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Protein

Larval cuticle protein 8

Gene

Lcp65Ag1

more
Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the cuticle of the larva.

GO - Molecular functioni

GO - Biological processi

  • chitin-based cuticle development Source: FlyBase
  • larval chitin-based cuticle development Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Larval cuticle protein 8
Alternative name(s):
Larval cuticle protein VIII
Gene namesi
Name:Lcp65Ag1
Synonyms:DCP8-alpha, Lcp8
ORF Names:CG10530
AND
Name:Lcp65Ag2
Synonyms:DCP8-beta, Lcp8
ORF Names:CG10534
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0020638. Lcp65Ag1.
FBgn0020637. Lcp65Ag2.

Subcellular locationi

GO - Cellular componenti

  • extracellular matrix Source: FlyBase
  • extracellular region Source: UniProtKB
  • extracellular space Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cuticle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 18181 PublicationAdd
BLAST
Chaini19 – 10587Larval cuticle protein 8PRO_0000006395Add
BLAST

Proteomic databases

PaxDbiP92201.
PRIDEiP92201.

Expressioni

Developmental stagei

Expression begins in late embryo and end late third larval instar. Maximal expression is at the end of the first larval instar.1 Publication

Gene expression databases

BgeeiP92201.
ExpressionAtlasiP92201. differential.
GenevisibleiP92201. DM.

Interactioni

Protein-protein interaction databases

BioGridi64157. 7 interactions.
64158. 7 interactions.
DIPiDIP-18387N.
IntActiP92201. 6 interactions.
MINTiMINT-813739.
STRINGi7227.FBpp0076767.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 10370Chitin-binding type R&RPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type R&R domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00530000064111.
InParanoidiP92201.
OMAiTDNEYIA.
OrthoDBiEOG72JWJ7.
PhylomeDBiP92201.

Family and domain databases

InterProiIPR031311. CHIT_BIND_RR_consensus.
IPR000618. Insect_cuticle.
[Graphical view]
PfamiPF00379. Chitin_bind_4. 1 hit.
[Graphical view]
PRINTSiPR00947. CUTICLE.
PROSITEiPS00233. CHIT_BIND_RR_1. 1 hit.
PS51155. CHIT_BIND_RR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P92201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFLIVFVAL FAVALAAPAA EEPTIVRSES DVGPESFKYD WETSDGQAAQ
60 70 80 90 100
AVGQLNDIGT ENEAISVSGS YRFIADDGQT YQVNYIADKN GFQPQGAHLP

VAPVA
Length:105
Mass (Da):11,194
Last modified:May 1, 1997 - v1
Checksum:iF23DA9A5BAB21D2F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50692.1.
AE014296 Genomic DNA. Translation: AAF50693.1.
U84753 Genomic DNA. Translation: AAB88071.1.
U84754 Genomic DNA. Translation: AAB88072.1.
AY061536 mRNA. Translation: AAL29084.1.
RefSeqiNP_477272.1. NM_057924.3.
NP_477273.1. NM_057925.3.
UniGeneiDm.20036.
Dm.6527.

Genome annotation databases

EnsemblMetazoaiFBtr0077058; FBpp0076766; FBgn0020638.
FBtr0077059; FBpp0076767; FBgn0020637.
GeneIDi38702.
38703.
KEGGidme:Dmel_CG10530.
dme:Dmel_CG10534.
UCSCiCG10530-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014296 Genomic DNA. Translation: AAF50692.1.
AE014296 Genomic DNA. Translation: AAF50693.1.
U84753 Genomic DNA. Translation: AAB88071.1.
U84754 Genomic DNA. Translation: AAB88072.1.
AY061536 mRNA. Translation: AAL29084.1.
RefSeqiNP_477272.1. NM_057924.3.
NP_477273.1. NM_057925.3.
UniGeneiDm.20036.
Dm.6527.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi64157. 7 interactions.
64158. 7 interactions.
DIPiDIP-18387N.
IntActiP92201. 6 interactions.
MINTiMINT-813739.
STRINGi7227.FBpp0076767.

Proteomic databases

PaxDbiP92201.
PRIDEiP92201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0077058; FBpp0076766; FBgn0020638.
FBtr0077059; FBpp0076767; FBgn0020637.
GeneIDi38702.
38703.
KEGGidme:Dmel_CG10530.
dme:Dmel_CG10534.
UCSCiCG10530-RA. d. melanogaster.

Organism-specific databases

CTDi38702.
38703.
FlyBaseiFBgn0020638. Lcp65Ag1.
FBgn0020637. Lcp65Ag2.

Phylogenomic databases

GeneTreeiENSGT00530000064111.
InParanoidiP92201.
OMAiTDNEYIA.
OrthoDBiEOG72JWJ7.
PhylomeDBiP92201.

Miscellaneous databases

ChiTaRSiLcp65Ag2. fly.
PROiP92201.

Gene expression databases

BgeeiP92201.
ExpressionAtlasiP92201. differential.
GenevisibleiP92201. DM.

Family and domain databases

InterProiIPR031311. CHIT_BIND_RR_consensus.
IPR000618. Insect_cuticle.
[Graphical view]
PfamiPF00379. Chitin_bind_4. 1 hit.
[Graphical view]
PRINTSiPR00947. CUTICLE.
PROSITEiPS00233. CHIT_BIND_RR_1. 1 hit.
PS51155. CHIT_BIND_RR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A cluster of cuticle genes of Drosophila at 65A: sequence, structure and evolution."
    Charles J.-P., Chihara C., Nejad S., Riddiford L.M.
    Genetics 147:1213-1224(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (LCP65AG1 AND LCP65AG2).
    Strain: Iso-1.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] (LCP65AG1 AND LCP65AG2).
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (LCP65AG1).
    Strain: Berkeley.
  5. "Identification of proteins and developmental expression of RNAs encoded by the 65A cuticle protein gene cluster in Drosophila melanogaster."
    Charles J.-P., Chihara C., Nejad S., Riddiford L.M.
    Insect Biochem. Mol. Biol. 28:131-138(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 19-28, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
    Tissue: Larva.

Entry informationi

Entry nameiLCP8_DROME
AccessioniPrimary (citable) accession number: P92201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 10, 2003
Last sequence update: May 1, 1997
Last modified: July 6, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.