Reviewed, UniProtKB/Swiss-Prot P91856 (SERC_CAEEL)

Names and origin

Protein names

Recommended name:
    Probable phosphoserine aminotransferase
      Short name=PSAT
    EC=2.6.1.52
Alternative name(s):
    Phosphohydroxythreonine aminotransferase

Gene names

ORF Names:

F26H9.5

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity.
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate.
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. Cofactor biosynthesis; pyridoxine 5'-phosphate biosynthesis; pyridoxine 5'-phosphate from D-erythrose 4-phosphate: step 3/5.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Probable phosphoserine aminotransferase		PRO_0000150138
Regions
	Region	79 – 80	2	Pyridoxal phosphate binding By similarity
	Region	240 – 241	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	45	1	L-glutamate By similarity
	Binding site	105	1	Pyridoxal phosphate By similarity
	Binding site	154	1	Pyridoxal phosphate By similarity
	Binding site	175	1	Pyridoxal phosphate By similarity
	Binding site	198	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	199	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91856-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 676735A56AEEAFC1	FASTA	370	41,002
	10 20 30 40 50 60 MAAPGRKINF AAGPAKLPEE VLLKMQEEQL NFNNLGVSVI EMSHRSKEFG ALLNETISLI 70 80 90 100 110 120 RELMNVPDNF EILFMQGGGT GQFAAIPLNL KGDHEHADYI VTGAWSSKAA DEAGKYINVK 130 140 150 160 170 180 KVFQPSKPYV TVPDQENWVH DEKAAYLYYC ANETVHGIEF TPTAPESHNV PLVADVSSNF 190 200 210 220 230 240 MARPFDFKDH GVVFGGAQKN LGAAGLTIVI VRKDLIGKQQ AITPSVFSYK EMIANNSLYN 250 260 270 280 290 300 TPPTGGIYTT NLVLKWIKSK GGLQAIYELN LQKSGMIYDI IDNSNGFYHC AVDKRYRSIM 310 320 330 340 350 360 NVCFRIGGPS GNDELEEKFL KGSIERNMIS LKGHRSVGGI RASLYNAISV EETQVLATWM 370 NEFQKLHNTN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z81516 Genomic DNA. Translation: CAB04204.1.
PIR	T21441.
RefSeq	NP_492483.1.
UniGene	Cel.18863
3D structure databases
HSSP	HSSP built from PDB template 1BJN based on UniProtKB P23721.
ModBase	Search...
Protein-protein interaction databases
STRING	P91856.
Genome annotation databases
Ensembl	F26H9.5; F26H9.5; F26H9.5; Caenorhabditis elegans. [Genome view]
GeneID	172756.
KEGG	cel:F26H9.5.
NMPDR	fig|6239.3.peg.2470.
UCSC	F26H9.5. c. elegans.
Organism-specific databases
CTD	172756.
WormBase	WBGene00009177. F26H9.5.
WormPep	F26H9.5. CE09710. [WorfDB]
Phylogenomic databases
OMA	SMYNTPP.
Enzyme and pathway databases
BRENDA	2.6.1.52. 672.
Gene expression databases
ArrayExpress	P91856.
Family and domain databases
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR020578. Aminotrans_V_PyrdxlP_BS. IPR003248. Pser_amintransf. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
ProDom	PD001544. Pser_amintransf. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01364. serC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	876887.

Entry information

Entry name

SERC_CAEEL

Accession

Primary (citable) accession number: P91856

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 3, 2009
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project