Reviewed, UniProtKB/Swiss-Prot P91875 (RPA1_DROME)

Names and origin

Protein names

Recommended name:
    DNA-directed RNA polymerase I subunit RPA1
      Short name=RNA polymerase I subunit A1
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I subunit A
    DNA-directed RNA polymerase I largest subunit

Gene names

Name:	RpI1
Synonyms:	RPA1
ORF Names:	CG10122

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1642 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Post-translational modification	Phosphorylated By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family. Contains 1 C2H2-type zinc finger.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1642	1642	DNA-directed RNA polymerase I subunit RPA1		PRO_0000073926
Regions
	Zinc finger	66 – 81	16	C2H2-type By similarity
	Region	939 – 951	13	Bridging helix By similarity
	Compositional bias	1333 – 1426	94	Asp-rich
Sites
	Metal binding	66	1	Zinc By similarity
	Metal binding	69	1	Zinc By similarity
	Metal binding	75	1	Zinc By similarity
	Metal binding	78	1	Zinc By similarity
	Metal binding	565	1	Magnesium; catalytic By similarity
	Metal binding	567	1	Magnesium; catalytic By similarity
	Metal binding	569	1	Magnesium; catalytic By similarity
Amino acid modifications
	Modified residue	1364	1	Phosphoserine Ref.5
	Modified residue	1365	1	Phosphoserine Ref.5
Experimental info
	Sequence conflict	407	1	S → C in CAA70321. Ref.1
	Sequence conflict	430	1	D → AY in CAA70321. Ref.1
	Sequence conflict	461 – 462	2	EL → DV in CAA70321. Ref.1
	Sequence conflict	758	1	L → V in CAA70321. Ref.1
	Sequence conflict	1042	1	A → V in CAA70321. Ref.1
	Sequence conflict	1085	1	I → V in CAA70321. Ref.1
	Sequence conflict	1315 – 1316	2	RA → PPP Ref.1
	Sequence conflict	1341	1	D → E in AAF58234. Ref.2
	Sequence conflict	1494	1	N → T in AAF58234. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P91875-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: B05DB5C4A5149F9A	FASTA	1,642	185,410
	10 20 30 40 50 60 MGSKRAMDVH MFPSDLEFAV FTDQEIRKLS VVKVITGITF DALGHAIPGG LYDIRMGSYG 70 80 90 100 110 120 RCMDPCGTCL KLQDCPGHMG HIELGTPVYN PFFIKFVQRL LCIFCLHCYK LQMKDHECEI 130 140 150 160 170 180 IMLQLRLIDA GYIIEAQELE LFKSEIVCQN TENLVAIKNG DMVHPHIAAM YKLLEKNEKN 190 200 210 220 230 240 SSNSTKTSCS LRTAITHSAL QRLGKKCRHC NKSMRFVRYM HRRLVFYVTL ADIKERVGTG 250 260 270 280 290 300 AETGGQNKVI FADECRRYLR QIYANYPELL KLLVPVLGLS NTDLTQGDRS PVDLFFMDTL 310 320 330 340 350 360 PVTPPRARPL NMVGDMLKGN PQTDIYINII ENNHVLNVVL KYMKGGQEKL TEEAKAAYQT 370 380 390 400 410 420 LKGETAHEKL YTAWLALQMS VDVLLDVNMS REMKSGEGLK QIIEKKSGLI RSHMMGKRVN 430 440 450 460 470 480 YAARTVITPD PNINVDEIGI PDIFAKKLSY PVPVTEWNVT ELRKMVMNGP DVHPGANYIQ 490 500 510 520 530 540 DKNGFTTYIP ADNASKRESL AKLLLSNPKD GIKIVHRHVL NGDVLLLNRQ PSLHKPSIMG 550 560 570 580 590 600 HKARILHGEK TFRLHYSNCK AYNADFDGDE MNAHYPQSEV ARAEAYNLVN VASNYLVPKD 610 620 630 640 650 660 GTPLGGLIQD HVISGVKLSI RGRFFNREDY QQLVFQGLSQ LKKDIKLLPP TILKPAVLWS 670 680 690 700 710 720 GKQILSTIII NIIPEGYERI NLDSFAKIAG KNWNVSRPRP PICGTNPEGN DLSESQVQIR 730 740 750 760 770 780 NGELLVGVLD KQQYGATTYG LIHCMYELYG GDVSTLLLTA FTKVFTFFLQ LEGFTLGVKD 