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P91887 (AMPN_PLUXY) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aminopeptidase N

Short name=AP-N
EC=3.4.11.2
Alternative name(s):
Apn1
Microsomal aminopeptidase
Gene names
Name:APN1
OrganismPlutella xylostella (Diamondback moth) (Plutella maculipennis)
Taxonomic identifier51655 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaLepidopteraGlossataDitrysiaYponomeutoideaPlutellidaePlutella

Protein attributes

Sequence length946 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Sequence similarities

Belongs to the peptidase M1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Chain16 – ?Aminopeptidase NPRO_0000026743
Propeptide? – 946Removed in mature form PotentialPRO_0000026744

Regions

Region308 – 3125Substrate binding By similarity

Sites

Active site3451Proton acceptor By similarity
Metal binding3441Zinc; catalytic By similarity
Metal binding3481Zinc; catalytic By similarity
Metal binding3671Zinc; catalytic By similarity
Site4291Transition state stabilizer By similarity

Amino acid modifications

Glycosylation601N-linked (GlcNAc...) Potential
Glycosylation5501N-linked (GlcNAc...) Potential
Glycosylation6051N-linked (GlcNAc...) Potential
Disulfide bond715 ↔ 722 By similarity
Disulfide bond751 ↔ 787 By similarity

Sequences

Sequence LengthMass (Da)Tools
P91887 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 1DFD81A364067BFB

FASTA946106,578
        10         20         30         40         50         60 
MRLLICLTLL GLVCGNPVQL TDNSIALQNT YDNYVLPGES FPTFYDVQLF FDPEYEASFN 

        70         80         90        100        110        120 
GTVAIRVVPR IATQEIVLHA MEMEILSIRA YSDLPSDDNL NENLFSSYTL ATDDTHLLKI 

       130        140        150        160        170        180 
QFTRVLDALQ PITVEISYSA QYAPNMFGVY VSRYVENGAT VSLVTSQLQP TFARRAFPCY 

       190        200        210        220        230        240 
DEPALKAVFR TTIYAPPAYN VVETNMPLRT DSLKSDRPGF TKHEFQDTLV MSSYLLAYLV 

       250        260        270        280        290        300 
SKFDYISNEN NPTYDKSMKV FSRPGTQNTA EFALDFGQKN MVELEKYTEF PYAFPKIDKV 

       310        320        330        340        350        360 
AVPDFAAGAM ENWGLVIYRE IALLVQEGVT TTSTLQGIGR IISHENTHQW FGNEVGPDSW 

       370        380        390        400        410        420 
TYTWLNEGFA NFFESFATDL VLPEWRMMDQ FVINMQNVFQ SDAVLSVNPI TFEVRTPSQI 

       430        440        450        460        470        480 
LGTFNSVAYQ KSGSVIRMMQ HFLTPEIFRK SLALYISRMS RKAAKPTDLF EAIQEVVDAS 

       490        500        510        520        530        540 
DHSIRWRLSI IMNRWTQQGG FPVVTVRRSA PSAQSFVITQ RRFLTDSTQE SNTVWNVPLN 

       550        560        570        580        590        600 
WVLSTDVNFN DTRPMAWLPP QLAAEAVQVP GLQNAEWFIV NKQQTGYYRV NYDPENWRAL 

       610        620        630        640        650        660 
AKVLNDTHEI IHLLNRAQLI DDSFNLARNG RLDYSLAFDL SRYLVQERDY IPWAAANAAF 

       670        680        690        700        710        720 
NYLNSVLSGS SVHPLFQEYL LFLTAPLYQR LGFNAATGEE HVTPFHRNII LNINCLHGNE 

       730        740        750        760        770        780 
DCVSTAETLL QNFRDNPTQT LNPDIQTTVF CSGLRGGDVD NFNFLWARYT ATQDSSEQSI 

       790        800        810        820        830        840 
LLNALGCTSN ADRRDFLFSQ VIASDSQVRE QDRHSVLVSA INSGPDNMNA ALDFVLENFA 

       850        860        870        880        890        900 
NIQPNVQGLT GTTNILNAFA RTLTTQEHAN KIDEFSNKYA NVFTAGEMAS VAAIKENIAA 

       910        920        930        940 
SITWNSQNAA TVEAWLRKNF GTDGASTVSA SITIIISAMV AIYNIL 

« Hide

References

[1]"Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N related to Bacillus thuringiensis toxin-binding proteins."
Denolf P.H., Hendrickx K., van Damme J., Jansens S., Peferoen M., Degheele D., van Rie J.
Eur. J. Biochem. 248:748-761(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
Tissue: Midgut.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X97878 mRNA. Translation: CAA66467.1.

3D structure databases

ProteinModelPortalP91887.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM01.030.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR024571. ERAP1-like_C_dom.
IPR001930. Peptidase_M1.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERPTHR11533. PTHR11533. 1 hit.
PfamPF11838. ERAP1_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSPR00756. ALADIPTASE.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAMPN_PLUXY
AccessionPrimary (citable) accession number: P91887
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: May 1, 1997
Last modified: May 14, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries