Reviewed, UniProtKB/Swiss-Prot P91682 (SMO_DROME)

Names and origin

Protein names

Recommended name:
    Protein smoothened
Alternative name(s):
    dSMO
    SMOH
    Smooth

Gene names

Name:	smo
ORF Names:	CG11561

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1036 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Segment polarity protein required for correct patterning of every segment. G protein-coupled receptor that associates with the patched protein (ptc) to transduce the hedgehog (hh) signal through the activation of an inhibitory G-protein. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu).
Subcellular location	Membrane; Multi-pass membrane protein.
Developmental stage	Expressed at all developmental stages, though the levels vary.
Sequence similarities	Belongs to the G-protein coupled receptor Fz/Smo family. Contains 1 FZ (frizzled) domain.
Caution	It is uncertain whether Met-1, Met-9, Met-13 or Met-14 is the initiator.

Ontologies

Keywords
Cellular component	Membrane
Domain	Signal Transmembrane
Molecular function	Developmental protein G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	Bolwig's organ morphogenesis Inferred from mutant phenotype. Source: FlyBase G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW Wnt receptor signaling pathway Traceable author statement. Source: FlyBase anterior/posterior lineage restriction, imaginal disc Traceable author statement. Source: FlyBase blastoderm segmentation Ref.2 Inferred from mutant phenotype. Source: FlyBase eye-antennal disc morphogenesis Inferred from genetic interaction. Source: FlyBase negative regulation of S phase of mitotic cell cycle Inferred from mutant phenotype. Source: FlyBase ovarian follicle cell development Inferred from mutant phenotype. Source: FlyBase smoothened signaling pathway Inferred from mutant phenotype. Source: FlyBase somatic stem cell maintenance Inferred from mutant phenotype. Source: FlyBase wing disc anterior/posterior pattern formation Traceable author statement. Source: FlyBase
Cellular component	endosome Traceable author statement. Source: FlyBase integral to membrane Traceable author statement. Source: FlyBase plasma membrane Traceable author statement. Source: FlyBase
Molecular function	G-protein coupled receptor activity Traceable author statement. Source: FlyBase hedgehog receptor activity Non-traceable author statement. Source: FlyBase non-G-protein coupled 7TM receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8SXJ8	1	EBI-142245,EBI-91604
cos	O16844	1	EBI-142245,EBI-497208

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 1036	1005	Protein smoothened		PRO_0000013019
Regions
	Topological domain	32 – 258	227	Extracellular Potential
	Transmembrane	259 – 279	21	1 Potential
	Topological domain	280 – 287	8	Cytoplasmic Potential
	Transmembrane	288 – 308	21	2 Potential
	Topological domain	309 – 339	31	Extracellular Potential
	Transmembrane	340 – 360	21	3 Potential
	Topological domain	361 – 381	21	Cytoplasmic Potential
	Transmembrane	382 – 402	21	4 Potential
	Topological domain	403 – 421	19	Extracellular Potential
	Transmembrane	422 – 442	21	5 Potential
	Topological domain	443 – 469	27	Cytoplasmic Potential
	Transmembrane	470 – 490	21	6 Potential
	Topological domain	491 – 532	42	Extracellular Potential
	Transmembrane	533 – 553	21	7 Potential
	Topological domain	554 – 1036	483	Cytoplasmic Potential
	Domain	85 – 206	122	FZ
	Compositional bias	816 – 819	4	Poly-Ser
Amino acid modifications
	Modified residue	658	1	Phosphoserine Ref.6
	Modified residue	659	1	Phosphoserine Ref.6
	Modified residue	667	1	Phosphoserine Ref.6
	Modified residue	670	1	Phosphoserine Ref.6
	Modified residue	673	1	Phosphoserine Ref.6
	Modified residue	687	1	Phosphoserine Ref.6
	Modified residue	690	1	Phosphoserine Ref.6
	Modified residue	693	1	Phosphoserine Ref.6
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	213	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	419	1	N-linked (GlcNAc...) Potential
	Disulfide bond	90 ↔ 155		By similarity
	Disulfide bond	100 ↔ 148		By similarity
	Disulfide bond	139 ↔ 179		By similarity
	Disulfide bond	172 ↔ 194		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P91682-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 7797FC71A539A87A	FASTA	1,036	116,552
	10 20 30 40 50 60 MQYLNFPRMP NIMMFLEVAI LCLWVVADAS ASSAKFGSTT PASAQQSDVE LEPINGTLNY 70 80 90 100 110 120 RLYAKKGRDD KPWFDGLDSR HIQCVRRARC YPTSNATNTC FGSKLPYELS SLDLTDFHTE 130 140 150 160 170 180 KELNDKLNDY YALKHVPKCW AAIQPFLCAV FKPKCEKING EDMVYLPSYE MCRITMEPCR 190 200 210 220 230 240 ILYNTTFFPK FLRCNETLFP TKCTNGARGM KFNGTGQCLS PLVPTDTSAS YYPGIEGCGV 250 260 270 280 290 300 RCKDPLYTDD EHRQIHKLIG WAGSICLLSN LFVVSTFFID WKNANKYPAV IVFYINLCFL 310 320 330 340 350 360 IACVGWLLQF TSGSREDIVC RKDGTLRHSE PTAGENLSCI VIFVLVYYFL TAGMVWFVFL 370 380 390 400 410 420 TYAWHWRAMG HVQDRIDKKG SYFHLVAWSL PLVLTITTMA FSEVDGNSIV GICFVGYINH 430 440 450 460 470 480 SMRAGLLLGP LCGVILIGGY FITRGMVMLF GLKHFANDIK STSASNKIHL IIMRMGVCAL 490 500 510 520 530 540 LTLVFILVAI ACHVTEFRHA DEWAQSFRQF IICKISSVFE EKSSCRIENR PSVGVLQLHL 550 560 570 580 590 600 LCLFSSGIVM STWCWTPSSI ETWKRYIRKK CGKEVVEEVK MPKHKVIAQT WAKRKDFEDK 610 620 630 640 650 660 GRLSITLYNT HTDPVGLNFD VNDLNSSETN DISSTWAAYL PQCVKRRMAL TGAATGNSSS 670 680 690 700 710 720 HGPRKNSLDS EISVSVRHVS VESRRNSVDS QVSVKIAEMK TKVASRSRGK HGGSSSNRRT 730 740 750 760 770 780 QRRRDYIAAA TGKSSRRRES STSVESQVIA LKKTTYPNAS HKVGVFAHHS SKKQHNYTSS 790 800 810 820 830 840 MKRRTANAGL DPSILNEFLQ KNGDFIFPFL QNQDMSSSSE EDNSRASQKI QDLNVVVKQQ 850 860 870 880 890 900 EISEDDHDGI KIEELPNSKQ VALENFLKNI KKSNESNSNR HSRNSARSQS KKSQKRHLKN 910 920 930 940 950 960 PAADLDFRKD CVKYRSNDSL SCSSEELDVA LDVGSLLNSS FSGISMGKPH SRNSKTSCDV 970 980 990 1000 1010 1020 GIQANPFELV PSYGEDELQQ AMRLLNAASR QRTEAANEDF GGTELQGLLG HSHRHQREPT 1030 FMSESDKLKM LLLPSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the hedgehog signal." Alcedo J., Ayzenzon M., von Ohlen T., Noll M., Hooper J.E. Cell 86:221-232(1996) [PubMed: 8706127] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"smoothened encodes a receptor-like serpentine protein required for hedgehog signalling." van den Heuvel M., Ingham P.W. Nature 382:547-551(1996) [PubMed: 8700230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[5]	Carlson J.W., Booth B., Frise E., Park S., Wan K.H., Yu C., Celniker S.E. Submitted (DEC-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley.
[6]	"Phosphoproteome analysis of Drosophila melanogaster embryos." Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P. J. Proteome Res. 7:1675-1682(2008) [PubMed: 18327897] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-658; SER-659; SER-667; SER-670; SER-673; SER-687; SER-690 AND SER-693, MASS SPECTROMETRY. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U87613 Genomic DNA. Translation: AAC33180.1. AF030334 Genomic DNA. Translation: AAB84275.1. AE014134 Genomic DNA. Translation: AAF51518.2. BT053691 mRNA. Translation: ACK77608.1.
PIR	S71804.
RefSeq	NP_523443.1.
UniGene	Dm.244
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19956N.
IntAct	P91682. 6 interactions.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PRIDE	P91682.
Genome annotation databases
Ensembl	FBgn0003444. Drosophila melanogaster. [Contig view]
GeneID	33196.
KEGG	dme:Dmel_CG11561.
NMPDR	fig|7227.3.peg.66.
Organism-specific databases
FlyBase	FBgn0003444. smo.
Phylogenomic databases
HOGENOM	P91682.
OMA	P91682. REDIVCR.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-006681-MON.
Gene expression databases
ArrayExpress	P91682.
GermOnline	CG11561. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000539. Frizzled. IPR000024. Frizzled_Cys-rich. IPR015526. Frizzled_related. IPR017981. GPCR_2-like. [Graphical view]
Gene3D	G3DSA:1.10.2000.10. Frizzled_Cys-rich. 1 hit.
PANTHER	PTHR11309. Fz_related. 1 hit.
Pfam	PF01534. Frizzled. 1 hit. [Graphical view]
PRINTS	PR00489. FRIZZLED.
PROSITE	PS50038. FZ. 1 hit. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	782367.

Entry information

Entry name

SMO_DROME

Accession

Primary (citable) accession number: P91682
Secondary accession number(s): B7FNK1, Q9VPM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project