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Protein

Endoprotease aex-5

Gene

aex-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Probable serine endoprotease which cleaves preproteins at paired basic amino acids (PubMed:16945111). May process FMRFamide-like (flp) and neuropeptide-like protein (nlp) neuropeptides (PubMed:16945111). In muscles, involved in neuronal retrograde signaling by regulating presynaptic activity and localization of synaptic vesicle fusion protein unc-13 at the neuromuscular junction (NMJ) (PubMed:11804572). Acts in the intestine to regulate anterior body muscle contractions (aBOC) and the expulsion steps during the defecation motor program (DMP) (PubMed:2323555, PubMed:11804572, PubMed:18852466, PubMed:25849533). Probably by regulating DMP, required for fatty acid uptake by intestinal cells and therefore regulates the levels of triglycerides in the intestine (PubMed:25849533). Plays a role in locomotion (PubMed:11804572).5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei139Charge relay systemPROSITE-ProRule annotation1
Active sitei178Charge relay systemPROSITE-ProRule annotation1
Active sitei346Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • defecation Source: WormBase
  • lipid transport involved in lipid storage Source: UniProtKB
  • locomotion Source: WormBase
  • neuropeptide processing Source: UniProtKB
  • positive regulation of defecation Source: UniProtKB
  • positive regulation of defecation rhythm Source: UniProtKB
  • positive regulation of intestinal lipid absorption Source: UniProtKB
  • positive regulation of locomotion Source: UniProtKB
  • positive regulation of synaptic growth at neuromuscular junction Source: UniProtKB
  • positive regulation of triglyceride transport Source: UniProtKB
  • protein localization to synapse Source: WormBase
  • regulation of defecation Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

SignaLinkiP91863.

Protein family/group databases

MEROPSiS08.132.

Names & Taxonomyi

Protein namesi
Recommended name:
Endoprotease aex-5Curated (EC:3.4.21.-1 Publication)
Alternative name(s):
Subtilisin-like proteasePROSITE-ProRule annotation
Gene namesi
Name:aex-51 PublicationImported
Synonyms:kpc-3Imported
ORF Names:F32A7.6Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF32A7.6; CE28554; WBGene00000088; aex-5.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: UniProtKB

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes a reduction in fatty acid uptake (PubMed:25849533). RNAi-mediated knockdown in the intestine causes a severe defect in the expulsion step of the defecation cycle (PubMed:18852466).2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi184G → E in sv76; intestinal lipid droplets are smaller and their number is reduced. Reduced uptake and accumulation of triglycerides in the intestine. 1 Publication1
Mutagenesisi405G → S in sv75; intestinal lipid droplets are smaller and their number is reduced. Reduced uptake and accumulation of triglycerides in the intestine. 1 Publication1
Mutagenesisi443C → W in sa23; loss of production of several nlp and flp neuropeptides. Reduced enrichment of unc-13 at presynaptic active sites of neuromuscular junctions. Mutants are constipated due to a defect in body wall muscle and intestinal contractions and have a slightly longer defecation cycle. Reduced uptake and accumulation of triglycerides in the intestine. Mild body thrashing. 5 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000043945418 – 99Sequence analysisAdd BLAST82
ChainiPRO_5004161816100 – 537Endoprotease aex-5Sequence analysisAdd BLAST438

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi129N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi286 ↔ 316By similarity
Glycosylationi380N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP91863.

Expressioni

Gene expression databases

BgeeiWBGene00000088.

Interactioni

Protein-protein interaction databases

STRINGi6239.F32A7.6.

Structurei

3D structure databases

ProteinModelPortaliP91863.
SMRiP91863.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini132 – 394Peptidase S8Sequence analysisAdd BLAST263
Domaini407 – 537P/Homo BPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000021720.
InParanoidiP91863.
OMAiQRDIQHL.
OrthoDBiEOG091G09N7.
PhylomeDBiP91863.

Family and domain databases

CDDicd04059. Peptidases_S8_Protein_converta. 1 hit.
Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiView protein in InterPro
IPR008979. Galactose-bd-like.
IPR034182. Kexin/furin.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR002884. PrprotnconvertsP.
PfamiView protein in Pfam
PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
PROSITEiView protein in PROSITE
PS51829. P_HOMO_B. 1 hit.
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91863-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLIFLLLLF GVSPIVCQDF EDGVFLAKIT SIDEHDARKI GRRFGFEAQW
60 70 80 90 100
KLQSYTDVYV GRRLRRRKRG IEDEIVAEMM LSQQVQFIEK LQGFRRYKRA
110 120 130 140 150
PMTNKGYPVH VWNLTPSLYI REAWEDGFNA SRVTVAVVDD GVDIKHVDLK
160 170 180 190 200
SAFSPRVSFD FVRFGDLPTP KNSKEFEHGT QCAGLVAMEG QQCGLGVGHG
210 220 230 240 250
ATLGAIKLLG QDFLNDALEG DALAFQKDLI DIYSVSWGPK DDGKSAEKPA
260 270 280 290 300
KFTEEAIKNG ALHGRNGKGN IFVWASGNGG VNGDNCAYDG YVSNEYTLSF
310 320 330 340 350
GVIDASGAPA AYGEGCSSVL AAVSGGDAMI QTTGLESTCS SISGSSASAA
360 370 380 390 400
IASGIISLVL DANPTLSQRD IQHLIARTSN ASAIRDVELY ENSAGLNFHP
410 420 430 440 450
KVGFGLLNAQ KLVVMAATWE NVAPQVTCEK MNLANGIIDN SDCDVTKVER
460 470 480 490 500
VIVSGSIIHP HRGQVQIRLE SPRGTISELL PLRPKDTSRD LLDWNFVSVN
510 520 530
FFGENSRGIW KLHVTSEEDD VDFRVEMKMF KVVGTMS
Length:537
Mass (Da):58,705
Last modified:December 1, 2001 - v2
Checksum:i8160A7B53EF2D386
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CAB05501.2.
PIRiT21624.
RefSeqiNP_493594.1. NM_061193.5.
UniGeneiCel.38743.

Genome annotation databases

EnsemblMetazoaiF32A7.6; F32A7.6; WBGene00000088.
GeneIDi173358.
KEGGicel:CELE_F32A7.6.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CAB05501.2.
PIRiT21624.
RefSeqiNP_493594.1. NM_061193.5.
UniGeneiCel.38743.

3D structure databases

ProteinModelPortaliP91863.
SMRiP91863.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.F32A7.6.

Protein family/group databases

MEROPSiS08.132.

Proteomic databases

PaxDbiP91863.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF32A7.6; F32A7.6; WBGene00000088.
GeneIDi173358.
KEGGicel:CELE_F32A7.6.

Organism-specific databases

CTDi173358.
WormBaseiF32A7.6; CE28554; WBGene00000088; aex-5.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
COG1404. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000021720.
InParanoidiP91863.
OMAiQRDIQHL.
OrthoDBiEOG091G09N7.
PhylomeDBiP91863.

Enzyme and pathway databases

SignaLinkiP91863.

Gene expression databases

BgeeiWBGene00000088.

Family and domain databases

CDDicd04059. Peptidases_S8_Protein_converta. 1 hit.
Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiView protein in InterPro
IPR008979. Galactose-bd-like.
IPR034182. Kexin/furin.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR002884. PrprotnconvertsP.
PfamiView protein in Pfam
PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
PROSITEiView protein in PROSITE
PS51829. P_HOMO_B. 1 hit.
PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAEX5_CAEEL
AccessioniPrimary (citable) accession number: P91863
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: December 1, 2001
Last modified: May 10, 2017
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.