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Protein

Defensin-A

Gene

DEFA

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antibacterial peptide mostly active against Gram-positive bacteria. Has activity against the bacteria Gram-negative E.cloacae beta12.PROSITE-ProRule annotation3 Publications

GO - Biological processi

  • defense response to bacterium Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • innate immune response Source: UniProtKB

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Defensin
Biological processImmunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-A
Alternative name(s):
AaDef
Gene namesi
Name:DEFA
ORF Names:AAEL003841
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
PropeptideiPRO_000000673419 – 582 PublicationsAdd BLAST40
ChainiPRO_000000673559 – 98Defensin-AAdd BLAST40

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi61 ↔ 88PROSITE-ProRule annotation
Disulfide bondi74 ↔ 94PROSITE-ProRule annotation
Disulfide bondi78 ↔ 96PROSITE-ProRule annotation

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Developmental stagei

Expressed 75 minutes after bacterial infection. Increased dramatically after 6 hours, continued to increase through to 24 hours and is maintained at high levels until at least 30 hours after bacterial infection.2 Publications

Inductioni

By bacterial infection.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL003841-PA.

Structurei

3D structure databases

ProteinModelPortaliP91793.
SMRiP91793.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J8FB. Eukaryota.
ENOG410Z251. LUCA.
InParanoidiP91793.
OrthoDBiEOG091G1AEF.
PhylomeDBiP91793.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiView protein in InterPro
IPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
PfamiView protein in Pfam
PF01097. Defensin_2. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiView protein in PROSITE
PS51378. INVERT_DEFENSINS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSITVICFL ALCTVAITSA YPQEPVLADE ARPFANSLFD ELPEETYQAA
60 70 80 90
VENFRLKRAT CDLLSGFGVG DSACAAHCIA RGNRGGYCNS KKVCVCRN
Length:98
Mass (Da):10,583
Last modified:May 1, 1997 - v1
Checksum:i95F9457B90E66856
GO

Mass spectrometryi

Molecular mass is 4072.2 Da from positions 59 - 98. Determined by MALDI. 1 Publication

Polymorphismi

There are five defensin A isoforms, A1 (shown here) A2, A3, A4 and A5.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti2K → Q in isoforms A3, A4, A5 and A2 from strain Liverpool. 1
Natural varianti3S → P in isoform A4. 1
Natural varianti4I → L in isoforms A2, A3, A4 and A2 from strain Liverpool. 1
Natural varianti15V → G in isoforms A2, A3, A4, A5, B2, A1 from strain Liverpool and A2 from strain Liverpool. 1
Natural varianti16A → S in isoform B2, A1 from strain Liverpool. 1
Natural varianti24E → D in isoform A1 from strain Liverpool. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82860 mRNA. Translation: AAB46807.1.
S82861 mRNA. Translation: AAB46808.2.
S82862 mRNA. Translation: AAB46809.2.
S82863 mRNA. Translation: AAB46810.2.
AF156088 mRNA. Translation: AAD40112.1. Sequence problems.
AF156089 mRNA. Translation: AAD40113.1.
AF095259 mRNA. Translation: AAC64181.1.
AF387487 mRNA. Translation: AAK73080.1.
AF392802 Genomic DNA. Translation: AAL11701.1.
AF392803 Genomic DNA. Translation: AAL11702.1.
AF392804 Genomic DNA. Translation: AAL11703.1.
AY625500 Genomic DNA. Translation: AAT41586.1.
AY625501 Genomic DNA. Translation: AAT41587.1.
CH477283 Genomic DNA. Translation: EAT44833.1.
CH477283 Genomic DNA. Translation: EAT44837.1.
RefSeqiXP_001657289.1. XM_001657239.2.
XP_001657293.1. XM_001657243.2.
UniGeneiAae.5424.

Genome annotation databases

EnsemblMetazoaiAAEL003841-RA; AAEL003841-PA; AAEL003841.
GeneIDi5579095.
5579099.
KEGGiaag:AaeL_AAEL003841.
aag:AaeL_AAEL003857.
VectorBaseiAAEL003841-RA; AAEL003841-PA; AAEL003841.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDEFA_AEDAE
AccessioniPrimary (citable) accession number: P91793
Secondary accession number(s): O77250
, P91794, P91795, P91796, Q17EE3, Q6ITZ2, Q963E9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1997
Last modified: April 12, 2017
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families