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Protein

Xanthine dehydrogenase

Gene

Xdh

Organism
Drosophila subobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid (By similarity).By similarity

Catalytic activityi

Xanthine + NAD+ + H2O = urate + NADH.
Hypoxanthine + NAD+ + H2O = xanthine + NADH.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 2 [2Fe-2S] clusters.By similarity
  • FADBy similarity
  • Mo-molybdopterinBy similarityNote: Binds 1 Mo-molybdopterin (Mo-MPT) cofactor per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi48 – 481Iron-sulfur 1By similarity
Metal bindingi53 – 531Iron-sulfur 1By similarity
Metal bindingi56 – 561Iron-sulfur 1By similarity
Metal bindingi78 – 781Iron-sulfur 1By similarity
Metal bindingi118 – 1181Iron-sulfur 2By similarity
Metal bindingi121 – 1211Iron-sulfur 2By similarity
Metal bindingi153 – 1531Iron-sulfur 2By similarity
Metal bindingi155 – 1551Iron-sulfur 2By similarity
Binding sitei344 – 3441FADBy similarity
Binding sitei367 – 3671FADBy similarity
Binding sitei415 – 4151FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei433 – 4331FADBy similarity
Metal bindingi781 – 7811MolybdenumBy similarity
Metal bindingi812 – 8121Molybdenum; via carbonyl oxygenBy similarity
Binding sitei816 – 8161SubstrateBy similarity
Binding sitei894 – 8941SubstrateBy similarity
Metal bindingi926 – 9261Molybdenum; via amide nitrogenBy similarity
Binding sitei928 – 9281SubstrateBy similarity
Metal bindingi1093 – 10931Molybdenum; via amide nitrogenBy similarity
Active sitei1276 – 12761Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi264 – 2718FADBy similarity
Nucleotide bindingi354 – 3585FADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, FAD, Flavoprotein, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Xanthine dehydrogenase (EC:1.17.1.4)
Short name:
XD
Alternative name(s):
Protein rosy locus
Gene namesi
Name:Xdh
Synonyms:ry
OrganismiDrosophila subobscura (Fruit fly)
Taxonomic identifieri7241 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Organism-specific databases

FlyBaseiFBgn0013892. Dsub\Xdh.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13441344Xanthine dehydrogenasePRO_0000166081Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP91711.
SMRiP91711. Positions 11-170.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini9 – 96882Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini236 – 425190FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the xanthine dehydrogenase family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P91711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQQQATSV LVFFVNGKKV TDTNPDPECT LLTYLRDKLR LCGTKLGCAE
60 70 80 90 100
GGCGACTVMI SRMDRGQHKI RHLAVNACLT PVCAMHGCAV TTVEGIGSTR
110 120 130 140 150
TRLHPVQERL AKAHGSQCGF CTPGIVMSMY ALLRNAEQPS MRDLEVAFQG
160 170 180 190 200
NLCRCTGYRP ILEGYKTFTK EFLCGMGEKC CRVNGKGCGG GDDPESVTDD
210 220 230 240 250
TLFERSKFQP LDASQEPIFP PELQLSNAYD SESLVFSSER VTWYRPTTLQ
260 270 280 290 300
ELLQLKAAHP AAKLVVGNTE VGVEVKFKHF LYPHLINPTL VAELQEVRES
310 320 330 340 350
EESIYFGAAV SLMEIDALLR QRIEELPEAQ TRLFQCTVDM LHYFAGKQIR
360 370 380 390 400
NVACLGGNIM TGSPISDMNP VLTAAGARLE VASIVEGKIS QRTVHMGTGF
410 420 430 440 450
FTGYRRNVIE PQEVLLGIHF QKTTPDQHVV AFKQARRRDD DIAIVNAAVN
460 470 480 490 500
VRFEPKSNVV AEISMAFGGM APTTVLAPRT SQLMVKQPLD HQLLERVAES
510 520 530 540 550
LCGELPLAAS APGGMIAYRR ALVVSLIFKA YLAISSKLSE AGIIAGDAIP
560 570 580 590 600
PKERSGAELF HTPTLRSAQL FERVCSDQPV CDPIGRPEVH AAALKQATGE
610 620 630 640 650
AIYTDDIPRM DGELYLGFVL STKPRAKITK LDASAALALE GVHAFFSHKD
660 670 680 690 700
LTVHENEVGP VFHDEHVFAA GEVHCYGQIV GAVAADNKAL AQRASRLVRV
710 720 730 740 750
EYEDLSPVIV TIEQAIEHGS YFPDYPRYVT KGNMAEAFAQ AEHTYEGSCR
760 770 780 790 800
MGGQEHFYLE THAAVAVPRD SDELELFCST QHPSEVQKLV AHVTSLPAHR
810 820 830 840 850
VVCRAKRLGG GFGGKESRGI SVALPVALAA YRLRRPVRCM LDRDEDMLIT
860 870 880 890 900
GTRHPFLFKY KVAFSSDGLI TACDIECYNN AGWSMDLSFS VLERAMYHFE
910 920 930 940 950
NCYHIPNVRV GGWVCKTNLP SNTAFRGFGG PQGMFAGEHI IRDVARIVGR
960 970 980 990 1000
DVLDVMRLNF YRTGDTTHYN QQLEHFPIER CLDDCLTQSR YHERRAEIAK
1010 1020 1030 1040 1050
FNRENRWRKR GVAVIPTKYG IAFGVMHLNQ AGALLNVYGD GSVLLSHGGV
1060 1070 1080 1090 1100
EIGQGLNTKM IQCAARALGI PSELIHISET ATDKVPNTSP TAASVGSDIN
1110 1120 1130 1140 1150
GMAVLDACEK LNKRLAPIKE ALPQATWQEW INKAYFDRVS LSATGFYAMP
1160 1170 1180 1190 1200
GIGYHPETNP NARTYSYYTN GVGISVVEID CLTGDHQVLS TDIVMDIGSS
1210 1220 1230 1240 1250
INPAIDIGQI EGAFMQGYGL FTLEELMYSP QGMLYSRGPG MYKLPGFADI
1260 1270 1280 1290 1300
PGEFNVSLLT GAPNPRAVYS SKAVGEPPLF IGASAFFAIK EAIAAARQEH
1310 1320 1330 1340
GLTGDFPLEA PSTSARIRMA CQDKFTNLLE VPEAGSFTPW NIVP
Length:1,344
Mass (Da):147,254
Last modified:May 1, 1997 - v1
Checksum:i1DDB5BAC0E4C3175
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08237 Genomic DNA. Translation: CAA69405.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08237 Genomic DNA. Translation: CAA69405.1.

