Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Peptide transporter family 1

Gene

yin

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Important role in absorption of dietary peptides. High-affinity transporter of alanylalanine. Dipeptide transport activity is proton dependent.1 Publication

Kineticsi

  1. KM=48.8 µM for alanylalanine1 Publication

    pH dependencei

    Optimum pH is 6.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • oligopeptide transport Source: FlyBase
    • protein transport Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Biological processi

    Peptide transport, Protein transport, Transport

    Enzyme and pathway databases

    ReactomeiR-DME-427975. Proton/oligopeptide cotransporters.

    Protein family/group databases

    TCDBi2.A.17.4.2. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Peptide transporter family 1
    Alternative name(s):
    Oligopeptide transporter 1
    Protein YIN
    Gene namesi
    Name:yin
    Synonyms:opt1
    ORF Names:CG44402
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    Proteomesi
    • UP000000803 Componenti: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0265575. yin.

    Subcellular locationi

    • Membrane 1 Publication; Multi-pass membrane protein 1 Publication

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei74 – 9421HelicalSequence analysisAdd
    BLAST
    Transmembranei104 – 12421HelicalSequence analysisAdd
    BLAST
    Transmembranei132 – 15221HelicalSequence analysisAdd
    BLAST
    Transmembranei173 – 19321HelicalSequence analysisAdd
    BLAST
    Transmembranei207 – 22721HelicalSequence analysisAdd
    BLAST
    Transmembranei334 – 35421HelicalSequence analysisAdd
    BLAST
    Transmembranei364 – 38421HelicalSequence analysisAdd
    BLAST
    Transmembranei597 – 61923HelicalSequence analysisAdd
    BLAST
    Transmembranei629 – 64921HelicalSequence analysisAdd
    BLAST
    Transmembranei659 – 67921HelicalSequence analysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of plasma membrane Source: FlyBase
    Complete GO annotation...

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 743743Peptide transporter family 1PRO_0000064312Add
    BLAST

    Proteomic databases

    PaxDbiP91679.

    Expressioni

    Tissue specificityi

    Expressed in thorax and abdomen of females: apical epithelial membranes of midgut, rectum, and reproductive tract. Also expressed in neuropil of the central nervous system, with elevated expression within the alpha- and beta-lobes of the mushroom bodies.2 Publications

    Developmental stagei

    Expressed both maternally and zygotically.1 Publication

    Gene expression databases

    BgeeiFBgn0015565.
    ExpressionAtlasiP91679. differential.
    GenevisibleiP91679. DM.

    Interactioni

    Protein-protein interaction databases

    BioGridi57859. 8 interactions.
    IntActiP91679. 2 interactions.
    MINTiMINT-278374.
    STRINGi7227.FBpp0070601.

    Structurei

    3D structure databases

    ProteinModelPortaliP91679.
    SMRiP91679. Positions 32-389, 589-687.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    GeneTreeiENSGT00530000062961.
    InParanoidiP91679.
    OrthoDBiEOG091G0M1S.
    PhylomeDBiP91679.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR004768. Oligopep_transport.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 2 hits.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00926. 2A1704. 1 hit.
    PROSITEiPS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform A (identifier: P91679-1) [UniParc]FASTAAdd to basket
    Also known as: Long

