P91943 (PANG1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein pangolin, isoforms A/H/I

Alternative name(s):
dTCF

Gene names

Name:	pan
Synonyms:	TCF
ORF Names:	CG34403

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	751 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Segment polarity protein. Functions together with arm to transduce the Wingless (Wg) signal in embryos and in developing adult tissues. Acts as a transcriptional activator, but in the absence of arm, it binds to gro and acts as a transcriptional repressor of wg-responsive genes. Ref.1 Ref.2 Ref.7
Subunit structure	Binds to the beta-catenin homolog arm or to gro. Ref.7
Subcellular location	Nucleus Ref.7.
Disruption phenotype	Flies exhibit a segment polarity phenotype and altered expression of the wg target genes en and Ubx. Ref.1
Sequence similarities	Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation Wnt signaling pathway
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator Developmental protein Repressor Segmentation polarity protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	canonical Wnt receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome embryonic pattern specification Inferred from mutant phenotype PubMed 12730381. Source: FlyBase heart development Inferred from mutant phenotype PubMed 22398840. Source: FlyBase imaginal disc-derived wing morphogenesis Inferred from mutant phenotype PubMed 12730381. Source: FlyBase mesodermal cell fate determination Traceable author statement PubMed 12027431. Source: FlyBase negative regulation of Wnt receptor signaling pathway Non-traceable author statement PubMed 12147138. Source: FlyBase negative regulation of transcription, DNA-dependent Inferred from mutant phenotype Ref.1. Source: UniProtKB positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 15043810. Source: FlyBase regulation of hemocyte differentiation Inferred from mutant phenotype PubMed 15381778. Source: FlyBase regulation of stem cell proliferation Inferred from mutant phenotype PubMed 18633350. Source: FlyBase salivary gland morphogenesis Inferred from mutant phenotype PubMed 17507403. Source: FlyBase segment polarity determination Inferred from electronic annotation. Source: UniProtKB-KW spiracle morphogenesis, open tracheal system Inferred from mutant phenotype PubMed 15930099. Source: FlyBase
Cellular_component	microtubule associated complex Inferred from direct assay PubMed 18433294. Source: FlyBase transcription factor complex Inferred from physical interaction PubMed 20439429. Source: FlyBase
Molecular_function	RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 12482712. Source: FlyBase beta-catenin binding Inferred from Biological aspect of Ancestor. Source: RefGenome sequence-specific DNA binding Inferred from direct assay PubMed 11076769. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
arm	P18824	2	EBI-147301,EBI-216128
gro	P16371	2	EBI-147301,EBI-153866

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P91943-3)

Also known as: C; D; E; F; G;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform H (identifier: P91943-4)

The sequence of this isoform differs from the canonical sequence as follows:
     313-364: ELSREEQSKY...RKKDRSTTDS → ALGREEQAKY...KRKRKQDTND

Note: No experimental confirmation available.

Isoform I (identifier: P91943-5)

The sequence of this isoform differs from the canonical sequence as follows:
     313-364: ELSREEQSKY...RKKDRSTTDS → ALGREEQAKY...KRKRKQDTND
     390-414: RKKKCIRYMEALNGNGPAEDGSCFD → YVLPLFLFTMLYILLQMDLQDSRKN
     415-751: Missing.

Note: No experimental confirmation available.

Isoform J (identifier: Q8IMA8-1)

The sequence of this isoform can be found in the external entry Q8IMA8.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 751	751	Protein pangolin, isoforms A/H/I		PRO_0000048601
Regions
	DNA binding	273 – 341	69	HMG box
	Region	1 – 53	53	Beta-catenin binding site By similarity
	Motif	351 – 357	7	Nuclear localization signal Potential
	Compositional bias	351 – 357	7	Poly-Lys
Natural variations
	Alternative sequence	313 – 364	52	ELSRE…STTDS → ALGREEQAKYYELARRERQL HMQMYPDWSSRTNASRGKKR KRKQDTND in isoform H and isoform I.		VSP_021967
	Alternative sequence	390 – 414	25	RKKKC…GSCFD → YVLPLFLFTMLYILLQMDLQ DSRKN in isoform I.		VSP_021968
	Alternative sequence	415 – 751	337	Missing in isoform I.		VSP_021969

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (C) (D) (E) (F) (G) [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 1C6E5A0B36017143	FASTA	751	81,892
	10 20 30 40 50 60 MPHTHSRHGS SGDDLCSTDE VKIFKDEGDR EDEKISSENL LVEEKSSLID LTESEEKGHK 70 80 90 100 110 120 ISRPDHSPVF NKLDTHAPSF NMGYLVSPYS YANGSPSGLP VTMANKIGLP PFFCHNADPL 130 140 150 160 170 180 STPPPAHCGI PPYQLDPKMG LTRPALYPFA GGQYPYPMLS SDMSQVASWH TPSVYSASSF 190 200 210 220 230 240 RTPYPSSLPI NTTLASDFPF RFSPSLLPSV HATSHHVINA HSAIVGVSSK QECGVQDPTT 250 260 270 280 290 300 NNRYPRNLEA KHTSNAQSNE SKETTNDKKK PHIKKPLNAF MLYMKEMRAK VVAECTLKES 310 320 330 340 350 360 AAINQILGRR WHELSREEQS KYYEKARQER QLHMELYPGW SARDNYGYVS KKKKRKKDRS 370 380 390 400 410 420 TTDSGGNNMK KCRARFGLDQ QSQWCKPCRR KKKCIRYMEA LNGNGPAEDG SCFDEHGSQL 430 440 450 460 470 480 SDDDEDDYDD DKLGGSCGSA DETNKIEDED SESLNQSMPS PGCLSGLSSL QSPSTTMSLA 490 500 510 520 530 540 SPLNMNANSA TNVIFPASSN ALLIVGADQP TAQQRPTLVS TSGSSSGSTS SISTTPNTSS 550 560 570 580 590 600 TVSPVTCMTG PCLGSSQERA MMLGNRFSHL GMGLSPPVVS TSTSKSEPFF KPHPTVCNNP 610 620 630 640 650 660 IFALPSIGNC SLNISSMPNT SRNPIGANPR DINNPLSINQ LTKRREYKNV ELIEASESKT 670 680 690 700 710 720 IVAHAATSII QHVAVNGYHA NHSLLNSNLG HLHHQLNNRT ENPNRSEQTM LSVSNHSVNS 730 740 750 SECHKESDSQ AIVSSNPPNA GSSDNGVISV S
	Isoform H [UniParc]. Checksum: 227A1983477B38C8	FASTA	747	81,391
	10 20 30 40 50 60 MPHTHSRHGS SGDDLCSTDE VKIFKDEGDR EDEKISSENL LVEEKSSLID LTESEEKGHK 70 80 90 100 110 120 ISRPDHSPVF NKLDTHAPSF NMGYLVSPYS YANGSPSGLP VTMANKIGLP PFFCHNADPL 130 140 150 160 170 180 STPPPAHCGI PPYQLDPKMG LTRPALYPFA GGQYPYPMLS SDMSQVASWH TPSVYSASSF 190 200 210 220 230 240 RTPYPSSLPI NTTLASDFPF RFSPSLLPSV HATSHHVINA HSAIVGVSSK QECGVQDPTT 250 260 270 280 290 300 NNRYPRNLEA KHTSNAQSNE SKETTNDKKK PHIKKPLNAF MLYMKEMRAK VVAECTLKES 310 320 330 340 350 360 AAINQILGRR WHALGREEQA KYYELARRER QLHMQMYPDW SSRTNASRGK KRKRKQDTND 370 380 390 400 410 420 GGNNMKKCRA RFGLDQQSQW CKPCRRKKKC IRYMEALNGN GPAEDGSCFD EHGSQLSDDD 430 440 450 460 470 480 EDDYDDDKLG GSCGSADETN KIEDEDSESL NQSMPSPGCL SGLSSLQSPS TTMSLASPLN 490 500 510 520 530 540 MNANSATNVI FPASSNALLI VGADQPTAQQ RPTLVSTSGS SSGSTSSIST TPNTSSTVSP 550 560 570 580 590 600 VTCMTGPCLG SSQERAMMLG NRFSHLGMGL SPPVVSTSTS KSEPFFKPHP TVCNNPIFAL 610 620 630 640 650 660 PSIGNCSLNI SSMPNTSRNP IGANPRDINN PLSINQLTKR REYKNVELIE ASESKTIVAH 670 680 690 700 710 720 AATSIIQHVA VNGYHANHSL LNSNLGHLHH QLNNRTENPN RSEQTMLSVS NHSVNSSECH 730 740 KESDSQAIVS SNPPNAGSSD NGVISVS
	Isoform I [UniParc]. Checksum: D80032389302FA84	FASTA	410	46,291
	10 20 30 40 50 60 MPHTHSRHGS SGDDLCSTDE VKIFKDEGDR EDEKISSENL LVEEKSSLID LTESEEKGHK 70 80 90 100 110 120 ISRPDHSPVF NKLDTHAPSF NMGYLVSPYS YANGSPSGLP VTMANKIGLP PFFCHNADPL 130 140 150 160 170 180 STPPPAHCGI PPYQLDPKMG LTRPALYPFA GGQYPYPMLS SDMSQVASWH TPSVYSASSF 190 200 210 220 230 240 RTPYPSSLPI NTTLASDFPF RFSPSLLPSV HATSHHVINA HSAIVGVSSK QECGVQDPTT 250 260 270 280 290 300 NNRYPRNLEA KHTSNAQSNE SKETTNDKKK PHIKKPLNAF MLYMKEMRAK VVAECTLKES 310 320 330 340 350 360 AAINQILGRR WHALGREEQA KYYELARRER QLHMQMYPDW SSRTNASRGK KRKRKQDTND 370 380 390 400 410 GGNNMKKCRA RFGLDQQSQW CKPCRYVLPL FLFTMLYILL QMDLQDSRKN
	Isoform J [UniParc]. See Q8IMA8.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF." van de Wetering M., Cavallo R.A., Dooijes D., van Beest M., van Es J., Loureiro J., Ypma A., Hursh D., Jones T., Bejsovec A., Peifer M., Mortin M., Clevers H. Cell 88:789-799(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, DISRUPTION PHENOTYPE.
[2]	"Pangolin encodes a Lef-1 homologue that acts downstream of Armadillo to transduce the Wingless signal in Drosophila." Brunner E., Peter O., Schweizer L., Basler K. Nature 385:829-833(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING. Strain: Berkeley.
[5]	Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Kapadia B., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 363-751 (ISOFORM J). Strain: Berkeley. Tissue: Head.
[6]	"Recombination, dominance and selection on amino acid polymorphism in the Drosophila genome: contrasting patterns on the X and fourth chromosomes." Sheldahl L.A., Weinreich D.M., Rand D.M. Genetics 165:1195-1208(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 390-751. Strain: Crete24, Crete26, Crete30, Crete31, Crete35, Crete40, Crete42, Crete43, Crete44, Crete8, FTF1, FTF105, FTF14, FTF2, FTF20, FTF23, FTF26, FTF28, FTF5, FTF6, Zim11, Zim2, Zim30 and Zim53.
[7]	"Drosophila Tcf and Groucho interact to repress Wingless signalling activity." Cavallo R.A., Cox R.T., Moline M.M., Roose J., Polevoy G.A., Clevers H., Peifer M., Bejsovec A. Nature 395:604-608(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH ARM AND GRO, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09125 mRNA. Translation: CAA70343.1. U88538 mRNA. Translation: AAC47464.1. AE014135 Genomic DNA. Translation: AAF59370.2. AE014135 Genomic DNA. Translation: AAF59371.2. AE014135 Genomic DNA. Translation: AAN06545.1. AE014135 Genomic DNA. Translation: AAN06546.1. AE014135 Genomic DNA. Translation: AAN06547.1. AE014135 Genomic DNA. Translation: AAN06548.1. AE014135 Genomic DNA. Translation: AAX52517.1. AE014135 Genomic DNA. Translation: ABC65830.1. BT021431 mRNA. Translation: AAX33579.1. BT029282 mRNA. Translation: ABK30919.1. AY312729 Genomic DNA. Translation: AAQ67543.1. AY312730 Genomic DNA. Translation: AAQ67544.1. AY312731 Genomic DNA. Translation: AAQ67545.1. AY312732 Genomic DNA. Translation: AAQ67546.1. AY312733 Genomic DNA. Translation: AAQ67547.1. AY312734 Genomic DNA. Translation: AAQ67548.1. AY312735 Genomic DNA. Translation: AAQ67549.1. AY312736 Genomic DNA. Translation: AAQ67550.1. AY312737 Genomic DNA. Translation: AAQ67551.1. AY312738 Genomic DNA. Translation: AAQ67552.1. AY312739 Genomic DNA. Translation: AAQ67553.1. AY312740 Genomic DNA. Translation: AAQ67554.1. AY312741 Genomic DNA. Translation: AAQ67555.1. AY312742 Genomic DNA. Translation: AAQ67556.1. AY312743 Genomic DNA. Translation: AAQ67557.1. AY312744 Genomic DNA. Translation: AAQ67558.1. AY312745 Genomic DNA. Translation: AAQ67559.1. AY312746 Genomic DNA. Translation: AAQ67560.1. AY312747 Genomic DNA. Translation: AAQ67561.1. AY312748 Genomic DNA. Translation: AAQ67562.1. AY312749 Genomic DNA. Translation: AAQ67563.1. AY312750 Genomic DNA. Translation: AAQ67564.1. AY312751 Genomic DNA. Translation: AAQ67565.1. AY312752 Genomic DNA. Translation: AAQ67566.1.
RefSeq	NP_001014685.1. NM_001014685.3. NP_001033798.1. NM_001038709.3. NP_001245406.1. NM_001258477.2. NP_524619.3. NM_079880.6. NP_726522.1. NM_166718.3. NP_726524.1. NM_166720.3. NP_726527.2. NM_166723.3.
UniGene	Dm.17803.
3D structure databases
ProteinModelPortal	P91943.
SMR	P91943. Positions 272-347.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19970N.
IntAct	P91943. 23 interactions.
MINT	MINT-994044.
Proteomic databases
PaxDb	P91943.
PRIDE	P91943.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0089156; FBpp0088223; FBgn0085432. FBtr0089157; FBpp0088224; FBgn0085432. FBtr0089158; FBpp0088225; FBgn0085432. FBtr0089159; FBpp0088226; FBgn0085432. FBtr0089160; FBpp0088227; FBgn0085432. FBtr0089161; FBpp0088228; FBgn0085432. FBtr0089162; FBpp0088229; FBgn0085432. FBtr0309803; FBpp0301556; FBgn0085432.
GeneID	43769.
KEGG	dme:Dmel_CG34403.
Organism-specific databases
CTD	43769.
FlyBase	FBgn0085432. pan.
Phylogenomic databases
eggNOG	NOG252916.
GeneTree	ENSGT00390000009964.
InParanoid	P91943.
OMA	LNSHPAI.
OrthoDB	EOG4W9GKB.
Enzyme and pathway databases
SignaLink	P91943.
Gene expression databases
Bgee	P91943.
GermOnline	CG17964. Drosophila melanogaster.
Family and domain databases
Gene3D	1.10.30.10. 1 hit. 4.10.900.10. 1 hit.
InterPro	IPR027397. Catenin_binding_dom. IPR013558. CTNNB1-bd_N. IPR009071. HMG_box_dom. IPR024940. TCF/LEF. [Graphical view]
PANTHER	PTHR10373. PTHR10373. 1 hit.
Pfam	PF08347. CTNNB1_binding. 1 hit. PF00505. HMG_box. 1 hit. [Graphical view]
SMART	SM00398. HMG. 1 hit. [Graphical view]
SUPFAM	SSF47095. HMG-box. 1 hit.
PROSITE	PS50118. HMG_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	pan. drosophila.
GenomeRNAi	43769.
NextBio	835691.

Entry information

Entry name

PANG1_DROME

Accession

Primary (citable) accession number: P91943
Secondary accession number(s): A0AVW4 , A4V148, O02548, Q0KIE9, Q59DP5, Q5BHZ3, Q6W492, Q6W493, Q6W494, Q6W495, Q6W4A6, Q6W4B4, Q9V4B0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	May 29, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project