Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P92177

- 1433E_DROME

UniProt

P92177 - 1433E_DROME

Protein

14-3-3 protein epsilon

Gene

14-3-3epsilon

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 2 (19 Sep 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization.1 Publication

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein heterodimerization activity Source: FlyBase

    GO - Biological processi

    1. axon guidance Source: FlyBase
    2. determination of adult lifespan Source: FlyBase
    3. DNA damage checkpoint Source: FlyBase
    4. germarium-derived oocyte fate determination Source: FlyBase
    5. imaginal disc development Source: FlyBase
    6. mitotic cell cycle checkpoint Source: FlyBase
    7. mitotic G2 DNA damage checkpoint Source: FlyBase
    8. oocyte microtubule cytoskeleton polarization Source: FlyBase
    9. pole cell migration Source: FlyBase
    10. positive regulation of growth Source: FlyBase
    11. regulation of growth Source: FlyBase
    12. regulation of mitosis Source: FlyBase
    13. response to radiation Source: FlyBase
    14. response to UV Source: FlyBase
    15. wing disc dorsal/ventral pattern formation Source: FlyBase

    Enzyme and pathway databases

    ReactomeiREACT_210254. Regulation of HSF1-mediated heat shock response.
    REACT_227922. HSF1 activation.
    SignaLinkiP92177.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    14-3-3 protein epsilon
    Alternative name(s):
    Suppressor of Ras1 3-9
    Gene namesi
    Name:14-3-3epsilon
    Synonyms:14-3-3e, SR3-9
    ORF Names:CG31196
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0020238. 14-3-3epsilon.

    Subcellular locationi

    GO - Cellular componenti

    1. chromosome Source: FlyBase
    2. cytoplasm Source: FlyBase
    3. germline ring canal Source: FlyBase
    4. microtubule associated complex Source: FlyBase
    5. nucleus Source: FlyBase

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi183 – 1831E → K: Suppressor of sev-Ras1 V12; subviable. 1 Publication
    Mutagenesisi199 – 1991F → Y: Suppressor of sev-Ras1 V12. 1 Publication
    Mutagenesisi214 – 2141Y → F: Suppressor of sev-Ras1 V12. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 26226214-3-3 protein epsilonPRO_0000058650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei262 – 2621Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP92177.
    PRIDEiP92177.

    Expressioni

    Gene expression databases

    BgeeiP92177.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Interacts with phosphorylated yki.By similarity2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    bazQ9VX755EBI-204478,EBI-2295779

    Protein-protein interaction databases

    BioGridi67207. 28 interactions.
    DIPiDIP-18498N.
    IntActiP92177. 234 interactions.
    MINTiMINT-277717.
    STRINGi7227.FBpp0082987.

    Structurei

    3D structure databases

    ProteinModelPortaliP92177.
    SMRiP92177. Positions 3-232.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 14-3-3 family.Curated

    Phylogenomic databases

    eggNOGiCOG5040.
    GeneTreeiENSGT00730000110613.
    InParanoidiP92177.
    KOiK06630.
    OMAiMQESDKP.
    OrthoDBiEOG7HHWT3.
    PhylomeDBiP92177.

    Family and domain databases

    Gene3Di1.20.190.20. 1 hit.
    InterProiIPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view]
    PANTHERiPTHR18860. PTHR18860. 1 hit.
    PfamiPF00244. 14-3-3. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000868. 14-3-3. 1 hit.
    PRINTSiPR00305. 1433ZETA.
    SMARTiSM00101. 14_3_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF48445. SSF48445. 1 hit.
    PROSITEiPS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms may exist.

    Isoform A (identifier: P92177-3) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK    50
    NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI 100
    LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI 150
    AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD 200
    DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV DPNAGDGEPK 250
    EQIQDVEDQD VS 262

    Note: No experimental confirmation available.

    Length:262
    Mass (Da):29,799
    Last modified:September 19, 2003 - v2
    Checksum:i2C1562208547DA9C
    GO
    Isoform B (identifier: P92177-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         239-239: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:261
    Mass (Da):29,669
    Checksum:i8195754CE0B089AD
    GO
    Isoform C (identifier: P92177-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         239-244: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:256
    Mass (Da):29,173
    Checksum:i0B2FEE0EA6D36219
    GO
    Isoform D (identifier: P92177-1) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         239-240: Missing.

