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P91887

- AMPN_PLUXY

UniProt

P91887 - AMPN_PLUXY

Protein

Aminopeptidase N

Gene

APN1

Organism
Plutella xylostella (Diamondback moth) (Plutella maculipennis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi344 – 3441Zinc; catalyticPROSITE-ProRule annotation
    Active sitei345 – 3451Proton acceptorPROSITE-ProRule annotation
    Metal bindingi348 – 3481Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi367 – 3671Zinc; catalyticPROSITE-ProRule annotation
    Sitei429 – 4291Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.030.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase N (EC:3.4.11.2)
    Short name:
    AP-N
    Alternative name(s):
    Apn1
    Microsomal aminopeptidase
    Gene namesi
    Name:APN1
    OrganismiPlutella xylostella (Diamondback moth) (Plutella maculipennis)
    Taxonomic identifieri51655 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaYponomeutoideaPlutellidaePlutella

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei? – 946Removed in mature formSequence AnalysisPRO_0000026744
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Chaini16 – ?Aminopeptidase NPRO_0000026743

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi60 – 601N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi550 – 5501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi605 – 6051N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi715 ↔ 722By similarity
    Disulfide bondi751 ↔ 787By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

    Structurei

    3D structure databases

    ProteinModelPortaliP91887.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni308 – 3125Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PfamiPF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P91887-1 [UniParc]FASTAAdd to Basket

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    MRLLICLTLL GLVCGNPVQL TDNSIALQNT YDNYVLPGES FPTFYDVQLF    50
    FDPEYEASFN GTVAIRVVPR IATQEIVLHA MEMEILSIRA YSDLPSDDNL 100
    NENLFSSYTL ATDDTHLLKI QFTRVLDALQ PITVEISYSA QYAPNMFGVY 150
    VSRYVENGAT VSLVTSQLQP TFARRAFPCY DEPALKAVFR TTIYAPPAYN 200
    VVETNMPLRT DSLKSDRPGF TKHEFQDTLV MSSYLLAYLV SKFDYISNEN 250
    NPTYDKSMKV FSRPGTQNTA EFALDFGQKN MVELEKYTEF PYAFPKIDKV 300
    AVPDFAAGAM ENWGLVIYRE IALLVQEGVT TTSTLQGIGR IISHENTHQW 350
    FGNEVGPDSW TYTWLNEGFA NFFESFATDL VLPEWRMMDQ FVINMQNVFQ 400
    SDAVLSVNPI TFEVRTPSQI LGTFNSVAYQ KSGSVIRMMQ HFLTPEIFRK 450
    SLALYISRMS RKAAKPTDLF EAIQEVVDAS DHSIRWRLSI IMNRWTQQGG 500
    FPVVTVRRSA PSAQSFVITQ RRFLTDSTQE SNTVWNVPLN WVLSTDVNFN 550
    DTRPMAWLPP QLAAEAVQVP GLQNAEWFIV NKQQTGYYRV NYDPENWRAL 600
    AKVLNDTHEI IHLLNRAQLI DDSFNLARNG RLDYSLAFDL SRYLVQERDY 650
    IPWAAANAAF NYLNSVLSGS SVHPLFQEYL LFLTAPLYQR LGFNAATGEE 700
    HVTPFHRNII LNINCLHGNE DCVSTAETLL QNFRDNPTQT LNPDIQTTVF 750
    CSGLRGGDVD NFNFLWARYT ATQDSSEQSI LLNALGCTSN ADRRDFLFSQ 800
    VIASDSQVRE QDRHSVLVSA INSGPDNMNA ALDFVLENFA NIQPNVQGLT 850
    GTTNILNAFA RTLTTQEHAN KIDEFSNKYA NVFTAGEMAS VAAIKENIAA 900
    SITWNSQNAA TVEAWLRKNF GTDGASTVSA SITIIISAMV AIYNIL 946
    Length:946
    Mass (Da):106,578
    Last modified:May 1, 1997 - v1
    Checksum:i1DFD81A364067BFB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97878 mRNA. Translation: CAA66467.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X97878 mRNA. Translation: CAA66467.1 .

    3D structure databases

    ProteinModelPortali P91887.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M01.030.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR024571. ERAP1-like_C_dom.
    IPR001930. Peptidase_M1.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    Pfami PF11838. ERAP1_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N related to Bacillus thuringiensis toxin-binding proteins."
      Denolf P.H., Hendrickx K., van Damme J., Jansens S., Peferoen M., Degheele D., van Rie J.
      Eur. J. Biochem. 248:748-761(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
      Tissue: Midgut.

    Entry informationi

    Entry nameiAMPN_PLUXY
    AccessioniPrimary (citable) accession number: P91887
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3