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P92177 (1433E_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
14-3-3 protein epsilon
Alternative name(s):
Suppressor of Ras1 3-9
Gene names
Name:14-3-3epsilon
Synonyms:14-3-3e, SR3-9
ORF Names:CG31196
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization. Ref.6

Subunit structure

Homodimer By similarity. Interacts with phosphorylated yki. Ref.5 Ref.6

Sequence similarities

Belongs to the 14-3-3 family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA damage checkpoint

Inferred from mutant phenotype PubMed 10733527PubMed 11682604. Source: FlyBase

axon guidance

Inferred from mutant phenotype PubMed 22500634. Source: FlyBase

determination of adult lifespan

Inferred from mutant phenotype PubMed 18665908. Source: FlyBase

germarium-derived oocyte fate determination

Inferred from mutant phenotype PubMed 12431373. Source: FlyBase

imaginal disc development

Traceable author statement PubMed 11063934. Source: FlyBase

mitotic G2 DNA damage checkpoint

Inferred from genetic interaction PubMed 21205937. Source: FlyBase

mitotic cell cycle checkpoint

Traceable author statement PubMed 11063934. Source: FlyBase

oocyte microtubule cytoskeleton polarization

Inferred from mutant phenotype PubMed 12431373. Source: FlyBase

pole cell migration

Inferred from mutant phenotype PubMed 22666326. Source: FlyBase

positive regulation of growth

Inferred from mutant phenotype PubMed 18665908. Source: FlyBase

regulation of growth

Inferred from genetic interaction PubMed 18665908. Source: FlyBase

regulation of mitosis

Inferred from mutant phenotype PubMed 11682604. Source: FlyBase

response to UV

Inferred from mutant phenotype PubMed 18665908. Source: FlyBase

response to radiation

Traceable author statement PubMed 11063934. Source: FlyBase

wing disc dorsal/ventral pattern formation

Inferred from genetic interaction PubMed 18202376. Source: FlyBase

   Cellular_componentchromosome

Inferred from direct assay PubMed 11682604. Source: FlyBase

cytoplasm

Inferred from direct assay PubMed 11682604. Source: FlyBase

germline ring canal

Inferred from direct assay PubMed 12431373. Source: FlyBase

microtubule associated complex

Inferred from direct assay PubMed 18433294. Source: FlyBase

nucleus

Inferred from direct assay PubMed 11682604. Source: FlyBase

   Molecular_functionprotein heterodimerization activity

Inferred from physical interaction PubMed 19920133. Source: FlyBase

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

bazQ9VX755EBI-204478,EBI-2295779

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms may exist.
Isoform A (identifier: P92177-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Note: No experimental confirmation available.
Isoform B (identifier: P92177-2)

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: Missing.
Note: No experimental confirmation available.
Isoform C (identifier: P92177-4)

The sequence of this isoform differs from the canonical sequence as follows:
     239-244: Missing.
Note: No experimental confirmation available.
Isoform D (identifier: P92177-1)

The sequence of this isoform differs from the canonical sequence as follows:
     239-240: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 26226214-3-3 protein epsilon
PRO_0000058650

Amino acid modifications

Modified residue2621Phosphoserine Ref.4

Natural variations

Alternative sequence239 – 2446Missing in isoform C.
VSP_026086
Alternative sequence239 – 2402Missing in isoform D.
VSP_008204
Alternative sequence2391Missing in isoform B.
VSP_008203

Experimental info

Mutagenesis1831E → K: Suppressor of sev-Ras1 V12; subviable. Ref.1
Mutagenesis1991F → Y: Suppressor of sev-Ras1 V12. Ref.1
Mutagenesis2141Y → F: Suppressor of sev-Ras1 V12. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified September 19, 2003. Version 2.
Checksum: 2C1562208547DA9C

FASTA26229,799
        10         20         30         40         50         60 
MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW 

        70         80         90        100        110        120 
RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF 

       130        140        150        160        170        180 
YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF 

       190        200        210        220        230        240 
YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV 

       250        260 
DPNAGDGEPK EQIQDVEDQD VS 

« Hide

Isoform B [UniParc].

Checksum: 8195754CE0B089AD
Show »

FASTA26129,669
Isoform C [UniParc].

Checksum: 0B2FEE0EA6D36219
Show »

FASTA25629,173
Isoform D [UniParc].

Checksum: 1282192917E3A7A4
Show »

FASTA26029,570

References

« Hide 'large scale' references
[1]"14-3-3 epsilon positively regulates Ras-mediated signaling in Drosophila."
Chang H.C., Rubin G.M.
Genes Dev. 11:1132-1139(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM D), MUTAGENESIS OF GLU-183; PHE-199 AND TYR-214.
[2]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[3]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
Strain: Berkeley.
[4]"An integrated chemical, mass spectrometric and computational strategy for (quantitative) phosphoproteomics: application to Drosophila melanogaster Kc167 cells."
Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A., Eng J.K., Aebersold R., Tao W.A.
Mol. Biosyst. 3:275-286(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-262, IDENTIFICATION BY MASS SPECTROMETRY.
[5]"In vivo regulation of Yorkie phosphorylation and localization."
Oh H., Irvine K.D.
Development 135:1081-1088(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH YKI.
[6]"Hippo signaling regulates Yorkie nuclear localization and activity through 14-3-3 dependent and independent mechanisms."
Ren F., Zhang L., Jiang J.
Dev. Biol. 337:303-312(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH YKI.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U84898 Genomic DNA. Translation: AAC47520.1.
U84897 mRNA. Translation: AAC47519.1.
AE014297 Genomic DNA. Translation: AAF55519.2.
AE014297 Genomic DNA. Translation: AAN13764.1.
AE014297 Genomic DNA. Translation: AAN13765.1.
AE014297 Genomic DNA. Translation: AAN13766.1.
RefSeqNP_732309.1. NM_169796.2.
NP_732310.1. NM_169797.2.
NP_732311.1. NM_169798.2.
NP_732312.1. NM_169799.2.
UniGeneDm.2357.

3D structure databases

ProteinModelPortalP92177.
SMRP92177. Positions 3-232.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid67207. 27 interactions.
DIPDIP-18498N.
IntActP92177. 234 interactions.
MINTMINT-277717.
STRING7227.FBpp0082987.

Proteomic databases

PaxDbP92177.
PRIDEP92177.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0083565; FBpp0082987; FBgn0020238. [P92177-3]
GeneID42186.
KEGGdme:Dmel_CG31196.

Organism-specific databases

CTD42186.
FlyBaseFBgn0020238. 14-3-3epsilon.

Phylogenomic databases

eggNOGCOG5040.
GeneTreeENSGT00730000110613.
InParanoidP92177.
KOK06630.
OMAMQESDKP.
OrthoDBEOG7HHWT3.
PhylomeDBP92177.

Enzyme and pathway databases

SignaLinkP92177.

Gene expression databases

BgeeP92177.

Family and domain databases

Gene3D1.20.190.20. 1 hit.
InterProIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERPTHR18860. PTHR18860. 1 hit.
PfamPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFPIRSF000868. 14-3-3. 1 hit.
PRINTSPR00305. 1433ZETA.
SMARTSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMSSF48445. SSF48445. 1 hit.
PROSITEPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRS14-3-3epsilon. drosophila.
GenomeRNAi42186.
NextBio827573.
PROP92177.

Entry information

Entry name1433E_DROME
AccessionPrimary (citable) accession number: P92177
Secondary accession number(s): Q8IN86, Q8IN87, Q9VEA8
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 19, 2003
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase