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Protein

Tubulin alpha-3 chain

Gene

mec-12

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tubulin is the major constituent of microtubules (Probable). It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (Probable). Component of the large-diameter neuronal microtubules which contains 15 protofilaments (PubMed:9885292). Plays a role in mechanosensory transduction (touch sensitivity) (PubMed:9885292, PubMed:19652181).Curated2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei450Involved in polymerization1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi142 – 148GTPSequence analysis7

GO - Molecular functioni

  • GTPase activity Source: InterPro
  • GTP binding Source: UniProtKB-KW
  • structural constituent of cytoskeleton Source: WormBase

GO - Biological processi

  • detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • mechanosensory behavior Source: WormBase
  • microtubule-based process Source: WormBase
  • positive regulation of detection of mechanical stimulus involved in sensory perception of touch Source: UniProtKB
  • positive regulation of mechanosensory behavior Source: UniProtKB
  • response to mechanical stimulus Source: WormBase
  • thigmotaxis Source: WormBase

Keywordsi

LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-114608 Platelet degranulation
R-CEL-5620924 Intraflagellar transport
R-CEL-6807878 COPI-mediated anterograde transport
R-CEL-6811436 COPI-independent Golgi-to-ER retrograde traffic

Names & Taxonomyi

Protein namesi
Recommended name:
Tubulin alpha-3 chain
Alternative name(s):
Mechanosensory abnormality protein 12
Short name:
MEC-12
Cleaved into the following chain:
Gene namesi
Name:mec-12
Synonyms:tba-3
ORF Names:C44B11.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiC44B11.3 ; CE24843 ; WBGene00003175 ; mec-12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Microtubule

Pathology & Biotechi

Disruption phenotypei

Touch insensitive phenotype, which may be due to defective touch receptor neuron synaptic transmission.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40K → Q or R: Reduces the touch responsiveness of the worm. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004034771 – 450Tubulin alpha-3 chainAdd BLAST450
ChainiPRO_00004374031 – 449Detyrosinated tubulin alpha-3 chainBy similarityAdd BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei40N6-acetyllysine2 Publications1

Post-translational modificationi

Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.By similarity
Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP91910
PaxDbiP91910
PeptideAtlasiP91910
PRIDEiP91910

PTM databases

iPTMnetiP91910

Expressioni

Tissue specificityi

Specifically expressed in neurons.1 Publication

Gene expression databases

BgeeiWBGene00003175

Interactioni

Subunit structurei

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells.

Protein-protein interaction databases

STRINGi6239.C44B11.3

Structurei

3D structure databases

ProteinModelPortaliP91910
SMRiP91910
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tubulin family.Curated

Phylogenomic databases

eggNOGiKOG1376 Eukaryota
COG5023 LUCA
GeneTreeiENSGT00760000119060
HOGENOMiHOG000165711
InParanoidiP91910
KOiK07374
OMAiMYGKRAF
OrthoDBiEOG091G0736
PhylomeDBiP91910

Family and domain databases

Gene3Di1.10.287.600, 1 hit
3.30.1330.20, 1 hit
3.40.50.1440, 1 hit
InterProiView protein in InterPro
IPR002452 Alpha_tubulin
IPR008280 Tub_FtsZ_C
IPR000217 Tubulin
IPR018316 Tubulin/FtsZ_2-layer-sand-dom
IPR037103 Tubulin/FtsZ_C_sf
IPR036525 Tubulin/FtsZ_GTPase_sf
IPR023123 Tubulin_C
IPR017975 Tubulin_CS
IPR003008 Tubulin_FtsZ_GTPase
PANTHERiPTHR11588 PTHR11588, 1 hit
PfamiView protein in Pfam
PF00091 Tubulin, 1 hit
PF03953 Tubulin_C, 1 hit
PRINTSiPR01162 ALPHATUBULIN
PR01161 TUBULIN
SMARTiView protein in SMART
SM00864 Tubulin, 1 hit
SM00865 Tubulin_C, 1 hit
SUPFAMiSSF52490 SSF52490, 1 hit
SSF55307 SSF55307, 1 hit
PROSITEiView protein in PROSITE
PS00227 TUBULIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91910-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MREVISIHIG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK SLGGSDDSFS
60 70 80 90 100
TFFSETGSGR HVPRAVMVDL EPTVIDEIRT GTYRSLFHPE QLITGKEDAA
110 120 130 140 150
NNYARGHYTI GKEIIDLTLD RIRRLADNCT GLQGFLVFHS FGGGTGSGFT
160 170 180 190 200
SLLMERLSVD YGKKAKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC
210 220 230 240 250
SFMVDNEAIY DICRRNLDIE RPSYTNLNRL IGQIVSSITA SLRFDGALNV
260 270 280 290 300
DLTEFQTNLV PYPRIHFPLA TFSPVISAEK AYHEQLSVAE ITNMCFEPHN
310 320 330 340 350
QMVKCDPRHG KYMAVCLLFR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG
360 370 380 390 400
FKVGINYQPP TVVPGGDLAK VPRAVCMLSN TTAIAEAWAR LDHKFDLMYA
410 420 430 440 450
KRAFVHWYVG EGMEEGEFSE AREDLAALEK DYEEVGVDSM EDNGEEGDEY
Length:450
Mass (Da):50,113
Last modified:May 1, 1997 - v1
Checksum:iCA62ABC963771DB4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U65429 mRNA Translation: AAB48241.1
AB017190 mRNA Translation: BAA32600.1
D21134 Genomic DNA Translation: BAA22203.1
FO080849 Genomic DNA Translation: CCD67209.1
PIRiT15271
RefSeqiNP_497663.1, NM_065262.5
UniGeneiCel.17899

Genome annotation databases

EnsemblMetazoaiC44B11.3; C44B11.3; WBGene00003175
GeneIDi175419
KEGGicel:CELE_C44B11.3
UCSCiC44B11.3 c. elegans

Similar proteinsi

Entry informationi

Entry nameiTBA3_CAEEL
AccessioniPrimary (citable) accession number: P91910
Secondary accession number(s): O01907, Q7JQD4, Q9TYG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2011
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health