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MASEITNGKN GQNGKNGQKE ESDSQIAPPI PYPKSVAFII SNEFCERFNY
    60 70 80 90 100
    YGMRTILVLY LTNKLGYNEE TATVLFHTFT MLVYIFPLIG ALIADGWLGK
    110 120 130 140 150
    YKTILYLSLV YSLGAMVVSF GAVPLSGMPT KAVTVVGLLL IAIGTGGIKP
    160 170 180 190 200
    CVSAFGGDQF SLPAQSFQLA KFFSLFYFAI NAGSLISTTF TPILRADVHC
    210 220 230 240 250
    FGDQDCFSLA FGVPAILMIF SVIIFMAGKR LYRCQPPAGN MIFGVSRCIA
    260 270 280 290 300
    DAFKGWQKRR HSEPMESFLD YAKPTVGSRM VQETKCLGRI LRLFLPFPVF
    310 320 330 340 350
    WALFDQQGSR WTFQATRMDG NVLGFQIKPD QMQVVNPLLI LGFLPLFDYI
    360 370 380 390 400
    IYPALARCGI RRPLQKLTLG LLLAALGFFL SAGLEMKMEQ AAYRATPIEP
    410 420 430 440 450
    DMTHLRIYNG MPCRYEISSA VVQTPRVIEP LNVWEDLSLQ MTESKEYTFN
    460 470 480 490 500
    AQPVSGECPS IIDKLRLQPG KSVSYFLAQD KLVEFADGLQ MAATDTGRTS
    510 520 530 540 550
    VRALLNTPDG EGPVLLSTES ATSQEPPLTL DKGNVPQLHR ITPGFARVDI
    560 570 580 590 600
    NGKKVASFEA KEGRLYSILV TGSARDGYQH NVIEVVALST VSILWQLPQI
    610 620 630 640 650
    VVMTAAEVMF SVTGLEFSYS QSPPSMKSVL QACWLLSVAI GNMLVVVIAE
    660 670 680 690 700
    FKFTSSQSGE FTLFASLMLV DMMIFLWLAR SYQYKDQRED FEDDDDATID
    710 720 730 740
    SVMQSKPKAT TVDTTARKTN GIEAEPGYGA YRNHAYDNDF SEA
    Length:743
    Mass (Da):82,245
    Last modified:September 13, 2005 - v2
    Checksum:iA382230B0DFE3107
    GO
    Isoform B (identifier: P91679-2) [UniParc]FASTAAdd to basket
    Also known as: Short

    The sequence of this isoform differs from the canonical sequence as follows:
         1-27: MASEITNGKNGQNGKNGQKEESDSQIA → MAFVSNAIVGPENCKSVTVSL

    Show »
    Length:737
    Mass (Da):81,576
    Checksum:i9F81329804365A23
    GO
    Isoform C (identifier: P91679-3) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-27: MASEITNGKNGQNGKNGQKEESDSQIA → MAFVSNAIVGPENCKSVT

    Note: No experimental confirmation available.
    Show »
    Length:734
    Mass (Da):81,276
    Checksum:iCB8ABC5EC6FBB20E
    GO

    Sequence cautioni

    The sequence AAL13696 differs from that shown. Reason: Erroneous initiation. Curated
    The sequence ABY21723 differs from that shown. Reason: Erroneous initiation. Curated

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti145 – 1451T → N in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti149 – 1491K → N in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti154 – 1541A → P in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti158 – 1581D → E in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti371 – 3711L → F in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti462 – 4621I → T in CAA20015 (PubMed:10731137).Curated
    Sequence conflicti588 – 5881L → P in AAC47465 (PubMed:9038337).Curated
    Sequence conflicti588 – 5881L → P in AAD04200 (PubMed:9730971).Curated
    Sequence conflicti588 – 5881L → P in AAD04201 (PubMed:9730971).Curated
    Sequence conflicti588 – 5881L → P in CAA20015 (PubMed:10731137).Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2727MASEI…DSQIA → MAFVSNAIVGPENCKSVTVS L in isoform B. 2 PublicationsVSP_015662Add
    BLAST
    Alternative sequencei1 – 2727MASEI…DSQIA → MAFVSNAIVGPENCKSVT in isoform C. 1 PublicationVSP_015663Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U85982 mRNA. Translation: AAC47465.1.
    U97114 Genomic DNA. Translation: AAD04200.1.
    U97114 Genomic DNA. Translation: AAD04201.1.
    AE014298 Genomic DNA. Translation: AAF45899.1.
    AE014298 Genomic DNA. Translation: AAF45900.2.
    AE014298 Genomic DNA. Translation: AAN09110.1.
    AL031130 Genomic DNA. Translation: CAA20014.1.
    AL031130 Genomic DNA. Translation: CAA20015.1.
    BT031266 mRNA. Translation: ABY20507.1.
    BT031310 mRNA. Translation: ABY21723.1. Different initiation.
    AY058467 mRNA. Translation: AAL13696.1. Different initiation.
    PIRiT13673.
    T13674.
    RefSeqiNP_477147.2. NM_057799.4. [P91679-1]
    NP_477148.2. NM_057800.4. [P91679-2]
    NP_726894.1. NM_166993.2. [P91679-3]
    UniGeneiDm.6966.

    Genome annotation databases

    EnsemblMetazoaiFBtr0340204; FBpp0309179; FBgn0265575. [P91679-1]
    GeneIDi31340.
    KEGGidme:Dmel_CG44402.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U85982 mRNA. Translation: AAC47465.1.
    U97114 Genomic DNA. Translation: AAD04200.1.
    U97114 Genomic DNA. Translation: AAD04201.1.
    AE014298 Genomic DNA. Translation: AAF45899.1.
    AE014298 Genomic DNA. Translation: AAF45900.2.
    AE014298 Genomic DNA. Translation: AAN09110.1.
    AL031130 Genomic DNA. Translation: CAA20014.1.
    AL031130 Genomic DNA. Translation: CAA20015.1.
    BT031266 mRNA. Translation: ABY20507.1.
    BT031310 mRNA. Translation: ABY21723.1. Different initiation.
    AY058467 mRNA. Translation: AAL13696.1. Different initiation.
    PIRiT13673.
    T13674.
    RefSeqiNP_477147.2. NM_057799.4. [P91679-1]
    NP_477148.2. NM_057800.4. [P91679-2]
    NP_726894.1. NM_166993.2. [P91679-3]
    UniGeneiDm.6966.

    3D structure databases

    ProteinModelPortaliP91679.
    SMRiP91679. Positions 32-389, 589-687.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi57859. 8 interactions.
    IntActiP91679. 2 interactions.
    MINTiMINT-278374.
    STRINGi7227.FBpp0070601.

    Protein family/group databases

    TCDBi2.A.17.4.2. the proton-dependent oligopeptide transporter (pot/ptr) family.

    Proteomic databases

    PaxDbiP91679.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblMetazoaiFBtr0340204; FBpp0309179; FBgn0265575. [P91679-1]
    GeneIDi31340.
    KEGGidme:Dmel_CG44402.

    Organism-specific databases

    CTDi31340.
    FlyBaseiFBgn0265575. yin.

    Phylogenomic databases

    eggNOGiKOG1237. Eukaryota.
    COG3104. LUCA.
    GeneTreeiENSGT00530000062961.
    InParanoidiP91679.
    OrthoDBiEOG091G0M1S.
    PhylomeDBiP91679.

    Enzyme and pathway databases

    ReactomeiR-DME-427975. Proton/oligopeptide cotransporters.

    Miscellaneous databases

    GenomeRNAii31340.
    PROiP91679.

    Gene expression databases

    BgeeiFBgn0015565.
    ExpressionAtlasiP91679. differential.
    GenevisibleiP91679. DM.

    Family and domain databases

    CDDicd06174. MFS. 1 hit.
    InterProiIPR020846. MFS_dom.
    IPR004768. Oligopep_transport.
    IPR000109. POT_fam.
    IPR018456. PTR2_symporter_CS.
    [Graphical view]
    PANTHERiPTHR11654. PTHR11654. 2 hits.
    PfamiPF00854. PTR2. 2 hits.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 2 hits.
    TIGRFAMsiTIGR00926. 2A1704. 1 hit.
    PROSITEiPS01022. PTR2_1. 1 hit.
    PS01023. PTR2_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiPEPT1_DROME
    AccessioniPrimary (citable) accession number: P91679
    Secondary accession number(s): A9UNA2
    , A9UNE6, O77281, O77282, Q7K7E7, Q7KVX8, Q8IRT2, Q95TX0, Q9TY25, Q9TY26, Q9W4P7, Q9W4P8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: September 13, 2005
    Last modified: September 7, 2016
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.