790 800 810 820 830 840 ILVTDVADRK RRKIIRECRN VGNSAVAAAL ELEDEPPHDE LVEKMEAAYV KDSKFRVLLD 850 860 870 880 890 900 RKYKSLLDGY TNDINSTCLP RGLITKFPSN NLQLMVLSGA KGSMVNTMQI SCLLGQIELE 910 920 930 940 950 960 GKRPPLMISG KSLPSFTSFE TSPKSGGFID GRFMTGIQPQ DFFFHCMAGR EGLIDTAVKT 970 980 990 1000 1010 1020 SRSGYLQRCL IKHLEGLSVH YDLTVRDSDN SVVQFLYGED GLDILKSKFF NDKFCADFLT 1030 1040 1050 1060 1070 1080 QNATAILRPA QLQLMKDEEQ LAKVQRHEKH IRSWEKKKPA KLRAAFTHFS EELREEVEVK 1090 1100 1110 1120 1130 1140 RPNEINSKTG RRRFDEGLLK LWKKADAEDK ALYRKKYARC PDPTVAVYKQ DLYYGSVSER 1150 1160 1170 1180 1190 1200 TRKLITDYAK RKPALKETIA DIMRVKTIKS LAAPGEPVGL IAAQSIGEPS TQMTLNTFHF 1210 1220 1230 1240 1250 1260 AGRGEMNVTL GIPRLREILM LASSNIKTPS MDIPIKPGQQ HQAEKLRINL NSVTLANLLE 1270 1280 1290 1300 1310 1320 YVHVSTGLTL DPERSYEYDM RFQFLPREVY KEDYGVRPKH IIKYMHQTFF KQLIRAILKV 1330 1340 1350 1360 1370 1380 SNASRTTKIV VIDDKKDADK DDDNDLDNGD EVGRSKAKAN DDDSSDDNDD DDATGVKLKQ 1390 1400 1410 1420 1430 1440 RKTDEKDYDD PDDVEELHDA NDDDDEAEDE DDEEKGQDGN DNDGDDKAVE RLLSNDMVKA 1450 1460 1470 1480 1490 1500 YTYDKENHLW CQVKLNLSVR YQKPDLTSII RELAGKSVVH QVQHIKRAII YKGNDDDQLL 1510 1520 1530 1540 1550 1560 KTDGINIGEM FQHNKILDLN RLYSNDIHAI ARTYGIEAAS QVIVKEVSNV FKVYGITVDR 1570 1580 1590 1600 1610 1620 RHLSLIADYM TFDGTFQPLS RKGMEHSSSP LQQMSFESSL QFLKSAAGFG RADELSSPSS 1630 1640 RLMVGLPVRN GTGAFELLTK IC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the gene coding for the largest subunit of RNA polymerase I (A) of Drosophila melanogaster." Knackmuss S., Bautz E.K.F., Petersen G. Mol. Gen. Genet. 253:529-534(1997) [PubMed: 9065685] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Ovary.
[5]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1364 AND SER-1365, MASS SPECTROMETRY. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09103 Genomic DNA. Translation: CAA70321.1. AE013599 Genomic DNA. Translation: AAF58234.1. AY121632 mRNA. Translation: AAM51959.1.
PIR	T13803.
RefSeq	NP_523743.1.
UniGene	Dm.8031
3D structure databases
HSSP	HSSP built from PDB template 1K83 based on UniProtKB P04050.
ModBase	Search...
Protein-protein interaction databases
IntAct	P91875. 1 interaction.
Genome annotation databases
Ensembl	FBgn0019938. Drosophila melanogaster. [Contig view]
GeneID	36617.
KEGG	dme:Dmel_CG10122.
Organism-specific databases
FlyBase	FBgn0019938. RpI1.
Phylogenomic databases
HOGENOM	P91875.
Enzyme and pathway databases
BRENDA	2.7.7.6. 48.
Gene expression databases
ArrayExpress	P91875.
GermOnline	CG10122. Drosophila melanogaster.
Family and domain databases
InterPro	IPR015699. DNA-dir_RNA_pol1_lsu. IPR000910. HMG_HMG1/HMG2. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
Gene3D	G3DSA:1.10.30.10. HMG-box. 1 hit. G3DSA:3.90.1120.10. RNA_pol_Rpb1_1. 1 hit.
PANTHER	PTHR19376:SF11. RNA_polyI. 1 hit.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	799515.

Entry information

Entry name

RPA1_DROME

Accession

Primary (citable) accession number: P91875
Secondary accession number(s): Q8MRE2, Q9V732

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	December 7, 2004
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project