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Protein

14-3-3 protein epsilon

Gene

14-3-3epsilon

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization.1 Publication

GO - Molecular functioni

  • phosphoserine residue binding Source: FlyBase
  • protein domain specific binding Source: InterPro
  • protein heterodimerization activity Source: FlyBase

GO - Biological processi

  • axon guidance Source: FlyBase
  • determination of adult lifespan Source: FlyBase
  • DNA damage checkpoint Source: FlyBase
  • germarium-derived oocyte fate determination Source: FlyBase
  • imaginal disc development Source: FlyBase
  • mitotic cell cycle checkpoint Source: FlyBase
  • oocyte microtubule cytoskeleton polarization Source: FlyBase
  • pole cell migration Source: FlyBase
  • positive regulation of growth Source: FlyBase
  • positive regulation of Ras protein signal transduction Source: FlyBase
  • regulation of growth Source: FlyBase
  • regulation of mitotic nuclear division Source: FlyBase
  • response to radiation Source: FlyBase
  • response to UV Source: FlyBase
  • wing disc dorsal/ventral pattern formation Source: FlyBase

Enzyme and pathway databases

ReactomeiR-DME-1445148 Translocation of GLUT4 to the plasma membrane
R-DME-2028269 Signaling by Hippo
R-DME-3371453 Regulation of HSF1-mediated heat shock response
R-DME-3371511 HSF1 activation
R-DME-390098 Phosphorylation-dependent inhibition of YKI
R-DME-432553 Phosphorylation of PER and TIM
R-DME-5625740 RHO GTPases activate PKNs
R-DME-5628897 TP53 Regulates Metabolic Genes
R-DME-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex
R-DME-8876198 RAB GEFs exchange GTP for GDP on RABs
SignaLinkiP92177

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein epsilon
Alternative name(s):
Suppressor of Ras1 3-9
Gene namesi
Name:14-3-3epsilon
Synonyms:14-3-3e, SR3-9
ORF Names:CG31196
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0020238 14-3-3epsilon

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi183E → K: Suppressor of sev-Ras1 V12; subviable. 1 Publication1
Mutagenesisi199F → Y: Suppressor of sev-Ras1 V12. 1 Publication1
Mutagenesisi214Y → F: Suppressor of sev-Ras1 V12. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000586501 – 26214-3-3 protein epsilonAdd BLAST262

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei262Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP92177
PRIDEiP92177

PTM databases

iPTMnetiP92177

Expressioni

Gene expression databases

BgeeiFBgn0020238
ExpressionAtlasiP92177 baseline and differential
GenevisibleiP92177 DM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with phosphorylated yki.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
bazQ9VX755EBI-204478,EBI-2295779

GO - Molecular functioni

  • phosphoserine residue binding Source: FlyBase
  • protein domain specific binding Source: InterPro
  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi67207, 54 interactors
DIPiDIP-18498N
IntActiP92177, 235 interactors
MINTiP92177
STRINGi7227.FBpp0082987

Structurei

3D structure databases

ProteinModelPortaliP92177
SMRiP92177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
InParanoidiP92177
KOiK06630
OMAiIPCATTG
OrthoDBiEOG091G0VKY
PhylomeDBiP92177

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms may exist.
Isoform A (identifier: P92177-3) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK
60 70 80 90 100
NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI
110 120 130 140 150
LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI
160 170 180 190 200
AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD
210 220 230 240 250
DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV DPNAGDGEPK
260
EQIQDVEDQD VS
Note: No experimental confirmation available.
Length:262
Mass (Da):29,799
Last modified:September 19, 2003 - v2
Checksum:i2C1562208547DA9C
GO
Isoform B (identifier: P92177-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-239: Missing.

Note: No experimental confirmation available.
Show »
Length:261
Mass (Da):29,669
Checksum:i8195754CE0B089AD
GO
Isoform C (identifier: P92177-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-244: Missing.

Note: No experimental confirmation available.
Show »
Length:256
Mass (Da):29,173
Checksum:i0B2FEE0EA6D36219
GO
Isoform D (identifier: P92177-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     239-240: Missing.

Show »
Length:260
Mass (Da):29,570
Checksum:i1282192917E3A7A4
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_026086239 – 244Missing in isoform C. Curated6
Alternative sequenceiVSP_008204239 – 240Missing in isoform D. 1 Publication2
Alternative sequenceiVSP_008203239Missing in isoform B. Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84898 Genomic DNA Translation: AAC47520.1
U84897 mRNA Translation: AAC47519.1
AE014297 Genomic DNA Translation: AAF55519.2
AE014297 Genomic DNA Translation: AAN13764.1
AE014297 Genomic DNA Translation: AAN13765.1
AE014297 Genomic DNA Translation: AAN13766.1
RefSeqiNP_732309.1, NM_169796.2 [P92177-3]
NP_732310.1, NM_169797.3 [P92177-2]
NP_732311.1, NM_169798.3 [P92177-1]
NP_732312.1, NM_169799.2 [P92177-4]
UniGeneiDm.2357

Genome annotation databases

EnsemblMetazoaiFBtr0083565; FBpp0082987; FBgn0020238 [P92177-3]
FBtr0083566; FBpp0082988; FBgn0020238 [P92177-2]
FBtr0083567; FBpp0082989; FBgn0020238 [P92177-4]
FBtr0083568; FBpp0082990; FBgn0020238 [P92177-1]
GeneIDi42186
KEGGidme:Dmel_CG31196

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry namei1433E_DROME
AccessioniPrimary (citable) accession number: P92177
Secondary accession number(s): Q8IN86, Q8IN87, Q9VEA8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 19, 2003
Last modified: May 23, 2018
This is version 158 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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