    Show »
    Length:260
    Mass (Da):29,570
    Checksum:i1282192917E3A7A4
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei239 – 2446Missing in isoform C. CuratedVSP_026086
    Alternative sequencei239 – 2402Missing in isoform D. 1 PublicationVSP_008204
    Alternative sequencei239 – 2391Missing in isoform B. CuratedVSP_008203

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U84898 Genomic DNA. Translation: AAC47520.1.
    U84897 mRNA. Translation: AAC47519.1.
    AE014297 Genomic DNA. Translation: AAF55519.2.
    AE014297 Genomic DNA. Translation: AAN13764.1.
    AE014297 Genomic DNA. Translation: AAN13765.1.
    AE014297 Genomic DNA. Translation: AAN13766.1.
    RefSeqiNP_732309.1. NM_169796.2. [P92177-3]
    NP_732310.1. NM_169797.2. [P92177-2]
    NP_732311.1. NM_169798.2. [P92177-1]
    NP_732312.1. NM_169799.2. [P92177-4]
    UniGeneiDm.2357.

    Genome annotation databases

    EnsemblMetazoaiFBtr0083565; FBpp0082987; FBgn0020238. [P92177-3]
    GeneIDi42186.
    KEGGidme:Dmel_CG31196.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U84898 Genomic DNA. Translation: AAC47520.1 .
    U84897 mRNA. Translation: AAC47519.1 .
    AE014297 Genomic DNA. Translation: AAF55519.2 .
    AE014297 Genomic DNA. Translation: AAN13764.1 .
    AE014297 Genomic DNA. Translation: AAN13765.1 .
    AE014297 Genomic DNA. Translation: AAN13766.1 .
    RefSeqi NP_732309.1. NM_169796.2. [P92177-3 ]
    NP_732310.1. NM_169797.2. [P92177-2 ]
    NP_732311.1. NM_169798.2. [P92177-1 ]
    NP_732312.1. NM_169799.2. [P92177-4 ]
    UniGenei Dm.2357.

    3D structure databases

    ProteinModelPortali P92177.
    SMRi P92177. Positions 3-232.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 67207. 28 interactions.
    DIPi DIP-18498N.
    IntActi P92177. 234 interactions.
    MINTi MINT-277717.
    STRINGi 7227.FBpp0082987.

    Proteomic databases

    PaxDbi P92177.
    PRIDEi P92177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0083565 ; FBpp0082987 ; FBgn0020238 . [P92177-3 ]
    GeneIDi 42186.
    KEGGi dme:Dmel_CG31196.

    Organism-specific databases

    CTDi 42186.
    FlyBasei FBgn0020238. 14-3-3epsilon.

    Phylogenomic databases

    eggNOGi COG5040.
    GeneTreei ENSGT00730000110613.
    InParanoidi P92177.
    KOi K06630.
    OMAi MQESDKP.
    OrthoDBi EOG7HHWT3.
    PhylomeDBi P92177.

    Enzyme and pathway databases

    Reactomei REACT_210254. Regulation of HSF1-mediated heat shock response.
    REACT_227922. HSF1 activation.
    SignaLinki P92177.

    Miscellaneous databases

    ChiTaRSi 14-3-3epsilon. drosophila.
    GenomeRNAii 42186.
    NextBioi 827573.
    PROi P92177.

    Gene expression databases

    Bgeei P92177.

    Family and domain databases

    Gene3Di 1.20.190.20. 1 hit.
    InterProi IPR000308. 14-3-3.
    IPR023409. 14-3-3_CS.
    IPR023410. 14-3-3_domain.
    [Graphical view ]
    PANTHERi PTHR18860. PTHR18860. 1 hit.
    Pfami PF00244. 14-3-3. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000868. 14-3-3. 1 hit.
    PRINTSi PR00305. 1433ZETA.
    SMARTi SM00101. 14_3_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48445. SSF48445. 1 hit.
    PROSITEi PS00796. 1433_1. 1 hit.
    PS00797. 1433_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "14-3-3 epsilon positively regulates Ras-mediated signaling in Drosophila."
      Chang H.C., Rubin G.M.
      Genes Dev. 11:1132-1139(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM D), MUTAGENESIS OF GLU-183; PHE-199 AND TYR-214.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.
    4. "An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
      Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
      Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, IDENTIFICATION BY MASS SPECTROMETRY.
    5. "In vivo regulation of Yorkie phosphorylation and localization."
      Oh H., Irvine K.D.
      Development 135:1081-1088(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH YKI.
    6. "Hippo signaling regulates Yorkie nuclear localization and activity through 14-3-3 dependent and independent mechanisms."
      Ren F., Zhang L., Jiang J.
      Dev. Biol. 337:303-312(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH YKI.

    Entry informationi

    Entry namei1433E_DROME
    AccessioniPrimary (citable) accession number: P92177
    Secondary accession number(s): Q8IN86, Q8IN87, Q9VEA8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 19, 2003
    Last modified: October 1, 2014
    This is version 121 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3