nelf-E

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex, causes transcriptional pausing.1 Publication

GO - Molecular functioni

  • mRNA binding Source: FlyBase
  • RNA binding Source: UniProtKB

GO - Biological processi

  • negative regulation of DNA-templated transcription, elongation Source: UniProtKB
  • negative regulation of transcription elongation from RNA polymerase II promoter Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter during mitotic cell cycle Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • transcription elongation from RNA polymerase II promoter Source: UniProtKB

Keywordsi

Molecular functionRepressor, RNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-112382. Formation of RNA Pol II elongation complex.
R-DME-113418. Formation of the Early Elongation Complex.
R-DME-674695. RNA Polymerase II Pre-transcription Events.
R-DME-6796648. TP53 Regulates Transcription of DNA Repair Genes.
R-DME-75955. RNA Polymerase II Transcription Elongation.
SignaLinkiP92204.

Names & Taxonomyi

Protein namesi
Recommended name:
Negative elongation factor E
Gene namesi
Name:Nelf-E
Synonyms:anon-66Da
ORF Names:CG5994
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0017430. Nelf-E.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000818041 – 280Negative elongation factor EAdd BLAST280

Proteomic databases

PaxDbiP92204.
PRIDEiP92204.

Expressioni

Gene expression databases

BgeeiFBgn0017430.
GenevisibleiP92204. DM.

Interactioni

Subunit structurei

Component of the NELF complex, which is at least composed of TH1/Nelf-D and Nelf-E.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Hsp83P028282EBI-194490,EBI-136814

Protein-protein interaction databases

BioGridi64390. 19 interactors.
IntActiP92204. 5 interactors.
MINTiMINT-779659.
STRINGi7227.FBpp0076374.

Structurei

3D structure databases

ProteinModelPortaliP92204.
SMRiP92204.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 236RRMPROSITE-ProRule annotationAdd BLAST71

Sequence similaritiesi

Belongs to the RRM NELF-E family.Curated

Phylogenomic databases

eggNOGiENOG410IK4K. Eukaryota.
ENOG41101UX. LUCA.
GeneTreeiENSGT00630000089917.
InParanoidiP92204.
KOiK15182.
OMAiSEMDGSM.
OrthoDBiEOG091G0I1Z.
PhylomeDBiP92204.

Family and domain databases

CDDicd12305. RRM_NELFE. 1 hit.
InterProiView protein in InterPro
IPR033102. NELFE.
IPR034637. NELFE_RRM.
IPR035979. RBD_domain_sf.
IPR000504. RRM_dom.
PANTHERiPTHR17250. PTHR17250. 2 hits.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

P92204-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVYIHFPNNL TEEEQMLQAK YQKLKKKKKA LQAHKAPKPE PESSLTLKRP
60 70 80 90 100
TDARDAREVA RKLIKSGAIP AIQKQTKQDQ TSFKRPKGQE RAKRSTSETT
110 120 130 140 150
VASYQPFSST QNDVAQETII SEIIKEEPRR QNLYQHFATE RDREERGMPE
160 170 180 190 200
KVPMDTAQPE KPRAGNTIFV SGNKVTEDFL KKTFNDYGTI VNVSMEIEKS
210 220 230 240 250
RGFVSFAKPE SADRAIAEIH GKNVNGINLQ VQLARRQPQI EPINDASSSA
260 270 280
VWSSIAASKS QKGSHKDHRE MVQYDEDFLL
Length:280
Mass (Da):31,811
Last modified:May 1, 1997 - v1
Checksum:i9F1F351727D53C47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y10015 Genomic DNA. Translation: CAA71120.1.
Y10016 mRNA. Translation: CAA71123.1.
AE014296 Genomic DNA. Translation: AAF50394.1.
AY071061 mRNA. Translation: AAL48683.1.
RefSeqiNP_648241.1. NM_139984.3.
UniGeneiDm.20046.

Genome annotation databases

EnsemblMetazoaiFBtr0076650; FBpp0076374; FBgn0017430.
GeneIDi38982.
KEGGidme:Dmel_CG5994.

Similar proteinsi

Entry informationi

Entry nameiNELFE_DROME
AccessioniPrimary (citable) accession number: P92204
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 14, 2003
Last sequence update: May 1, 1997
Last modified: October 25, 2017
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families