3D structure databases

ProteinModelPortaliP91711.
SMRiP91711. Positions 11-170.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

FlyBaseiFBgn0013892. Dsub\Xdh.

Family and domain databases

Gene3Di1.10.150.120. 1 hit.
3.10.20.30. 1 hit.
3.30.365.10. 6 hits.
3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
3.90.1170.50. 1 hit.
InterProiIPR002888. 2Fe-2S-bd.
IPR001041. 2Fe-2S_ferredoxin-type.
IPR006058. 2Fe2S_fd_BS.
IPR000674. Ald_Oxase/Xan_DH_a/b.
IPR016208. Ald_Oxase/xanthine_DH.
IPR008274. AldOxase/xan_DH_Mopterin-bd.
IPR012675. Beta-grasp_dom.
IPR005107. CO_DH_flav_C.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR002346. Mopterin_DH_FAD-bd.
IPR022407. OxRdtase_Mopterin_BS.
IPR014307. Xanthine_DH_ssu.
[Graphical view]
PfamiPF01315. Ald_Xan_dh_C. 1 hit.
PF02738. Ald_Xan_dh_C2. 1 hit.
PF03450. CO_deh_flav_C. 1 hit.
PF00941. FAD_binding_5. 1 hit.
PF00111. Fer2. 1 hit.
PF01799. Fer2_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000127. Xanthine_DH. 1 hit.
SMARTiSM01008. Ald_Xan_dh_C. 1 hit.
SM01092. CO_deh_flav_C. 1 hit.
[Graphical view]
SUPFAMiSSF47741. SSF47741. 1 hit.
SSF54292. SSF54292. 1 hit.
SSF54665. SSF54665. 1 hit.
SSF55447. SSF55447. 1 hit.
SSF56003. SSF56003. 1 hit.
SSF56176. SSF56176. 1 hit.
TIGRFAMsiTIGR02963. xanthine_xdhA. 1 hit.
PROSITEiPS00197. 2FE2S_FER_1. 1 hit.
PS51085. 2FE2S_FER_2. 1 hit.
PS51387. FAD_PCMH. 1 hit.
PS00559. MOLYBDOPTERIN_EUK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Synonymous substitutions in the Xdh gene of Drosophila: heterogeneous distribution along the coding region."
    Comeron J.M., Aguade M.
    Genetics 144:1053-1062(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiXDH_DROSU
AccessioniPrimary (citable) accession number: P91711
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.