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Protein

Defensin-A

Gene

DEFA

Organism
Aedes aegypti (Yellowfever mosquito) (Culex aegypti)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Antibacterial peptide mostly active against Gram-positive bacteria. Has activity against the bacteria Gram-negative E.cloacae beta12.PROSITE-ProRule annotation3 Publications

GO - Biological processi

  • defense response to bacterium Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • innate immune response Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-A
Alternative name(s):
AaDef
Gene namesi
Name:DEFA
ORF Names:AAEL003841
OrganismiAedes aegypti (Yellowfever mosquito) (Culex aegypti)
Taxonomic identifieri7159 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraNematoceraCulicoideaCulicidaeCulicinaeAediniAedesStegomyia
Proteomesi
  • UP000008820 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Propeptidei19 – 58402 PublicationsPRO_0000006734Add
BLAST
Chaini59 – 9840Defensin-APRO_0000006735Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi61 ↔ 88PROSITE-ProRule annotation
Disulfide bondi74 ↔ 94PROSITE-ProRule annotation
Disulfide bondi78 ↔ 96PROSITE-ProRule annotation

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Developmental stagei

Expressed 75 minutes after bacterial infection. Increased dramatically after 6 hours, continued to increase through to 24 hours and is maintained at high levels until at least 30 hours after bacterial infection.2 Publications

Inductioni

By bacterial infection.3 Publications

Interactioni

Protein-protein interaction databases

STRINGi7159.AAEL003841-PA.

Structurei

3D structure databases

ProteinModelPortaliP91793.
SMRiP91793. Positions 59-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the invertebrate defensin family. Type 1 subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J8FB. Eukaryota.
ENOG410Z251. LUCA.
InParanoidiP91793.
OrthoDBiEOG091G1AEF.
PhylomeDBiP91793.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P91793-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSITVICFL ALCTVAITSA YPQEPVLADE ARPFANSLFD ELPEETYQAA
60 70 80 90
VENFRLKRAT CDLLSGFGVG DSACAAHCIA RGNRGGYCNS KKVCVCRN
Length:98
Mass (Da):10,583
Last modified:May 1, 1997 - v1
Checksum:i95F9457B90E66856
GO

Mass spectrometryi

Molecular mass is 4072.2 Da from positions 59 - 98. Determined by MALDI. 1 Publication

Polymorphismi

There are five defensin A isoforms, A1 (shown here) A2, A3, A4 and A5.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti2 – 21K → Q in isoforms A3, A4, A5 and A2 from strain Liverpool.
Natural varianti3 – 31S → P in isoform A4.
Natural varianti4 – 41I → L in isoforms A2, A3, A4 and A2 from strain Liverpool.
Natural varianti15 – 151V → G in isoforms A2, A3, A4, A5, B2, A1 from strain Liverpool and A2 from strain Liverpool.
Natural varianti16 – 161A → S in isoform B2, A1 from strain Liverpool.
Natural varianti24 – 241E → D in isoform A1 from strain Liverpool.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82860 mRNA. Translation: AAB46807.1.
S82861 mRNA. Translation: AAB46808.2.
S82862 mRNA. Translation: AAB46809.2.
S82863 mRNA. Translation: AAB46810.2.
AF156088 mRNA. Translation: AAD40112.1. Sequence problems.
AF156089 mRNA. Translation: AAD40113.1.
AF095259 mRNA. Translation: AAC64181.1.
AF387487 mRNA. Translation: AAK73080.1.
AF392802 Genomic DNA. Translation: AAL11701.1.
AF392803 Genomic DNA. Translation: AAL11702.1.
AF392804 Genomic DNA. Translation: AAL11703.1.
AY625500 Genomic DNA. Translation: AAT41586.1.
AY625501 Genomic DNA. Translation: AAT41587.1.
CH477283 Genomic DNA. Translation: EAT44833.1.
CH477283 Genomic DNA. Translation: EAT44837.1.
RefSeqiXP_001657289.1. XM_001657239.2.
XP_001657293.1. XM_001657243.2.
UniGeneiAae.5424.

Genome annotation databases

EnsemblMetazoaiAAEL003841-RA; AAEL003841-PA; AAEL003841.
GeneIDi5579095.
5579099.
KEGGiaag:AaeL_AAEL003841.
aag:AaeL_AAEL003857.
VectorBaseiAAEL003841-RA; AAEL003841-PA; AAEL003841.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S82860 mRNA. Translation: AAB46807.1.
S82861 mRNA. Translation: AAB46808.2.
S82862 mRNA. Translation: AAB46809.2.
S82863 mRNA. Translation: AAB46810.2.
AF156088 mRNA. Translation: AAD40112.1. Sequence problems.
AF156089 mRNA. Translation: AAD40113.1.
AF095259 mRNA. Translation: AAC64181.1.
AF387487 mRNA. Translation: AAK73080.1.
AF392802 Genomic DNA. Translation: AAL11701.1.
AF392803 Genomic DNA. Translation: AAL11702.1.
AF392804 Genomic DNA. Translation: AAL11703.1.
AY625500 Genomic DNA. Translation: AAT41586.1.
AY625501 Genomic DNA. Translation: AAT41587.1.
CH477283 Genomic DNA. Translation: EAT44833.1.
CH477283 Genomic DNA. Translation: EAT44837.1.
RefSeqiXP_001657289.1. XM_001657239.2.
XP_001657293.1. XM_001657243.2.
UniGeneiAae.5424.

3D structure databases

ProteinModelPortaliP91793.
SMRiP91793. Positions 59-98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7159.AAEL003841-PA.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiAAEL003841-RA; AAEL003841-PA; AAEL003841.
GeneIDi5579095.
5579099.
KEGGiaag:AaeL_AAEL003841.
aag:AaeL_AAEL003857.
VectorBaseiAAEL003841-RA; AAEL003841-PA; AAEL003841.

Organism-specific databases

CTDi5579095.
5579099.

Phylogenomic databases

eggNOGiENOG410J8FB. Eukaryota.
ENOG410Z251. LUCA.
InParanoidiP91793.
OrthoDBiEOG091G1AEF.
PhylomeDBiP91793.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR001542. Defensin_invertebrate/fungal.
IPR003614. Scorpion_toxin-like.
[Graphical view]
PfamiPF01097. Defensin_2. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS51378. INVERT_DEFENSINS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEFA_AEDAE
AccessioniPrimary (citable) accession number: P91793
Secondary accession number(s): O77250
, P91794, P91795, P91796, Q17EE3, Q6ITZ2, Q963E9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.