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Protein

Macrophage migration inhibitory factor homolog

Gene

Bm1_28435

Organism
Brugia malayi (Filarial nematode worm)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Tautomerization of the methyl ester of L-dopachrome (By similarity). Inhibits migration of human peripheral blood mononuclear cells.By similarity1 Publication

Catalytic activityi

L-dopachrome = 5,6-dihydroxyindole-2-carboxylate.
Keto-phenylpyruvate = enol-phenylpyruvate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Proton acceptor; via imino nitrogenBy similarity
Binding sitei33 – 331SubstrateBy similarity
Binding sitei65 – 651Substrate; via amide nitrogenBy similarity

GO - Molecular functioni

  1. dopachrome isomerase activity Source: UniProtKB-EC
  2. phenylpyruvate tautomerase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Cytokine, Isomerase

Names & Taxonomyi

Protein namesi
Recommended name:
Macrophage migration inhibitory factor homolog (EC:5.3.2.1)
Short name:
Bm-MIF-1
Short name:
BmMIF
Short name:
MIF
Alternative name(s):
L-dopachrome isomerase
L-dopachrome tautomerase (EC:5.3.3.12)
Phenylpyruvate tautomerase
Gene namesi
ORF Names:Bm1_28435, BMBAC01P19.1
OrganismiBrugia malayi (Filarial nematode worm)
Taxonomic identifieri6279 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaSpiruridaFilarioideaOnchocercidaeBrugia

Subcellular locationi

Secreted 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 115114Macrophage migration inhibitory factor homologPRO_0000158073Add
BLAST

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UZ7model-A/B/C2-115[»]
ProteinModelPortaliP91850.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MIF family.Curated

Phylogenomic databases

InParanoidiP91850.
KOiK07253.
OMAiMGKPAQY.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91850-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYFTIDTNI PQNSISSAFL KKASNVVAKA LGKPESYVSI HVNGGQAMVF
60 70 80 90 100
GGSEDPCAVC VLKSIGCVGP KVNNSHAEKL YKLLADELKI PKNRCYIEFV
110
DIEASSMAFN GSTFG
Length:115
Mass (Da):12,320
Last modified:September 22, 2009 - v4
Checksum:iF4D2FE1B56630E73
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti114 – 1141F → L in AAB60943 (PubMed:9826378).Curated
Sequence conflicti114 – 1141F → L in AAC82502 (PubMed:9826378).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002699 Genomic DNA. Translation: AAB60943.1.
U88035 mRNA. Translation: AAC82502.1.
AL606837 Genomic DNA. Translation: CAC70155.1.
DS239369 Genomic DNA. Translation: EDP34019.1.
RefSeqiXP_001897136.1. XM_001897101.1.

Genome annotation databases

EnsemblMetazoaiBm6870a; Bm6870a; Bm6870.
GeneIDi6100584.
KEGGibmy:Bm1_28435.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF002699 Genomic DNA. Translation: AAB60943.1.
U88035 mRNA. Translation: AAC82502.1.
AL606837 Genomic DNA. Translation: CAC70155.1.
DS239369 Genomic DNA. Translation: EDP34019.1.
RefSeqiXP_001897136.1. XM_001897101.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1UZ7model-A/B/C2-115[»]
ProteinModelPortaliP91850.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiBm6870a; Bm6870a; Bm6870.
GeneIDi6100584.
KEGGibmy:Bm1_28435.

Organism-specific databases

CTDi6100584.

Phylogenomic databases

InParanoidiP91850.
KOiK07253.
OMAiMGKPAQY.

Family and domain databases

InterProiIPR001398. Macrophage_inhib_fac.
IPR019829. Macrophage_inhib_fac_CS.
IPR014347. Tautomerase/MIF_sf.
[Graphical view]
PANTHERiPTHR11954. PTHR11954. 1 hit.
PfamiPF01187. MIF. 1 hit.
[Graphical view]
ProDomiPD004816. Macrophage_inhib_fac. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF55331. SSF55331. 1 hit.
PROSITEiPS01158. MIF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Filarial nematode parasites secrete a homologue of the human cytokine macrophage migration inhibitory factor."
    Pastrana D.V., Raghavan N., Fitzgerald P., Eisinger S.W., Metz C., Bucala R., Schleimer R.P., Bickel C., Scott A.L.
    Infect. Immun. 66:5955-5963(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, SUBCELLULAR LOCATION.
    Strain: TRW.
  2. "Conservation of long-range and micro-synteny between the genomes of two distantly related nematodes."
    Gulliano D.B., Hall N., Jones S.J.M., Clark L.N., Corton C.H., Barrell B.G., Blaxter M.L.
    Genome Biol. 3:RESEARCH0057.1-RESEARCH0057.14(2001)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Draft genome of the filarial nematode parasite Brugia malayi."
    Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L.
    , Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J., Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W., Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M., McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., Blaxter M.L., Scott A.L.
    Science 317:1756-1760(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiMIFH_BRUMA
AccessioniPrimary (citable) accession number: P91850
Secondary accession number(s): A8PJU3, O02555, Q8IHI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: September 22, 2009
Last modified: April 1, 2015
This is version 68 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.